Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,287,464	T→A	intergenic (‑93/‑147)	acs ← / → ytcB	acetyl‑CoA synthetase (AMP‑forming)/protein YtcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,287,464	0	T	A	100.0%	82.2 / NA	24	intergenic (‑93/‑147)	acs/ytcB	acetyl‑CoA synthetase (AMP‑forming)/protein YtcB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (11/13);  total (11/13)

GATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGT  >  NC_000913/4287225‑4287700                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             gATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGt                                                                                                                                                                                                                                   <  1:133884/251‑1 (MQ=255)             aaTTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCaa                                                                                                                                                                                                                       <  1:134818/251‑1 (MQ=255)               tttttCCCTGTTCGCCCCGGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAAt                                                                                                                                                                                                                     >  1:10295/1‑251 (MQ=255)                     ccTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACgg                                                                                                                                                                                                               >  1:220495/1‑251 (MQ=255)                                    aGGTATCAGTTACGGTAATAGAGTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACTGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGATTTTGTTCGCCGTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGa                                                                                                                                                                                                <  1:11456/251‑1 (MQ=255)                                          cAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGGAGAATTGTGCAAATAAACGGCAGGGGAATTTTTGAAGGTc                                                                                                                                                                                           >  2:223400/1‑250 (MQ=255)                                                         aTTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGTGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTTATTTTTGATGGTCAGCAACAAAAGTTGAt                                                                                                                                                                           <  1:188859/251‑1 (MQ=255)                                                                      cGCCTCGCCCTGCTGAGGGTTTATCAGGCAACGGTCTGCGCTGTTGGCCGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATACTATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAAAGGCAGGGAAATTTTTGAAGGTCAGCAACAAAAGTTGATTCATTCTTTGAgg                                                                                                                                                              >  2:166194/1‑251 (MQ=255)                                                                           cGTACTGCTGAGGGTTTATCAGGCAACGGTCGGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACAt                                                                                                                                                         <  1:113471/251‑1 (MQ=255)                                                                                    gAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGTGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAAGTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTat                                                                                                                                                 <  1:174777/250‑1 (MQ=255)                                                                                            aTCAGGCAACGGTCTGAGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCATTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAATAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGATGt                                                                                                                                        >  1:242649/1‑251 (MQ=255)                                                                                                          tGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCATTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATATTTTGAGGAACATGCAGTTATGCATGCTTTTGAAAAAGGGGa                                                                                                                            >  2:42888/1‑249 (MQ=255)                                                                                                                                      tgAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTAtttt                                                                                              >  2:213415/1‑251 (MQ=255)                                                                                                                                                acATGCTTTTGTTCTCCTTGTAGGATGTTAATCATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACtt                                                                                    >  1:177527/2‑251 (MQ=255)                                                                                                                                                    gCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATGTTACATGCACTTACaa                                                                                <  1:213415/251‑1 (MQ=255)                                                                                                                                                                  tGTAGGAGGTTAATAATATTTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTGTTGGTTAGGATGTTGGCGACAGAACACGCAAAAGTAGAATTGTTCAAAGAAACTGCAGGGTAATTTTTTAATGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACAGGCAGTTATGCATGCTTTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTTGTAGTTTACATGCACTTACAATTGATTAAAGaca                                                                   <  1:241363/250‑1 (MQ=255)                                                                                                                                                                     aGGATGTTCATAATATGGGGCATAAGCGATAAATGTAGGGGCATTGGCAGTTTTGTTTAGACTTTGGGCGACATAACGCGAAAAAGGGGAATTGTGCAAATAAACGGCAGGGGAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTCAGCATGCTGTTGAAAAAACGGAAAATACTGACTAACTCTAAAGTGGAATTTTACATGCACTGACAATTGGTTAAAGacaatat                                                               >  2:178470/1‑248 (MQ=255)                                                                                                                                                                             aataatATGGGGCATAAGCGTTAAATGTAGGGTTAGTGGCAGTTTTGTTTAATATTTGGGCGAGAGTACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGTATCCTGCAGTTATGCATTCTGTTGAAAAAGAGGAAGATACCGACTAACTCTAAAGTGGTATTTTACATGCACCTGCAATTGATTAAAGTCAATATTCACAgt                                                        <  2:143841/250‑1 (MQ=255)                                                                                                                                                                                aataTGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTgg                                                    <  2:214281/251‑1 (MQ=255)                                                                                                                                                                                                            ggTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAAt                        >  1:1734/1‑251 (MQ=255)                                                                                                                                                                                                             gTATTGTCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGTTATTTTACATTCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGTCGACCAAta                       <  2:39656/251‑2 (MQ=255)                                                                                                                                                                                                             gTATTGGCAGTTTTGTTTAATAGTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGGTGATTAATTCTTTGAGGAACATGCAGTTAAGCATGCTGTTGAAAAAGAGGAAGATACTTACTAACTCTAAAGTGGTATTTTACATGCACTTTCAAGTGATTAAATACATCATTTACAGTGTGGTTATTTGTTACACAGAGGGGCGAGCAATg                       <  2:224286/251‑1 (MQ=255)                                                                                                                                                                                                                                 tATTTGGGCGACAGAACACGCAAAGGTAGACTTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGCCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACGGATTAACTCTAAAGGGGTATTTTACAGGAACTTAAAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGGGCAATGCAATGACATTGTAGTTGCgg   >  2:49406/1‑251 (MQ=255)                                                                                                                                                                                                                                  aTTTGGGAGAAAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACATCATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGGCAGTGTAGGTGCGGt  <  2:1854/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             GATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGT  >  NC_000913/4287225‑4287700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑