Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	414,799	G→A	R319C (CGC→TGC)	sbcC ←	ATP‑dependent structure‑specific DNA nuclease ‑ SbcC subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	414,799	0	G	A	100.0%	312.1 / NA	88	R319C (CGC→TGC)	sbcC	ATP‑dependent structure‑specific DNA nuclease ‑ SbcC subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (51/37);  total (51/37)

CGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAACCAGTTTAGCGATTGTTGTTCTTGCTGCTGCGCG  >  NC_000913/414554‑415043                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      cgcAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTAt                                                                                                                                                                                                                                                  <  1:135847/251‑1 (MQ=255)    aaGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTAttt                                                                                                                                                                                                                                                >  1:621017/1‑250 (MQ=255)      gcgcATTAAGTTTTTGCCTAGCATGGGTTACCTTTTGCCGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTTTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTAct                                                                                                                                                                                                                                             <  2:130767/251‑1 (MQ=255)       cgaaTTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTGTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTActt                                                                                                                                                                                                                                            <  1:281751/248‑1 (MQ=255)            taggtgtttGCCCAGCAGGGGTTATCTGTGCCTGCCATTGCCGCAGATGCTCGCGATCGCTGGGTTGTTGGGAGAACGGCGCGCGCCACCCCGCCGGTTCGTTGTTCTTCTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATGGTGCTCTGTAAGCAAGTATTTACttcttc                                                                                                                                                                                                                                        <  2:482303/244‑1 (MQ=255)               gTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATTCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGGGTTCCTGTAACCAGGTATTCAGGCTTTGTTTCTGCTGCTGTAATTCTGCTCACTGATTCGCCGCGTGGTGGCGAATGCTCGCGCGAGGCGCCATTGTGCTCTGTAAGCAAGTCTTTACTTCTTCGATc                                                                                                                                                                                                                                    >  2:5608/1‑251 (MQ=255)                 ttttGCTCAGCATGGTTTAACTGTTGCTGCCATGGCCGCAGATGCCCGGGATCGCGGGTTTGTTGGGAGAACGGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATct                                                                                                                                                                                                                                   <  1:8526/250‑1 (MQ=255)                 ttttGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTAct                                                                                                                                                                                                                                             <  1:211089/240‑1 (MQ=255)                 ttttGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTAct                                                                                                                                                                                                                                             >  2:211089/1‑240 (MQ=255)                    tGCTCAGCAGGGGTTAGCTGTTGCTGCAATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGACCTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATctgct                                                                                                                                                                                                                                <  1:634576/250‑1 (MQ=255)                     gCTCACCATGGGTTAACTGTCGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTgg                                                                                                                                                                                                                              >  2:360416/1‑251 (MQ=255)                      cTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGc                                                                                                                                                                                                                             >  2:179244/1‑251 (MQ=255)                        cAGCATGGGTTATCTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCACCCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGc                                                                                                                                                                                                                             <  1:123231/249‑1 (MQ=255)                        cAGCATGGGTTAACTGTTGCTGCCAGTGCCGCAGATGCTCGCGATCGCTGGATTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTACAATGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGATCGCGCGAAGCGCCATTGAGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCg                                                                                                                                                                                                                            >  1:259122/1‑250 (MQ=255)                              gggTTAAGTGTTGCTGCCAGTCCCGCGGATCCCCGCGATCGCTGTGTTGTTGGGAAAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCTGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATg                                                                                                                                                                                                                      <  2:600281/250‑1 (MQ=255)                              gggTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAGGTATTTACTTCTTCAATCTGATGGCGCATATgc                                                                                                                                                                                                                     >  1:402313/1‑251 (MQ=255)                              gggTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATgc                                                                                                                                                                                                                     >  2:304630/1‑251 (MQ=255)                                         tgctgcCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGcgccgc                                                                                                                                                                                                          >  1:132284/1‑251 (MQ=255)                                           ctgcCATTGCCGCAGCTGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTCTTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGCAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCgcgc                                                                                                                                                                                                        >  2:694927/1‑251 (MQ=255)                                             gcCATGGCCGCAAATGCTCGGTATCGGTGGTTTGTGGGGATAACGGCGCACGCCATCCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCGCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCtg                                                                                                                                                                                                      <  1:254309/251‑1 (MQ=255)                                                aTTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTgtgt                                                                                                                                                                                                   >  1:330894/1‑251 (MQ=255)                                                        aGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTCACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGAt                                                                                                                                                                                           >  2:327969/1‑251 (MQ=255)                                                              tCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTc                                                                                                                                                                                     >  1:679432/1‑251 (MQ=255)                                                               cgcgATCGCTGGTTTGTTGGGAGAACTGCGCCCGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTc                                                                                                                                                                                     <  1:407686/250‑1 (MQ=255)                                                                  gttcgcgggtgtggggtgagaACGGCGCCCGCCACCCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTGTTCAGGCTTTGGTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAAGGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGTTGTGTTCTGCGATGCGTTCCCAg                                                                                                                                                                                 <  2:615271/234‑1 (MQ=255)                                                                         ggTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGAc                                                                                                                                                                          >  1:36252/1‑251 (MQ=255)                                                                               ttgGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTACCCCGGTATTCAGGCTTTGTTGCGGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGa                                                                                                                                                                        >  2:63343/1‑247 (MQ=255)                                                                                tgGGAGAACTGCGCACGCCACCCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGAt                                                                                                                                                                    <  1:459833/250‑1 (MQ=255)                                                                                  ggAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTc                                                                                                                                                                 <  1:455580/251‑1 (MQ=255)                                                                                  ggAGAACGGCGCACTCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTc                                                                                                                                                                 <  2:376361/251‑1 (MQ=255)                                                                                   gagaACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCg                                                                                                                                                                >  2:187571/1‑251 (MQ=255)                                                                                     gaACTTCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGTAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTTCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAATCGCCATTGTGCTCTGTAAGCAATTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGTCGCGCTGTGTTCTTCTATGCGTTCCCAGTGTGGACGAAGATGTCGTg                                                                                                                                                              <  1:526045/251‑1 (MQ=255)                                                                                               cGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGc                                                                                                                                                    >  2:526462/1‑251 (MQ=255)                                                                                                gCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGcc                                                                                                                                                   >  1:170448/1‑251 (MQ=255)                                                                                                   aaCCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTCCTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGcc                                                                                                                                                   >  1:457947/1‑248 (MQ=255)                                                                                                      ccgccgGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTa                                                                                                                                             <  1:304630/251‑1 (MQ=255)                                                                                                                   gttCCACTGACGGAAGCGGTCGTGTTCCTGTTACCATGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACGTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGtt                                                                                                                                <  1:61581/251‑1 (MQ=255)                                                                                                                             ctgAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGTGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGaa                                                                                                                                                                       <  2:520278/200‑1 (MQ=255)                                                                                                                             cGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGaa                                                                                                                                                                       >  1:520278/1‑202 (MQ=255)                                                                                                                               gAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCtt                                                                                                                    <  2:443816/251‑1 (MQ=255)                                                                                                                                         tgtTCCTGTAAACAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCACCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTttcttcttc                                                                                                          >  2:528931/1‑251 (MQ=255)                                                                                                                                           ttCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCgg                                                                                                        >  2:611013/1‑251 (MQ=255)                                                                                                                                                 tAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGACGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACg                                                                                                  >  2:286776/1‑251 (MQ=255)                                                                                                                                                  aaCCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCGGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTGCTTCTTCGGCTAACg                                                                                                  <  1:474479/250‑1 (MQ=255)                                                                                                                                                    ccAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCt                                                                                               >  1:645605/1‑251 (MQ=255)                                                                                                                                                    ccAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCt                                                                                               >  2:594777/1‑251 (MQ=255)                                                                                                                                                       ggTATTCAGGCGTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTGTTCTTCTTCGGCTAACGCCTGtt                                                                                            <  2:81347/251‑1 (MQ=255)                                                                                                                                                         tATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGc                                                                                          >  1:616895/1‑251 (MQ=255)                                                                                                                                                             cAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGTGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACTAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGGTGAGGTTGCGGTTTTTCTTCTTCGGGGAACGCCTGTTGCAAg                                                                                       <  2:81041/250‑1 (MQ=255)                                                                                                                                                             cAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAgg                                                                                      >  1:14480/1‑251 (MQ=255)                                                                                                                                                                 cTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCct‑g                                                                                 >  1:513604/1‑251 (MQ=255)                                                                                                                                                                       tgctgctgcTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGAcg                                                                           >  1:171837/1‑251 (MQ=255)                                                                                                                                                                       tgctgctgcTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGAcg                                                                           >  2:292978/1‑251 (MQ=255)                                                                                                                                                                       tgctgctgcTGTAAGTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCTTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGAc                                                                            <  2:65014/250‑1 (MQ=255)                                                                                                                                                                       ggatgctgcgttAATTCGGCTGTCTGCTTCGCCGCGTGGTGGCGAATGCTCGCTCGAAGCGTCATTGTGCTCGGGAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATTCGCCATCGCCGGGGTGTGGTCTGCGATGTGTGTGCAGTGTGGACGAAGATTTCGTGCCGGTTGTTCCATACTAAGCGCCGCCAGTGGAGGTTTCGCGGGGTCTTCTTCGGCTAGCGCCTGTTGCAAGGCctacttac                                                                             <  2:686566/239‑7 (MQ=255)                                                                                                                                                                        gctgctgctGTCACTCTGCTGACTGCTTAGCCCCGTGGTAGAGAATGCTAGCGCGCAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTACTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGCTGCGTTCCCCGTGTCGACGAAGATTTCGTGCCGGTTGTGCCACACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTACCGACTGTTGCAAGGCCT‑GCTGAcgg                                                                          >  2:413896/1‑251 (MQ=255)                                                                                                                                                                         ctgctgctgTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGACGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGAcggc                                                                         >  2:149185/1‑251 (MQ=255)                                                                                                                                                                          tgctgctgTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCCGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTCCGCCTAACGCCTGTTGCA‑GGCCTTGCTGAcgccg                                                                        >  2:480183/1‑248 (MQ=255)                                                                                                                                                                              gctgTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCggctg                                                                    <  1:215838/251‑1 (MQ=255)                                                                                                                                                                              gctgTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCggct                                                                     <  1:211298/250‑1 (MQ=255)                                                                                                                                                                              gctgTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGGGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCggct                                                                     <  1:418249/250‑1 (MQ=255)                                                                                                                                                                              gctgTAAGTCTGCTGACTGCGTCGCCCCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATCTGCTCCAGCGCCGCGCTGTGTTCTGCGATGCGTCCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTGTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCggctg                                                                    <  2:259122/251‑1 (MQ=255)                                                                                                                                                                                   aaTTCTGCTGACTGCTTCGCCGCTTGGTGGCGACTGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTTCAAGGCCT‑GCTGACGGCGTCTGGcttc                                                               >  2:120161/1‑251 (MQ=255)                                                                                                                                                                                   aaTTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGctt                                                                <  2:328658/250‑1 (MQ=255)                                                                                                                                                                                   aaTTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGCATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGcttc                                                               >  1:624818/1‑251 (MQ=255)                                                                                                                                                                                         gctgACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAGGCACGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGAGATGCGTTCCCAGTGTGGACAAAGATTTAGTGCGGGTTGGGCCAGAc                                                                                                                                               >  2:285537/1‑166 (MQ=255)                                                                                                                                                                                         gctgACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGAc                                                                                                                                               <  1:285537/166‑1 (MQ=255)                                                                                                                                                                                         gctgACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTtgctg                                                         >  2:452897/1‑251 (MQ=255)                                                                                                                                                                                              cTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGACGCGCCATTGTGCTCTGTCAGCACGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGCTCTGCGCTGCGTTCCCAGTGTGGACGACGATTTCGTGCCGGCTGTGCCCGCCTAAGCGCCGCCAGTTGCGGTTGCGCTTTTTCTTCTTCGGCTCACGCCTGTTGCCAGCCCC‑TCTGACggcggcccgccccttcctccactc                                                    >  2:210937/1‑233 (MQ=255)                                                                                                                                                                                              cTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGACGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATATGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCAAGACTAAGCGCCGCCAGTTGAGGCTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGGTTCTTGCTGCAAtt                                                    >  2:38539/1‑251 (MQ=255)                                                                                                                                                                                                    cgccgcGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTcc                                               <  1:635907/250‑1 (MQ=255)                                                                                                                                                                                                      ccgcGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTg                                             >  1:228082/1‑250 (MQ=255)                                                                                                                                                                                                         cgTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGAcgc                                         >  2:294327/1‑251 (MQ=255)                                                                                                                                                                                                            ggtggCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGtt                                      <  1:327969/251‑1 (MQ=255)                                                                                                                                                                                                            ggtggCGAAGGCTCGCGCGAAGTGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGt                                       <  2:638989/250‑1 (MQ=255)                                                                                                                                                                                                                 cGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAAcc                                  >  2:249745/1‑250 (MQ=255)                                                                                                                                                                                                                   aaTGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAACCAGt                               >  1:486319/1‑251 (MQ=255)                                                                                                                                                                                                                     tGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCct‑g                                                                                 <  2:249833/199‑1 (MQ=255)                                                                                                                                                                                                                     tGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAACCAGttt                             >  1:468398/1‑251 (MQ=255)                                                                                                                                                                                                                     tGCTCGCGCGAAGCGCAATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCct‑g                                                                                 >  1:249833/1‑199 (MQ=255)                                                                                                                                                                                                                         cgcgcgAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAACCAGTTTAGCg                         >  1:173522/1‑251 (MQ=255)                                                                                                                                                                                                                             cgAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAACCCGTTTAGCGAttg                     >  1:615786/1‑251 (MQ=255)                                                                                                                                                                                                                              gAAGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAACCAGTTTAGCGAttgt                    >  1:210781/1‑251 (MQ=255)                                                                                                                                                                                                                               aaGCGCCATTGTTCTCTGTAAGCAAGTATTTACTTCGTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTTCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGTCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAACCAGTTTAGCGAttgtt                   <  2:273891/251‑1 (MQ=255)                                                                                                                                                                                                                                aGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAACCAGTTTAGCGAttgttg                  >  2:179585/1‑251 (MQ=255)                                                                                                                                                                                                                                aGCGCCATTGTGCTCTGTAAGCAAGTATTTACTTCTTCAATCGGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAACCAGTTTAGCGAttgttg                  <  1:491213/251‑1 (MQ=255)                                                                                                                                                                                                                                                gTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAACCAGTTTAGCGATTGTTGTTCTTGCTGCTGcgcg  >  1:398088/1‑251 (MQ=255)                                                                                                                                                                                                                                                gTAAGCAAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAACCAGTTTAGCGATTGTTGTTCTTGCTGCTGcgcg  >  1:617207/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      CGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTGACGGAAGCGGTCGTGTTCCTGTAACCAGGTATTCAGGCTTTGTTGCTGCTGCTGTAATTCTGCTGACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCT‑GCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAACCAGTTTAGCGATTGTTGTTCTTGCTGCTGCGCG  >  NC_000913/414554‑415043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑