Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,575,319 C→T intergenic (+1388/+1593) yjiT → / ← mcrC putative uncharacterized protein YjiT/Type IV methyl‑directed restriction enzyme EcoKMcrBC subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,575,3190CT90.0% 50.6 / ‑5.0 20intergenic (+1388/+1593)yjiT/mcrCputative uncharacterized protein YjiT/Type IV methyl‑directed restriction enzyme EcoKMcrBC subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (8/10);  minor base G (0/2);  total (8/12)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

GAAGTGTTTAATGAAAACGGCGGCCCCCTTAAGCTTTCTGAAATGTTGTTGTTCCGGGAATTCATGCGTCGCCCTAAACGGACTAACAGCCTGGAAACACAGGGGCTGGTTCAGGTTGGTTACCAGGGGCTGGAGAAAATACATAAGAGCCCCTTGCACTGGCAGGAAAAAGGATTAACGCTGGATGACTGGCGCGATTTTCTCAAGGTTACGTTGGATCATTACGTTCGCGAGAGCAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCA  >  NC_000913/4575083‑4575533
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                      
gAAGTGTTTAATGAAAACGGCGGCCCCCTTAAGCTTTCTGAAATGTTGTTGTTCCGGGAATTCATGCGTCGCCCTAAACGGACTAACAGCCTGGAAACACAGGGGCTGGTTCAGGTTGGTTACCAGGGGCTGGAGAAAATACATAAGAGCCCCTTGCACTGGCAGGAAAAAGGATTAACGCTGGATGACTGGCGCGATTTTCTCAAGGTTACGTTGGATCATTACGTTCGCGAGAGTAACTTCACACAg                                                                                                                                                                                                            <  1:200689/249‑1 (MQ=255)
                           cTTAAGCTTTCTGACAAGTTGCTGTTACGGGAATTCCTGCGTCGCCCTACACGGACTAGCCGCCTGGGCCCACAGGGGCTGGTTCAGGTTGGTTACCAGGGGCTGGAGACCATACATCAGAGCCCCCTGCACTGGCAGGACAAAGGATTACCGCTGGTAGACCGGCGCGATTTTCTCAAGGCTACGTTGGATCATTATGTTCGCGGGAGTAACTTACCACAGCTGGCTGGTGAGCTGAAACACTGGATTgg                                                                                                                                                                               >  2:119870/1‑251 (MQ=255)
                                 cTTTCTGAAATGTTGTTGTTCCGGGAATTCATGCGTCGCCCTAAACGGACTAACAGCCTGGAAACACAGGGGCTGGTTCAGGTTGGTTACCAGGGGCTGGAGAAAATACATAAGAGCCCCTTGCACTGGCAGGAAAAAGGATTAACGCTGGATGACTGGCGCGATTTTCTCAAGGTTACGTTGGATCATTACGTTCGCGAGAGTAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCg                                                                                                                                                                         >  2:147325/1‑251 (MQ=255)
                                                             tCATGCGTCGCCCTAAACGGACTAACAGCCTGGAAACACAGGGGCTGGTTCAGGTTGGTTACCAGGGGCTGGAGAAAATACATAAGAGCCCCTTGCACTGGCAGGAAAAAGGATTAACGCTGGATGACTGGCGCGATTTTCTCAAGGTTACGTTGGATCATTACGTTCGCGAGAGTAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCCAAATTCGTCCGTAACCCg                                                                                                                                             >  1:192359/1‑251 (MQ=255)
                                                             tCATGCGTCGCCCTAAACGGAATAACAGCCTGGAAACACAGGTGCTGGTTCAGGTTGGTTACCAGGGGCTGGAGAAAATACATTAGAGCCCCTTGCACTGGCAGGAAAAAGGATTAACGCTGGATGACTGGCGCGATTTTCTCAAGGTTACGTTGGATCATTACGTGCGCGAGAGGAACTTCACACAGCTGGATGATGAGCTGAAAATCTGGATTGGTAGCCGTTTTGCATCAAAATTCGTCCGTAAccc                                                                                                                                              <  2:222173/250‑1 (MQ=255)
                                                                  cGTCGCCCTAAACGGACTAACAGCCTGGAAACACAGGGGCTGGTTCAGGTTGGTTACCAGGGGCTGGAGAAAATACATAAGAGCCCCTTGCACTGGCAGGAAAAAGGATTAACGCTGGATGACTGGCGCGATTTTCTCAAGGTTACGTTGGATCATTACGTTCGCGAGAGTAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATc                                                                                                                                        <  1:147325/251‑1 (MQ=255)
                                                                              cGGACTAACAGCCTGGAAACACAGGGGCTGGTTCAGGTTGGTTACCCGGGCCTGGAGAAAATACATCAGAGCCACTTGCAATGGCAGGAAAACGGATTAAAGCTGGCTGACTGGCGCGATTTTCTCAAGGTTACGTTGGATCATTACGTTCGAGAGAGTAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAACAGATCCTg                                                                                                                             >  1:120854/1‑250 (MQ=255)
                                                                               ggACTAACAGCCAGTAAACACAGGGGGTGGGTAAGGTGGGTTGCCAGGGGCTGGAGAAAATACATAAGAGCCCCTTGCACTGGCAGGAAAAAGGATTAACGCGGGATGACTGGCGCGATTTTCTCAAGGTGACGTTGGATGATTACGTTCGCGAGAGGAACTTCGCACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGGCTTTCAGCAAAATTCGTCCGTAGCCCGAAATCAAAAGaggctgaa                                                                                                                           <  2:122554/251‑8 (MQ=255)
                                                                                       aGCCGGGAAACACAGGGGCTGGTTCAGGTTGGTTACCAGGGGCTGGAGAAAATACATAAGAGCCCCTTGCACTGGCAGGAAAAAGGATTAACGCTGGATGACTGGCGCGATTTTCTCAAGGTTACGTTGGATCATTACGTTCGCGAGAGTAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCa                                                                                                                   <  1:141599/251‑1 (MQ=255)
                                                                                                        gCTGGGTCAGGTTGGTTACCAGGGGCTGGAGAAAATACATAAGAGCCCCTTGCACTGGCAGGAAAAAGGATTAACGCTGGATGACTGGCGCGATTTTCTCAAGGTTACGTTGGATCAGTACGTTCGCGAGAGTAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACAGACGCTGGCCTc                                                                                                  <  1:178036/251‑1 (MQ=255)
                                                                                                                  gttggttACCAGGGGCTGAAGAAAATACATAAGAACCCCTTGCACTGGCAGGAAAAAGGATTAACGCTGGATGACTGGCGCGATTTTCTCAAGGTTACGTTGGATCATTACGTTCGCGAGAGTAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACAGACGCTGGCCTCAAATTCGTa                                                                                         >  1:71490/1‑250 (MQ=255)
                                                                                                                                   ggAGAAAATACATAAGAGCCCCTTGCACTGGCAGGAAAAAGGATTAACGCTGGATGACTGGCGCGATTTTCTCAAGGTTACGTTGGATCATTACGTTCGCGAGAGTAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGTAATCAAAAGATCCTGAAGATAATCAGAACAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATc                                                                       <  1:123425/251‑1 (MQ=255)
                                                                                                                                          aTACATAAGAACCCCTTGCACTGGCAGGAAAAAGTATTAACGCTGGATGACTGGCGCGATTTTCTCAAGGTTACGTTGGATCATTACGTTCGCGAGAGTAACTTCACACAGCTGGATGATGAGCGGAAAAACTGGATTGGAAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGATTCAAATGATCCTGAAGATAATCAGAAGAGACGGTGGCCTCCAATTCGTAATGGCAACGTATCCCATCGTTTAgg                                                                >  2:25646/1‑250 (MQ=255)
                                                                                                                                                          tgctatGGCAGGAAAAAGGAGTAACGCTGGAGGACTGGCGCGATTTTCGCAAGGTTACGTTTGATCATTACGTTCGCGAGAGTAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAg                                                                                                                                   <  2:115080/163‑1 (MQ=255)
                                                                                                                                                          tGCACTGGCAGGAAAAAGGATTCACGCTGGATGACTGGCGCGATTTTCTCAAGGTTACGTTGGATCATTACGTTCGCGAGAGTAACTTCACACCGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAg                                                                                                                                   >  1:115080/1‑168 (MQ=255)
                                                                                                                                                                ggCAGGAAAAAGGATTAACGCTGGATGACTGGCGCGATTTTCTCAAGGTTACGTTGGATCATTACGTTCGCGAGAGTAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGc                                           <  1:68140/250‑1 (MQ=255)
                                                                                                                                                                                                cgcgATTTTCTCAAGGTTACGTTGGATCATTACGTTCGCGAGAGTAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGa          <  1:36232/251‑1 (MQ=255)
                                                                                                                                                                                                         cacAAGGTTACGTTGGATCATTACGTTCGCGAGAGTAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATTATCAGAACAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAAGTATTGATATTATCa  <  2:123416/248‑1 (MQ=255)
                                                                                                                                                                                                                            cttACGTTCGCGAGAGTAACTTCACACAGCTGGATGATGAGCTGAAAACCTGGATTGGTAGCCGTTTTTCATCACAATTCGTCCGTAACCCGGAATCAAAAGATCCTGCAGATAATCAGAACAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGACGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTc                           >  1:101655/2‑206 (MQ=255)
                                                                                                                                                                                                                            aTTACGTTCGCGAGAGTAACTTCACACAGCTGGATGATGAGCGTAAAAACTGGAGTGGTAGCCGTTTTTCAGCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAAGAGACGCTGGCCTCAAATTCGTAATTGCAACGTAGCCCATCGTTGAGCGACGTTGCTGATGCTGGGGGCGGGATTCaaaagagtc                           <  2:101655/206‑6 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                      
GAAGTGTTTAATGAAAACGGCGGCCCCCTTAAGCTTTCTGAAATGTTGTTGTTCCGGGAATTCATGCGTCGCCCTAAACGGACTAACAGCCTGGAAACACAGGGGCTGGTTCAGGTTGGTTACCAGGGGCTGGAGAAAATACATAAGAGCCCCTTGCACTGGCAGGAAAAAGGATTAACGCTGGATGACTGGCGCGATTTTCTCAAGGTTACGTTGGATCATTACGTTCGCGAGAGCAACTTCACACAGCTGGATGATGAGCTGAAAAACTGGATTGGTAGCCGTTTTTCATCAAAATTCGTCCGTAACCCGGAATCAAAAGATCCTGAAGATAATCAGAACAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCA  >  NC_000913/4575083‑4575533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: