Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,615,345	C→T	G67S (GGC→AGC)	yjjW ←	putative glycyl‑radical enzyme activating enzyme YjjW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,615,345	0	C	T	100.0%	157.7 / NA	45	G67S (GGC→AGC)	yjjW	putative glycyl‑radical enzyme activating enzyme YjjW
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/19);  total (26/19)

ACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGTTCCAGAATACGAGTGTTGC  >  NC_000913/4615100‑4615585                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 aCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTca                                                                                                                                                                                                                                              >  1:99180/1‑250 (MQ=255)   aGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCCCAGCGTTCCACACCACTTTGCTGTcaac                                                                                                                                                                                                                                            >  1:117116/1‑250 (MQ=255)    ggTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCAACCACAAACGGCAGCTGGGTCGTGGATTCACCGCCACTCACCGTTATCCCTTCGCTAAATAGCAATGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACAACTCTTCAGGCAGGTATCACACTGCTGGAAAACCACAGCGTTCCACACCACTGTGCTGTcaaca                                                                                                                                                                                                                                           >  1:96287/1‑250 (MQ=255)           tGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAACCGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCa                                                                                                                                                                                                                                    >  2:86686/1‑250 (MQ=255)             gcgcAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCCCCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACg                                                                                                                                                                                                                                 <  1:65299/251‑1 (MQ=255)              cgcAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGc                                                                                                                                                                                                                                >  1:218759/1‑251 (MQ=255)                aaGTTGCGGATCTTTTTTGATAGAAGAAAACAGCGCCACCAAAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCt                                                                                                                                                                                                                              <  1:209041/250‑1 (MQ=255)                    tGCGGATCGTTTTTGCTAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACCCCACTTTGCTGTCAACAATCTGCAACGCCTGATg                                                                                                                                                                                                                          >  1:85830/1‑251 (MQ=255)                     gCGGATCTTTTTTGATAGCAGTAAACAGCGCCACAACAACCGCCAGCTGGGTCGTGGTTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGc                                                                                                                                                                                                                         <  1:241813/251‑1 (MQ=255)                        gaccttttttGATAGCAGAAAACAGCCCCACCAAAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGa                                                                                                                                                                                                                      <  2:20117/246‑1 (MQ=255)                               ttagagaGCAGAAAACAGCGCCCCCACAACCGGCAGCTGGGTCGTGGCTTTACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGGGTGGCATGTTGCGGACACCGCTTCAGGCAGGTATCACACTGCGCGCAAACCACAGCGTGCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACtgt                                                                                                                                                                                                                <  1:259107/244‑1 (MQ=255)                                ttgatcacagaacaCAGCGCCACCACAAGCGGCAGCTGGGGCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACtgtg                                                                                                                                                                                                               <  2:58579/237‑1 (MQ=255)                                  gATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGgc                                                                                                                                                                                                             >  2:35986/1‑250 (MQ=255)                                          aaaaCAGCGCCACCACAAACGGCAGCTGGGGCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTcc                                                                                                                                                                                                    <  1:179948/250‑1 (MQ=255)                                                  ccccccaaaaccGGCAGCTGGGTCGTGGTTCCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTc                                                                                                                                                                                            <  2:218759/240‑1 (MQ=255)                                                        acacaCGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCAATGCTTTGCGGACATGGCTAAGCAATTCGTCCACGCTCCTGGATTGCGCCATGGGCGTGGCATGTTGCGGACCCCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGCTGCGGACCCTGTGGCACGCACTCCCCACAGTCATTGc                                                                                                                                                                                       >  2:161495/5‑250 (MQ=255)                                                           aaCGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAACCAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACg                                                                                                                                                                                   >  1:247913/1‑251 (MQ=255)                                                                            ggCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGCCCACGGAt                                                                                                                                                                  >  1:206851/1‑251 (MQ=255)                                                                              cTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCCTGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATtg                                                                                                                                                                >  2:92597/1‑251 (MQ=255)                                                                                     gcctcTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATTGATTGCGCCATGGGCGTGGCATGTTGCGGACCCCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCCCACCCCTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACCCTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTTACAGt                                                                                                                                                         <  1:65948/247‑1 (MQ=255)                                                                                           cACCGTTATCCCTTCGATAACCAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGc                                                                                                                                                   >  2:187247/1‑251 (MQ=255)                                                                                           cACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGc                                                                                                                                                   >  1:102064/1‑251 (MQ=255)                                                                                                                             cGGACATGGATAAGCACTTCGTCCACGCTCATGGCTTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGca                                                                                                                   >  1:79044/1‑248 (MQ=255)                                                                                                                              ggACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGcc                                                                                                                   >  2:32696/1‑248 (MQ=255)                                                                                                                                  atgtcTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACAGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCAATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAACAACAGAGCCAgacgac                                                                                                            >  2:37149/5‑251 (MQ=255)                                                                                                                                     gCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGc                                                                                                         <  1:32696/251‑1 (MQ=255)                                                                                                                                      cTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGTAGCCCTGCAAAAACAGAGCCAGACGACTGcc                                                                                                        >  1:184528/1‑251 (MQ=255)                                                                                                                                                cGTCCACGCTCATGGATTGCGTCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCTCAGTCATTGCAACGTCCCATCGTCCACGGATGGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCggca                                                                                              <  2:230547/251‑4 (MQ=255)                                                                                                                                                     aCGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCa                                                                                         <  1:144233/251‑1 (MQ=255)                                                                                                                                                       gctaaTGGATTGCGCCATGGGAGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCCCACTGCTCGCAAACCACAGCGTTCCCCACCACTTTGCTGTCACCAATCTGCCACGCCTGATGCGGACACTACAGCACGCACTCCCCACAGTCATTGCACAGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTCCAGCCCGGCAAGAACAGAGCCAGTCGACTGCCTGGCCCGTCAACGCAgg                                                                                       >  2:13924/5‑251 (MQ=255)                                                                                                                                                          cATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGgaga                                                                                    <  2:117116/251‑1 (MQ=255)                                                                                                                                                          cATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCACAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAgggg                                                                                     >  2:193928/1‑248 (MQ=255)                                                                                                                                                          cATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTGTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCATCGCAGga                                                                                      <  2:106410/249‑1 (MQ=255)                                                                                                                                                            tGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGgaga                                                                                    >  2:190742/1‑249 (MQ=255)                                                                                                                                                                  gcgcCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATa                                                                            <  1:101036/251‑1 (MQ=255)                                                                                                                                                                                 cATGTTGCGGACACCTCTTCAGGCAGGTATCAAACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGAGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTCTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGTCGACTGCCTGGCCCGTCAACGCAGGAGGCGGGGATACTCTTACTGACTaa                                                              >  2:30573/1‑250 (MQ=255)                                                                                                                                                                                       gCGGTCACCTCTTCAGGCAGGTATCACACTGCGCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGTATAAGGGGATAAGCTTACTGACTAAAGCGCAt                                                       <  1:58037/251‑1 (MQ=255)                                                                                                                                                                                         ggACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATct                                                     >  2:192613/1‑251 (MQ=255)                                                                                                                                                                                             acCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATc                                                                                                                                                                            <  1:239214/128‑1 (MQ=255)                                                                                                                                                                                             acCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTCCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGCTGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATc                                                                                                                                                                            >  2:239214/1‑128 (MQ=255)                                                                                                                                                                                                tctTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTa                                                               >  2:129533/1‑234 (MQ=255)                                                                                                                                                                                                tctTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTa                                                               <  1:129533/234‑1 (MQ=255)                                                                                                                                                                                                            tATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCCGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCAc                                  >  2:96154/1‑251 (MQ=255)                                                                                                                                                                                                                       tCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCACCGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGCTTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAAGAGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGc                       >  2:78180/1‑251 (MQ=255)                                                                                                                                                                                                                                            accacGTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGTTCCAGAATACGAGTGTtgc  <  2:194403/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 ACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGTTCCAGAATACGAGTGTTGC  >  NC_000913/4615100‑4615585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑