Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,883	+C	coding (667/687 nt)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,883	1	.	C	97.7%	129.8 / ‑3.5	43	coding (667/687 nt)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (24/18);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

GCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCC  >  NC_000913/3815639‑3816115                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         gcCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCggg                                                                                                                                                                                                                                     <  1:33482/251‑1 (MQ=255)              cAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCACCACCCCCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCcaaca                                                                                                                                                                                                                         >  1:8643/1‑250 (MQ=255)                gCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTGGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGa                                                                                                                                                                                                                      <  1:1059/251‑1 (MQ=255)                     gCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTAGGGCCAGAGCCAACAAGATGAGt                                                                                                                                                                                                                 >  1:24839/1‑251 (MQ=255)                      cGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTa                                                                                                                                                                                                                <  2:14272/251‑1 (MQ=255)                       gTTGAAGAAATCGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGt                                                                                                                                                                                                                 >  2:218860/1‑249 (MQ=255)                         tGAAGAAATTGGGGCTTTTGCGCCCGGATTTCAGCGTAAAGTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCTCTCCTCATCTTACTTTTCTACAGCCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGGTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATGCGA‑TTCCCCCTCTGGCCATAGCCAACAAGATGAGTAgg                                                                                                                                                                                                              <  2:1859/250‑2 (MQ=255)                          gaagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCGACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGctc                                                                                                                                                                                                            <  1:251344/251‑1 (MQ=255)                                         tttGCGCACGGATGTAAGCGGAACCACGACAAACTTTAACACTTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCAAGCATTCTCTCATCATATTACTTTTCTACACACAAACAAAAGGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGAGCCGACTTCTGAGTCGCTACAATGGATTAGA‑TTCCCCCTCGCGCCAGAGCCACCAAGATGAGAAACTCTTAATgggagtccgg                                                                                                                                                                                             >  2:154188/1‑244 (MQ=255)                                          ttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc                                                                                                                                                                                            >  2:201832/1‑251 (MQ=255)                                            gcgcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                                                                                                                                                                           <  1:99310/250‑1 (MQ=255)                                                ccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCt                                                                                                                                                                                      >  1:181155/1‑251 (MQ=255)                                                       tCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCTCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCGAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                                                                                                                                                                                <  1:157237/250‑1 (MQ=255)                                                             tAAACTCGCCAAAATTTAACACCTGCTTGCTCAGCGCAAATTCAATACACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGCCAAACACAAGGCGACTCATCAGTCGCCTTAAAACTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCTTGGGTGAACGGCTCGCCTTCTGCCGTCCCCt                                                                                                                                                                         >  2:155967/1‑251 (MQ=255)                                                                              aaCACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc                                                                                                                                                        >  1:26715/1‑251 (MQ=255)                                                                                 acCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCg                                                                                                                                                     >  2:179443/1‑251 (MQ=255)                                                                                 acCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCg                                                                                                                                                     <  1:318/251‑1 (MQ=255)                                                                                  ccTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGt                                                                                                                                                    >  1:190910/1‑251 (MQ=255)                                                                                                                ggccatggtatGGTTTCATGCCTTCGCTCCCCATCTTACTGTTCGACAGACAAAAAAAAGGCGACTCAGCAGTCGCCTTAAAAATCAATTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATGCGA‑TTCCCCCTCGGCCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCGCTGCGCTTCAATGCTTCGCTCGTCTTCGGTCATCACTACGTTCATGTCGGtct                                                                                                                      <  2:40659/243‑1 (MQ=255)                                                                                                                  cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTctct                                                                                                                    <  1:131935/251‑1 (MQ=255)                                                                                                                       atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGc                                                                                                               >  1:155661/1‑251 (MQ=255)                                                                                                                                cATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTc                                                                                                      >  1:71754/1‑251 (MQ=255)                                                                                                                                       tcactcctcATCTTACTTTTCTACAGACAACAACAAGGCGACTCATCAGTCGCCTTAAAATTCAGTTTGCAAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCCTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTCCACTTCAATGATGCGCCCGTCTTCGTTCATCACTACCCTCTTGTCGGTCTCTGCGGCAAAGTCTTCAACGTAt                                                                                               >  2:145435/4‑251 (MQ=255)                                                                                                                                               cATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCATAGTCTTCAACGAATTCCAGa                                                                                         <  2:96628/249‑1 (MQ=255)                                                                                                                                                aTCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTcc                                                                                            >  2:73181/1‑245 (MQ=255)                                                                                                                                                  cTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGc                                                                                     >  1:224507/1‑250 (MQ=255)                                                                                                                                                        tttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGAGTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTGCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCg                                                                               <  2:31246/250‑1 (MQ=255)                                                                                                                                                         ttCTACAGACAAAAAACAGACGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGAGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCAAGATCGCaacacgct                                                                             >  2:151359/1‑245 (MQ=255)                                                                                                                                                           cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTc                                                                           >  2:227847/1‑251 (MQ=255)                                                                                                                                                                  cAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGGTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTc                                                                    <  2:6744/251‑1 (MQ=255)                                                                                                                                                                      aaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTcaca                                                                 >  2:145103/1‑250 (MQ=255)                                                                                                                                                                                  catcatTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCGACAATGGATTCGA‑TTCCCCCGCGTGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACTGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCTGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGa                                                      <  1:32630/249‑1 (MQ=255)                                                                                                                                                                                  catcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGaa                                                     >  1:189138/1‑250 (MQ=255)                                                                                                                                                                                  catcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGaa                                                     >  1:184383/1‑250 (MQ=255)                                                                                                                                                                                      attCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTGCTGCGTCGCGACAATGGATTCGAGTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGGCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCTCTTCGCCGTTCACAATTCCGACATAAACTga                                                <  2:21384/249‑2 (MQ=255)                                                                                                                                                                                           ccTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTa                                           <  1:108593/251‑1 (MQ=255)                                                                                                                                                                                               aaaaaTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGTATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTCGCTCTTCATGGGTGAACGGCTCGCCGTCTGCCGTCCCCTGCACTGCAATTATGCGCCCGTCTTCGGTCATCGCGACGTTCATGTCGGTCGCTGCGGCATAGTCTTCAACGTATTCCATATCGCAAACCGCTTCGCCGTACACAATTCCGTCAGAAACTGCGGCTACCa                                        <  1:234108/250‑1 (MQ=255)                                                                                                                                                                                                          tGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATc                             <  1:227847/250‑1 (MQ=255)                                                                                                                                                                                                           gCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCgg                           >  1:183616/1‑251 (MQ=255)                                                                                                                                                                                                           gCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCAGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGGCTCTGCGGCAGAGTCTTCAACGTATTCCAAATCGCAAACCGCTTCGCCGTTCACAATTCCGCCAGAAACTGCGGCTACCATCCCTTTCATCgg                           >  2:200171/1‑251 (MQ=255)                                                                                                                                                                                                                   cgccTTCTGCGTCGCTACAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGttt                   >  1:110817/1‑251 (MQ=255)                                                                                                                                                                                                                        tCTGCGTCGCTACAACGGGTTCGA‑TTCCCCCTCGGGCCAGAGCCAACCAGATGAGGAGCTCTGCATGGGTAGACGGCTCGCCGTCTGCCGTCCCCTGCACTTCAAGGATGCGCCCGTCTTCGCCCATCACTACGTTCATGTCGGGCTCTGCAGCAGAGTGTTCAACTTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACGGCGGCTCCCATCCCGTTCATCGGATTGTTTTTCAg               <  2:99197/250‑1 (MQ=255)                                                                                                                                                                                                                                    cAATGGATTCGA‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTcc  >  1:35142/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         GCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCC  >  NC_000913/3815639‑3816115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑