Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,580,552	C→T	G304E (GGG→GAG)	hsdS ←	type I restriction enzyme EcoKI specificity subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,580,552	0	C	T	100.0%	68.7 / NA	20	G304E (GGG→GAG)	hsdS	type I restriction enzyme EcoKI specificity subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/12);  total (8/12)

TCGACGCGGCGAACGATTTCGGCTTGTTCTTTTACTGGAGGTAATAAAACAACTTGGGATTTGATATCTTTTCCTGAAATACCTTTTTGACCAGAAGTTGTTTTCACGCAGTTCATCATTGCATTTCGTGCTGAGGGGGATGAAAAAAATATTTCGATATATTCTGGTAAAGCATCTTTGGTTAATCGAGCTCGAATAAGTTTATCAGGATATAGCAAATTTTGATGTTGTAATTTTTTCAATAACCCACAAACACCAACAAATTCTAAACTTCCGTTATAGCGAGTAAATAAAAGATCTCCATCTTGTAATTTGTGGCGGTTTAGTTCACTTTCTGAACATTCTAGAAACCGAATATCGTTTTGATCTACATGGCCAGCACGTACAGAACTAATGCGTAGTATTGGATGACCAACACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTC  >  NC_000913/4580306‑4580791                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                tCGACGCGGCGCACGATTTCGGCTTGTTCTTTTACTGGAGGTACTAAAACAACTTGGGATTTGATATCTTTTCCTGAAATACCTTTTTGACAAGAAGTTGTTTTCACGCAGTTCATCATTGCATTTCGTGCTGAGGGGGATGAAAAAAATATTTCGATATATTCAGGTAAATCATCTTTGGTTACTCGAGCTCGAATAAGTTTACAAGGCTATAGCACATTTTGATGTTGTAATTTTTTCAATAACTcac                                                                                                                                                                                                                                              >  2:20118/1‑250 (MQ=255)                 tttaggCTTGTTCTTTTACTGGAGGTAATAAAACAACTTGGGATTTGATATCTTTTCCTGAAATACCTTTTTGACCAGAAGTTGTTTTCACGCAGTTAATCATTGCATTTCGTGCTGAGGGGGATGAAAAAAATATTTCGATATATTCTGGTAAAGCATCTTTGGTTAATCGAGCTCGAATAAGTTTATCAGGATATAGCAAATTTTGATGTTGTAATTTTTTCAATAACTCACAAACACCAACAAATTCt                                                                                                                                                                                                                             >  2:76843/5‑251 (MQ=255)                                                  caacTTGGGATTTGATATCTTTTCCTGAAATACCTTTTTGACCAGAAGTTGTTTTCACGCAGTTCATCATTGCATTTCGTGCTGAGGGGGATGAAAAAAATATTTCGATATATTCTGGTAAAGCATCTTTGGTTAATCGAGCTCGAATAAGTTTATCAGGATATAGCAAATTTTGATGTTGTAATTTTTTCAATAACTCACAAACACCAACAAATTCTAAACTTCCGTTATAGCGAGTAAATAAAAGAtct                                                                                                                                                                                            <  2:223870/251‑1 (MQ=255)                                                   aacTTGGGATTTGATATCTTTTCCTGAAATACCTTTTTGACCAGAAGTTGTTTTCACGCAGTTCATCATTGCATTTCGTGCTGAGGGGGATGAAAAAAATATTTCGATATATTCTGGTAAAGCATCTTTGGTTAATCGAGCTCGAATAAGTTTATCAGGATATAGCAAATTTTGATGTTGTAATTTTTTCAATAACTCACAAACACCAACAAATTCTAAACTTCCGTTATAGCGAGTAAATAAAAGAtctc                                                                                                                                                                                           <  2:195835/251‑1 (MQ=255)                                                     cTTGGGGTTTGATATCTTTTCCTGAAATACCTTTTTGACCAGCCGTTGTTTTCACGCAGTTCATCATTGCATTTCGTGCTGAGGGGGATGAAAAAAATATTTCGATATATTCTGGTACAGCATCTTTGGTTAATCGAGCTCGCATAAGTTTATCAGGATATAGCAAATTTTGATGTTGTAATTTTTTCAATAACTCACAAACACCAACAAATTCTAAACTTCCGTTATAGAGAGTAATTAAAAGATCTcc                                                                                                                                                                                          >  2:238403/1‑250 (MQ=255)                                                         ggATTTGATATCTTTTCCTGAAATACCTTTTTGACCAGAAGTTGTTTTCACGCAGTTCATCATTGCATTGCGTGCTGAGGGGGATGAAAAAAATATTTCGATATATTCTGGTAAAGCATCTTTGGTGAATCGAGCGCGAATAAGTTTATCATGATATATCAAATTTGGATGTTGGAATTTTTTCAATAACTCACAAACGCCAACAAATTCTAAACTTCCGTTATAGCGAGTAAATAAAAGATCTCCATCtt                                                                                                                                                                                     <  1:259816/251‑1 (MQ=255)                                                         ggATTTGATATCTTTTCCTGAAATACCTTTTTGACCAGAAGTTGTTTACACGCAGTTCATCATTGTATTTCGTGCTGAGGGGGATGAAAAAAATATTTCGATATATTCTGGTAAAGCATCTTTGGTTAATCGAGCTCGAATAAGTTTATCAGGATATAGCAAATTTTTATGTTGTAATTTTTACAATAACTCACAAACACCAACAAATTCTAAACTTCCGTTATAGCGAGTAAAGAAAAGATCTCCATCtt                                                                                                                                                                                     <  2:157971/251‑1 (MQ=255)                                                                 tatCTTTTCCTGAAATACCTTTTTGACCAGACGTTGTTTTCACGCAGTTCATCATTGCCTTTCGTGCTGAGGGGGATGAAAAAAATATTTCGATATATTCTGGTACAGCATCTTTGGTTAATCGAGCTCGAATAAGTTTATCAGGATATAGCAAATTTTGATGTTGTAATTTTTTCAATAACTCACACACACCAACAACTTCTAAACTTCCGTTATAGCGAGTAAATAAAAGATCTCCATCTTGTAATTtg                                                                                                                                                                             >  2:148676/1‑251 (MQ=255)                                                                         ccTGAAATACCTTTTTGACCAGAAGTTGTTTTCACGCAGTTCATCATTGCATTTCGTGCTGAGGGGGATGAAAAAAATATTTCGATATATTCTGGTAAAGCATCTTTGGTTAATCGAGCTCGAATAAGTTTATCAGGATATAGCAAATTTTGATGTTGTAATTTTTTCAATAACTCACAAACACCAACAAATTCTAAACTTCCGTTATAGCGAGTAAATAAAAGATCTCCATCTTGTAAGTTGTGGCGGtt                                                                                                                                                                     <  1:68654/251‑1 (MQ=255)                                                                            gAAATACCTTTTTGACCAGAAGTTGTTTTCACGCAGTTCATCATTGCATTTCGTGCTGAGGGGGATGAAAAAAATATTTCGATATATTCTGGTAAAGCATCTTTGGTTAATCGAGCTCGAATAAGTTTATCATGATATAGCAAATTTTGATGTTGTAATTTTTTCAATAACTCACAAACACCAACAAATTCTAAACTTCCGTTATAGCGAGTAAATAAAAGATCTCCATCTTGTAATTTGTGGCGGTTTAg                                                                                                                                                                  <  2:97566/251‑1 (MQ=255)                                                                                tACCTTTTTGACCAGAAGTTGTTTTCACGCAGTTCATCATTGCATTTCGTGCTGAGGGGGATGAAAAAAATATTTCGATATATTCTGGTAAAGCATCTTTGGTTAATCGAGCTCGAATAAGTTTATCAGGATATAGCAAATTTTGATGTTGTAATTTTTTCAATAACTCACAAACACCAACAAATTCTAAACTTCCGTTATAGCGAGTAAATAAAAGATCTCCATCTTGTAATTTGTGGCGGTTTattttt                                                                                                                                                              >  2:37834/1‑246 (MQ=255)                                                                                   cTTTTTGACCAGAAGTTGTTTTCACGCAGTTCATCATTGCATTTCGTGCTGAGGGGGATGAGAAAACTATTTCGATATATTCTGGTACAGCATCTTTGGTTAATCGAGCTCGAATAAGTTTATCAGGATATAGCAAATTTTGATGTTGTAATTTTTTCAATAACTCACAAACACCAACAAATTCTAAACTTCCGTTATAGCGAGTAAATAAAAGCTCTCCATCTTGTAATTTGTGGCGGtttttgttctat                                                                                                                                                           >  2:36856/1‑242 (MQ=255)                                                                                                  ttgttTTCACGCAGTTCATCATTGCATTTCGTGCTGAGGGGGATGAAAAAAATATTTCGATATATTCTGGTAAAGCATCTTTGGTTAATCGAGCTCGAATAAGTTTATCAGGATATAGCAAATTTTGATGTTGTAATTTTTTCAATAACTCACAAACACCAACAAATTCTAAACTTCCGTTATAGCGAGTAAATAAAAGATCTCCATCTTGTAATTTGTGGCGGTTTTGTTAACttttttcaaatactaac                                                                                                                                            >  2:138105/1‑237 (MQ=255)                                                                                                                                     gAGGGGGATGAAAAAAATATTTCGATATATTCTGGTAAAGCATCTTTGGTTAATCGAGCTCGAATAAGTTTATCAGGATATAGCAAATTTTGATGTTGTAATTTTTTCAATAACTCACAAACACCAACAAATTCTAAACTTCCGTTATAGCGAGTAAATAAAAGATCTCCATCTTGTAATTTGTGGCGGTTTAGTTCACTTTCTGAACATTCTAGAAACCGAATATCGTTTTGATCTACATGTCCAGCACg                                                                                                         <  2:223893/251‑1 (MQ=255)                                                                                                                                                                                                         tttATCAGGATATAGCAAATTTTGATGTTGTAATTTTTTCAATAACTCACAAACACCAACAAATTCTAAACTTCCGTTATAGCGAGTAAATAAAAGATCTCCATCTTGTAATTTGTGGCGGTTTAGTTTACTTTTTGTACAt                                                                                                                                                  >  1:82196/1‑142 (MQ=255)                                                                                                                                                                                                         tttATCAGGATATAGCAAATTTTGATGTTGTAATTTTTTCAATAACTCACAAACACCAACAAATTCTAAACTTCCGTTATAGCGAGTAAATAAAAGATCTCCATCTTGTAATTTGTGGCGGTTTAGTTCACTTTCTGAACAt                                                                                                                                                  <  2:82196/142‑1 (MQ=255)                                                                                                                                                                                                                 gATATAGCAAATTTTGATGTTGTAATTTTTTCAATAACTCACAAACACCAACAAATTCTAAACTTCCGTTATAGCGAGTAAATAAAAGATCTCCATCTTGTAATTTGTGGCGGTTTAGTTCACTTTCTGAACATTCTAGAAACCGAATATCGTTTTGATCTACATGGCCAGCACGTACAGAACTAATGCGTAGTATTGGATGACCAACACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCa                             <  1:31233/251‑1 (MQ=255)                                                                                                                                                                                                                              ttGATGTTGTAATTTTTTCAATAACTCACAAACACCAACAAATTCTAAACGTCCGTTATAGCGAGTAAATAAAAGATCTCCATCTTGTAATTTGTGGCGGTTTAGTTCACTTTCTGAACATTCTAGAAACCGAATATCGTTTTGATCTACATGGCCAGCACGTACAGAACTAAGGCGTAGTATTGGATGACCAACACCACCTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAagatagat                 <  1:238403/250‑1 (MQ=255)                                                                                                                                                                                                                                      gtAATTTTTTCAATAACTCACAAACACCAACAAATTCTAAACTGCCGTTATAGCGAGTAAATAAAAGATCTCCATCTTGTAATTTGTGGCGGTTTAGTTCACTTTCTGAACATTCTAGAAACCGAATATCGTTTTGATCTACATGGCCAGCACGTACAGAACTAATGCGTAGTATTGGATGACCATCACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATGCAAAAtt         <  1:76843/250‑1 (MQ=255)                                                                                                                                                                                                                                             tttCAATAACTCACAAACACCAACAAATTCTAAACTTCCGTTATAGCGAGTAAATAAAAGATCTCCATCTTGTAATTTGTGGCGGTTTAGTTCACTTTCTGAACATTCTAGAAACCGAATATCGTTTTGATCTACATGGCCAGCACGTACAGAACTAATGCGTAGTATTGGATGACCAACACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAActtc  <  1:138105/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                TCGACGCGGCGAACGATTTCGGCTTGTTCTTTTACTGGAGGTAATAAAACAACTTGGGATTTGATATCTTTTCCTGAAATACCTTTTTGACCAGAAGTTGTTTTCACGCAGTTCATCATTGCATTTCGTGCTGAGGGGGATGAAAAAAATATTTCGATATATTCTGGTAAAGCATCTTTGGTTAATCGAGCTCGAATAAGTTTATCAGGATATAGCAAATTTTGATGTTGTAATTTTTTCAATAACCCACAAACACCAACAAATTCTAAACTTCCGTTATAGCGAGTAAATAAAAGATCTCCATCTTGTAATTTGTGGCGGTTTAGTTCACTTTCTGAACATTCTAGAAACCGAATATCGTTTTGATCTACATGGCCAGCACGTACAGAACTAATGCGTAGTATTGGATGACCAACACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTC  >  NC_000913/4580306‑4580791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑