Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,488,630	A→T	L180* (TTA→TAA)	yfdE ←	acetyl‑CoA:oxalate CoA‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,488,630	0	A	T	100.0%	187.4 / NA	52	L180* (TTA→TAA)	yfdE	acetyl‑CoA:oxalate CoA‑transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (25/27);  total (25/27)

GCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAG  >  NC_000913/2488387‑2488844                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                       gCAGAAAAAAGCTTGTCATTACCACAACAAATCTTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTTAATTAATAGac                                                                                                                                                                                                                 <  1:103757/251‑1 (MQ=255)     aaaaaaGCTTCTCATTACCACAACAAATCGTAATCAGCATATCCTAAGTCTTGAAAACATCAAAAGGAGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCCCTATTCCGATGATTTAATAGACAccg                                                                                                                                                                                                             >  2:299497/1‑251 (MQ=255)                  aTTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAACGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAAATCCg                                                                                                                                                                                                >  2:200301/1‑251 (MQ=255)                      ccACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCa                                                                                                                                                                                              >  1:146650/1‑249 (MQ=255)                        acaacaAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGAGTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAagag                                                                                                                                                                                          <  2:273144/251‑1 (MQ=255)                          aacaaATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAag                                                                                                                                                                                            >  2:194007/1‑247 (MQ=255)                              aaTCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTAc                                                                                                                                                                                    >  1:406760/1‑251 (MQ=255)                               aTCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCTGCATAGATCCGCAAGAGATGTAcc                                                                                                                                                                                   >  1:396115/1‑251 (MQ=255)                                  gTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAAc                                                                                                                                                                                <  1:226565/251‑1 (MQ=255)                                        ggCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCAc                                                                                                                                                                          <  1:206703/251‑1 (MQ=255)                                         gCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCg                                                                                                                                                                                                >  1:310901/1‑228 (MQ=255)                                         gCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCg                                                                                                                                                                                                <  2:310901/228‑1 (MQ=255)                                                cTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCgg                                                                                                                                                                    >  1:172861/1‑249 (MQ=255)                                                    gTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATc                                                                                                                                                              >  2:283301/1‑251 (MQ=255)                                                           aaaCATCAAAAGGGGCCATGTAGGGATGGCGATTTCAAAGACGTTGCGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATCTCGACATGCGCCCCTCTCTGGCTCTTTTCGCGTCCATAAAGTGCACTCACTATTCCGCTGAATTACTAAACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGCAGCATCAGGctat                                                                                                                                                       >  2:638926/1‑251 (MQ=255)                                                               aTCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGTCCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACTAACGCGCACTGGCGGAGAATCAGGATATCCTg                                                                                                                                                   <  1:537427/251‑1 (MQ=255)                                                               aTCAAAAGGTGCCATGGAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCAGCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCTCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCTCAAGAGATGTACCGACGGGCACTGGCGGAGCATCATGAGATCCTg                                                                                                                                                   <  2:638610/251‑1 (MQ=255)                                                                 cAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGt                                                                                                                                                  <  1:110345/250‑1 (MQ=255)                                                                            aTGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTac                                                                                                                                      <  2:492808/251‑2 (MQ=255)                                                                              gTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCGCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCCCTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATc                                                                                                                                     <  2:406760/250‑1 (MQ=255)                                                                                   gATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCCGCGTGGCATCAAACATCGCTATATCGACATGCGCCACTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCACGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCAATCATTATCCCGCTc                                                                                                                               >  2:574231/1‑251 (MQ=255)                                                                                   gATGGCGATTTCCCAGACGGTGTGGTGACTTCCCTGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATAATCCCGCTc                                                                                                                               >  2:127397/1‑251 (MQ=255)                                                                                    aTGGCGATTTCCAAGACGTTGCGGTGACTTCCTAGTTGCGCTATATGCAATCAGACCATGCTCCAGAAACCTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCACTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTc                                                                                                                               >  2:371513/1‑250 (MQ=255)                                                                                     tGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATGTGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCTCTATATCGACATGCTCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCaa                                                                                                                                                                                 <  1:375111/199‑1 (MQ=255)                                                                                       gCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTg                                                                                                                           <  1:501010/251‑1 (MQ=255)                                                                                          aTTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCt                                                                                                                        >  1:156190/1‑251 (MQ=255)                                                                                           tttCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTg                                                                                                                       >  1:651656/1‑251 (MQ=255)                                                                                             tCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGa                                                                                                                      >  2:248379/1‑250 (MQ=255)                                                                                                           gtgACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGt                                                                                                         >  1:691823/1‑249 (MQ=255)                                                                                                            agACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCACGGCCATAAAGTGCACTCACTAGTCCCCTGGATTAATCGACACCGCCGCATAGATCCGCAAGAGATGTACCAACACGCACTGGCGGAGCATCAGGGTATCCTGTTTCCATCATTATCCCGCTCATTGCCTGGATGATGGTATCGtc                                                                                                        >  1:375840/2‑248 (MQ=255)                                                                                                                        tGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCCTCtt                                                                                          >  2:662411/1‑251 (MQ=255)                                                                                                                            atatatGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATc                                                                                                                                                              <  1:571508/179‑1 (MQ=255)                                                                                                                            atatatGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATc                                                                                                                                                              >  2:571508/1‑179 (MQ=255)                                                                                                                              atatGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCg                                                                                    <  1:662411/251‑1 (MQ=255)                                                                                                                                atGCCATCAGACACTGCTCCCGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTACTAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTCGGCAGGAGCATCTTTTAGCgg                                                                                   >  2:409253/1‑250 (MQ=255)                                                                                                                                        aGACCATGCTCCATAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCTGTATg                                                                          <  2:421892/251‑1 (MQ=255)                                                                                                                                             aTGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGa                                                                     <  1:536153/251‑1 (MQ=255)                                                                                                                                              ggCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGaa                                                                    <  1:299497/250‑1 (MQ=255)                                                                                                                                               gCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCCCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTTTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCTGACCGGTATGTCCGaaa                                                                   <  1:127397/251‑1 (MQ=255)                                                                                                                                                  ccAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCt                                                                <  1:270172/251‑1 (MQ=255)                                                                                                                                                     gAAAACTCAGCGGGGCATCACACATCGCTATATCGACATGCGCCACTCTCTGGCTCTTTTCGCGGCCATCAAGAGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTAGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGTTATCGTAGGCAGGAGCATCTTTTAGCGGCCCGGTATGTCCGAACCCTGAc                                                             >  2:686116/1‑251 (MQ=255)                                                                                                                                                                     aTCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGTAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGa                                             <  1:240829/251‑1 (MQ=255)                                                                                                                                                                       cAAACAGCGGTATATCGACAAGCGCCCCTCTGCGGCTCTTTCCGCAGCCGTAAAGTGCCCTCACTATGCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAGCGCGCACTGGCGGAGCATCACGATATCCTGTGTCCATCATAATCCCGCGCATTGGTTGAATGGTTGTATCTTAGGCAGGAGGAACTTTTAGGGGACCGGTATGGCCGGAACCTTACGATGAAGCATATATGAgg                                           <  2:647804/251‑1 (MQ=255)                                                                                                                                                                                       aaCATGCGCCCCGCTCTGGCTCTTTACGCGGCCATAAAGTGCTCTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGATATGTACCAACGCGCACTGGCGGGGCATCAGGATATCCTGTTTCCATCACTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCtt                           <  2:529935/250‑1 (MQ=255)                                                                                                                                                                                              gcCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGAAGCGt                    <  2:335974/251‑1 (MQ=255)                                                                                                                                                                                              cccccTCTCTGGCTCTTTTCTCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGAAGCGt                    <  1:288858/250‑1 (MQ=255)                                                                                                                                                                                                      ctGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGAAGCGTTTccc               <  1:283301/248‑1 (MQ=255)                                                                                                                                                                                                             ttttCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGAAGCGTTTCCCATGAAAAccc     >  1:282161/1‑251 (MQ=255)                                                                                                                                                                                                               ttcgctgctcaaacgtcacctcacaATTCCGCAGAATTAATAGACACCGCCGAAAAGATCCGCACGAGATCTACCACCACGCACTGACAGGGAATCAACAGCTCATGTTTACATCATTATTCCGAACATTACCTGAATCATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTACGTCCGAAACCTGACGATGAAGCAAAGCTGAGGCCCAGGTTAACTTCTTGCAGCGTTTCCACTGAAAAccccc   >  2:668756/20‑250 (MQ=255)                                                                                                                                                                                                                 cgctgCCATAAAGTGCACCCACTATTCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATAGCCTGTTGCCATCATTATCCCGCTCATTGCCTGAAGGATGGTATCGTAGGCAGGAGCATCTTTTAGCTGACCGGTATGTCCGATGCCTGACGATGAAGCATATATGAGGCGCTGGTTGATTTCTTGAATCGTGTCCCATGAAATCCCCAg  <  2:651656/246‑1 (MQ=255)                                                                                                                                                                                                                                          tCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGt                                                                             <  2:352392/150‑1 (MQ=255)                                                                                                                                                                                                                                          tCCGCTGAATTAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGt                                                                             >  1:352392/1‑150 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                       GCAGAAAAAAGCTTGTCATTACCACAACAAATCGTAATCGGCTTATCCTGAGTATTGAAAACATCAAAAGGTGCCATGTAGGGATGGCGATTTCCCAGACGTTGTGGTGACTTCCCAGTTGCGATATATGCCATCAGACCATGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAG  >  NC_000913/2488387‑2488844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑