Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,180,421	C→T	T142T (ACC→ACT)	rplJ →	50S ribosomal subunit protein L10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,180,421	0	C	T	100.0%	72.6 / NA	26	T142T (ACC→ACT)	rplJ	50S ribosomal subunit protein L10
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/13);  total (13/13)

AAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTATGTCTGTAATGGACGTTGTAGAACTGATCTCTGCAATGG  >  NC_000913/4180189‑4180641                                                                                                                                                                                                                                         |                                                                                                                                                                                                                             aaGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGTGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGAAGCTTCGGCt                                                                                                                                                                                                            <  2:88212/251‑1 (MQ=255)           gTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGAAGCTTCGGCTGGCAAACTgg                                                                                                                                                                                                  >  2:205158/1‑251 (MQ=255)             tCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGAAGCTTCGGCTGGCAACCTGGtt                                                                                                                                                                                                >  2:192220/1‑251 (MQ=255)                                  ttgttgGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTAcg                                                                                                                                                                            <  1:31444/250‑1 (MQ=255)                                        gTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCg                                                                                                                                                                     >  1:88402/1‑251 (MQ=255)                                          ccGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGa                                                                                                                                                                    <  1:33170/250‑1 (MQ=255)                                           cGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGaa                                                                                                                                                                   >  1:224614/1‑250 (MQ=255)                                           cGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGaa                                                                                                                                                                   >  1:63607/1‑250 (MQ=255)                                           cGACCCTGATTGCATACGCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGTCAACTATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGaaa                                                                                                                                                                  <  2:36664/251‑1 (MQ=255)                                                      gCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGCAGCTGCtt                                                                                                                                                        >  1:1813/1‑250 (MQ=255)                                                                   aaCACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGAAGCTTCGGc                                                                                                                                                                                                             >  1:19109/1‑184 (MQ=255)                                                                   aaCACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGAAGCTTCGGc                                                                                                                                                                                                             <  2:19109/184‑1 (MQ=255)                                                                     cacCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCttt                                                                                                                                        >  1:81467/1‑251 (MQ=255)                                                                         cggtcgcTGCTGCTCTTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGAACGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACTCCTGATTGCAACTATGAAAGAAGCTTCGGCTGGCGAACTGGTTCGTACTCTGGCTGCGGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTaa                                                                                                                                    <  1:20916/247‑1 (MQ=255)                                                                                       tgtctgtcctaagggtgcacgAAGGCGAATGTAAAATTTGATGTCAAAGCCGCTGCCTTGGAAGGTGAGCTGATCCCGGCGTCCCATATCGACCGCCTGGCAAATCTGCCGACCTACGAAGAAGCAATTTCACGCCTGAGGGCAAATATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTCCTCTGGCTGCTGTACGCGATGCGAAAGACGCTGCGTAACCGAAGTTATCTTTTTAACGCATTCgttcac                                                                                                                       <  2:231990/231‑6 (MQ=255)                                                                                                              agtgAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGACCCCGGCGTCTCAGATCGACCGCCTTGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTTATGGCAACTATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGTTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATGCTGATATTCAg                                                                                               <  2:219429/248‑1 (MQ=255)                                                                                                                 gAATGCGAAATTAGAGGTCAGAGCCGCACCCATTGAAGGAGAGCTGAGCCCGGCGTCTCAGAACGACCGCCTGGCCATTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGCTGGCAACTATGATAGAAGCTTCGGCTGGCAACCTGGTCCGTACTCTGGATGCTGACCGCAATGAGACAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATGAGCTACCGTATAAACTTATTCTGATATTCAggc                                                                                             >  2:219605/1‑249 (MQ=255)                                                                                                                 gAATGCAACATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACTATGCAAGAAGCTTCGGCTGGCAAACTGGCTCGTACTCTGGCTGCTGTACGCGATGCGACAGAAGCTGCTTAATCGACGTTATCTTTTTAAAGCATTCGCTCACGTATACACTTATTCTGATATTCAGGa                                                                                             >  1:137065/1‑250 (MQ=255)                                                                                                                                         cgcggccgttGAAGGTGAGCTGATACCGGCGTCTCAGATCGACCGCCTGGCAACTCTGACGACCTACGAAGAAGCAAGTGCACGCCTGATGGCAACTATGAAAGAAGCTTAGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGATTAAACGCAGGTATCTTGTTAACGCATTCGCTTACGTATAAACTTATGCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAg                                                                    <  2:114656/243‑1 (MQ=255)                                                                                                                                             gccgttGAAGGTGAGCTGATCCCGGCGTCGCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAGTGTCTATCACTAAAGATCa                                                                <  2:149507/247‑1 (MQ=255)                                                                                                                                                                           cAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGTACGCCTGATGGCAACTATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCGGTACGCGATGTGAAAGAAGCTGCTTAATCGCAGTTATCTGTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAAGTTAAATGTCTATCACTAAAGATCAAATCATGGAAGCAGTTGCAGCTATGTCTGt                                  <  2:164343/251‑1 (MQ=255)                                                                                                                                                                                 gACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGACGCTTCGGCAGGCAAACTGGTTCGTACTCTGGCTGCTGTACACGATGCGAAAGAAGATGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGCTGCAGCTATGTCTGTAATgg                             >  1:238851/1‑250 (MQ=255)                                                                                                                                                                                                      aCCTACGAAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTATGTCTGTAATGGACGTTGTAGAACTGATCTCTGc       <  1:48033/251‑1 (MQ=255)                                                                                                                                                                                                           cGAAGAAGCAATTGCACGCCTGATGGCAACTATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTATGTCTGTAATGGACGTTGTAGAACTGATCTCTGCAATgg  >  1:192583/1‑251 (MQ=255)                                                                                                                                                                                                                                     cAACTATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTTCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCATTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACACTTTaaa                                                                                   >  2:11967/1‑144 (MQ=255)                                                                                                                                                                                                                                     cAACTATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGTGTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTaaa                                                                                   <  1:11967/144‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                             AAGGTACTCCGTTCGAGTGCCTGAAAGACGCGTTTGTTGGTCCGACCCTGATTGCATACTCTATGGAACACCCGGGCGCTGCTGCTCGTCTGTTCAAAGAGTTCGCGAAAGCGAATGCAAAATTTGAGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTATGTCTGTAATGGACGTTGTAGAACTGATCTCTGCAATGG  >  NC_000913/4180189‑4180641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑