Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	335,016	C→T	G3R (GGA→AGA)	yahC ←	uncharacterized protein YahC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	335,016	0	C	T	100.0%	63.7 / NA	20	G3R (GGA→AGA)	yahC	uncharacterized protein YahC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/12);  total (8/12)

AACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCAGACTTCTGATAAACCCCGATTTACCGCCGCCTGCGGCATAAAAACTGCAAAAGGCGACGAAGCCAATGGTTCCTAATAATTCCCATCCTTGAGAGAGCCCGACATGTGAAGACACTAACTGCCAAAGCCCTGCACAAATACCGACGGTGACACCCGTTGCCGTGAGTCCGTTCATCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTACTCTTACATGTGCAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCAC  >  NC_000913/334791‑335260                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     aaCCAGCAGTCAGCGCGGCGAAAAACTCCCACACCATCCCGGAATAATTAACCGCCAGACTTCTGATAAACCCCGATTTACCGCCGCCTGCGGCATAAAAACTGCAAAAGGCGACGAAGCCAATGGTTCCTAATAATTCCCATCCTTGAGAGAGCCCGACATGTGAAGACACTAACTGCCAAAGCCCTGCACAAATACCGACGGTGACACCCGTTGCCGTGAGTCTGTTCATCTTGTTCCCCTCTGGTTAg                                                                                                                                                                                                                             <  2:153559/251‑1 (MQ=255)                cgacgAAAAACGCCACCCCCCTCCCGGAATAATTAACCGCCAGACTTCTGATAAAACCCGATTTACCTCCACCTGCGGCATAAAAACTGCAAAAGGCGACGAAGCCAATGGTTCCTAATAATTCCCATCCTTGAGAGAGCCCTACATGTTAAGACACTAACTGCCAAAGCTCTGCACAAATACCGACGGTGTCACCAGTTGCCGTGAGTCTGTTCATCGTGTTACCTCCTTGTTAGACATGGACGATAGCt                                                                                                                                                                                                              <  2:162937/248‑1 (MQ=255)                              caccccATCCCGGAACAATTAACCGCCAGACTTCTGATAAACCCCGATTTCCCGCCGCCTGCGGCATAAAAACTGCAAAAGGCGACGAAGCCAATGGTTCCTAATAATCCCCATCCTTGAGAGAGCCCGACATGTGAAGACACTAACTGCCAAAGCCCTGCACAAATACCGACGGTGACACCCTTTGCCGTGAGTCTGTTCATCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTACTCTTACATGTGc                                                                                                                                                                                                <  2:106507/247‑1 (MQ=255)                                 acCATCCCGGAATAATTAAACGCCAGACTTCTGATAAACCACGATTTACCGCCGCCTGCGGCATAAAAACTGCAAAAAGCGACGAAGCCAATGGTTCCTACTAATTCCCATCCTTGAGAGAGCCCGACATGTGAAGACACTATCTGCCAATGCCCTGCACAAATACCGACGGTGACACCCGTTGCCGTGAGTCTGTTCATCTTGTTCACCTCTGGTTAGACATCGACGAAAGCTACTCTTActtgatc                                                                                                                                                                                                >  2:75153/1‑242 (MQ=255)                                            ataatTAACCGCCAGACTTCTGATAAACCCCGATTTACCGCCGCCTGCGGCATAAAAACTGCAAAAGGCGACGAAGCCAATGGTTCCTAATAATTCCCATCCTTGAGAGAGCCCGACATGTGAAGACACTAACTGCCAAAGCCCTGCACAAATACCGACGGTGACACCCGTTGCCGTGAGTCTGTTCATCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTACTCTTACAGGTGCAGACTATAGAGaaa                                                                                                                                                                                  <  2:123204/251‑1 (MQ=255)                                               atcagcggCCAGACTTCTGATAAACCCCGATTTACCGCCGCCTGCGGCATAAAAACTGCAAAAGGCGACGAAGCGAATGGTGCCTAATAAGACCCAACCTTGAGAGAGCCCGACATGTGAAGACACTAACTGCCAAAGCCCTGCACAAATACCGTCGGTGACTCCCGTTGCCGTGAGTCTGTTCATCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTACTCTTACATGTGCAGAGTATAGAGAAATaa                                                                                                                                                                               <  1:48657/244‑1 (MQ=255)                                                     cGCCAGACTTCTGATAAACCCCGATTTACCGCCGCCTGCGGCATAAAAACTGCAAAAGGCGACGAAGCCAATGGTTCCTAATAATTCCCATCCTTGAGAGAGCCCGACATGTGAAGACACTAACTGCCAAAGCCCTGCACAAATACCGACGGTGACACCCGTTGCCGTGAGTCTGTTCATCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTACTCTTACATGTGCAGACTATAGAGAAATAAAACCTg                                                                                                                                                                         >  1:219895/1‑251 (MQ=255)                                                     cGCCAGACTTCTGATAAACCCCGATTTACCGCCGCCGGCGGCATAAAAACTGCAAAAGGCGACGAAGCCAATGGTTCCTAATAATTCCCATCCTTGAGAGAGCCCGACATGTGAAGACACTAACTGCCAAAGCCCTGCACAAATACCGACGGTGACACCCGTTGCCGTGAGTCTGTTCATCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTACTCTTACATGTGCAGACTATAGAGAAATAAAACCTg                                                                                                                                                                         <  2:200398/251‑1 (MQ=255)                                                      gCCAGACTTCTGATAAACCCCGATTTACCGCCGCCTGCGGCATAAAAACTGCAAAAGGCGACGAAGCCAATGGTTCCTAATAATTCCCATCCTTGAGAGAGCCCGACATGTGAAGACACTAACTGCCAAAGCCCTGCACAAATACCGACGGTGACACCCGTTGCCGTGAGTCTGTTCATCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTACTCTTACATGTGCAGACTATAGAGAAATAAAACCTg                                                                                                                                                                         >  1:157381/1‑250 (MQ=255)                                                                              ttACCGCCGCCTGCGGCATAAAAACTGCAAAAGGCGACGAAGCCAATGGTTCCTAATAATTCCCATCCTTGAGAGAGCCCGACATGTGAAGACACTAACTGCCAAAGCCCTGCACAAATACCGACGGTGACACCCGTTGCCGTGAGTCTGTTCATCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTACTCTTACATGTGCAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCa                                                                                                                                                 <  2:219895/250‑1 (MQ=255)                                                                                          gCGGCATAAAAACTGCAACAGGCGACGAAGCCAATGGTTCATAATAATTCCCATCCTTGAGAGAGCCCGACATGTGAAGACACTAACTGCCAAAGCCCTGCACAAATACCGACGGAGACCCCCGTTGCCGAGAGTCTGTTCCTCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTCCTCTTACATGTGCAGACTATAGAGAAATAAAACCTGCTTGAGTGAAAGGGTATCGGGTCAAAGAAACAAatat                                                                                                                                    >  2:107675/1‑251 (MQ=255)                                                                                                        gCAAAAGGCGACGAAGCCAATGGTTCCTAATAATTCCCATCCTTGAGAGAGCCCGACATGTGAAGACACTAACTGCCAAAGCCCTGCACAAATACCGACGGTGACACCCGTTGCCGTGAGTCTGTTCATCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTACTCTTACATGTGCAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGcac                                                                                                                       <  2:237908/250‑1 (MQ=255)                                                                                                          aaaaGGCGACGAAGCCAATGGTTCCTAATAATTCCCCTCCTTGAGAGAGCCCGACATGTGAAGACACTAACTGCCAAAGCCCTGCACAAATACCGACGGTGACACCCGTTGCCGTGAGTCTGTTCATCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTACTCTTACATGTGCAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACt                                                                                                                    >  2:100361/1‑251 (MQ=255)                                                                                                                                           cccAACCTTGAGAGAGCCCGACATGTGAAGACACTAACTGCCAAAGCCCTGCACAAATACCGACGGTGACACCCGTTGCCGTGAGTCTGTTCATCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTACTCTTACATGTGCAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACaaaaa                                                                                   <  2:157381/251‑1 (MQ=255)                                                                                                                                                            ccGACATGGGAAGACACTACCTGCCAAAGCCCTGCACAAATACCGACGGTGACACCCGTTGCCGTGAGTATGTTCATCTTGTTCCCCTCTGGTTAGACATGGACGACAGCTACTCTTACATGTGCATACTATAGAGTAATAAAACCTGATTGAGTGACATGGTATCGGTTCAAAGAAACAAATATTGCACTACCGCACACTGCGACACGATTGTTGAATATTATTcatgacataggtgagataaggcgaac                                                                  >  2:67333/1‑227 (MQ=255)                                                                                                                                                               aCATGTGAAGACACTAACTGCCAAAGCCCTGCACAAATACCGACGGTGACACCCGTTGCCGTGAGTCTGTTCATCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTACTCTTACATGTGCAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTa                                                                >  1:141203/1‑250 (MQ=255)                                                                                                                                                                 aggtgAAGACACTAACTGCCAAATCCCTGCACAAATACCGACGGTGACAGCCGTTGCCGTGAGTCTGTTCAGCTTGTTCCCCTCTTGTTAGACATGGACGAAAGCTACTCTTACATGTGCAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGTAAAGATTGTGGAATATGATACAACATAAATGTTAGTTACGGTGAATTAAAt                                                             <  2:234535/249‑1 (MQ=255)                                                                                                                                                                                     aaaGCCCTGCACAAATACCGACGGTGACACCCGTTGCCGTGAGTCTGTTCACCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTACTCTTACATGTGCAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATc                                          >  1:236662/1‑250 (MQ=255)                                                                                                                                                                                               acaAATACCGACGGTGACACCCGTTGCCGTGAGTCTGTTCATCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTACTCTTACATGTGCAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGtt                               <  2:127537/251‑1 (MQ=255)                                                                                                                                                                                                                             gAGTCTGTTCATCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTACTCTTACATGTGCAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCcac  <  1:102047/250‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     AACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCAGACTTCTGATAAACCCCGATTTACCGCCGCCTGCGGCATAAAAACTGCAAAAGGCGACGAAGCCAATGGTTCCTAATAATTCCCATCCTTGAGAGAGCCCGACATGTGAAGACACTAACTGCCAAAGCCCTGCACAAATACCGACGGTGACACCCGTTGCCGTGAGTCCGTTCATCTTGTTCCCCTCTGGTTAGACATGGACGAAAGCTACTCTTACATGTGCAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCAC  >  NC_000913/334791‑335260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑