Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,024,199	C→T	P216S (CCA→TCA)	tatD →	3' ‑> 5' ssDNA/RNA exonuclease TatD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,024,199	0	C	T	100.0%	133.5 / NA	41	P216S (CCA→TCA)	tatD	3' ‑> 5' ssDNA/RNA exonuclease TatD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/20);  total (21/20)

GCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGCCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGC  >  NC_000913/4023952‑4024440                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  gCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTcat                                                                                                                                                                                                                                                <  1:153037/251‑1 (MQ=255)  cGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTAGCTGCTCCCTCGCGATCTCACGCCAAAGTcatc                                                                                                                                                                                                                                               <  1:199406/251‑1 (MQ=255)      ttATGTCATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCatcatc                                                                                                                                                                                                                                            <  2:230517/250‑1 (MQ=255)                                 aTAAACTGCCGGGTGCGGTTCGTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGGTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGccc                                                                                                                                                                                                                <  2:31990/251‑1 (MQ=255)                                               gCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCCGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGCTCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACg                                                                                                                                                                                                  >  2:153978/1‑251 (MQ=255)                                                               ttACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTTCAACGTATTGCGCACTGGc                                                                                                                                                                                    <  1:186125/249‑1 (MQ=255)                                                                 aCCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTg                                                                                                                                                                                 <  1:106647/250‑1 (MQ=255)                                                                    ggCACACGCGAAGGGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCCTTACCGGTTGGGATTGCGCTGAACGAGGCGGCCTGGAGATGCGGGAACTTTTGCAGTTGATTCCGGCGGAAAAATTACTGATCGAAAATGCTGCGCCGCATATGCTCCCTCGCGATCTCACGCCACAGTCATCATACCGGCGCAACGAGCAAGCCCATCTGCCCCCTATTTTGCCACGTATTGCGCACTGGCGTggcg                                                                                                                                                                              >  2:238909/1‑248 (MQ=255)                                                                    ggCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGCACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTgcg                                                                                                                                                                                           >  1:115380/1‑237 (MQ=255)                                                                    ggCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAATTTATCATCCCTGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTgcg                                                                                                                                                                                           <  2:115380/237‑1 (MQ=255)                                                                         acGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCTGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATg                                                                                                                                                                        >  1:21542/1‑251 (MQ=255)                                                                             gAAGAGATGCCGGCATGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGCTGCACGAAGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCCCGCCAAAGTCATCATCCCGGCCCAACGAGCCAGCCCATCTGCCCCATATTTTGCACCGTa                                                                                                                                                                                                >  2:126194/1‑223 (MQ=255)                                                                             gAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGGGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCAGCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTa                                                                                                                                                                                                <  1:126194/223‑1 (MQ=255)                                                                                      cAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGGGGAGAAGCTGCCGCAtggctggc                                                                                                                                                           >  2:148694/1‑251 (MQ=255)                                                                                       aGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATggctggct                                                                                                                                                          >  1:64562/1‑251 (MQ=255)                                                                                         gcgtgcgtGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGc                                                                                                                                                        <  1:258765/251‑1 (MQ=255)                                                                                                        ggAATTTATATCGGCATTACCGGTGGGGTTGGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCTCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCTAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTa                                                                                                                                          <  1:238308/250‑1 (MQ=255)                                                                                                         gAATTTATATCGGCAGTTCCGGGTGGGTTTGCGATGAACGACGCGGACTGTAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGTAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAAt                                                                                                                                        <  2:163781/251‑1 (MQ=255)                                                                                                               atatCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCaa                                                                                                                                   >  2:71838/1‑250 (MQ=255)                                                                                                                tatCGGCATTACCGGATGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGGTTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGCGCCAGCACATCTGACCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGCAGATGCCGCATGGCTGGTTGCCACCACGGATGATAATGTCaaa                                                                                                                                  >  2:186685/1‑250 (MQ=255)                                                                                                                   cGGCATTAGCGGTTGGGTGTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCGAATGTCAAAacac                                                                                                                              <  1:208927/251‑1 (MQ=255)                                                                                                                       aTTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGtt                                                                                                                          <  1:240994/251‑1 (MQ=255)                                                                                                                         tACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAAACCTGTTTg                                                                                                                        >  1:10620/1‑251 (MQ=255)                                                                                                                                tGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGc                                                                                                                 >  2:83362/1‑251 (MQ=255)                                                                                                                                       gCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTa                                                                                                           <  1:167570/250‑1 (MQ=255)                                                                                                                                              acgacgCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGCTCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGc                                                                                                   >  1:160145/1‑251 (MQ=255)                                                                                                                                                    cgGAATGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCCTCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGCTTGCGTTTTAGAGTTTGCGGAACt                                                                                             >  2:66730/1‑251 (MQ=255)                                                                                                                                                     ggACTGGAGCTGCGCGAATTTTTGCATGTGATACCGGCGGAAAACTTACTGATCGGAACTGATGCGCCGGATCTACTCCCCAGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGATCCAGCCAATCTGCCCCAACTTTTGCAACGTATTGCGCACTGTCGCGGGGACGATGCCGCATGCCTCGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTc                                                                                            >  2:116345/1‑251 (MQ=255)                                                                                                                                                                   ggAACTTTTGCCGATGATTCCGCCGGAAATATTACTGATCGAAACTGCTGCGCCGTATCTGCTCCCTCGCGATTTCACGACAAAGTCATCATCCAGGCCCAACGAGCCAGCCCATCCGCCCAATCTTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGACGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTCGAGTTTGCTGTACTAGGAATTCTTCacac                                                                              >  2:105786/1‑251 (MQ=255)                                                                                                                                                                      aCTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGTCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTGTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACtgt                                                                           <  1:132969/251‑1 (MQ=255)                                                                                                                                                                           tGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCtt                                                                       <  2:64562/250‑1 (MQ=255)                                                                                                                                                                            gCCGTTGATTCCGGCGGCAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTa                                                                      >  2:176435/1‑250 (MQ=255)                                                                                                                                                                                                       gATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAATCACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAAt                                           <  2:95041/250‑1 (MQ=255)                                                                                                                                                                                                                tgatgctgcaaatcctcccacTCGCGATCTCACCCCAAACTCATAAGCTCGGCGCAACGAGCCAGCACACTTGCCCCACATTTAGAAACGTATTGCGAACTGCAGTGGAGCAGATGCCACATGGCCGGCTGCCAACACCGATACTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGGGTTTGCGGAAATCGGTATTCTTCACACTGTGCTTAATATATTTATTAATAAGATTAAGCAATTGAATGgatc                                 >  2:232892/21‑249 (MQ=255)                                                                                                                                                                                                                         gTATCTGCTCCCTCGCGATCTCACGCCACAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGAGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATACGATTAAGCAATCGCATGGAGCGAGCCTCAc                        >  2:26479/1‑251 (MQ=255)                                                                                                                                                                                                                               gCTCCCTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACGGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATc                    <  2:21542/249‑1 (MQ=255)                                                                                                                                                                                                                                  cccTCGCGATCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCCGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGtt               >  2:135279/1‑251 (MQ=255)                                                                                                                                                                                                                                          aTCTCACGCCAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTACTCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGaa        >  2:116598/1‑250 (MQ=255)                                                                                                                                                                                                                                               aCGCCAAAGTCATCATCCCGGCGCTACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCTCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACGGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGc  <  1:94774/251‑1 (MQ=255)                                                                                                                                                                                                                                                  ccAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTtta                                                             <  1:102294/189‑1 (MQ=255)                                                                                                                                                                                                                                                  ccAAAGTCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTtta                                                             >  2:102294/1‑189 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  GCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATCTCACGCCAAAGCCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGC  >  NC_000913/4023952‑4024440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑