Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,634,6610CG60.6% ‑4.6 / 39.2 33T75T (ACG→ACC) tfaQputative tail fiber assembly protein TfaQ
Reads supporting (aligned to +/- strand):  ref base C (9/4);  new base G (7/13);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 7.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.43e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATTTCCAGATCTGCAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACA‑GTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGCCATTATG  >  NC_000913/1634422‑1634889
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                    
aTTTCCAGATCTGCAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACA‑GTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCg                                                                                                                                                                                                                           <  1:258884/251‑1 (MQ=11)
  ttCCAGATCTGCAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACA‑GTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGCAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCgat                                                                                                                                                                                                                         >  2:157286/1‑251 (MQ=14)
          cTGCAGCATCCTGAAGCTGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACA‑GTTTTTCTGCTTCCGTATCCTTCACCCATGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTc                                                                                                                                                                                                                  >  1:102672/1‑250 (MQ=14)
          cTGCAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACA‑GTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACAGAGTTCAGAATTAAATAACGCGTCGACGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTc                                                                                                                                                                                                                  >  2:197705/1‑250 (MQ=14)
               gcatccgtaaGCGGCGCAATATGCTCACTGGCTACCTGCATCATGCTTTTTTTTGTTTCGTCCGCCTCCCGGATCCGGAACA‑GTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCGTATATTCCCCTCCCGGCGATAACCATGTAAAATGTTCCGGTAACGGACCGAGTGCATAAATAAATAACGCGTCGCCGGAATCCACGTCATAGTCGGTTTTACCCCaatggtcggcaacgtg                                                                                                                                                                                                            <  2:151168/243‑13 (MQ=11)
                   ccTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACA‑GTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATg                                                                                                                                                                                                         <  1:224576/250‑1 (MQ=11)
                              gcAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACA‑GTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtc                                                                                                                                                                                             <  2:3987/251‑1 (MQ=31)
                                         aCTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGCATC‑AGAACAAGTTTTTCTGATTCCGTCTCATTCACCCAGGATGTGACGTTACACTTCAGAAACTCCACTTACGGCGACAACCAGGCAACATTTTACGGTAACGAACCGAGTTCAGAACTAAACAACGCGTCAAAGGAAGCAACGGCCTAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTg                                                                                                                                                                                   >  1:17951/1‑250 (MQ=2)
                                            ggCTAGCTGCATCCGGCTTTTTTATGTTTATTACGCCTCCCGGATCCGGCACA‑GTTTTTCTGCTATCGTATCCTTAACCCAGGCAGTGCCGTTCCCATTCTGATATTCCCCTCCCGGCGATAACCCGGTAAAATTTTCCGGTAACGGACAGGGATCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTATTCAACGAGATGCCACGATGCCTCATAACTGTTGaaa                                                                                                                                                                                >  2:150297/1‑250 (MQ=255)
                                                      aTCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACA‑GTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCAcaa                                                                                                                                                                      >  1:216693/1‑248 (MQ=11)
                                                          ggattttttttGTTTCTTCCTCCTACCGGATCCGGTACA‑GTTTTGCTGCTCCCGTATCCTTCCCCCAGGCTGGGCCGTTCCACTTCTGAATCTCCCATTCCGACGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTTCCCCGACGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagcca                                                                                                                                                                 <  2:714/246‑1 (MQ=11)
                                                              tttttttGTTTCTTCCGCCTCCCGGATCCGGAACA‑GTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAagccagc                                                                                                                                                               <  2:192312/248‑1 (MQ=11)
                                                                   ttGTTTCTTCCGCCTCCCGGATCCGGAACA‑GTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAat                                                                                                                                                         <  1:70168/250‑1 (MQ=11)
                                                                           tcCGCCTCCCGGATCCGGAACA‑GTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCg                                                                                                                                                 <  1:217080/250‑1 (MQ=11)
                                                                                               cA‑GTTTATCTGCTTCCGCATCCTTCACGCAGGCTGAGCCGTTCCACTTCTGTTATTCCCCTCCCGGCGATACCCCGGTAACATTTTCCGGTAACGGACCGACTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGCTGCCACGATGCCTCATCACTGTTGAAAACAACCCCAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTg                                                                                                                            >  2:104920/1‑251 (MQ=11)
                                                                                                          gCTTCCGTATCCTTCACCCAGGCTGGTCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAAAAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGAGGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATGTCGGTACTGTTTgcaggcag                                                                                                                     <  2:255492/248‑1 (MQ=11)
                                                                                                               cGTATCCTTCACCCAGGCTGTGCCGTTCCCCTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCCCGTCATAGACGGTTTTACCCCGATGGTCTTCACCGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACCAAGCCCGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTAt                                                                                                             >  1:74626/1‑251 (MQ=11)
                                                                                                                    ccTTCCCCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCGGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAgg                                                                                                         <  2:20559/250‑1 (MQ=11)
                                                                                                                               gCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGc                                                                                             >  1:10989/1‑251 (MQ=31)
                                                                                                                                                             cccGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATAGCGGTACTGTTTGCAGGCAGACCGGTATGATGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTcc                                                               <  1:58149/250‑1 (MQ=11)
                                                                                                                                                              ccGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTcc                                                               <  2:28465/250‑1 (MQ=31)
                                                                                                                                                                ggCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCCACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGc                                                            >  1:135197/1‑251 (MQ=14)
                                                                                                                                                                     tAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCcagc                                                        >  2:189541/1‑250 (MQ=31)
                                                                                                                                                                      aaCCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCcagcag                                                      <  1:138615/251‑1 (MQ=11)
                                                                                                                                                                         cAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGCAATAAATAACGCGACGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGAtt                                                   >  2:45272/1‑251 (MQ=11)
                                                                                                                                                                                        ggTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGt                                    >  2:74371/1‑251 (MQ=31)
                                                                                                                                                                                                  cGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGgttgt                          <  1:157286/251‑1 (MQ=31)
                                                                                                                                                                                                            aataaataACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTAATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTc                >  1:262102/1‑251 (MQ=11)
                                                                                                                                                                                                                   aaCGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGAGGGTCGTCAACGAGATGCCACTATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAAGAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGc         <  1:230922/251‑1 (MQ=11)
                                                                                                                                                                                                                        gTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGCGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGCCAttt    >  1:218132/1‑250 (MQ=11)
                                                                                                                                                                                                                          cGCCGGAAGCCACGTCATAGACGGTTTTTCCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGCCATTATg  <  1:184081/251‑1 (MQ=11)
                                                                                                                                                                                                                          cGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGCCATTATg  <  2:45491/251‑1 (MQ=11)
                                                                                                                                                                                                                          cGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGCCATTATg  >  1:8486/1‑251 (MQ=11)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                    
ATTTCCAGATCTGCAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACA‑GTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGCCATTATG  >  NC_000913/1634422‑1634889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑