Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,563,778	(G)6→5	coding (148/258 nt)	ytiA ←	uncharacterized protein YtiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,563,778	0	G	.	100.0%	84.3 / NA	19	coding (148/258 nt)	ytiA	uncharacterized protein YtiA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (14/5);  total (14/5)

ACTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTC  >  NC_000913/4563536‑4564023                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      acTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTc                                                                                                                                                                                                                                                >  1:142549/1‑249 (MQ=255)          aTGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATACGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCAAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGc                                                                                                                                                                                                                                      >  1:262704/1‑250 (MQ=255)                                         gTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACgggg                                                                                                                                                                                                       >  2:394/1‑250 (MQ=255)                                                      aaTACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGAACTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTgcagc                                                                                                                                                                                         >  1:244668/1‑247 (MQ=255)                                                        tACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGAcgcg                                                                                                                                                                                        >  1:108450/1‑250 (MQ=255)                                                         acgttttGTCCCGTTAAACCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAAGTGACGCAGTCAGCCCAGGGATCACCCATTGTATGTCCGCGCCTGGGG‑ACAGGTCAGCAAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAAGATCGCTGACGCGa                                                                                                                                                                                       <  2:153239/247‑1 (MQ=255)                                                              ttGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGGCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGt                                                                                                                                                                                  <  2:112173/250‑1 (MQ=255)                                                                 tCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATg                                                                                                                                                                              >  1:28420/1‑251 (MQ=255)                                                                                 aaGGCAAATCAGTAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACg                                                                                                                                                              >  1:33330/1‑251 (MQ=255)                                                                                  aggaaaaTCAATAAGATATCGACAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGCGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGc                                                                                                                                                             >  2:27276/5‑251 (MQ=255)                                                                                                                            aaTGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGaaataaaa                                                                                                                    >  1:169475/1‑250 (MQ=255)                                                                                                                               gAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGaaataaaaataa                                                                                                                <  1:253944/251‑1 (MQ=255)                                                                                                                                aaTCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGaaataaaaataa                                                                                                                >  1:196823/1‑250 (MQ=255)                                                                                                                                                    cccGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTc                                                                                           >  1:138862/1‑251 (MQ=255)                                                                                                                                                       gTAATGTGCACTGCCGCGCCAGACACCATCCTGCTGGCAATAAAGTGACTCAGTCAGCCCAGTGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTc                                                                                        <  1:138241/251‑1 (MQ=255)                                                                                                                                                                   gCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATCTCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAAATCTTTCATCGGCGTGGCCTCACTCGTTgtcc                                                                             >  1:22167/1‑247 (MQ=255)                                                                                                                                                                                                aaaTGGACCCAGTCAGCCCAGGGATCACCCATTGTATTCCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGGGGAGTGAAAGAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTGGACCAATTACCGCCGCGCCAATTGCGCCAGCaaa                                               <  2:108450/251‑1 (MQ=255)                                                                                                                                                                                                           gtcagCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATc                                      >  1:159673/1‑249 (MQ=255)                                                                                                                                                                                                                                  tATTTCCTCGCCGGGGG‑ACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGGTGGGTATTTACCGGCATTCGc             >  1:119583/1‑251 (MQ=255)                                                                                                                                                                                                                                              cgggggACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCCGGATGGGTATTTACCGGCATTCGCAGGTGAGATTc  >  1:229114/2‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      ACTGCCACAATGGCAAAAAGAATCACAATAATGGAGATGCGTTTACTTAACGAAATACTTTTTGTCACGGTAATCCCTTTAAGGCAAATCAATAAGATATCGAGAAGTATACGTAGCGACCTTAATGAATCCTTAACGCGGAGAAACCCCGTAATGTGCAATGCAGCGCCAGACACCATCCTGCTGGCAATAAATTGACTCAGTCAGCCCAGGGATCACCCATTGTATTTCCTCGCCGGGGGGACAGGTCAGCCAAATGCATAACCCGCAGCCGCCGCGTAAATCACGGGGAATATCGCTGACGCGAAAAGTCATGCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTC  >  NC_000913/4563536‑4564023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑