Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 331,446 C→T H662Y (CAT→TAT)  betT → choline:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913331,4460CT95.2% 61.7 / NA 21H662Y (CAT→TAT) betTcholine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (8/12);  minor base G (0/1);  total (8/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTTGGGTCATCTGGATTTGTTGGTGCATATGGGCGAAGAGCAAAACTTTGTCTATCAGATTTGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTACAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACCGCGCCATGTCTGCATATGGCGCTGTTTCTCCTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTT  >  NC_000913/331203‑331683
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             
cagTTGGGTCATCTGGATTTGTTGGTGCATATGGGCGAAGAGCAAAACTTTGTCTATCAGATTTGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTACAGCAAAGAGCATGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCa                                                                                                                                                                                                                                        <  2:47530/251‑1 (MQ=255)
                    gttgGTGCCTATGGGCGAAGAGCAAAACTTTGGCTATCAGATTTGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCGACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTACAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCATCGTGAAGCGCCGGGCCATa                                                                                                                                                                                                                    <  2:225970/251‑1 (MQ=255)
                    gttgGTGCATATGGGCGAAGAGCAAAACTTTGTCTATCAGATTTGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTACAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCATCGTGAAGCGCCGGGCCAt                                                                                                                                                                                                                     >  1:6728/1‑250 (MQ=255)
                                    gAAGAGCAAAACTTTGTCTATCAACTTTGGCCGCAGCAATCTTCGGTGACGGAATTTACCTACCGCGCCCGCCGCGGTACATCGACCTACTACCGGCTGGAAAACTTCCTGTTAGACGGCCGCCCGGGCAACGACCTGATGGAATACAGCAAGGTGCAGGTGATCACCGATCTTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCCTCGTGAAGCGCCTGGCCATAGCGTGATggtaccgg                                                                                                                                                                                                     >  2:21907/1‑243 (MQ=255)
                                           aaaaCTTTGTCTATCAGATTTGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTACAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGCCGCGTgc                                                                                                                                                                                             >  2:71130/1‑250 (MQ=255)
                                                 ttGTCTATCAGATTTGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTACAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGt                                                                                                                                                                                         <  1:68150/249‑1 (MQ=255)
                                                            aattGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTACAGCAAAGCGCAGGGGATCACCGATATTCTTGACAAGTACGAGCGGCACCTTAACTTTATTTATCTCAATCGTGTAGCGCCGGGCCAAAGCGTGATGTTCCCGAACGCGTGATTGTTATTGCATAAAAc                                                                                                                                                                            >  2:231947/3‑251 (MQ=255)
                                                               tGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTACAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACcg                                                                                                                                                                          <  1:238362/250‑1 (MQ=255)
                                                                        caATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTACAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACCGCGCCATGTca                                                                                                                                                                <  2:178591/251‑2 (MQ=255)
                                                                                      gggCTTTACCTACCGCGCACGCAGAGGTCAATCGACCTACTACCGGCTGGAACCCTTCCTGTTCGAAGGCAGCCAGGGCAACGACCTGATGGACTACAGCAACGAGCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGCCGCGTGCTTGTTATTGCATAAAACCGCGCCATGTCTGCATATGGCGCTGt                                                                                                                                                  >  2:163540/1‑251 (MQ=255)
                                                                                        gCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTACAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACCGCGCCATGTCTGCATATGGCGCTGttt                                                                                                                                                >  1:94617/1‑251 (MQ=255)
                                                                                              cctaccGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTACAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCTTGATTGTTATTGCATAAAACCGCGCCATGTCTGCATAGGGCGCTGTGTCTCCTa                                                                                                                                          <  2:230130/251‑1 (MQ=255)
                                                                                                          gcaGCGGTAAATCGACCTACTACCGGCTGGAAGCCTTCCTGTTAGAAGGCAGCCAGGGCATCGACCTGATGGACTACAGCAAAGTGCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTAGCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGGTCCCGGACGCGTGATTGTTATGGCATAAAACCGCGCCATGTCTGCATATGGCGCTGTTTCTCCTACCTCCTGATACg                                                                                                                              <  2:35728/251‑1 (MQ=255)
                                                                                                                               acCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTACAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCATCGTGAATCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACCGCGCCATGTCTGCATATGGCGCTGTTTCTCCTACCTCTTGATACGTTATATCTATACGGTTAAGcc                                                                                                         <  2:94617/251‑1 (MQ=255)
                                                                                                                                                     aGAAGGCAGCCAGGGCGACGACCTGATGGACTACAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACCGCGCCATGTCTGCATATGGCGCTGTTTCTCCTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATGATTAc                                                                                   >  2:235809/1‑251 (MQ=255)
                                                                                                                                                           cAGCCAGGGCAACGACCTGATGGACTACAGCAAAGAGCAGGTGATCGCCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACCGCGCCATGTCTGCATATGGCGCTGTTTCTCCTACCTCTTGATACGTTATAGCTATACGGTTAAGCCCTTAGTATCTATTGATGATTTCCAGaca                                                                             <  2:28836/251‑1 (MQ=255)
                                                                                                                                                                 gggCAACGACCTGATGGACTACAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACCGCGCCATGTCTGCATATGGCGCTGTTTCTCCTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGAGGATTACCAGACAACAGat                                                                       <  1:71130/251‑1 (MQ=255)
                                                                                                                                                                                            agagCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACCGCGCCATGTCTGCATATGGCGCTGTTTCTcc                                                                                                                                            >  1:171593/1‑155 (MQ=255)
                                                                                                                                                                                            agagCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTTATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACCGCGCCATGTCTGCATATGGCGCTGTTTCTcc                                                                                                                                            <  2:171593/155‑1 (MQ=255)
                                                                                                                                                                                                                     gACCAGGACGAGCGGCACCTTAACTTTATTGATCCCCATCGTGAAGCGCCGGGCCATAGCGGGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACCGCGCCATGTCTGCATATGGCGCTGTTTCTACGACTTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGc                   <  2:185399/251‑1 (MQ=255)
                                                                                                                                                                                                                                      ccTTAACGTTATTTATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACCGCGCCATGTCTGCATATGGCGCTGTTTCTCCTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTGAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGttt  <  1:163540/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             
CAGTTGGGTCATCTGGATTTGTTGGTGCATATGGGCGAAGAGCAAAACTTTGTCTATCAGATTTGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTACAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGAGCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACCGCGCCATGTCTGCATATGGCGCTGTTTCTCCTACCTCTTGATACGTTATATCTATACGGTTAAGCCCTTAGTATCTATTGATGATTACCAGACAACAGATAATAAAAAAGAAAAGAACTATTGCAGCCCAAAACCTACATTTGGGCTGTTGCGAATGTTCAATAAGTTT  >  NC_000913/331203‑331683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: