Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,207,5490G.97.4% 161.7 / ‑4.2 39coding (179/606 nt)ttdBL(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (20/18);  minor base C (1/0);  total (21/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTG‑GTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGC  >  NC_000913/3207304‑3207794
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     
gtcgCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACCACCCCGATCAAAGCTGAAGATCTGCAAGATATTGGCGTCGGCGATGCGATCTCCCTGACCGGTACGCGG‑GTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGACCTGACACGTCCGATCCTTTACGATCTCAACGGCAAAGCGATTTGCCACGCTGCcccct                                                                                                                                                                                                                                                  >  2:218061/1‑250 (MQ=255)
              ctgGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCGGAAGATCTGCATGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTG‑GTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGCTTTTCCACGCTG‑CACCATCTTGCGCAAAAACg                                                                                                                                                                                                                                   >  2:229556/1‑251 (MQ=255)
                      tGCCGATCTCACCTTTGAACATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTG‑GTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACaa                                                                                                                                                                                                                            >  2:22003/1‑250 (MQ=255)
                           accccACCTTGGAAACTCTGCCACCCACCCGGAGCGCTTTATGAAAAAGATCCGGCCACCCCCGATCAAAGCGTAAGATCTGCAAGATATGCCCGCCGGCGTTGTGATCGCCCGGACCGGTACGCTGTGTG‑CC‑TGCCGCGACGTTTGTCCCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATGTTCCACGCTG‑CCCCATCGTGCGCGAAAACGGCGACAAATGGga                                                                                                                                                                                                                      <  2:189779/247‑1 (MQ=255)
                                              gTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCGGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTG‑GTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCgg                                                                                                                                                                                                       <  2:65682/247‑1 (MQ=255)
                                                   acacCCGGATCGGGTTATGAAAAAACTACCGACAACCCCGATCAAGGCTGAATATCGGCAAGATATTCGCGTCGGCGATGTGATCTACCTGCCCGGTACGATG‑TTGCCC‑TGCCGCGACGTTTGTCTCCGCCGGTTGATCGAACGGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CACCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTATCCGTCGGCCCTACAAc                                                                                                                                                                                              <  2:43150/251‑1 (MQ=255)
                                                                gtgtggAAAAAGCTCCGGACACCCCCGATCAAAGCTGAAGATCTGAAAGATTTTCGCGTCGGCGAGGTGATCAACCGGACCGGTACGCTG‑GTGACC‑GGCCGCGCCGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATg                                                                                                                                                                                 <  2:52114/246‑1 (MQ=255)
                                                                gTTATGAAAAAGATCCTGCCAACCCCGATCAAAGCTGAAGATCTGCAAGATATGCGCGTCGGCGATGTGATCTCCCTGACCGGTACGCTG‑GTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGTTCTCCGTCGGCCCGACAACCAGTATGCGTATg                                                                                                                                                                                 <  1:215746/251‑1 (MQ=255)
                                                                                  aCAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTG‑GTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGaa                                                                                                                                                               <  1:22003/251‑1 (MQ=255)
                                                                                               aaGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGCTCTACCTGAACGGTACGCTG‑GTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTATCCGTCGGCCCGACAACCAGTATGCGAATGGAAGGTTTTGAACGGGAGTTTATTGAGc                                                                                                                                                     >  2:5996/1‑248 (MQ=255)
                                                                                                     aaGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTG‑GGGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGc                                                                                                                                             <  1:199639/250‑1 (MQ=255)
                                                                                                      aGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTG‑GTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGt                                                                                                                                           >  1:17096/1‑251 (MQ=255)
                                                                                                       gATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTG‑GTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTg                                                                                                                                          >  1:112379/1‑251 (MQ=255)
                                                                                                             cAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTG‑GTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGCTTTGAACGTGAATTTATTGAGCAGACCGGCGTGaaaatg                                                                                                                                    >  1:129803/1‑248 (MQ=255)
                                                                                                                gATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTG‑GTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACtggtg                                                                                                                                 >  1:75952/1‑251 (MQ=255)
                                                                                                                 atatTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTG‑GTGCCC‑TGCCGCGACGTTTGTCACCGCCGTTTGTTCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGTTTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGCCTCCGTCGCCCCGACAACCAGAATGCGTATGGAAAGTTTTGAACGGGAATTTATTGAGCAGACCGGCGTGAAACtggggg                                                                                                                                >  1:197914/1‑248 (MQ=255)
                                                                                                                    tccgcgTCGGCGATGTGATCTACCTGACCGTTCCGCTG‑GGGCCC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACGGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCATACCGGCGTGAAACTGGTGGtt                                                                                                                              <  2:113182/248‑1 (MQ=255)
                                                                                                                     tCGCGGCGGCGAGGTGATCTCCCCAACCGGTACGCTG‑GTGCCC‑TGCCGCGTCGTTGGTCCCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTg                                                                                                                             <  2:61565/250‑1 (MQ=255)
                                                                                                                      cgcgTCGGCGATGTGATCTACCTGACCGGTACGCTG‑GTGACC‑TGCAGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGAATGCGTAAGGAAAGTTTTGAACGTGAATTTATTGAGCAGACAGGCGTGAAACTGGTGGTTGGc                                                                                                                           >  2:127474/1‑251 (MQ=255)
                                                                                                                        cgTCGGCGATGTGATCTACCTGACCGGTACGCTG‑GTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCa                                                                                                                          >  2:113628/1‑250 (MQ=255)
                                                                                                                               gATGTGATCTACCTGACCGGTACGCTG‑GAGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGAACGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGAGATTATCCACGCTG‑ACCCATCGTGCGTAAAAACGGCGACAAATGAGAGATGGTCTCCGACGGCCCGACAACCAGTCTCCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCTGTCCGGCGTGAAACTGGAGGTTggataagttgat                                                                                                                  >  1:143680/1‑241 (MQ=255)
                                                                                                                                    gATCTACCTGACCGGTACGTTG‑GTGACC‑TGCCCAGAGGTTTGTCAGCGCCGTTTGATCGCCATGACACGTCCGATCCCTTACGCTCTCAACGGCAAAGCGCTTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACAGGCGTGAAACTGGTGGTTGGCAAAGGGGGTATggg                                                                                                             >  2:254723/1‑251 (MQ=255)
                                                                                                                                         cccggcccGGTACCCTG‑GTTCCA‑TGCCGCGACGTTTGTCCCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATGTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATtgggacg                                                                                                         <  2:102821/244‑4 (MQ=255)
                                                                                                                                         aCCTGACCGGTACGCTG‑GTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGGATGGGGCCGc                                                                                                        >  1:125286/1‑251 (MQ=255)
                                                                                                                                          ccTGACCGGTACGCTG‑GTGACC‑TGCCGCGACGTTTGTCAACGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCt                                                                                                       >  1:138190/1‑251 (MQ=255)
                                                                                                                                               cccggtcggtG‑GTGACCCTGCCGCGCCTTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGGATGCGTATGGAAAGTTTTGAACGGGAAGTTATTTAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGGATGGGGCCGCTGAcc                                                                                                   <  2:129803/242‑1 (MQ=255)
                                                                                                                                                   tACGCTG‑GTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAACGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCga                                                                                                 >  1:33373/1‑248 (MQ=255)
                                                                                                                                                         g‑gTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTg                                                                                        <  1:84506/251‑1 (MQ=255)
                                                                                                                                                                  tGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGaa                                                                                  >  1:184423/1‑250 (MQ=255)
                                                                                                                                                                        gACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAg                                                                           <  1:113628/251‑1 (MQ=255)
                                                                                                                                                                                    cgccgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAAATGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAtgtg                                                               >  1:85658/1‑251 (MQ=255)
                                                                                                                                                                                     gccgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGCATGCGTATGGAAAGTTTTGATCGTGACTTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAtgtgt                                                              >  1:105428/1‑250 (MQ=255)
                                                                                                                                                                                           tgagtgAACTGAAACGTCCTATCCCTTACGATCTCAACGGCAAAGCGATTTTCCTCGCTG‑CCCTATCGTGCGCAAAAACGGCGACAAAAGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTTAGCAGACCGGCGTGAAACTGGTGGTTGGAAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACTTGTGATTTTc                                                         <  2:143680/245‑1 (MQ=255)
                                                                                                                                                                                                     gAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCt                                               <  2:255033/250‑1 (MQ=255)
                                                                                                                                                                                                                 cccTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCACAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGa                                                                                                                                                                >  2:44603/1‑125 (MQ=255)
                                                                                                                                                                                                                 cccTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGa                                                                                                                                                                <  1:44603/125‑1 (MQ=255)
                                                                                                                                                                                                                              aaCGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTg                     <  2:75952/251‑1 (MQ=255)
                                                                                                                                                                                                                               aCGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTgg                    <  2:33373/251‑1 (MQ=255)
                                                                                                                                                                                                                                    aaaGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAag                 >  2:50168/1‑249 (MQ=255)
                                                                                                                                                                                                                                                  ccgcggCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAAGTCAAGGCGCTACATGTGATGTTCCCGGCAGGCTGCGCGGTGCTGGCGGCGACCCAGGTGGAAGAGATTTAAGAAGTGc  <  2:159587/247‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     
GTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTG‑GTGACC‑TGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGC  >  NC_000913/3207304‑3207794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑