Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	334,180	C→T	R85K (AGA→AAA)	yahB ←	putative LysR‑type DNA‑binding transcriptional regulator YahB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	334,180	0	C	T	100.0%	59.6 / NA	17	R85K (AGA→AAA)	yahB	putative LysR‑type DNA‑binding transcriptional regulator YahB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/5);  total (12/5)

ATACCGCCGCCGTCCAGTAGTGTATCGGGTGCGCCGATGGCGATATTGGCCTGATTATTAATAATCGCATCCCAGACGCCGTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGCGCAGCGGTAGTAAATGCCAACAGATTCTCTT  >  NC_000913/333949‑334414                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           aTACCGCCGCCGTCCAGTAGTGTATCGGGTGCGCCGATGGCGATATTGGCCTGATTATTAATAATCGCATCCCAGACGCCGTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTTTGCGTTTGATGGCATaaaa                                                                                                                                                                                                                         <  2:210950/251‑1 (MQ=255)                         tCGGGTGCGCCGATGGCGATATTGGCCTGATTATTAATAATCGCATCCCAGACGCCGTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTTTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGt                                                                                                                                                                                                       <  2:219223/245‑1 (MQ=255)                         tCGGGTGCGCCGATGGCGATATTGGCCTGATTATTAATAATCGCATCCCAGACGCCGTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTTTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGt                                                                                                                                                                                                       >  1:219223/1‑245 (MQ=255)                                   cGATGGCGATATTGGCCTGATTATTAATAATCGCATCCCAGACGCCGTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTTTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGaaaaa                                                                                                                                                                                        >  2:223212/1‑250 (MQ=255)                                                       ttattaATAATCGCATCCCAGACGCCGTTATACACTTCGGTGGTGACGGTAATCTGGCAGGGAGGAAACTGCTTTTTCAGCACCTGTAACAAGAGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTTTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTa                                                                                                                                                                    >  1:78000/1‑250 (MQ=255)                                                          ttaATAATCGCATCCCAGACGCCGTTATACACTTCGGTGGTGAAGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGGGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTTTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTc                                                                                                                                                                >  1:54367/1‑251 (MQ=255)                                                                                    ataCACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTTTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAAc                                                                                                                                       >  1:150472/1‑250 (MQ=255)                                                                                                     cGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTTTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGcccc                                                                                                                     <  1:139685/251‑1 (MQ=255)                                                                                                                                                            gtgtTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTTTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAActct                                                               <  2:211768/250‑1 (MQ=255)                                                                                                                                                                                gcTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTTTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGGGGTTAAACCCAACACTTCTGCGGCTTTACTGTAGCTg                                          >  2:206195/1‑251 (MQ=255)                                                                                                                                                                                        aTACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTTTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAAcg                                  >  1:89280/1‑251 (MQ=255)                                                                                                                                                                                           cAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTTTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGCATCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACC‑AACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACgcgc                               >  1:241418/1‑250 (MQ=255)                                                                                                                                                                                                     cacGCGCTTCTATGCCCTGCGAAATAGTATCGATTTTGCGTTTGATGGCCTAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGCGCAGCGgtagt                      >  1:105396/1‑250 (MQ=255)                                                                                                                                                                                                          gcTTCTATGCCCTGCGAAATAGTATCGATTTTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGc                                                                                     <  2:162196/182‑1 (MQ=255)                                                                                                                                                                                                          gcTTCTATGCCCTGCGAAATAGTATCGATTTTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGc                                                                                     >  1:162196/1‑182 (MQ=255)                                                                                                                                                                                                             tCTATGCACTGCGAAATAGTATCGATTTTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAACTAACGCCCGGACTCCGTTAACTAAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTTCGGCTTTACTGAAGCTGCCCAAACGCGCAGCGGTAGTAAATGCCa              >  1:162937/1‑250 (MQ=255)                                                                                                                                                                                                                        cGAAATAGTATCGATTTTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTTCTGAAGCTGCCAAAACGCGCAGCGGTAGTAACTGCCAACAAATTCTCtt  >  2:208431/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           ATACCGCCGCCGTCCAGTAGTGTATCGGGTGCGCCGATGGCGATATTGGCCTGATTATTAATAATCGCATCCCAGACGCCGTTATACACTTCGGTGGTGACGGTAATCTGGCAGGTAGGAAACTGCTTTTTCAGCACCTGTAACAAGCGTGCGGTGTGTTTGGGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGCGCAGCGGTAGTAAATGCCAACAGATTCTCTT  >  NC_000913/333949‑334414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑