Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,753,680	G→A	P51S (CCT→TCT)	ydhV ←	putative oxidoreductase YdhV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,753,680	0	G	A	100.0%	105.1 / NA	34	P51S (CCT→TCT)	ydhV	putative oxidoreductase YdhV
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/17);  total (17/17)

CTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCAT  >  NC_000913/1753452‑1753923                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    cTTTAATCTTCAGACATACCGGTGATTTCGCCTTCACTTCGCTATTAATGACGTCGTATCCAGCGAATTTCTTTTGCGCTGCAACCAAGCCACCCCTATGGGCATCGACGAATAAATTTCCTTTGGTAACAGTAGAAAGTGAGGTGATATTTCAGCGAGAACTACTGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGGACTTCg                                                                                                                                                                                                                               >  2:688572/1‑251 (MQ=255)          tCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTcataca                                                                                                                                                                                                                        <  1:110110/249‑1 (MQ=255)                             cGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGccc                                                                                                                                                                                                    >  1:639600/1‑250 (MQ=255)                                 ttcccttcGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGcc                                                                                                                                                                                               >  2:570494/1‑251 (MQ=255)                                      ttcgatcaaaatgccgacgaaTCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTAAAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCg                                                                                                                                                                                           >  1:540708/21‑250 (MQ=255)                                                      tcgtATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACtt                                                                                                                                                                          >  2:204424/1‑251 (MQ=255)                                                            ccAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTgg                                                                                                                                                                                                                                         <  1:112794/182‑1 (MQ=255)                                                            ccAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTgg                                                                                                                                                                                                                                         >  2:112794/1‑182 (MQ=255)                                                                           tGCGCTGCAAAAAAGCCACCCATAGGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATTGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACAGAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGTATTCTTCGAGGGTaa                                                                                                                                                      <  2:642711/250‑1 (MQ=255)                                                                              gcTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATAtt                                                                                                                                                  >  1:36413/1‑251 (MQ=255)                                                                                  cAAAAACGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGGAATATTTCCt                                                                                                                                              >  1:273255/1‑251 (MQ=255)                                                                                     aaaaGCCACCCATATGGGCATCGACGGCTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTc                                                                                                                                           <  2:16702/251‑1 (MQ=255)                                                                                        aGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTGATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGTTAATATTTCCTGTCGTg                                                                                                                                        <  1:519076/251‑1 (MQ=255)                                                                                           cACCCATATGGGCATCGACGACTAAATTTCCTTTGGTACAAGTAGAAAGTTAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTg                                                                                                                                        >  2:654847/1‑248 (MQ=255)                                                                                              ccATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATGTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAGACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTg                                                                                                                                  <  1:40844/251‑1 (MQ=255)                                                                                                        aTCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCAGCGAAAGATTGCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCAGGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAata                                                                                                                        <  1:668084/251‑1 (MQ=255)                                                                                                            acgacTACATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGCGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATAtt                                                                                                                    >  2:307046/1‑251 (MQ=255)                                                                                                             cgacTCAATTTCCTTTGGTAACAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCg                                                                                                                                          >  2:94591/1‑228 (MQ=255)                                                                                                             cgacTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCg                                                                                                                                          <  1:94591/228‑1 (MQ=255)                                                                                                                             cgTAAAAGTTGAAAGTGAAGTGATAGTTACGCGAGAACTACAGGTGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGACATACATAATTTTGTAGCCGAAGCCCATGCCACCGCCAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTCAGATTGACTCTTAATATATTACCTGTCCAACCGTTAg                                                                                                   <  2:198790/250‑1 (MQ=255)                                                                                                                                                   atatTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAg                                                                             <  2:189364/251‑1 (MQ=255)                                                                                                                                                         aCGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTcac                                                                       >  1:644656/1‑251 (MQ=255)                                                                                                                                                         aCGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTca                                                                        <  1:265634/250‑1 (MQ=255)                                                                                                                                                         aCGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTGCGAGGGTAATATGTCCTGTCGTGAGATTGACTCTTAATATATGTCCTGTCCAGCCGTTAGCCATGATTTTTTCCTTTGCAAGATTca                                                                        <  1:280705/250‑1 (MQ=255)                                                                                                                                                                                aGATCCAGGTAATGGGACGGTAGCAAAGACTAATTTATTCGCTTCAGCGAAAGATTTCGTGCCGGGCGGTACGTCGTCAGACATAATATTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGATGGTAATATTTCCTGTCGTGAGATTGACTCGTAATAGATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACTCATTAATATCTTTCGACTCGAt                                                <  2:292600/251‑1 (MQ=255)                                                                                                                                                                                          aaTGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCAACGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTaa                                       <  1:512006/250‑1 (MQ=255)                                                                                                                                                                                             gggCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTACTATCTTTCCACTCGATAATTTTTAACGcc                                   >  2:252540/1‑251 (MQ=255)                                                                                                                                                                                                                   tATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCTACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTgcgc             >  1:557980/1‑251 (MQ=255)                                                                                                                                                                                                                   tATTCGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTgcgc             >  2:154574/1‑251 (MQ=255)                                                                                                                                                                                                                       cGCTTCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCGTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCTTTTGCGCATTc         <  1:196609/251‑1 (MQ=255)                                                                                                                                                                                                                           tCATCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCAcc      <  1:307046/250‑1 (MQ=255)                                                                                                                                                                                                                           tCATCGAAAGATTTCGTGCCGGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACGGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATACACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCAcc      <  1:688572/250‑1 (MQ=255)                                                                                                                                                                                                                             aTCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGcc   >  1:173215/1‑250 (MQ=255)                                                                                                                                                                                                                              tCGAAAGATTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGCAGCCCATGCCACCGACCAAACTTTTAAACTTCCTGGAATCTTCGAGGCTAATATTTCCTGTCGTGGGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGCTTTTTTCCTTTGCAAGCTTCACACTGTACTATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCAt  >  1:697927/1‑251 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    CTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCAT  >  NC_000913/1753452‑1753923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑