Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,347,395	A→T	intergenic (‑69/+9)	fumB ← / ← dcuB	fumarase B/anaerobic C4‑dicarboxylate transporter DcuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,347,395	0	A	T	100.0%	230.1 / NA	66	intergenic (‑69/+9)	fumB/dcuB	fumarase B/anaerobic C4‑dicarboxylate transporter DcuB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (34/32);  total (34/32)

AAGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAGGATGTAATAACCGTAGCAAGCCGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCG  >  NC_000913/4347149‑4347641                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       aagaagGGTCGTGAAAGGCTTGCTGCGCCGGCAGGTTCAGGGCTTCTGGTCCCACGTTCAGGATGGTTGCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGGGCCTGGTAGATAAAGGGTTAGTTTGACATAGCTTCCAGCCTGTAACTTTGTATTTGTTATACGAAAAGGGCGTGCGGCGTAGTCACGGGCGGCACGCTAAGt                                                                                                                                                                                                                                                    <  2:454218/251‑1 (MQ=255)    aagCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGATGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGACTGGAAGATAAAGGGGTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGc                                                                                                                                                                                                                                                  >  2:626274/1‑250 (MQ=255)      gCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCAtt                                                                                                                                                                                                                                               <  1:231555/251‑1 (MQ=255)       cgtcgtGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCCCGGGCGGCACGCTACGTGCAttt                                                                                                                                                                                                                                              >  2:375846/1‑251 (MQ=255)            tGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAACATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTtata                                                                                                                                                                                                                                           >  1:308081/1‑249 (MQ=255)             gAAAGGCTTGCTGCGCCAGCAGGGTCTGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGa                                                                                                                                                                                                                                        <  1:21960/251‑1 (MQ=255)              aaaGGATTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGCAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGCTAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCCCGCTAAGTGCATTTATAAGa                                                                                                                                                                                                                                        >  2:695437/1‑250 (MQ=255)               aaGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAAc                                                                                                                                                                                                                                      <  1:304192/251‑1 (MQ=255)                aGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGACAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAAcc                                                                                                                                                                                                                                     >  2:53090/1‑251 (MQ=255)                 ggCTTGCTGCGCCAGCAGGGTCAGGGCGTCTGGTTCCACTTTCAGGGTGGTTTCGCCGTCGAAGTCGGCAACGCTATCGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGGATATAAAGGGTTTTTGTTACATAGCTTCCAGCCTGTAACTCTGTATTTGTTAATCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAcccc                                                                                                                                                                                                                                    <  2:31227/251‑1 (MQ=255)                     tgctgaGCCAGCAGTGTCAGGGCTTCTGGTACCACTTTCAGGATGGTTTAGCTGTCTGAGTCGGCAATGCTAACGTAAACGGAAGTGAGTAGATAGAATTCGGTATTGTCTTTTCCCAACGTGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGAGTGCCGCGTAGTCACGGGTGGCACTCTAAGTGCATTTATAAGAAcccttc                                                                                                                                                                                                                                 <  2:606321/250‑4 (MQ=255)                                   ggTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCAGTTATAAGAACCCGTACATCGCGGCGAAGAt                                                                                                                                                                                                                  <  2:105843/251‑1 (MQ=255)                                              cTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGGGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGACGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACCTCGCGGCGAAGATCCAGCCGa                                                                                                                                                                                                          >  1:462341/1‑248 (MQ=255)                                                  ttCCAATTTAAGGGTGGTTTCGCCGTCGAAGACGGCAACGCTAACGTAATCGGCAGGGAGTAGATAGCATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCATGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCCTTTATAAGAACCCGTACATCCCAGCGACGATCCAGCCGAACACGcc                                                                                                                                                                                                   >  2:588273/1‑250 (MQ=255)                                                   tCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCa                                                                                                                                                                                                   <  1:420179/250‑1 (MQ=255)                                                    ccACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTAGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTATGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACg                                                                                                                                                                                                 >  2:193844/1‑251 (MQ=255)                                                         tacagtaTGGTTTCGCCGTCGAAGTCGGCAACGCTCACGTAATCGGAAGTGAGTAGATTTTATTCGTTATTGTCTTTCCCCATTGCGAAAGGTGCCTGGTAGACAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGGGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACTCTTACATCGCGGAGAAGCTCCAGCCGAAGACGCACGATAc                                                                                                                                                                                             <  1:330879/244‑1 (MQ=255)                                                           cAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCt                                                                                                                                                                                          >  1:618849/1‑251 (MQ=255)                                                            aGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTc                                                                                                                                                                                         <  1:695437/251‑1 (MQ=255)                                                                                 tCGGCAACGCTCACGTAATCGGTAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGATGCCTGGTAGATAAAGGGTTTGTTTGACCTAGCTTACAGACTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTAACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGc                                                                                                                                                                       >  2:610058/1‑248 (MQ=255)                                                                                  cGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGa                                                                                                                                                                    >  1:707661/1‑250 (MQ=255)                                                                                                 aaTCGGAAGTGAGTAGATAGTATGCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGGTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATGTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtg                                                                                                                                                     <  2:618849/250‑1 (MQ=255)                                                                                                             gTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGAc                                                                                                                                        >  2:478429/1‑251 (MQ=255)                                                                                                              gaGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATGTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGAcc                                                                                                                                       <  2:147557/250‑1 (MQ=255)                                                                                                               aGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGAcc                                                                                                                                       <  1:610058/250‑1 (MQ=255)                                                                                                                    gTATTCGGTATTTTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTTTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCGCACACCAATCAACCCCGGCAGAATAAAGCTTTGGTTGATGACGAAGCGACCGAtgtg                                                                                                                                 <  2:649731/251‑1 (MQ=255)                                                                                                                    gTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGAtgtg                                                                                                                                 <  2:248153/251‑1 (MQ=255)                                                                                                                    gTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGAtgtg                                                                                                                                 <  2:51946/251‑1 (MQ=255)                                                                                                                           gTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTAGTTGTTAGTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGgtggtg                                                                                                                          <  1:357749/251‑1 (MQ=255)                                                                                                                             aTTGTCTTTCCCCATCGGGAAAGGTGCCTGGCAGATAAAGGGCTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCAAGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGAAACGCTCACACCAATCAACCCCGGCAGCATAAAGCTGTGGTTGATGACGCAGCGACCGATGTGGGTGGTGcc                                                                                                                        >  1:623219/1‑251 (MQ=255)                                                                                                                             aTTGTCTTTCCCAATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGCAGACGCACGATACGCTCCCACCAATCAACCCCGGCAGAATAAAGATGTGGTTGATGACGAAGCGACCGATGTGGGTGGAGcc                                                                                                                        >  2:341967/1‑251 (MQ=255)                                                                                                                                         cATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCaaa                                                                                                            <  1:394866/251‑1 (MQ=255)                                                                                                                                           tCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGCCCGATGTGGGTGGTGCCGGAACGGTCAAACt                                                                                                          >  2:693859/1‑251 (MQ=255)                                                                                                                                                 aaGGTGCCTGGTAGATAAAGGGTTTGTTTGACATATCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCg                                                                                                                                                                         <  1:343308/182‑1 (MQ=255)                                                                                                                                                 aaGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCg                                                                                                    >  1:315432/1‑251 (MQ=255)                                                                                                                                                 aaGGTGCCTGGTAGATAAAGGGATTGTTTGACATAGCTTCTAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATAGCGGCGAAGATCCAGCCGAAGACGCACGATACGATCACACCAATCAAcccag                                                                                                                                                                         >  2:343308/1‑180 (MQ=255)                                                                                                                                                    gTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGGGGGGGGTGCCGGAACGGTCAACCTGCATCGCTg                                                                                                 >  2:77019/1‑251 (MQ=255)                                                                                                                                                     tGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCCACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGc                                                                                                >  1:587601/1‑251 (MQ=255)                                                                                                                                                           gTAGATACAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGATATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATAAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGGGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGcc                                                                                               >  2:438320/1‑246 (MQ=255)                                                                                                                                                              gATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACGCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACGGATGTGGGTGGTGCCAGAACGGTCAAACTGAATCGCGGCCAGATCGCt                                                                                       <  1:590355/251‑1 (MQ=255)                                                                                                                                                               aTAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAATCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTc                                                                                      >  1:56947/1‑251 (MQ=255)                                                                                                                                                                             tgACATTGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGTTAAGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGCCCGATGTGGGTGGTGCCGGACCGGTCAAACTGAATCGATGCCAGATCGCTCGGATAAGTCgcc                                                                          >  2:438452/1‑247 (MQ=255)                                                                                                                                                                              gACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAg                                                                        <  1:478429/250‑1 (MQ=255)                                                                                                                                                                              gACATAGCTGCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAg                                                                        <  1:626274/250‑1 (MQ=255)                                                                                                                                                                                   aGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCTCGGCCAGATCGCTCGGATAAGTCGGCAGGATGta                                                                  <  2:604421/251‑1 (MQ=255)                                                                                                                                                                                         cAGCCTGTAACTCTGTATTTGTTATTCGAAAATAGCGTGCCGCGTAGTCAAGGGAGGCCCGCTCAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAGGATGTAATAAcc                                                            >  2:111907/1‑251 (MQ=255)                                                                                                                                                                                          aGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAGGATGtaataaacg                                                           >  2:393097/1‑248 (MQ=255)                                                                                                                                                                                             ctggaaCTCTGTATATGTTATTAGAACATGACGTGCCGAGTAGTCACGCGCGGCACGCTAAGTGCATTTATAAGCACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGAAAGAAACGCTCACACCAATCAACCCCGGCAGCATATCGCTGTGGTTGACGACGAAGCGACCGATGTGGGTGGTGACGGAACTGGCACACTGAATCGCTGCCAGATCGCTCGGATACGCCGGCAGAATGTAATTACCGTa                                                         >  2:458656/5‑250 (MQ=255)                                                                                                                                                                                             cTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGCTCAGTCGGCCGGATGTAATAACCGt                                                          >  1:431244/1‑249 (MQ=255)                                                                                                                                                                                                tAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAGGATGTAATAACCGTAGCa                                                      >  1:495935/1‑250 (MQ=255)                                                                                                                                                                                                  aCTCTTTATTTGTTATTCGAAACTGGCGGGCCGCGTAGTCGCGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCGCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGAGCGCTCGGATAATTCGGCAGTATGGAATAACCGTAGCAAGc                                                   <  2:410108/251‑1 (MQ=255)                                                                                                                                                                                                   ctctGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGACCGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAGGATGTAATAACCGTAGAAAGcc                                                  >  2:189704/1‑251 (MQ=255)                                                                                                                                                                                                     ctGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAGGATGTAATAACCGTAGCAAGcc                                                  <  1:375846/249‑1 (MQ=255)                                                                                                                                                                                                             gTTATTCGAAATTGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATAAGCTCACCCCAATAAACCCCGGCAGAATAAAGCTGTGGTTGCTGACGCAGCGACCGATGTGGGTGGTGCCGGACCGGCCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAGGATGTATTAACCGTAGCAAGCCggggcgtaat                                        >  1:433971/1‑243 (MQ=255)                                                                                                                                                                                                               tATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTGATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTTGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAGGATGTAATAACCGTAGCAAGCCGGTGcgtaatg                                       <  2:433971/250‑7 (MQ=255)                                                                                                                                                                                                                aTTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAGGATGTAATAACCGTAGCAAGCCGGTGCTGAAGCCa                                     <  1:193844/251‑1 (MQ=255)                                                                                                                                                                                                                aTTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGAACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTAGGCAGGATGTAATAACCGTAGCAAGCCGGTGCTGAAGcc                                      >  1:308234/1‑250 (MQ=255)                                                                                                                                                                                                                  tCGAAAATGGCGTGACGCGAAGCCACGGGCGGCACGCTAAGTGAATGTAGGAGAACCCGTACATAGCGGCGAAGATCCAGCCGACGACGCACGATACGCTAAAACCAATAAACCCAGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGCTGTGGGCGGTGCAGGAACGGTCAAACTGAATCGCTGCCAGCTCGCTCGGATAAGTGGGCAGGATGTAAAAACCGTAGCacgacgcggctgaagcac                                     >  2:558062/1‑232 (MQ=255)                                                                                                                                                                                                                   cGAAAATGGGGGGCCGGTTAGTCACGGGCGGCCCGCTAAGTGCATTTATAAGAACCCGTACAGCGCGGAGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGGGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAGGATGTAATACCCGTAGCAAGCCGGTGCTGAAGCCACGa                                  <  2:379984/251‑1 (MQ=255)                                                                                                                                                                                                                       aaTGGCGTGCCGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGCACCCGTACATCGCGGCGAAGATCCAGCCGCAGACGCACGATACGCTCACACCAATCAACCCCGGCCGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGCCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAGGATGTAATAACCGTAGCAAGCCGGTGCTGAAGCCACGCTGt                               >  1:70255/1‑250 (MQ=255)                                                                                                                                                                                                                            cGTGCCGCGTAGTCACGGGCGGCACGCTAAGAGCATTTTTAAGAACCCTTACATCTCGGCGAAGAGCCAGCCGAAGACGCACGATACGCTCACACTAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAATCGACCGAGGTGGGTGGTGCCGGTACGGTCAAACTTAATCGCTGCCAGATCGCTCGGATAAGTCGGCAGGATGTAATAACCGTAGCAAGCCGGTGCTGAAGCCACGATGTATGCCg                         <  1:697643/251‑1 (MQ=255)                                                                                                                                                                                                                                ccGCGTAGTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAGGATGTAATAACCGTAGCAAGCCGGTGCTGAAGCCACGATGTATGCCGGATc                     >  1:534561/1‑251 (MQ=255)                                                                                                                                                                                                                                       gTCACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAGGATGTAATAACCGTAGCAAACCGGTGCTGAAGACACGATGGATGCCGGATCAACGCCg              >  2:211162/1‑251 (MQ=255)                                                                                                                                                                                                                                         cACGGGCGGCACGTTAAGTGCATTTATAAGTACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACCCCAATCAACCCCGGCAGAATAAAGCGGTGGTTGATGACGAAGCGACCGAGGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGGCGGCAGGATGTAATAACCGGAGCAAGCCGGTGCTGAAGCTACGAGGTAGGCCGGATCAACGCCGAt            <  2:462341/251‑1 (MQ=255)                                                                                                                                                                                                                                         cACGGGCGGCACGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCTCTGCCAGATCGCTCGGATAAGTCGGCAGGATGTAATAACCGTAGCAAGCCGGTGCTGGAGCCTCGATGTATGCCTGATCAACGCCGAt            <  1:580503/251‑1 (MQ=255)                                                                                                                                                                                                                                                   aCGCTAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAGGATGTAATAACCGTAGCAAGCCTGTTCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGcgcg  <  1:420559/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       AAGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATCGCTGCCAGATCGCTCGGATAAGTCGGCAGGATGTAATAACCGTAGCAAGCCGGTGCTGAAGCCACGATGTATGCCGGATCAACGCCGATCGCCAGCGCG  >  NC_000913/4347149‑4347641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑