Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,205	C→A	T29K (ACG→AAG)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,205	0	C	A	100.0%	182.4 / NA	53	T29K (ACG→AAG)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/22);  total (31/22)

CATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAC  >  NC_000913/3485968‑3486449                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     cATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGTTTCTTGTCTCATTGCCACATTCATAAGTCCCCATCCAAGCGCAAGCTTATTCAcc                                                                                                                                                                                                                                           >  2:540668/1‑249 (MQ=255)          ccTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTTCAGTTGATAGCCCCTTCCCAGGGAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGaaaa                                                                                                                                                                                                                                 <  2:147422/249‑1 (MQ=255)            tGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGc                                                                                                                                                                                                                              >  2:490686/1‑251 (MQ=255)               aTGTATCCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATACCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTTCCCATCCAAGAGCAAGCTTATTCACCAGGTTGAAAAAGCGGa                                                                                                                                                                                                                           <  1:404832/251‑1 (MQ=255)                     gCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCt                                                                                                                                                                                                                     <  2:398018/251‑1 (MQ=255)                              gTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACAt                                                                                                                                                                                                            >  2:518859/1‑251 (MQ=255)                              gTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGAAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACAAGGGTGAAAAAGCGGAAAAGCTGTACTACAt                                                                                                                                                                                                            >  2:209215/1‑251 (MQ=255)                                cacaTTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAATCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATc                                                                                                                                                                                                           <  2:574218/250‑1 (MQ=255)                                  caTTACCGTGCAGGACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGtt                                                                                                                                                                                                        <  1:645681/251‑1 (MQ=255)                                             aGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgt                                                                                                                                                                                             <  1:150864/251‑1 (MQ=255)                                                 cAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCa                                                                                                                                                                                         <  2:553746/251‑1 (MQ=255)                                                   gTTGATAGACCCTGCCCAGGAAGCGGGAAGCATATGACGGAAAACCAGAGACAGCGGCTTAATCTGGCGCGGGAGAAAGCTTATAACAAAGGATAAACGCGCAAGGTTCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGATCTGGTCACATAGCCACAGTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCAGTACTACATCGTTAAAGGCTCTGGGGCAGt                                                                                                                                                                                       <  2:642964/251‑1 (MQ=255)                                                      taTAGCCCCTTCCCAGGTAGCGGGAAGCATAGTTCGGCAATCCAGAGACAGCGGCTTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAATCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCGTGTCTCAGTGCCACATTCAGAAGTACCCATCCAAGATCAAGCTTATTCACCAGGTTGAAAAATCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGc                                                                                                                                                                                     <  1:471040/249‑1 (MQ=255)                                                      gATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCt                                                                                                                                                                                    >  1:61832/1‑251 (MQ=255)                                                       aTAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGCGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTATCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTg                                                                                                                                                                                   >  1:38411/1‑251 (MQ=255)                                                                         gCGGGAAGCAGATTTCGGCAATCCAGCGACAGCGGCGTTATCTGGCTCTGGGGAAAGCCTATAACAGAGGATAACCGCGCATGGTGCTGGGCAAACCGCAAACAGTCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCGGATCAAAGACGAAGAggtt                                                                                                                                                                 <  2:146614/251‑3 (MQ=255)                                                                                             aTCCAGAGACAGCGGCGTTATCTGGATCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAACCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGATGTACTACATCGTTAAAGGATCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCtctcat                                                                                                                                              >  2:430868/1‑248 (MQ=255)                                                                                                 agagACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTg                                                                                                                                         >  1:307737/1‑251 (MQ=255)                                                                                                 agagACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTg                                                                                                                                         >  1:286830/1‑251 (MQ=255)                                                                                                    gaCAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAAt                                                                                                                                      >  1:530509/1‑251 (MQ=255)                                                                                                        gcgtcgTTATCTGGCTTTGGAGAAAGCCTACAACAGAGGATACCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATGCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGTTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCCCTCCTATCTGAATCAgt                                                                                                                                  <  2:5184/247‑2 (MQ=255)                                                                                                             gTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATCGCGCTTGGCAAACCGCAAAAAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGCACCCATCCAAGAGCAAGCTTATTAACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGAGGCCGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAt                                                                                                                             >  1:329518/1‑251 (MQ=255)                                                                                                              ttatcagaatctggaaaaagcgccaaaCAGAGGCTAACATCGCATGGAGCTTGGCAAAACGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACCTTCATAACTACCCATCCAAGATCAAGCATCTTCACCAGCCTGAACAAGCGGAAACACTGGACTACATCGTTAAAGGCTCTGTGGCATTGCTGATCACAGACGTAAAGGGAAAAGAAATGATCCTCTCCTATCTGTATATGGGTGAtt                                                                                                                            >  2:461146/26‑251 (MQ=255)                                                                                                                   tGGCTCTGGAGAAAGCTTATAACAGAGGATAATCGCGCATGGTGCTTGGCAAACCGCAAAAAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTAtt                                                                                                                       >  2:129131/1‑251 (MQ=255)                                                                                                                    ggCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTg                                                                                                                      <  1:534653/251‑1 (MQ=255)                                                                                                                      ctctGGAGAAAGCTTATAACAGAGGATAACCGCGAATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGATCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGc                                                                                                                    >  1:632497/1‑251 (MQ=255)                                                                                                                            agaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAAAAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGCTCAAAGACGCAGAGGGTAACGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATGGGCGAACTg                                                                                                              >  1:180449/1‑251 (MQ=255)                                                                                                                             gaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGTTGATTTTATTGGCGAACTgg                                                                                                             <  1:540668/251‑1 (MQ=255)                                                                                                                                           gagGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGt                                                                                                                                                                                                                   <  1:427663/135‑1 (MQ=255)                                                                                                                                           gagGATAAACGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGt                                                                                                                                                                                                                   >  2:427663/1‑135 (MQ=255)                                                                                                                                            agGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAgg                                                                                              <  2:530509/251‑1 (MQ=255)                                                                                                                                                aatccccccATGGTGCTAGCCAAACCGCAATCACACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGCACCCAGCCAAGCGCAAGCTTATGCTCCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCCTTGCTGATCAAAGACGAAGTGGGTAAAGAAATGATCCTTTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGTGCCTGTTTGAAGAGGGCCa                                                                                          <  2:632497/243‑1 (MQ=255)                                                                                                                                                 aaCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAg                                                                                         <  1:192391/251‑1 (MQ=255)                                                                                                                                                  aCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAgg                                                                                        >  1:397140/1‑251 (MQ=255)                                                                                                                                                    cgcgCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCCCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCAATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGaa                                                                                      <  2:462/251‑1 (MQ=255)                                                                                                                                                     gcgcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAAc                                                                                     >  2:202919/1‑251 (MQ=255)                                                                                                                                                      cgcATGGTGCTTGGCAAACCGCAAACAGACACGACTCTCGACTGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGAACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGAGCGAGGAACg                                                                                    >  1:300332/1‑251 (MQ=255)                                                                                                                                                         aTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGAGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGt                                                                                   >  2:282001/1‑249 (MQ=255)                                                                                                                                                         aTGGTGCTTGACAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCTAGCTTATTCACCAGGGTGAAAAAGCGGCAACGCAGTACTACATCGTTAAAGGCTCTGTGGCAGGGCTGATCAAAGACGACGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCAAGGAACGt                                                                                   >  2:549988/1‑249 (MQ=255)                                                                                                                                                              gCTTGTCAAACCGCAAACGTACCCGACTCTCGAAGGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCTGAACCGCTGTACTACAGCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGTGTAAAGAAATGATTCTCGCCGATCTGAATCAGGGTGGGTGTATTGGCGAGCTGGGCCTGTTTGAATATGGCCAGGGACGAAGCGCAt                                                                            <  2:292857/251‑1 (MQ=255)                                                                                                                                                               cTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTg                                                                                                                      >  2:136024/1‑208 (MQ=255)                                                                                                                                                               cTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTg                                                                                                                      <  1:136024/208‑1 (MQ=255)                                                                                                                                                                            aaaCAGACCCGACTATCGAATGGCTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGaa                                                               >  2:101522/1‑250 (MQ=255)                                                                                                                                                                             aaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGaaaa                                                             <  1:229598/251‑1 (MQ=255)                                                                                                                                                                                  aCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGcc                                                        <  1:518859/251‑1 (MQ=255)                                                                                                                                                                                            cGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTgg                                              >  2:372809/1‑251 (MQ=255)                                                                                                                                                                                                tGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTg                                           >  2:560880/1‑250 (MQ=255)                                                                                                                                                                                                tGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGAGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTg                                           >  1:219805/1‑250 (MQ=255)                                                                                                                                                                                                 ggTTCTTGTCTCATTGCAACATTCATAAGTAACCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGCCGAAGAGGGTAAAGACATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGa                                          >  1:253328/1‑250 (MQ=255)                                                                                                                                                                                                           tcATTGCCAAATTCATAAGTACCCATCCAAGCGCAAGCTTATTCACCAGGGTGAAAAAGCGGACACGCTGTACTACATCGTTAAAGGATCTGTGGCAGTGCTGATCACAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGAGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGAGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTAc                                >  2:425004/1‑250 (MQ=255)                                                                                                                                                                                                                      ttCATAAGTAACCATCCACGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTCAATAGTTAAAGGATCTGTGGCAGTGCAGATCAAAGACGAAGAGGGTAAAGAAATGATCATCTCATATCTGAATCCGGGTGCTTTTATTGGCGAACTGGGCCTGTCTGAAGAGGGCCAGAAACGTAGCGCATGGGTACGTGCGACAACCGCCTGGGAAGTGGCTGAAATTTCGTAAAAACAATTTCGc                    >  2:607471/1‑251 (MQ=255)                                                                                                                                                                                                                                 ccATCCAAGAGCAAGCTTATTCACCAGGGTGAACAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTc          >  2:202545/1‑250 (MQ=255)                                                                                                                                                                                                                                        agagCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAc  >  1:176273/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     CATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAC  >  NC_000913/3485968‑3486449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑