Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,942,689	T→C	intergenic (‑122/‑7)	gcvA ← / → gcvB	DNA‑binding transcriptional dual regulator GcvA/small regulatory RNA GcvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,942,689	0	T	C	100.0%	128.5 / NA	38	intergenic (‑122/‑7)	gcvA/gcvB	DNA‑binding transcriptional dual regulator GcvA/small regulatory RNA GcvB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/16);  total (22/16)

GATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCG  >  NC_000913/2942445‑2942915                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   gatttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTCTTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATCCCTATAGCggcggc                                                                                                                                                                                                                              >  2:587985/1‑251 (MQ=255)  atttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCggcggc                                                                                                                                                                                                                              >  1:674911/1‑250 (MQ=255)  atttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGAGCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAgcggcgtca                                                                                                                                                                                                                             <  2:28311/251‑4 (MQ=255)     tgatgaCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACtt                                                                                                                                                                                                                          >  2:177597/1‑251 (MQ=255)      gatgaCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTc                                                                                                                                                                                                                         <  1:123688/251‑1 (MQ=255)      gatgaCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTCCGCGAGTTAAACTTAAATGGCGTGCTGCTGCATCAAAAACTCGTAAGGCATGTAGCGGTGGTAATCGTGTAGACATGGCTATTAAACTTTGATGTTAAATGAATTGAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTc                                                                                                                                                                                                                         <  1:244956/251‑1 (MQ=255)                  cGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACGCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTGTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAAc                                                                                                                                                                                                             <  1:590987/251‑1 (MQ=255)                  cGGCTTGGGTCACAAAAAGCTCTTCGGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGGTTTTTGAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAAc                                                                                                                                                                                                             <  1:99978/251‑1 (MQ=255)                      ttGGGTCACAAAAAGCTCTTCTGCTGCGCGAGAGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTTATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGaccgga                                                                                                                                                                                                          >  1:105885/1‑245 (MQ=255)                                aaaaGCCCTTCTGCTGCGCGAGTGAATCTTACATGGCGTGCTGCGGCATAAAAACCTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACCTGGCTATTAAACTTTGATGTTACATGAATTTACCACTTAGCTCACACTATGTAACCTATTCGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAACTCACTATAGCGGCGGCCCTTACTGAGCCGGAACTCAAAGTTTTATCg                                                                                                                                                                                               >  2:289996/1‑251 (MQ=255)                                         tctGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTGTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCgt                                                                                                                                                                                       <  1:73451/250‑1 (MQ=255)                                              tgcGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTATACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTg                                                                                                                                                                                 >  1:29449/1‑251 (MQ=255)                                                    aGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAAc                                                                                                                                                                           >  1:11148/1‑251 (MQ=255)                                                              aaaTGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCtt                                                                                                                                                                  >  2:377020/1‑250 (MQ=255)                                                                    cGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTtg                                                                                                                                                           >  1:338792/1‑251 (MQ=255)                                                                      tgctgcGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACCATTAGATCACACTATGTAACCTATTCGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTtgtg                                                                                                                                                         >  1:68648/1‑251 (MQ=255)                                                                           cGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGAtgtt                                                                                                                                                    >  1:249347/1‑251 (MQ=255)                                                                               aTCAAAAACTCGTAAGGCCTTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGAtgttgtgt                                                                                                                                                >  1:487467/1‑251 (MQ=255)                                                                                  aacaaCTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTGGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGGTTTTTTAATCTGAGCCATGATAAATTGTCCGTTGATCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTGCTGTTGAACTTTTGGCTTACGATTGCGAtgttgtggtgt                                                                                                                                             <  2:528140/248‑1 (MQ=255)                                                                                                         gtggtAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGAt                                                                                                                      >  1:402567/1‑251 (MQ=255)                                                                                                                         cATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCa                                                                                                      <  1:177597/251‑1 (MQ=255)                                                                                                                          aTGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGAAACCTCTTAATTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGATTCTACAAGCAAATACCTATAGCAGCAGCACTTCCTGAGCCGGAACGAAAAGATTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTAACGGTTGTGATGTTGTGTTGTTGGGTTTGCAATTGGTCTACTATTCAGACAATGGTAGCa                                                                                                      >  2:218113/1‑250 (MQ=255)                                                                                                                             gCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGc                                                                                                  <  1:550491/251‑1 (MQ=255)                                                                                                                              cTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCt                                                                                                 >  1:557145/1‑251 (MQ=255)                                                                                                                                aTTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGGCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAAt                                                                                                                                                                                           <  1:668832/159‑1 (MQ=255)                                                                                                                                        ttGATGTTAAATGACTTTAACAATTAGATCACACTATGTAACGTATTAGTTTTTTTATTCTGCGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCCAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAACGCTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGTTTCAGACCATTGTAGCCAAGCTACCTTTTTTc                                                                                       >  2:288800/1‑251 (MQ=255)                                                                                                                                                         tAACAATTAGAGCACACTATGTAACATATTAGTTTTTTTCATCTGAGCCCTTATAAATTGCACGTAGAACTTCAACCAGCAAATCCATAAAGCGGCGGCACTTAATGCGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGCGCCATATTGGATTAAGGATGTGATGTTGTGTTGTTGGGTTTGCAATTGGTCTGCGATTAAGACCATGGTAGCAAAGCTACCTTTTTTAACTTCATGTAAATTTAc                                                                      >  2:694041/1‑251 (MQ=255)                                                                                                                                                          aaCAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTAcc                                                                     <  2:532757/251‑1 (MQ=255)                                                                                                                                                                  gATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGCTTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCctgtctgt                                                             >  1:236529/1‑251 (MQ=255)                                                                                                                                                                   aTCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCtgtctgtc                                                            >  1:38369/1‑251 (MQ=255)                                                                                                                                                                       cacTATGTCACCTATTAGTTTATTTAATCTGAGACATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGGAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGATGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCACAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCAtt                                                        >  2:599772/1‑250 (MQ=255)                                                                                                                                                                                            ttttAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATGGTGATTAATGTAGCACCGCCt                                   >  1:405723/1‑251 (MQ=255)                                                                                                                                                                                              ttAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTa                                  <  1:434728/250‑1 (MQ=255)                                                                                                                                                                                                aaTCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAAtt                               <  1:100514/251‑1 (MQ=255)                                                                                                                                                                                                                 aTTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCGGTACATTTACCCTGGCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTAc              <  1:301686/251‑1 (MQ=255)                                                                                                                                                                                                                    gTCCGTTGAGCTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAAGGCGTTTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATTGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTGCCCTGTCTGTCCATAGGGATTAATGTAGCAGCGCCTAATTGCGGTGCGTTTTTTTACCtt           <  2:366092/251‑1 (MQ=255)                                                                                                                                                                                                                       cGTTGAGCTTCTACCAGCAAATTCCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCg        <  1:570975/251‑1 (MQ=255)                                                                                                                                                                                                                           gAGCTTCGACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATTGTAGCAAAGCTACCGTTTTTCACTTCCTGTACATTTACCCTGTCTTTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATc     <  2:218626/250‑1 (MQ=255)                                                                                                                                                                                                                              cTTCTACCAGCAAATACCTATAGCGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCCAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTCATTGCGGCGCTTTTTTTTACCTTGCGATcgcg  >  1:675051/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                   GATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCG  >  NC_000913/2942445‑2942915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑