Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,074,275	C→T	A179A (GCC→GCT)	yihV →	6‑deoxy‑6‑sulfofructose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,074,275	0	C	T	100.0%	113.4 / NA	38	A179A (GCC→GCT)	yihV	6‑deoxy‑6‑sulfofructose kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/24);  total (14/24)

ACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGACTGGCTGGAAAATGGTGGGCGTCAGCATCAACCGGCCTTCAAAGTTGATGTGGTAGATACCACAGGTGCGGGTGATGTTTTTCACGGCGCTTTGGCGGTGG  >  NC_000913/4074039‑4074495                                                                                                                                                                                                                                             |                                                                                                                                                                                                                             aCCAAAGGTGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCGGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGGGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGTGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCggg                                                                                                                                                                                                                <  2:93424/251‑1 (MQ=255)    aaaGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGAGTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGTCGTCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTTAGCGGGTGTCAGTAAGCGATCACGCGGCTTTTTCAGAGCCTGGTCt                                                                                                                                                                                                             <  2:124861/251‑1 (MQ=255)        gCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGgcg                                                                                                                                                                                                         <  2:221357/251‑1 (MQ=255)                   aTCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGGGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACggg                                                                                                                                                                                              >  1:145732/1‑251 (MQ=255)                        tAACTTCCCCAGCCCGGATCTGTCGCGTGACGCAGAGTGGTTGGAGGAAAGTGATTTCTCGCAGTGGGATGTGGTGCTGGCAGATGGACGCTGGCTCGACGGGGCTAAAAAAGCCTTCCCCCTGGCCCGTCAGGCGGGGGTGATGACGGTTGTGGTCGGGGATAGTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTGGTTCATAACCGGGCCTGTCGGGCTTAAcgtgcgtg                                                                                                                                                                                          <  2:114487/250‑1 (MQ=255)                             aCCCCCGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAag                                                                                                                                                                                      >  1:214049/1‑249 (MQ=255)                             aCCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGacag                                                                                                                                                                                      >  1:214508/1‑246 (MQ=255)                                       ggATCTGCTGCTTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATTTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCGGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATAGCAGTGAGCTGGTGGCATGAAGCGGTCACGCGGCTTGTTCAGAACCGGGTCTGGCGCGCGTAACGGGGGTTAAAGAGATGGCCAGTg                                                                                                                                                                          <  2:25397/251‑1 (MQ=255)                                       ggATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTg                                                                                                                                                                          >  1:114866/1‑251 (MQ=255)                                                     aCGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGCGTGATGACCGTTCTGGACGGGGACATCACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGGGATAGCCAGTGCGCTaaacaaggc                                                                                                                                                             >  2:68509/1‑244 (MQ=255)                                                          gagTGGTTGGAGGAAATTGATTTCTCTCAGTGGTATGTTGGGCGGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGTCATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAATCGAGCTCGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAAc                                                                                                                                                       <  1:116393/251‑1 (MQ=255)                                                          gagTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAAc                                                                                                                                                       >  1:42269/1‑251 (MQ=255)                                                                   gaggaAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACaa                                                                                                                                               >  2:133108/1‑250 (MQ=255)                                                                                   ctcAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTAtgtg                                                                                                                              >  1:226867/1‑251 (MQ=255)                                                                                          ggATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAgg                                                                                                                       <  1:68509/251‑1 (MQ=255)                                                                                                   catggcatgtGTACGCTGGCTCGACGGCTCTTAAAAATCCTTCACCCTGGCCCGTGAGGCGGGTGTGGTGACCGTTCGGGACGTGGACATTACGCCGCAGGATATCAGTGGGCTGGGGGCATGAAGCGATCACGCGTCTTTTGCAGAACCGGGTCTGGCTCGCTTAACGGGCGTGAAAGAGATTGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCac                                                                                                              <  2:147033/242‑2 (MQ=255)                                                                                                      ggCAGATGTACGCTGGCACGTCGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGc                                                                                                            <  1:258520/250‑1 (MQ=255)                                                                                                      ggCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCGCCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGGTCACAAATGGTCATGTCTATGGGACCCGGGGTAGCGCAGGCt                                                                                                           <  2:101510/251‑1 (MQ=255)                                                                                                       gCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCCCCCTGGCCCGTCAGGAGGGTGTGCTGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCCGTGAGCTGGTGGCATTCAGCGATCACGCGGCTTTTTCAGAACCGGCGCTGGCGCGCTTAACAGGCGTGACAGCGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGTTCATGTTTATGTGACCCAGGGTAGCGCAGGCTg                                                                                                          >  2:94985/1‑251 (MQ=255)                                                                                                             gTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGActg                                                                                                    <  2:15550/251‑1 (MQ=255)                                                                                                                 gCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGActggctg                                                                                                <  1:632/251‑1 (MQ=255)                                                                                                                         gacgGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGACTGGCTGGAAAAtgg                                                                                        >  1:137206/1‑251 (MQ=255)                                                                                                                           cgGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTTCGACTGGCTGGAAAAtggtg                                                                                      <  2:63036/251‑1 (MQ=255)                                                                                                                           cgGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATGACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTGCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTTTATGTGACCCAGGGTAGCGCAGGCTGCGACTGGCTGGAAAAtggtg                                                                                      <  2:250918/251‑1 (MQ=255)                                                                                                                              cgcTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGACTGGCTGGAAAATGGTGGGc                                                                                   <  2:226867/251‑1 (MQ=255)                                                                                                                                   aaaaaGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGACTGGCTGGAAAATGGTGGGCGTc                                                                                >  1:101373/1‑249 (MQ=255)                                                                                                                                       aGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGACTGGCTGGAAAATGGTGGGCGTCAGCATc                                                                          <  2:187807/251‑1 (MQ=255)                                                                                                                                                        ggcAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGACTGGCTGGAAAATGGTGGGCGTCAGCATCAACCGGCCTTCAAAGtt                                                         <  2:137206/249‑1 (MQ=255)                                                                                                                                                           aGGCGGGTGTGATGCCCGTTCTGGACGGGGACATCACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGCTCACGCGGCTTGTTCAGAACCGGGTCTGGCGCGCGTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCGCAAAGGGTCATGTCTATGTGACCCAGGGTAGCTCAGGCTGCGACTGGCTGGAAAATGGTGGGCGTCAGCACCGTCCGGCCTTCAAATTTGAt                                                      <  2:74318/251‑1 (MQ=255)                                                                                                                                                                ggTGTGATGACCGTTCTGGACTGGTACATTACGCCTCAGGATATCAGTGAGCTGGTGGCAATAATCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGACTGGCTGGAAAATGGTGGGCGTCAGCATCAACCGGCCTTCAAAGTTGATGTGGt                                                 <  2:114866/251‑1 (MQ=255)                                                                                                                                                                  tgtgATGACCGTTCTGGACGGGGACATTACGCAGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAAAGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGACTGGATGGACAATGGTGGGCGTCAGCATCAACCGGCCTTCAAAGTTGATGTGGTa                                                >  2:162407/1‑250 (MQ=255)                                                                                                                                                                                 ggACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGACTGGCTGGAAAATGGTGGGc                                                                                   <  2:142449/200‑1 (MQ=255)                                                                                                                                                                                 ggACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGACTGGCTGGAAAATGGTGGGc                                                                                   >  1:142449/1‑200 (MQ=255)                                                                                                                                                                                 ggACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGCCTATGTGACCCAGGGTAGCGCAGGCTGCGACTGGCTGGAAAATGGTGGGCGTCAGCATCAACCGGCCTTCAAAGTTGATGTGGTAGATACCACAGGTGc                                  <  1:133108/249‑1 (MQ=255)                                                                                                                                                                                  gACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGACTGGCTGGAAAATGGTGGGCGTCAGCATCAACCGGCCTTCAAAGTTGATGTGGTAGATACCACAGGTGCggg                               >  2:188268/1‑251 (MQ=255)                                                                                                                                                                                              aCGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGACTGGCTGGAAAATGGTGGGCGTCAGCATCAACCGGCCTTCAAAGTTGATGTGGTAGATACCACAGGTGCGGGTGATGTTTTTCa                   <  1:109702/251‑1 (MQ=255)                                                                                                                                                                                                          atCAGTGAGCTGTTGGCATTAAGCGATCACGCGGCTTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGACGGGCTGGAAAATGGTGGGCGTCAGCATCAACCGGCCTTCAAAGTTGATTTGGTAGATACCACAGGTGCGGGTGATGTTTTTCACGGCGCTTTGGc       <  1:162407/251‑1 (MQ=255)                                                                                                                                                                                                                gAGCTGGTGGCATTAAGCGATCACGCGGCTTTGTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGACTGGCTGGAAAATGGTTGGCGTCAGCATCAACCGGCCTTCAAAGTTGATGTGGTAGATACCACAGGTGCGGGTGATGTTTTTCACGGCGCTTTGGCGGTgg  <  1:177578/250‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                             ACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTGGACGGGGACATTACGCCGCAGGATATCAGTGAGCTGGTGGCATTAAGCGATCACGCGGCCTTTTCAGAACCGGGTCTGGCGCGCTTAACGGGCGTGAAAGAGATGGCCAGTGCGCTAAAACAGGCACAAACGCTCACAAATGGTCATGTCTATGTGACCCAGGGTAGCGCAGGCTGCGACTGGCTGGAAAATGGTGGGCGTCAGCATCAACCGGCCTTCAAAGTTGATGTGGTAGATACCACAGGTGCGGGTGATGTTTTTCACGGCGCTTTGGCGGTGG  >  NC_000913/4074039‑4074495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑