Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,043,522	C→A	noncoding (36/90 nt)	serU ←	tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,043,522	0	C	A	97.1%	97.4 / ‑6.6	34	noncoding (36/90 nt)	serU	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (19/14);  minor base G (1/0);  total (20/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAACTGCACATTTTCGCTCGAAAGCTTCCTGTCAGATCCATAGCGAATCAAGTGCTGAATGTCACAGTATCGAACAGAAAACAGTGACGATCTAACCCTTCAAGAATATTCTACGATTGTTCTGTTTAGGAAAAGCAAGGCGGGAAGTCGGGAGATAAGTCATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCC‑TACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGCCCTTCCCTGGCAGGAAGCACTATCGATCCCCCTTTTAACGTGTCTGACGGAACAGGAACAAAGCAAATT  >  NC_000913/2043296‑2043766                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     aTAACTGCACATTTTCGCTCGAAAGCGTCCTGTCAGATCCATAGCGAATCAAGTGCTGAATGTCACAGTATCGAACAGAAAACAGTGACGATCTAACACTTCAAGAATATTCTACGATTGTTCTGTTTAGGAAAAGCAAGGCGGGAAGTCGGGAGATAAGTCATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACGCCCGGTAGAGATGACCC‑TACTCCAGTATTAGAGACCgggacgttagggcgttcc                                                                                                                                                                                                                              >  2:187645/1‑235 (MQ=255)   aaCTGCACATTTTCGCTCGAAAGCTTCCTGTCAGATCCATAGCGAATCAAGTGCTGAATGTCACAGTATCGAACAGAAAACAGTGACGATCTAACCCTTCAAGAATATTCTACGATTGTTCTGTTTAGGAAAAGCAAGGCGGGAAGTCGGGAGATAAGTCATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCC‑TACTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCg                                                                                                                                                                                                                             <  1:148032/250‑1 (MQ=255)         acaTTTTCGCTCGAAAGCTTCCTGTCAGATCCATAGCGAATCAAGTGCTGACTGTCACAGTATCGAACAGAAAACAGTGACGATCTAACCCTTCAAGAATATTCTACGATTGTTCTGTTTAGGCAAAGCAAGGCGGGAAGTCGGGAGATAAGTCATTGATACAGTGGCGGAGAGAGGGGGGTTTTAACCCCCGGTAGAGTTGCCCC‑TACCCCGGTATTAAAGACCGGTCCGTTAAGCCGCTCCGGCaactt                                                                                                                                                                                                                      >  1:417593/1‑247 (MQ=255)                  cTCGAAAGATTCCTGTCAGATCCATAGCGAATCAAGTGCTGAATGTCACAGTATCGAACAGAAAACAGTGACGATCTAACCCTTCAAAAATATTCTACGATTGTTCTGTTTAGGAAAAGCAAGGCGGGAAGTCGGGAGATAAGTCATTGATAAAGTGGCGGAGAGAGGGGGATTTGCACCCCCGGGAGAGGTGCCCC‑TACTCCGGTTTCAGAGACCGGGCCGTTCAGCCGCCCCGGCATCTCTCCGtttcg                                                                                                                                                                                                             >  1:600846/1‑248 (MQ=255)                     gAAAGCTTCCTGTCAGATCCATAGCGAATCAAGTGCTGAATGTCACAGTATCGAACAGAAAACAGTGACGATCTAACCCTTCAAGAATATTCTACGCTTGTTCTGTTTAGGAAAAGCAAGGCGGGAAGTCGGGAGATCAGTCATTGATAAAGTGGCGGAGAGAGGGGGGTTTGAAACCCCGGTAGAGATGCCCC‑CCCTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCAACTCTCCGTTcccatg                                                                                                                                                                                                          >  1:318198/1‑246 (MQ=255)                                     agccATAGCGAATCAAGTGCTGAATGGCACAGTTTCGAACAGAAAACAGTGACGATCTAACCCTTCAAGAATATTCTACGATTGTTCTGTTTAGGAAAAGCAAGTCGGGAAGTTGTGAGATAAGTCATTGATAAAGTGGCGTAGAGAGGGGGATTTGAACCTGCGGTAGAGTTGCCCC‑TACTTCGGTTTTAGAGACCTGTTCGTTTAGCCTCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAg                                                                                                                                                                                          <  1:341210/249‑1 (MQ=255)                                              gAATCAAGTGCTGAATGTCACAGTATCGAACAGAAAACAGCGACGATCTAACCCTTCAAGAATATTCTACGATTGTTCTGTTTAGGAAAAGCAAGGCGGAAAGTCGGGAGATAAGTCATTGATAAAGTGGCGGAGAGAGGGGGCGGTGCACCCCCCGAAGAGGTGCCCC‑GACGCCGGTTTTAGAGGCAGGGCCGTGCAGCCGCCCCCGGAACCCCCCGTTCAGATGGGTGGCatgcttgcaggcaaattgg                                                                                                                                                                                 >  2:67287/1‑235 (MQ=255)                                                       gCTGAATGTCACAGTATCGAACAGAAAACAGTGACGATCTAACCCTTCAAGAATATGCTACGATTGTTCTGGTTAGGAAGAGCAAGGCGGGAAGTCGGGAGATAAGTCATTGATAAAGTGGCTGAGAGAGGGTGATTTGAACCCCCGGTAGAGGTGCCCC‑TACGCCGGTTTTAGAGACCGGTCCGTGCAGCCGCTCCGTCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAAc                                                                                                                                                                        <  2:270857/251‑1 (MQ=255)                                                                   aGTATCGAACAGAAAACAGTGACGATCTAACCCTTCAAGAATATTCTACGATTGATCTGTTTTGGAAAAGCAAGGCGGGCAGTCGGGAGGTAAGTCATTGATAAAGTGGCGGAGAGAGGGGGATTTTAACCCCCGGTAAAGTTGCCCCGTATTCAGGTTT‑GAAGACCTGGCCGTTCTGCAGCTCCGGCATCACTACGTTCAGATTGATGCCATTATGCCCGGGAATTTGGCACTTTAACAAGCCCTGttcg                                                                                                                                                            >  2:395524/1‑248 (MQ=255)                                                                                     gTGACGATCTAACCATTCAAGAATATTCTACGATTGTTCTGTTTAGGAAAAGCAAGGCGGGAAGTCGGGAGATAAGTCATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGGTGCCCC‑TACTCCGGTTTTAGAGACCGGGCCGTTCAGCCGCTCCGGCATCTCTCCCTTCAGATGGTTGCCCTGATGCCAGGAAATTTGGCCTTTTAACAATCCCTGTCCGTGCCATTTTTTTCAAGTg                                                                                                                                          >  1:193485/1‑251 (MQ=255)                                                                                                aCCCTTCAAGAATATTCTACGATTGTTCTGTTTAGGAAAAGCAAGGCGGGAAGTCGGGAGATAAGTCATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCC‑TACTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCg                                                                                                                               <  2:180250/251‑1 (MQ=255)                                                                                                  ccTTCAAGAATATTCTACGATTGTTCTGTTTAGGAAAAGCAAGGCGGGAAGTCGGGAGATAAGTCATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGGAGAGGTGCCCC‑TACTCCGGATTTAGAGACCGGTCCGTTCAGCCGCTCCCGCATCTATCCCTTCAGATGGGTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGTCGAGttttcg                                                                                                                               >  2:473286/1‑246 (MQ=255)                                                                                                         gAATATTCTACGATTGTTCTGTTTAGGAAAAGCAAGGCGGGAAGTCGGGAGATAAGTCATTGATAAAGTGGCGGAGAGAGGGGGGTTTGAACCCCCGGGAGAGTTGCCCC‑TACTCCGGGTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCCGGAAATTTGGCCTTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACg                                                                                                                      >  1:306234/1‑251 (MQ=255)                                                                                                                  aCGATTGTTCTGTTTAGGAAAAGCAAGGCGGGAAGTCGGGAGATAAGTCATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCC‑TACTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTa                                                                                                               <  1:578673/249‑1 (MQ=255)                                                                                                                               ttAGGAAAAGCAAGGCGGGAAGTCGGGAGATAAGTCATTGCTACAGTGGCGGAGAAAGGGGGATTTGAAACCCCCGTAGAGTTGCCCA‑TACTCCGGTTTTAGAGACCGGTCCGGTCAGCCGCTCCGGCATATCTCCGTTCAGATGGGTGCCATGATGCCAGGAAATTTGGGATTTTAACAGTACCTGTCCGTGGAATTTTGTTCAAGTGGCGAGTTTGCGAGAAAAACAGTGATTAAGAGGCCCTggca                                                                                                  >  2:621336/1‑247 (MQ=255)                                                                                                                                 aGGAAAAGCAAGGCGGGAAGTCGGGAGATAAGGCATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCC‑TACTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTa                                                                                              <  2:207475/251‑1 (MQ=255)                                                                                                                                     aaaGCAAGGCGGGAAGTCGGGAGATAAGTCATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGGAGAGTTGCCCC‑TACTCCGGGTTTAGAGACCGGTCCGTTCCGCCGCTCCGGCATCTCTCCCTTCAGATGGTTGCCATGCTGCCAGGAACATTGGCCTTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGCGTTTGCGAGCAAAACCATGATTAAGTGGCCCTGGGAAATACaa                                                                                           >  2:496750/1‑250 (MQ=255)                                                                                                                                         cAAGGCGGGAAGTCGGGAGATAAGTCATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCC‑TACTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATc                                                                                      <  1:330058/251‑1 (MQ=255)                                                                                                                                               gggAAGTCGGGAGATAAGTCATTGATAAAGTGGCGGAGAGAGGGGGATTGGAACCCCCGGTAGAGTTGCCCC‑TACTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCAGGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGcaca                                                                                <  1:67287/251‑1 (MQ=255)                                                                                                                                                                gTCAATGATAAAGTGGCTGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCC‑TACTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGCCCTTCCCt                                                               <  2:195958/251‑1 (MQ=255)                                                                                                                                                                  cATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCC‑TACTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGCCCTTCCCTgg                                                             <  1:159415/251‑1 (MQ=255)                                                                                                                                                                      gATAAAGTGGCGGAGAGAGGGGGATTTGCACCCCCGGTAGAGTTGCCCC‑TACTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTGCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGCCCTTCCCTGGCAg                                                          <  2:159811/250‑1 (MQ=255)                                                                                                                                                                              ggCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCC‑TCCTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTGCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGCCCTTCCCTGGCAGGAAGCACt                                                  <  2:85944/250‑1 (MQ=255)                                                                                                                                                                              ggCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCC‑TACTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGAGACGAGTTTGCGAGCAAAACGCTGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGCCCTTCCCTGGCAGGAAGCACt                                                  >  1:437188/1‑250 (MQ=255)                                                                                                                                                                                 ggAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCC‑TACTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGCCCTTCCCTGGCAGGAAGCACTATCg                                              >  2:123237/1‑251 (MQ=255)                                                                                                                                                                                         ggggATTTGAACCCCCGGTAGAGTTGCCCC‑TACTCCGGTTTTAGCGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGCTGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGCATCAGCACATCAAACTGCCCTTCCCTGGCCGGAAGCACTATCGATCCCCCt                                      >  2:590370/1‑251 (MQ=255)                                                                                                                                                                                         ggggATTTGAACCCCCGGTAGAGTTGCCCC‑TACTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGCCCTTCCCTGGCAGGAAGCACTATCGATCCCCCt                                      >  1:437099/1‑251 (MQ=255)                                                                                                                                                                                               ttGAACCCCCGGTAGAGTTGCCCC‑TACTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGCCCTTCCCTGGCAGGAAGCACTATCGATCCCCCTTTTaa                                 <  1:608866/250‑1 (MQ=255)                                                                                                                                                                                                      cccGGAAGTGTTGACCG‑TACTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACACGAATCAGCACATCAAACTGCCATTCCCTGGCAGGAAGCACAACCGATCCCCCTTTTAACGTGTCTg                         >  2:674028/1‑251 (MQ=255)                                                                                                                                                                                                            agagTTGCCCC‑TACTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGCCCTTCCCTGGCAGGAAGCACTATCGATCCCCCTTTTAACGTGTCTGACgga                    >  1:634428/1‑250 (MQ=255)                                                                                                                                                                                                                gtGCCCC‑TACTCCGGTTTTAGAGACCGGGCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCAGTTTAACAGTCCCTGTCCGTGCAATTGTGTTCAAGGGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAAGCAGCACATCAAACTGACCTTCCCTGGCAGGAAGCACTATCGAGCCCCCTTTTAACGTGTCTGACggaacagg               <  2:63130/250‑1 (MQ=255)                                                                                                                                                                                                                     cc‑TACTCCGGTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGCCCTTCCCTGGCAGGAAGCACTATCGATCCCCCTTTTAACGTGTCTGACggaacaggaaca           >  2:221568/1‑250 (MQ=255)                                                                                                                                                                                                                          cTCCGGTTTTAGAGACCGGTCCGTTCAGCAGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGCCCTTCCCTGGCAGGAAGCACTATCGATCCCCCTTTTAACGTGTCTGACGGAACAGGAACAAAGc       >  2:349892/1‑250 (MQ=255)                                                                                                                                                                                                                               gTTTTAGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAACTTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTCCAAGAATCAGCACATCAAACTGCCCTTCCCTGAAAGGAAGCACTATCGATCCCCCTTTTAACGTGTCTGACGGAACAGGAACAAAGCAAAtt  >  2:602610/1‑250 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     ATAACTGCACATTTTCGCTCGAAAGCTTCCTGTCAGATCCATAGCGAATCAAGTGCTGAATGTCACAGTATCGAACAGAAAACAGTGACGATCTAACCCTTCAAGAATATTCTACGATTGTTCTGTTTAGGAAAAGCAAGGCGGGAAGTCGGGAGATAAGTCATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCC‑TACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGCCCTTCCCTGGCAGGAAGCACTATCGATCCCCCTTTTAACGTGTCTGACGGAACAGGAACAAAGCAAATT  >  NC_000913/2043296‑2043766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑