Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,817,734	C→T	C256Y (TGT→TAT)	chbF ←	monoacetylchitobiose‑6‑phosphate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,817,734	0	C	T	100.0%	95.2 / NA	28	C256Y (TGT→TAT)	chbF	monoacetylchitobiose‑6‑phosphate hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/15);  total (13/15)

ACGTAATGTTCTGCTTGCTTGTCGTTGTAGATAGCGTTGATCACTTCGCATGCTGCATCAGAGTAATAAGCCCCACCGCGCTGTTCCAGTTCTTTCGGCTTAACTTTCAGCTCAGGATTTTTATACAGCTCAAAAAGTTGTTTCTCGACTTTCTGTACTACCTGCGCTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCTTCATGCCGATC  >  NC_000913/1817488‑1817976                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    acgttgtgtgcTGCTTTCGTGTCGTTGTAGATAGCGTTGATCACTTCGCATGCTGCATCAGTGTAATAAGCCCCACCGCGCTGTTCCAGGTCTTTCGGCTTAACTTTCAGCTCTGGATGTTTATACAGCTCAAATATTTGTGTCGCGACGTTCTGTACGACCTGGGCTCGGGTGGCGCCTTGGTGGTATTCGCCCATTTCGATAATCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAATATgg                                                                                                                                                                                                                                                 <  1:328251/241‑1 (MQ=255)  cGTAATGTTCTGCGTGCTTGTCGTTGTAGATAGCGTTGATCACTTCGCATGCTGCATCAGAGTAATAAGCCCCACCTCGCTGTTCCAGTTCTTTCGGCTTAACTTTCAGCTCAGGATGTTTATACAGCTCAAAAAGTTGTTTCTCGACTTTCTGTACTACCCGCGCTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGAGAAGAATATGgc                                                                                                                                                                                                                                                <  2:652746/251‑1 (MQ=255)     aaTGTTCTGCTTGCTTGTCGTTGTAGATAGCGTTGATCACTTCGCATGCTGCATCAGAGTAATAAGCCCCACCGCGCTGTTCCAGTTCTTTCGGCTTAACTTTCAGCTCAGGATTTTTATACAGCTCAAAAAGTTGTTTCTCGACTTTCTGTACTACCTGCGCTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAATATGgc‑ag                                                                                                                                                                                                                                             <  1:201078/250‑1 (MQ=255)        gTTCTGCTTTCTTGTCGTTGTAGATAGCGTTGATCACTTCGCATGCTGCATCAGAGTAATAAGCCCCACCGCGCTGTTCCAGTTCTTTCTGCTTAACTTTCAGCTCAGGATTTTTATACAGCTCAAAAAGTTGTTTCTCGACTTTCTGTACTACCTGCGCTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTTCTTGAAGTAATACAGCATATAAGAATAGGGc‑agcag                                                                                                                                                                                                                                          <  2:497159/250‑1 (MQ=255)         ttCTGCTTGCTTGTCGTTGTAGATAGCGTTGATCACTTCGCATGCTGCATCAGAGTAATAAGCCCCACCGCGCTGTTCCAGTTCTTTCGGCTTAACTTTCAGATCAGGATTTTTATACCGCTCAAAAAGTTGTTTCTCGACTTTCTGTACTACCTGCGCTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAATATGgc‑agc                                                                                                                                                                                                                                            >  1:482937/1‑247 (MQ=255)                  cttgTCGTTGTAGATAGCGTTGATCACTTCGCATGCTGCATCAGAGTAATAAGCCCCACCGCGCTGTTCCAGTTCTTTCGGCTTAACTTTCAGCTCAGGATTTTTATACAGCTCAAAAAGTTGTTTCTCGACTTTCTGTACTACCTGCGCTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAATATGGC‑AGCAGATTCaacgaac                                                                                                                                                                                                                               >  2:596123/1‑251 (MQ=255)                               aTAGCGTTGATCACTTCGCATGCTGCATCAGAGTAATAAGCCCCACCGCGCTGTTCCAGTTCTTTCGGCTTAACTTTCAGCTCAGGATTTTTATACAGCTCAAAAAGTTGTTTCTCGACTTTCTGTACTACCTGCGCTCGTGCACCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTc                                                                                                                                                                                                                  >  1:85244/1‑251 (MQ=255)                                              gcGCATGCTGCATCAGAGTAATAAGCCCCACCGCGCTGTTCCAGTTCTTTCGGCTTAACTTTCAGCTCAGGATTTTTATACAGCTCAAAAAGTTGTTTCTCGACTTTCTGTACTACCTGCGCTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGAt                                                                                                                                                                                                    <  1:678917/249‑1 (MQ=255)                                                                    aaGCCCCACCGCGCTGTTCCAGTTCTTTCGGCTTAACTTTCAGCTCAGGATTTTTATACAGCTCAAAAAGTTGTTTCTCGACTTTCTGTACTACCTGCGCTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGa                                                                                                                                                                              <  1:512185/250‑1 (MQ=255)                                                                                      ccatttCTTTCGGTTTAACTTCCAGCTCAGGATTTTTATACAGCTCAAAAATTTTTTCCTCGACTTTCGGTCCTACCTCCGCCCGTGCGCCGCTTTTTTATTATTCGCCCATTTAAATAGCCGGCATCTCTTTTTGTTTAAATAAATCCAG‑AAATAAAAATAAGGCCAGCA‑AATCCACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACg                                                                                                                                                          <  1:697702/247‑1 (MQ=255)                                                                                                cGGCTTAACTTTCAGCTCAGGATTTTTATACAGCTCAAAAAGTTGTTTCTCAACTTTCTGTACTACCTGCGCTCGTGCGCAGCCTTTGTAGTATTCGACCATTTCAAAAGCCAGCATCTATTTCTGCTTGACGTAATACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCATCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCAt                                                                                                                                                 >  1:357191/1‑251 (MQ=255)                                                                                                                                         ctgtaacaaatattttCTGTACTACCTGCGCTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGCTCGAAAATATTTTTTACag                                                                                                                                                                                  >  2:642670/14‑177 (MQ=255)                                                                                                                                         ctgtaaaaaatattttCTGTACTACCTGCGCTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACag                                                                                                                                                                                  <  1:642670/164‑1 (MQ=255)                                                                                                                                              ttCTCGGCTTTCTGTACTACCTGCGCTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCtt                                                                                                   >  2:139048/1‑251 (MQ=255)                                                                                                                                                                               ccgtctgtgtcgtaatcgaccttttCAATAGCCAGCATCTCTTTCCGCTTGAAGTAATACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATTGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAATCAATGCGGCTAAGCGCGACTTGCCATTTATCAGCCCATCCGTAATGAACACCATATGGTTGATGCTGAACATATc                                                                  <  2:482937/229‑1 (MQ=255)                                                                                                                                                                               ccgccTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAATATGGA‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGAGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATc                                                                  >  1:477208/1‑251 (MQ=255)                                                                                                                                                                                cgccTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCg                                                                 <  1:524110/251‑1 (MQ=255)                                                                                                                                                                                              tCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATAGTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAatcat                                                    <  1:416225/250‑1 (MQ=255)                                                                                                                                                                                               cGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAatcatc                                                   <  1:242354/250‑1 (MQ=255)                                                                                                                                                                                                         aaTAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTc                                         >  1:704526/1‑250 (MQ=255)                                                                                                                                                                                                             gCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTAGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGTGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCAt                                    >  1:20181/1‑251 (MQ=255)                                                                                                                                                                                                                       ctTTCTGCTTGAAGTAATACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACa                             <  1:51786/248‑1 (MQ=255)                                                                                                                                                                                                                                    gTAATACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCt             <  2:704526/251‑1 (MQ=255)                                                                                                                                                                                                                                     tAATACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt            >  1:227281/1‑251 (MQ=255)                                                                                                                                                                                                                                      aaTACAGCAGATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt            <  1:503799/250‑1 (MQ=255)                                                                                                                                                                                                                                          cagcagATAAAAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGAGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCTTCATGc       >  2:286237/1‑251 (MQ=255)                                                                                                                                                                                                                                               gATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCTTCATGCCGATc  >  1:486757/1‑251 (MQ=255)                                                                                                                                                                                                                                               gATAAGAATATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCTTCATGCCGATc  >  1:263626/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    ACGTAATGTTCTGCTTGCTTGTCGTTGTAGATAGCGTTGATCACTTCGCATGCTGCATCAGAGTAATAAGCCCCACCGCGCTGTTCCAGTTCTTTCGGCTTAACTTTCAGCTCAGGATTTTTATACAGCTCAAAAAGTTGTTTCTCGACTTTCTGTACTACCTGCGCTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGC‑AGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCTTCATGCCGATC  >  NC_000913/1817488‑1817976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑