Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	284,535	T→G	A445A (GCT→GCG)	yagF →	D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	284,535	0	T	G	100.0%	154.8 / NA	55	A445A (GCT→GCG)	yagF	D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (23/32);  total (23/32)

CGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCA  >  NC_000913/284292‑284776                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  cgTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGa                                                                                                                                                                                                                                              >  1:189631/1‑249 (MQ=255)  gTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGCCGGGCAACATCGCGCCGGAAgg                                                                                                                                                                                                                                           >  1:11135/1‑251 (MQ=255)  gTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAgg                                                                                                                                                                                                                                           <  1:219218/251‑1 (MQ=255)       ggAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCgg                                                                                                                                                                                                                                      <  1:34443/251‑1 (MQ=255)         aGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTg                                                                                                                                                                                                                                    >  2:15447/1‑251 (MQ=255)                       cTGCGCGACCTCGGCCTGCTGCATCTGGCCGACATGAACGTGACCGGCCCGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGGGCCTGAGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGGGAAGGCCAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGGGCACAAGGCCACGGc                                                                                                                                                                                                                      >  2:35974/1‑251 (MQ=255)                         gcgcGGCCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGc                                                                                                                                                                                                                      <  2:63180/249‑1 (MQ=255)                                    gCCTGCTGCATCTGGACGCAATGACCGTGACCGGCCAGACGGCGGGCGCGAACCTTGAATGGTGACAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGCGATCAAGGCCACGGCAGTCGACCCGTCg                                                                                                                                                                                                         >  2:162803/1‑251 (MQ=255)                                     ccTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGGGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGCCGGTCTGCTTCCCGACGGGAAACATCGCGCCGGAAGGATCGGTGCTCAAGGCCACGGCGAACGACCCGTCgg                                                                                                                                                                                                        >  2:33555/1‑251 (MQ=255)                                        gcggcagcTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCggt                                                                                                                                                                                                       <  1:143440/242‑1 (MQ=255)                                                 ggACGCCATGACCGTGCCCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAg                                                                                                                                                                                            <  1:222578/251‑1 (MQ=255)                                                      ccATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGc                                                                                                                                                                                       <  1:102052/251‑1 (MQ=255)                                                                gaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGGGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATaccac                                                                                                                                                                              <  1:156762/250‑1 (MQ=255)                                                                gaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGCGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATaccacc                                                                                                                                                                             >  1:173126/1‑251 (MQ=255)                                                                   cgGCCAGACGGGGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACcaca                                                                                                                                                                          <  1:110232/251‑1 (MQ=255)                                                                         gACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGCCGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCACGGCCACGGCGATCGCCCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACAccggcc                                                                                                                                                                    >  2:102945/1‑251 (MQ=255)                                                                                    agaACCTTGACTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggtgcgggtg                                                                                                                                                         >  2:36564/1‑251 (MQ=255)                                                                                     gaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCCCACCGGCCGGGTGCGGgtgt                                                                                                                                                        >  1:158936/1‑251 (MQ=255)                                                                                           tGAATGGTGGCAGGCGTCCGAGCGCCGGGAGCGCTTCCGCCCGTGCCTGCGCGAGCAGGACGGCGTCGAGCAGGACGACGTGAACCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTCACCTCGAAGGTCTGCTTCCCGCCGGGCAACATCGCGCCGGAAGGGTCGGTGATCACGGCCACGGCGATCGACCCGTCGGCGGAGGGCGACGCAGGCGTAGACCACCACACCGGCCGGGTTCGGGTGTTTGtct                                                                                                                                                  >  2:114053/1‑251 (MQ=255)                                                                                                    gCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAgcgc                                                                                                                                         >  1:179826/1‑251 (MQ=255)                                                                                                               ggcgcCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaag                                                                                                                              <  1:36564/250‑1 (MQ=255)                                                                                                                   ccGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcg                                                                                                                           >  1:27572/1‑250 (MQ=255)                                                                                                                    cGGGCGCGATTCGGCCAGTGCCTGCGTGAGCAGGACGGCATAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACATCGACGGTCGGCTTCCCGACGGTCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCAGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcgat                                                                                                                         <  1:11442/251‑1 (MQ=255)                                                                                                                       gggcgcTTCCGCCGGTGCCTGCGCGGGCAGGACGGCGTAGAGCCGGAGGACGTGGTCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCGCGGAAGCGCAggcgatcaaggcgatcaa                                                                                                                      <  2:96881/249‑1 (MQ=255)                                                                                                                               ccGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGaa                                                                                                               <  1:105964/250‑1 (MQ=255)                                                                                                                                           gcgcGAGCAGGGCGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACAGCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCTAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAATGCGATCAAGCGGGAAGAGATTGTGCAg                                                                                                   <  2:16328/250‑1 (MQ=255)                                                                                                                                             gcgAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCGGCCCTCGCCGGGCTGCTTCCCGACGGGCAACAGCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGc                                                                                                <  2:175709/251‑1 (MQ=255)                                                                                                                                                  cAGGACGGCGTAGAGCAGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTACCGACGGGCACCATCGCGCCGGAAGGATCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGGGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGCTGGAATCGGAAGCGCAGGCGATCAAGGAGCACAAGCGGGAAGAGATTGAGCAGGGAGatac                                                                                           >  1:262583/1‑250 (MQ=255)                                                                                                                                                   aGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATc                                                                                          <  1:45240/251‑1 (MQ=255)                                                                                                                                                        ggcgtgcagCCGGTTGACGTGCTGCTGCCGCCGGAGAGGGCAAAAGCGAACGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAAAATCGAGCCGGACGGTTCGGTGATCAAGGCCACGGCGATCGACAAGTCGGTGGTGGGAGAAGATGGCGTATACCACCACACCGGCCGGGGGCGGGCGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCggg                                                                                                                 >  2:233864/8‑223 (MQ=255)                                                                                                                                                        ggCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGTGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGGTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCggg                                                                                                                 <  1:233864/223‑1 (MQ=255)                                                                                                                                                           ggagagCCGGAGGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCGCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGAGCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCGCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATggtggt                                                                                  <  2:148229/249‑1 (MQ=255)                                                                                                                                                                  cGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATcggc                                                                           >  2:178864/1‑251 (MQ=255)                                                                                                                                                                       gACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATcggcggcgg                                                                      <  1:7808/251‑1 (MQ=255)                                                                                                                                                                          gTGAGCCTGCCGCCGGAGAACGCAAAAGCGGAAGGGCTGCCCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGCCCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACCCCGGCCGGGTGCGGGTGTTTGTCGCGGAAGCGCATGCGATCTAGGCGATCAAGGGTGAAGAGATTGTGCAGGGCGATATCATGGTGGTGACCGTCGGCGGGcc                                                                   <  2:58107/251‑1 (MQ=255)                                                                                                                                                                           tGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGAGATCGGCGGCGGGCCg                                                                  >  2:77420/1‑251 (MQ=255)                                                                                                                                                                               ccTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccg                                                              <  2:123285/251‑1 (MQ=255)                                                                                                                                                                               ccTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGGTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGGCGTcc                                                               <  2:244503/250‑1 (MQ=255)                                                                                                                                                                                 ggccgccGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccggc                                                            <  2:21031/250‑1 (MQ=255)                                                                                                                                                                                      ccgGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACAGCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGGGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCAGGTGTTTGTCTCGGAAGCGCAGGCGTGCAAGTCGATCAAGCAGGAAGAGATTGAGCAGGTCGATATCATGGGGGGGATCGGCGGCGGGGCGTcccgcgccgg                                                       >  2:52259/1‑251 (MQ=255)                                                                                                                                                                                         gagaAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccggcaccggcct                                                    >  1:131139/1‑249 (MQ=255)                                                                                                                                                                                         gagaAAGCAAACGCGAAAGGGCTGACCTGGCCGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCAt                                                    <  2:45069/251‑1 (MQ=255)                                                                                                                                                                                                  aaaGCGAAAGGGCTGACGTGGACGGGCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGGGATCAAGGCCACGGCGATCGACGCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACCCCGGCCGGGTGCGGGGGTTTGTCTCGGAAGCGCAGGCGAGCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGGACCGGCATGGAAGAGAc                                           <  2:237913/251‑1 (MQ=255)                                                                                                                                                                                                      cGAAAGGGCTGACCTCGTAGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCTGTCCGGCACCGGCATGGAAGAGACCTAc                                       <  1:114961/251‑1 (MQ=255)                                                                                                                                                                                                         aaGGGCCGACCTCGACGGTCTGCTTCCCGACTGGCAACATCGCGCCGGAAGGTGCGGTGAGCAAGGCCACGGCGATCGACCCTTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTGTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCTGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCTCCTGCATGGAAGATACCTACCAg                                    <  2:253376/251‑1 (MQ=255)                                                                                                                                                                                                             gCTGGCCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATGGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCa                                <  1:236768/251‑1 (MQ=255)                                                                                                                                                                                                                     cGACGGTCTGTTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCcgcg                        >  1:145171/1‑251 (MQ=255)                                                                                                                                                                                                                         ggTCTACTTCCCGCAGGGCAACATCGAGCAGGCAGGCTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGAGGGCGACGATGGCGTATACAACCACACCGGCCGGGAGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGCCGATCAAGCGGGAAGAGATTGTGAAGGGCGATATCCTGGAGGTGAACGGCGGCGGGACGGCCGGCACCGGCCTGGAAGAGAACTACCACCACACCTACGCGCTa                     >  2:253215/1‑250 (MQ=255)                                                                                                                                                                                                                              tcTTCCCGACGGGCAACATCGCGCCGGAAGGTCCGGTGATCAAGGCCACGGCGCTCGCCTCGTCGGCGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGCTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGTGCGATATCATGGTGGTGATCGGCGGCCGGCCGTCCGGCACCGGCATGGAAGAGACCTATCAGCTCACCTCCTCGCAAAAGCa                <  2:113029/249‑1 (MQ=255)                                                                                                                                                                                                                                ttCCCGACGGGCAACAACGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGAGGGCCGTCCGGCACCGGCATGGAAAAGACCTACCCGCTGAACCCCGCGCTAAAACatat             >  2:100391/1‑251 (MQ=255)                                                                                                                                                                                                                                 tCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGGCCTGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCTCGCTAAAGCata              <  2:164864/249‑1 (MQ=255)                                                                                                                                                                                                                                       cGGGCAACATCGCGCCGGAAGGATCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGAGGTGATCGGCGGCGGGCCGTCCGGCACCGGCGTGGCAGAGGCCTACCAGCTCACCTCCGCCCTAAAGCCTATCTCgggg      >  2:100538/1‑248 (MQ=255)                                                                                                                                                                                                                                         ggCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTgggg    <  2:192456/251‑1 (MQ=255)                                                                                                                                                                                                                                          gCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGc   <  1:111260/251‑1 (MQ=255)                                                                                                                                                                                                                                           cAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCa  <  1:71514/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  CGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCA  >  NC_000913/284292‑284776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑