Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,775,096	T→A	Y225N (TAC→AAC)	mtlD →	mannitol‑1‑phosphate 5‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,775,096	0	T	A	100.0%	310.9 / NA	91	Y225N (TAC→AAC)	mtlD	mannitol‑1‑phosphate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (46/45);  total (46/45)

AAGCGTGGGTAGAAGAACACGTTGGCTTTGTCGATTCCGCCGTTGACCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCG  >  NC_000913/3774851‑3775340                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     aaGCGTGGGTAGAAGAACACGTTGGCTTTGTCGATTCCGCCGTTGACCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTc                                                                                                                                                                                                                                                 <  1:572078/251‑1 (MQ=255)       gggTAGAAGAACACGTTGGCTTTGTCGATTCCGCCGTTGACCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGaaaa                                                                                                                                                                                                                                           <  2:408289/251‑1 (MQ=255)         gTAGAAGAACACGTTGGCTTTGTCGATTCCGCCGTTGACCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGGCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACt                                                                                                                                                                                                                                         <  2:119885/251‑1 (MQ=255)             aagaaCACGTTGGCTTTGTCGATTCCGCCGTTGACCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGcc                                                                                                                                                                                                                                     >  1:410837/1‑251 (MQ=255)             aagaaCACGTTGGCTTTGGCGATTCCGCCGTTGACCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCGCGGAAAACTGGcc                                                                                                                                                                                                                                     <  2:62429/251‑1 (MQ=255)                aaCACGTTGGCTTTGTCGATTCCGCCGTTGACCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGCAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGt                                                                                                                                                                                                                                  >  1:412558/1‑251 (MQ=255)                aaCACGTTGGCTTTGTCGATTCCGCCGTTGACCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGCCAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGt                                                                                                                                                                                                                                  >  2:522085/1‑251 (MQ=255)                    cGTTGGCTTTGTCGATTCCGCCGTTGACCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGCAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGtcatc                                                                                                                                                                                                                              >  2:40554/1‑251 (MQ=255)                     gTTGGCTTTGTCGATTCCGCCGTTGACCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGtcatc                                                                                                                                                                                                                              >  2:621677/1‑250 (MQ=255)                      ttGGCTTTGTCGATTCCGCCGTTGAACGCATAGTAACGCCTTCGGCTTCCGGAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTAAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGTGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACACTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGccatca                                                                                                                                                                                                                             >  2:423128/1‑250 (MQ=255)                        ggCTTTGTCGATTCCGCCGTTGACCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGa                                                                                                                                                                                                                           <  1:40554/250‑1 (MQ=255)                          cTTTGTCGATTCCGCCGTTGCCCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACATGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGCAAACTGGCCGGTCATCAGAcc                                                                                                                                                                                                                         >  1:369781/1‑250 (MQ=255)                              ggcGATTCCGCCGTTGACCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATCGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTc                                                                                                                                                                                                                     <  2:149568/248‑1 (MQ=255)                              gTCGATTCCGCCGTTGACCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGTCCGGTCATCAGACCATTct                                                                                                                                                                                                                    >  1:271456/1‑250 (MQ=255)                              gTCGATTCCGCCGTTGACCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTct                                                                                                                                                                                                                    <  1:490919/251‑2 (MQ=255)                                 gATTCCGCCGTTGACCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTcttg                                                                                                                                                                                                                  >  1:466254/1‑247 (MQ=255)                                               ccGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTcgacg                                                                                                                                                                                                   <  1:38033/251‑1 (MQ=255)                                                     cGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGaaa                                                                                                                                                                                              >  2:92709/1‑250 (MQ=255)                                                       tACCGCCTTCGACTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGCGAAAATc                                                                                                                                                                                           >  2:611339/1‑251 (MQ=255)                                                        aCCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATcc                                                                                                                                                                                          >  1:325874/1‑251 (MQ=255)                                                         ccaccTTCGGCTTCGGCACCTAACGATCCGCTGGAAGTGACGGTACGAACCTCCAGCGAATGAATTGTCGATAAAACGCAGTTCACCGGCGCACTGCCGACCATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCCACCGTAACCTCTTCACACTGACCACGGGTCATGCTACCACCGCGACCCTCGGCAAACTGGCCGGTCATCAGACCATTCTTGCCGCGATTCTCGCCGAGACAAtacg                                                                                                                                                                                         >  2:345467/4‑248 (MQ=255)                                                           gCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCcgcg                                                                                                                                                                                       <  1:214892/251‑1 (MQ=255)                                                             cttcggcttcggCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGTACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCcgcgcg                                                                                                                                                                                     >  2:189967/1‑251 (MQ=255)                                                             cttcggcttcgcAAACTAAAGATCCGCTGGGCGTGACGGTAGAAACCTTCAGCGAATGGATTGTAGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAACCTGGCCGGTCATCCGACCATTCTTGCCGCGATTCTCGACGAGAAAATCcgcgcg                                                                                                                                                                                     >  2:607559/1‑251 (MQ=255)                                                                 ggcttcggcAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCggtgg                                                                                                                                                                                 <  1:355522/251‑1 (MQ=255)                                                                  gcttcggcAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTTACGCGATTCTCGACGAGAAAATCCGCGCggtggt                                                                                                                                                                                <  2:662342/251‑1 (MQ=255)                                                                  gcttcggcAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCggtggt                                                                                                                                                                                >  2:274611/1‑251 (MQ=255)                                                                         cAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTAAGAGAATGGAATGTCGATAAAACGCAGATCAAAGGCGCACTGAAGACCATCCCAGGCATGGAGTTAAACGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGAGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCATGACGCGATTCTCGACGAGACAATCCGCGCGGAGGTaatatgg                                                                                                                                                                         >  2:691439/1‑246 (MQ=255)                                                                           aCTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGc                                                                                                                                                                       >  1:607782/1‑251 (MQ=255)                                                                                cGATCCGCTGGAAGTGACGGTAGAACCCTCCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCGCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATgg                                                                                                                                                                  <  2:380502/251‑1 (MQ=255)                                                                                 gATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGga                                                                                                                                                                 <  1:425208/251‑1 (MQ=255)                                                                                    ccGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGga                                                                                                                                                                 >  1:394870/1‑248 (MQ=255)                                                                                    ccGCTGGAAGTGACGGTAGAAACCTTCAGCGAAGGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGgaag                                                                                                                                                               <  1:583633/250‑1 (MQ=255)                                                                                     cGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCGCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGtaagaa                                                                                                                                                             <  2:691627/251‑1 (MQ=255)                                                                                     cGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGACGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGgaagaa                                                                                                                                                             <  2:5128/251‑1 (MQ=255)                                                                                        tGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAGCCTGATGGCATTTGTCGAACGTAAACTCTTCTCCCTGAACACGGGTCATGCTATAACCGCGAACCt                                                                                                                                                                                                                                                  <  2:439235/163‑1 (MQ=255)                                                                                        tGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCt                                                                                                                                                                                                                                                  >  1:439235/1‑163 (MQ=255)                                                                                         ggAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAGAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAgtg                                                                                                                                                         >  1:69276/1‑251 (MQ=255)                                                                                         ggAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTTACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCAGCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAgt                                                                                                                                                          <  2:638221/250‑1 (MQ=255)                                                                                         ggAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAgtg                                                                                                                                                         <  2:607782/251‑1 (MQ=255)                                                                                           aaGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAAAACATCCGCGCGGTGGTAAAAGGAGCGATGGAAGAAAgtggt                                                                                                                                                       >  1:708383/1‑251 (MQ=255)                                                                                                     aGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACTCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCTCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGa                                                                                                                                             <  1:227850/251‑1 (MQ=255)                                                                                                         aCCTTCAGCGAAGGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCAGTTTTCGAACGTAAACCCTTCACCCTGAACGCTGGTCATGCGATAACCGCGAACCTCGGAAAACTCGCCGGTCGTCAGACCATTGTTGACGCGATTCTCGACGAGAAAATCCGGGCGGTGGTAAAAGGTGCGGTGGGAGAGGCTCGTGCGGTATTGATCaa                                                                                                                                         <  2:72948/251‑1 (MQ=255)                                                                                                          ccTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGGCCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGTTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAg                                                                                                                                        <  1:560338/251‑1 (MQ=255)                                                                                                             tCAGCGAATGGATTGTCGATAAAACGCAGTTTAAAGGCTCACTGCAGAACCTCCCGGGCATGTAGTTAACCTCCAACCGTATGGCATTTGTGTAACGTAAACTCGTCACCCGGAACGCGTGTCAGGCTATAACCGCGAGCCTCGGAAAACGGGCCGGTCAGCAGACCAGTCGTGACGCGAGTCTCGAGGAGAAAATCCGCGCTTTGGTAAAAGGTGGGTTGGAAGAAAGTGTTGCAGTTTGGATCAAgc                                                                                                                                       <  2:322804/249‑1 (MQ=255)                                                                                                              cAGCGACTGGATGGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTATTCACCCTGACCACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGACGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAATTGGTGCAGTATTGATCAAGCGCt                                                                                                                                    >  1:660151/1‑251 (MQ=255)                                                                                                                 cGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACg                                                                                                                                 <  2:53623/251‑1 (MQ=255)                                                                                                                     tGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGCCGAGAAAATCCGCGCGGTGGTAAACGGTGCGATGGAAGAAAgtggtg                                                                                                                                                      >  2:643595/1‑226 (MQ=255)                                                                                                                     tGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAgtggtg                                                                                                                                                      <  1:643595/226‑1 (MQ=255)                                                                                                                       gATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTg                                                                                                                           >  1:277910/1‑251 (MQ=255)                                                                                                                          tGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGAc                                                                                                                         <  1:606148/250‑1 (MQ=255)                                                                                                                                 aaaaCGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACa                                                                                                                  <  1:626434/250‑1 (MQ=255)                                                                                                                                   aaCGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGc                                                                                                               >  2:560768/1‑251 (MQ=255)                                                                                                                                      gCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATg                                                                                                            >  1:178445/1‑251 (MQ=255)                                                                                                                                      gCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATg                                                                                                            <  1:416678/251‑1 (MQ=255)                                                                                                                                       cAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTCAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGCAAAAGGAGCGATGGAAGAAAGTGGTGCAGAATTGATCAAGCGCTACGGCTTTGACGCTGAAAAGCacg                                                                                                            >  2:603600/1‑248 (MQ=255)                                                                                                                                        aGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATgcg                                                                                                          <  1:514615/251‑1 (MQ=255)                                                                                                                                        aGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATgcg                                                                                                          >  1:474879/1‑251 (MQ=255)                                                                                                                                         gTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATgcgg                                                                                                         <  1:704120/251‑1 (MQ=255)                                                                                                                                              aaGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTAc                                                                                                    <  2:176361/251‑1 (MQ=255)                                                                                                                                                ggCGCTCTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGTTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACAt                                                                                                  <  2:517451/251‑1 (MQ=255)                                                                                                                                                  cgcACTGCCGCACATCACCGGCATGGAGTTAACCGACCACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACAGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGCCAAGCATGCGGCGTACATcc                                                                                                >  2:505013/1‑251 (MQ=255)                                                                                                                                                   gcACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCa                                                                                               <  1:447935/251‑1 (MQ=255)                                                                                                                                                     aCTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATcc                                                                                                >  2:246460/1‑248 (MQ=255)                                                                                                                                                       tGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGAt                                                                                                                                                                                                           >  1:378214/1‑139 (MQ=255)                                                                                                                                                       tGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCGGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGAt                                                                                                                                                                                                           <  2:378214/139‑1 (MQ=255)                                                                                                                                                          cGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCAGTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAAtt                                                                                        <  2:581879/251‑1 (MQ=255)                                                                                                                                                                      gCATGGATTTAGCCGACAGCCTGATGGCATTTGTCGAACGTAAACTCGTCACCCTGAACTCGGGTCATGCTATATCCGCGAACCTCGGAAAACTGTCCGGTGATCAGCCCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATTGAAGAAAGTGGTGCATTATTGATCAAGCGCTACGGCTATGACGCTGACAAGCATGCGGCGTACACCCAGAAAATTCTCGGCCGtttt                                                                            <  1:415028/251‑1 (MQ=255)                                                                                                                                                                        aTGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTga                                                                          <  1:178980/251‑1 (MQ=255)                                                                                                                                                                          ggAGTTAACCGACAACCTGATGTCATGTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCAGGCTATAACCGCGATCCCCGGAAAACGGGGCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTgaga                                                                        <  1:345467/251‑1 (MQ=255)                                                                                                                                                                             gTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAAcc                                                                     <  2:394870/251‑1 (MQ=255)                                                                                                                                                                              ttAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAAccc                                                                    >  1:605543/1‑251 (MQ=255)                                                                                                                                                                               tAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAAccc                                                                    <  2:605543/250‑1 (MQ=255)                                                                                                                                                                                aaCCGACAACCTGATGGCCTTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAAACTCGGAAAACTGGCCGGTCATCAGACCCTTCTTGACGCGATTCTCGACGAGAAAATCCGAGCGGTGGTAAAAGGTGCGATGGAAGAAAGCGGGGCAGCATTGCTCCAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACAACCAGAAAATTCTCGGCCGATTTAAGAACCCGt                                                                  >  2:30927/1‑251 (MQ=255)                                                                                                                                                                                aaCCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGt                                                                  >  1:452546/1‑251 (MQ=255)                                                                                                                                                                                       aaCCTGATGGTAGTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAATGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGTCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGaa                                                           <  1:607559/251‑1 (MQ=255)                                                                                                                                                                                        aCCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAACAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGaa                                                           >  1:430706/1‑250 (MQ=255)                                                                                                                                                                                         ccTGATGGCCTTTGTCGAACGTAAACTCTTCACCCTGAACCCGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAAACCGTATCTGaa                                                           >  2:405067/1‑249 (MQ=255)                                                                                                                                                                                                gCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGt                                                  <  1:558123/251‑1 (MQ=255)                                                                                                                                                                                                  aTTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGCCGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGAGCGCTGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCt                                                                                                                      >  1:703440/1‑181 (MQ=255)                                                                                                                                                                                                  aTTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGt                                                  >  2:296390/1‑249 (MQ=255)                                                                                                                                                                                                   tttGTCGCAAGTAAACTCCTCAACCTGAAAACGGGTCATGCTATAACCGCGAACCTCGGAAAAATGGCCGGTCATCAGACCATTCTTCACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGCAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGt                                                  >  2:465379/1‑248 (MQ=255)                                                                                                                                                                                                     tGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTagag                                              <  2:491227/250‑1 (MQ=255)                                                                                                                                                                                                      gTCGAACGTAAACCCTTCACCCGGAACACGGGTCATGCGATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAgc                                             <  2:412558/250‑1 (MQ=255)                                                                                                                                                                                                                  ctctTCACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTc                                 >  2:541396/1‑250 (MQ=255)                                                                                                                                                                                                                       cACCCTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTATTCCGTCAGCCAc                           <  1:189967/251‑1 (MQ=255)                                                                                                                                                                                                                          ccTGAACACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGc                        >  1:231412/1‑251 (MQ=255)                                                                                                                                                                                                                              aaCACGGGTCATGCTATAACCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTa                     >  2:118191/1‑250 (MQ=255)                                                                                                                                                                                                                                               aaCCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGAcc   >  1:251886/1‑251 (MQ=255)                                                                                                                                                                                                                                                aCCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGCTTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCg  >  2:149128/1‑251 (MQ=255)                                                                                                                                                                                                                                                aCCGCGAACCTCGGAAAACTGGCCGGTCATCAGACCATTCTTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCg  >  1:76201/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     AAGCGTGGGTAGAAGAACACGTTGGCTTTGTCGATTCCGCCGTTGACCGCATCGTACCGCCTTCGGCTTCGGCAACTAACGATCCGCTGGAAGTGACGGTAGAAACCTTCAGCGAATGGATTGTCGATAAAACGCAGTTCAAAGGCGCACTGCCGAACATCCCAGGCATGGAGTTAACCGACAACCTGATGGCATTTGTCGAACGTAAACTCTTCACCCTGAACACGGGTCATGCTATAACCGCGTACCTCGGAAAACTGGCCGGTCATCAGACCATTCGTGACGCGATTCTCGACGAGAAAATCCGCGCGGTGGTAAAAGGTGCGATGGAAGAAAGTGGTGCAGTATTGATCAAGCGCTACGGCTTTGACGCTGACAAGCATGCGGCGTACATCCAGAAAATTCTCGGCCGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCG  >  NC_000913/3774851‑3775340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑