Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,111,2590G.93.8% 64.0 / ‑2.0 16coding (718/900 nt)rfbDdTDP‑4‑dehydrorhamnose reductase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (7/8);  total (8/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGT  >  NC_000913/2111037‑2111484
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                 
ttCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGAGTTAGTTTAACGCCAACCTGCAAGTCAGGCAAGACAAGCGCAAAGCTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGGTATGCGGACGACGAGCTGGTGTCGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCacaa                                                                                                                                                                                                       >  1:174730/1‑247 (MQ=255)
           ccaccaTCACGAACAAGATGCAAAAACTCTTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCATTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGCGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGGGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCgcagc                                                                                                                                                                                            >  2:52399/1‑250 (MQ=255)
                aTCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTTCGCGCCTCTTCAAAAACCAGCGCAGCATAATc                                                                                                                                                                                      <  2:158736/251‑1 (MQ=255)
                  cACGAACACGATGCAAAACCTATTCAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTAAAGACAACCTGCCAGTCAGGCAAGACAAGCGCCAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGAGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCA‑CGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACAAGCGCAGCATAATCGt                                                                                                                                                                                    >  2:245530/1‑251 (MQ=255)
                         aaGATGCAAAAACTATTAAATGGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCGTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGt                                                                                                                                                                             <  1:255294/251‑1 (MQ=255)
                          aGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCAGTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTGTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCTTTTAGCTTGTTGAGTGCAAGGG‑AATGCCTGCGTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGtt                                                                                                                                                                            <  2:247045/251‑1 (MQ=255)
                                             ttGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTAcc                                                                                                                                                          >  2:57057/1‑250 (MQ=255)
                                                  gTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATgg                                                                                                                                                    >  1:76032/1‑251 (MQ=255)
                                                                         cATTCGTTTCACGCAAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCTTTGAGCGTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGttt                                                                                                                             <  2:90876/251‑1 (MQ=255)
                                                                           ttCGTTTCACGCCAAACTGCCAGTCAGGCAAGCCAAGCGAAAAGTTCTGCTGAAATTTTTCTGCATTACGGCGCGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTAt                                                                                                                           >  2:133543/1‑251 (MQ=255)
                                                                                 tCACTCCAACCTGCCAGTCAGGCAAGACAAGCGAAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTGATGTGGACGAGGAGCTGGTGTAGGATAGGCTGTTGGTGGTACTGCGTTGAGCTTGGTGAGTGCAAGGG‑AATGCCTGCTTGGCGCGCCTCTACAAAAACCAGCGCGGCATAATCGTCCCAGGTTGTGGTACCACTGGTTACCAAATGGTACAAGCCTGCGACATCCGGGTTATTCAGt                                                                                                                      <  2:125759/250‑1 (MQ=255)
                                                                                                 gTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCAtg                                                                                                     <  1:116994/251‑1 (MQ=255)
                                                                                                                                                    agagTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGGTGAGTGCAAGGG‑AATGCCTGCTTGGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCAGGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATc                                                  <  1:133543/251‑1 (MQ=255)
                                                                                                                                                              ggACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTTCGTTGAGCTTGTTGAGTGCAAGGG‑AAAGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTTCAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCATCCAGCAGTTCAGCACCTGTTGGCGCACCAATCTTATCGTTAATAAc                                         <  2:208395/250‑1 (MQ=255)
                                                                                                                                                                                           tgttgGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCACAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACg           >  2:93666/1‑251 (MQ=255)
                                                                                                                                                                                                    tGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGt  >  1:176333/1‑251 (MQ=255)
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                 
TTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGT  >  NC_000913/2111037‑2111484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑