Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,109,671	C→T	R280C (CGT→TGT)	sbp →	sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,109,671	0	C	T	100.0%	89.8 / NA	28	R280C (CGT→TGT)	sbp	sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/13);  total (15/13)

TTGTCGAGCGCGGAATTGGCGATGTACTGATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACCGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCGATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCATCTCTTTGAGG  >  NC_000913/4109429‑4109914                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    ttGTCGAGCGCGGAATTGGCGATGTACTGATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTTCTGTCcgcg                                                                                                                                                                                                                                              <  2:157668/250‑1 (MQ=255)      gAGCGCGGAATTGGCGATGTACTGATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGc                                                                                                                                                                                                                                        >  1:195177/1‑251 (MQ=255)             gAATTGGCGATGTACTGATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGATTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTg                                                                                                                                                                                                                                 <  2:96710/251‑1 (MQ=255)               aTTGGCGATGTACTGATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGGGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGc                                                                                                                                                                                                                               >  1:129934/1‑251 (MQ=255)                  ggCGATGTACTGATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGCAAGATAAATTCGAAATCGTCCCGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGCAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGa                                                                                                                                                                                                                             >  2:244101/1‑250 (MQ=255)                       tGTACTGATTGCCTGGGAAAAAGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGt                                                                                                                                                                                                                       >  2:223876/1‑251 (MQ=255)                        gTACTGAATGCCTGGGAAAACGAAGCTCTGCCGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGGTGAGGTGGCGAAAAAGTa                                                                                                                                                                                                                      <  2:47605/251‑1 (MQ=255)                         tACTGATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTAc                                                                                                                                                                                                                     >  2:185816/1‑251 (MQ=255)                            tGATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAACGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGACAAACTACTACTGTCCGCGCGACGCTGGGGTGGCGAAAAAGTACGa                                                                                                                                                                                                                   >  2:2600/1‑250 (MQ=255)                                  ccTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCg                                                                                                                                                                                                            >  1:179143/1‑251 (MQ=255)                                               aGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGa                                                                                                                                                                                               <  1:37330/251‑1 (MQ=255)                                                   ctgctgGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGtt                                                                                                                                                                                           >  1:21001/1‑251 (MQ=255)                                                   ctgctgGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGAGGAAGCCTACCTGAAATATCTCTACTCGCCAGCAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTAAGAAAATGCGTTTCCAAAGCTGAAGtt                                                                                                                                                                                           >  1:259165/1‑251 (MQ=255)                                                                aTGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATtgat                                                                                                                                                                              >  1:29509/1‑251 (MQ=255)                                                                  gAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATtgatg                                                                                                                                                                             <  2:21001/250‑1 (MQ=255)                                                                    aCTGGGGAAAGATAAATTCGACATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATtgatga                                                                                                                                                                            >  2:133522/1‑249 (MQ=255)                                                                    aCTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGAGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATtgatga                                                                                                                                                                            >  1:201272/1‑249 (MQ=255)                                                                           aaaGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTcgg                                                                                                                                                                   <  1:170420/251‑1 (MQ=255)                                                                                                         gagtCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCAtt                                                                                                                                      <  1:190672/250‑1 (MQ=255)                                                                                                                   tCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGACAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAAcggc                                                                                                                           >  1:23996/1‑251 (MQ=255)                                                                                                                    cGCAGAGCCAACCGTGTCGGTGGGCGATAAAGTGGTCGAGAAAAAAGGCACTAAAGAGGAGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAAcggcg                                                                                                                          >  2:198055/1‑251 (MQ=255)                                                                                                                                   gtCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCgatcaga                                                                                                           <  1:133522/251‑1 (MQ=255)                                                                                                                                                              aaaaGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCGATCAGATCAGCAAACGCTGATTTCCCCAGGATa                                                                                <  2:201272/251‑1 (MQ=255)                                                                                                                                                                  ggTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCTGTACGTTCGATCATAGCAGCAAACGCTGATTTCCCCAGGATAATTa                                                                            <  2:24193/251‑1 (MQ=255)                                                                                                                                                                                           aCCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAAGTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCGATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGAc                                                    <  1:201496/250‑1 (MQ=255)                                                                                                                                                                                                                 aGAAGGTCAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCGATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGAtt                             <  1:223876/251‑1 (MQ=255)                                                                                                                                                                                                                        cAGGAAATTGCCGCGAAAAACTACTACTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCGATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGt                       <  1:17192/250‑1 (MQ=255)                                                                                                                                                                                                                                            ctactaCTGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGAACGTTCGATCAGAACAACAAACGCTGATTTTCCCAGGGTAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCCCGGTTTTGCGTTACCCTTGCATCTCTTTGAgg  >  1:157475/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    TTGTCGAGCGCGGAATTGGCGATGTACTGATTGCCTGGGAAAACGAAGCTCTGCTGGCAGCGAATGAACTGGGGAAAGATAAATTCGAAATCGTCACGCCGAGTGAGTCTATCCTCGCAGAGCCAACCGTGTCGGTGGTCGATAAAGTGGTCGAGAAAAAAGGTACTAAAGAGGTGGCGGAAGCCTACCTGAAATATCTCTACTCGCCAGAAGGTCAGGAAATTGCCGCGAAAAACTACTACCGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCGATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCATCTCTTTGAGG  >  NC_000913/4109429‑4109914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑