Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	155.0 / NA	42	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/25);  total (17/25)

ATGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGC  >  NC_000913/1652087‑1652570                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                aTGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCagag                                                                                                                                                                                                                                           <  1:189530/251‑1 (MQ=255)    gtgtgtTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGt                                                                                                                                                                                                                                        <  2:500896/251‑1 (MQ=255)        gtTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATgg                                                                                                                                                                                                                                    <  2:570083/251‑1 (MQ=255)              gTTTATACTGGAGTTCGCCCTGGTGAGCAGTGTGCACTTGGATGGGAGGACATAGAGCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcag                                                                                                                                                                                                                                 <  2:646288/248‑1 (MQ=255)                        gAGTTCGCCCTGGTGAGCTGTGTGCCCTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGCGAAATTTAGCAAAAGACCGTTTCACGGTACCACAAACACAGGCGGGAACCACTCCGGGCATTCAACTTCTTAAGCCCGCCATCGACGCTCTCCGGGGTGAGATTAACTCAACGAGGCTGCGCAAAGAGCATCTCATTGTTGTTCACCCCAAAGAGTATGGcagacacgaaaaacc                                                                                                                                                                                                                     >  1:644229/1‑239 (MQ=255)                            tCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATATGAAAAATGGAACAATGAGTATCAGGAGAAATTTAGCAAAAGACCGGTTCACGATACCAAAAACACAGGCGGGAACCAATCGGGACATTGATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACAGTAACGAGACTGAGCAAAGAGCATATCATTGATGTGCGCCTCAGAGAGTATGGCATAACAGAAAAGCaaaaa                                                                                                                                                                                                                 <  2:270190/250‑1 (MQ=255)                                ccTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGACAAATGGAACAATGATGATCAGGAGAAATTTAGCAGCAGCCCGTTTCACGGTACCAAAAACACAGGCGGGGACCAATCGGGGCATTACTCTTTTTAAGCCAGCACTCGTCGCTCTCCGGAGCTAGGTGACGTTAACGAGAATGGGGAAAAAGACTATTATTGATGTTCACCTCCGAGGGGACGGGAGAACAAGAAAACAACAATGCAc                                                                                                                                                                                                            >  1:264843/1‑251 (MQ=255)                                    gTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCttt                                                                                                                                                                                                        <  1:185399/251‑1 (MQ=255)                                        gCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGttt                                                                                                                                                                                                    <  1:213809/251‑1 (MQ=255)                                          tgtgtgCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGAATGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAAAAAAACAAAAATGAACCTtttttttt                                                                                                                                                                                                  >  2:288305/1‑251 (MQ=255)                                           gtgtgCACGTGGATGGGAGGACAGAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCTCGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGtttt                                                                                                                                                                                                   <  2:30399/249‑1 (MQ=255)                                                         gggAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCCATCGCCGCCCTCCGGAGTCAGATGACATTAACGCGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACCAAAATGCACCTTTGTTTTTCAACCTGAAGTGTc                                                                                                                                                                                    >  1:331276/1‑250 (MQ=255)                                                          ggaggaCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaac                                                                                                                                                                                                                              <  2:517753/207‑1 (MQ=255)                                                          ggaggaCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAACCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaac                                                                                                                                                                                                                              >  1:517753/1‑207 (MQ=255)                                                           gaggaCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGAGTCATTCATCTTTCTAAAACAGCACTCGTCGCCCTCCGGAGTCAGATGACCTTAACGAGAATGAGCAAAGAGCACATCATTTCTTTTCACCTCCGAGAGTCTTGCAGAAAAGAAAAAAAAAAATACACCTTTTTTTTTGAACCTAGAGTGTCAGc                                                                                                                                                                                 >  2:130109/1‑251 (MQ=255)                                                                    gATCTGAAAAATGGAACAATGATGATCAGAAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCAACTTATTAAGCCAGCAATCCAAGCTCTTCGGAGGCAAATGACCTTAACGAAACTTAGCAAAGAGCATTTCCTTGATTGTCCCCTCATAGAATAAGTCATAGCAGAAAAACAACAATGCACCTTTTTTTTTCAACCTTAAGTGTCATCgaaaggaa                                                                                                                                                                         >  1:566062/1‑244 (MQ=255)                                                                         gAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTGTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAACGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAAt                                                                                                                                                                    <  2:264843/250‑1 (MQ=255)                                                                           aaaaTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATTGCAGATCAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAt                                                                                                                                                                 <  2:612021/251‑1 (MQ=255)                                                                              aTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGTAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGt                                                                                                                                                              <  2:550922/251‑1 (MQ=255)                                                                                ggAACAATGATGATCAGTAGAAATTTAGCAAAAGACCGTTTCACCGTACCAAAAACACAGGCGGTAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGa                                                                                                                                                            <  1:121420/251‑1 (MQ=255)                                                                                 gAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCCAACGGGGCATTCATATTATTAAACCAGCAATCCACGCTCTCCGGAGTCAGATGACATTAAAGAGAATGAGCAAAAAGCATATAATTGATGTTCACCTCAGAGAGAATGGCCGAACAAAAAAACAAAAATGCACCTTTGTTTTTCAAACTGAAGTGGCAGAGAGGGAAAAAAATTAAGGTGAc                                                                                                                                                           >  2:118601/1‑251 (MQ=255)                                                                                 gAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAc                                                                                                                                                           <  1:130109/251‑1 (MQ=255)                                                                                     aaTGATGATCAGGAGAGATTTAGCAAAAGACCGTTTCACGGTACCAAAAAAACAGGCGAGAAACAAACGGGGCATTCCTCTTATTAAGACAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAACTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTAAGCGAGAGTAAAAAATTATGGTGACCAtt                                                                                                                                                       >  2:123352/1‑251 (MQ=255)                                                                                       tgatgatcacgtgcactttAGCAAAAGACCGTTTCACGGTACCAAAAAAACAGGCGGAAACCAATCGGGGCATTCATATTCTTAACCCCGCAATCAACGCCCTCCCGCGTCAGCTGACATTAAAGCGACTGAGCAAAGAGCATATCATTGATGTTTACCTCAGAGAGCATGGCCGAACAGAAAAACAAAAATTCACCTTTGTTTTTAAAACTGGATGGTCAACGCGGTTAAAAAATTATAGTGACCAtttt                                                                                                                                                     >  2:41244/17‑251 (MQ=255)                                                                                         atgatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAAAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTAc                                                                                                                                                   >  1:199062/1‑251 (MQ=255)                                                                                           gatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCg                                                                                                                                                 <  1:116502/251‑1 (MQ=255)                                                                                             tCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGCCGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAAAAAAACAAAAATGCACCTTTGTTTTTTAACCTTAAGTGTCAGCGAGAGGAAAAAATTATGGTGACCATTTTACCGtt                                                                                                                                               >  1:135222/1‑251 (MQ=255)                                                                                                 gagaAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCTTTTTACCGTTGACt                                                                                                                                           <  1:218719/251‑1 (MQ=255)                                                                                                                                        acaGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCg                                                                                                    <  1:288305/251‑1 (MQ=255)                                                                                                                                              gggAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTcc                                                                                              <  1:248131/251‑1 (MQ=255)                                                                                                                                                    cAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTc                                                                                                                                                                                    >  2:3542/1‑159 (MQ=255)                                                                                                                                                          ggTCATTCATCTTAGTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAAGTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAAt                                                                                  <  2:86327/251‑1 (MQ=255)                                                                                                                                                             cattcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGCGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCat                                                                               <  2:189007/251‑1 (MQ=255)                                                                                                                                                              attcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGGGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCat                                                                               >  1:272374/1‑250 (MQ=255)                                                                                                                                                                      tattaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTGGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTc                                                                        <  1:83922/249‑1 (MQ=255)                                                                                                                                                                          aaGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGAc                                                                   >  2:149698/1‑250 (MQ=255)                                                                                                                                                                            gCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATa                                                                <  1:149698/251‑1 (MQ=255)                                                                                                                                                                                                ggAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTGCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACGCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTg                                            <  2:676805/251‑1 (MQ=255)                                                                                                                                                                                                             aTTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCGGGTGCTa                               <  1:154059/251‑1 (MQ=255)                                                                                                                                                                                                                             aaaGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAAACTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATAAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTTCTAACCCGGCATTTATAGc               >  2:247390/1‑251 (MQ=255)                                                                                                                                                                                                                                    atatCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAAcc          >  1:104270/1‑249 (MQ=255)                                                                                                                                                                                                                                           tGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGc  <  2:156131/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                ATGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGC  >  NC_000913/1652087‑1652570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑