Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,429,766	C→G	intergenic (+211/‑98)	fklB → / → cycA	peptidyl‑prolyl cis‑trans isomerase FklB/D‑serine/alanine/glycine/:H(+)symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,429,766	0	C	G	100.0%	201.2 / NA	52	intergenic (+211/‑98)	fklB/cycA	peptidyl‑prolyl cis‑trans isomerase FklB/D‑serine/alanine/glycine/:H(+)symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (23/29);  total (23/29)

TTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGA  >  NC_000913/4429527‑4430008                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   ttGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGAt                                                                                                                                                                                                                                         >  1:216226/1‑251 (MQ=255)   gAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATg                                                                                                                                                                                                                                        <  1:487724/250‑1 (MQ=255)        cGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTcc                                                                                                                                                                                                                                   <  1:650830/250‑1 (MQ=255)                 ggAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATAc                                                                                                                                                                                                                             >  1:216504/1‑247 (MQ=255)                  gAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGAtt                                                                                                                                                                                                                        <  1:112258/251‑1 (MQ=255)                    aaTCCTCTAAGCAGCTCTTTCTGTTCCCCTCGAACGTGAGTGGAGCAGGCATTCAGCAATAACCCCTTCAGTTGGCCAAACGGCTCTATTTTGTTGTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGAGCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATTAGTCCCGATACAGATtcg                                                                                                                                                                                                                      <  1:434693/251‑1 (MQ=255)                            aaGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTAt                                                                                                                                                                                                                <  1:143983/249‑1 (MQ=255)                                  cgcATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCACTAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAgact                                                                                                                                                                                                        >  2:33300/1‑251 (MQ=255)                                          gTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAg                                                                                                                                                                                                <  1:461895/251‑1 (MQ=255)                                            tCCCCTCGCACGAGAGGGGAGCAGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGc                                                                                                                                                                                              >  2:266859/1‑251 (MQ=255)                                                cTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGt                                                                                                                                                                                           >  1:623681/1‑250 (MQ=255)                                                   gAACGAGAGGGGAGCAGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTGCGCGTTATCACCGTGATATGACACTCACCTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTagag                                                                                                                                                                                       <  2:263606/251‑1 (MQ=255)                                                    aaCGAGAGGGGAGCAGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGc                                                                                                                                                                                      >  2:205554/1‑251 (MQ=255)                                                     aCGAGAGGGGAGCAGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGCCCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGcc                                                                                                                                                                                     <  2:324877/251‑1 (MQ=255)                                                          agGGGAGCAGGCATTCAGCAATAAATCCTTCAGTTTGCCAAACGGAGCTATTTTGTGATGCAAAGCCCCCGTAAGCGTGTATTTTTGTGAGCTGTTTAGCGTTATCACCGTGATATGACAATCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAATATTCATCCCGAGAATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGACTGaac                                                                                                                                                                                >  1:121740/1‑251 (MQ=255)                                                            gggAGCAGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGaacaa                                                                                                                                                                              <  1:54441/251‑1 (MQ=255)                                                                  aGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACacaga                                                                                                                                                                        <  2:526067/251‑1 (MQ=255)                                                                             aaTAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAgg                                                                                                                                                              >  2:304725/1‑250 (MQ=255)                                                                             aaTAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGga                                                                                                                                                             <  1:304725/251‑1 (MQ=255)                                                                                   cccTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGaaa                                                                                                                                                        >  2:179786/1‑250 (MQ=255)                                                                                             tGCCAAACGGCACTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAAACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGt                                                                                                                                              >  2:56893/1‑250 (MQ=255)                                                                                                cAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATgga                                                                                                                                              >  1:113617/1‑246 (MQ=255)                                                                                                 aaaCGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGc                                                                                                                                                                                      <  2:94681/206‑1 (MQ=255)                                                                                                 aaaCGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGc                                                                                                                                                                                      >  1:94681/1‑206 (MQ=255)                                                                                                 aaaCGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGt                                                                                                                                              >  1:219484/1‑246 (MQ=255)                                                                                                    cGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAgg                                                                                                                                      <  1:14968/251‑1 (MQ=255)                                                                                                                gggtTGCAAAGACCCCGTAATCGTGTATTTTTGTGAGCGGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACAAAAAAGTAACATTAGATCTAAAGCTTAGTATTGATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGACTCGTCGTAGCATAGACTGACTAAAGGCCGTAGAGCCCGAACAACACAGACAGGTGCAGGAAGAAAAAACCATGGTAGATCAGGTAAAAGGCGtt                                                                                                                           <  2:219604/248‑1 (MQ=255)                                                                                                                  gtTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGcc                                                                                                                        <  1:266859/251‑1 (MQ=255)                                                                                                                     gCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATc                                                                                                                                         <  1:130141/231‑1 (MQ=255)                                                                                                                     gCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATc                                                                                                                                         >  2:130141/1‑231 (MQ=255)                                                                                                                                         tATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAg                                                                                                                                <  1:702267/220‑1 (MQ=255)                                                                                                                                         tATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGAAACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAg                                                                                                                                >  2:702267/1‑220 (MQ=255)                                                                                                                                                  gAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTAGTCATCCCGCGTATTGTTAGCTAATATCGATGATTCCCGATACAGATGCTTCGTATCATAGACTGACTAAAGGCCTTAGAGCCTGAACAACACAGACAGGTACAGGAATAAAAAAACATGGTAGAGCAGGTAAAAGGCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTAc                                                                                         <  2:299083/250‑1 (MQ=255)                                                                                                                                                                     ccGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTCTTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACacagaca                                                                                                                                                                      >  1:48116/1‑154 (MQ=255)                                                                                                                                                                     ccGTGATATGACACTCACTTTAAACATAAAATTAACATGAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTTAACAACacagaca                                                                                                                                                                      <  2:48116/154‑1 (MQ=255)                                                                                                                                                                                          aaaCATAAAATTAACATTAGATCTAAATCTTAGTATGCATCCCTCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTAACATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGGCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCAt                                                 <  1:264227/250‑1 (MQ=255)                                                                                                                                                                                          aaaCATAAAATTAACATATGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAAGAGCGATGAGTCCCGATACAAATTCTTCGTATCATAGACTGACTAAAGTCCGTAGAGCCTGAACAACACAGACAGGTACATGAAGTAAAATACAGTTTAGATCATGTTAAAGTCGTTGCCGCTGATCATGCTCCTGCTGAAAAGTTGCTACGGCTCAATCTCACATCCCGTCATTTTCAACTTATGGCCAt                                                 <  2:600515/250‑1 (MQ=255)                                                                                                                                                                                            aCATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATAGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGTCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTgg                                              <  2:141979/251‑1 (MQ=255)                                                                                                                                                                                              aTAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGCCATATTCAGCTTATTGCCATTGGCg                                            >  2:695431/1‑251 (MQ=255)                                                                                                                                                                                              aTAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCg                                            <  1:231456/251‑1 (MQ=255)                                                                                                                                                                                                        cATTAGATCTAAATCTTAGTATTCAGCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTgg                                  <  1:33300/251‑1 (MQ=255)                                                                                                                                                                                                         aTTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGt                                 <  1:637141/251‑1 (MQ=255)                                                                                                                                                                                                                cTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCAtt                                    >  1:208431/1‑241 (MQ=255)                                                                                                                                                                                                                cTAAATCTTAGTATTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCAtt                                    <  2:208431/241‑1 (MQ=255)                                                                                                                                                                                                                  aaaTCTTAGTATTCATCCCGCGTGTTGGTAGCTAATATCGATGCGTCCCGATACAGATTCGTCGCATCATAGACGGACTAAAGGCCGGAGAGCCTGAGCAACACAGACAGGCACAGGAAGAAAAAAAGATGGTAGATCAGGTAAAAGGCGTTGCCGATGGTCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGCCCAATTCAGCATATTGCCATTGGCGGTGccccttgtcagtgggtt                        >  2:3169/1‑236 (MQ=255)                                                                                                                                                                                                                   aaTCTTAGGAGTCATCCCGCGTAATGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCAGAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATAGTCAGCGTATTGCCATTGGCGGTGCCATTGGTACGGGgttgt                       <  2:216504/251‑1 (MQ=255)                                                                                                                                                                                                                        tAGTATTCAGCCCGCGTAGTGTTAGCTAATAGCGATGAGGCTCGATACAGATTCGTCGTCTCATAGACTGAATAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAGAAAAACATGGGAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTTCGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGCTGCCATTGGTACGGGGTTGttt                     <  1:488459/248‑1 (MQ=255)                                                                                                                                                                                                                            aTTCATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTAAGGGGGTGTTTTTGGGGt              >  2:53624/1‑251 (MQ=255)                                                                                                                                                                                                                               cATCCCGCGTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTCGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTc                                                                                                           >  2:98454/1‑155 (MQ=255)                                                                                                                                                                                                                               cATCCCGCGTATTGTGAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTc                                                                                                           <  1:98454/155‑1 (MQ=255)                                                                                                                                                                                                                                     gcgTATTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTTCCCTTTGTAAGGGGGTGTTTTTTGGGGCTGGcgaa      >  1:589588/1‑247 (MQ=255)                                                                                                                                                                                                                                         aTTGTTAGCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACAGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTAAGAGGGTGTTTTTTGGGGCTGGCAAAAcaa  >  1:414473/1‑248 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   TTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGA  >  NC_000913/4429527‑4430008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑