Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,494,227	A→T	I282K (ATA→AAA)	ydcI ←	DNA‑binding transcriptional dual regulator YdcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,494,227	0	A	T	100.0%	235.5 / NA	69	I282K (ATA→AAA)	ydcI	DNA‑binding transcriptional dual regulator YdcI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (27/42);  total (27/42)

CGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACA  >  NC_000913/1493983‑1494463                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             gggcggTGTCCCTTGAATAACTGGCGGAACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTTCGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTcaaa                                                                                                                                                                                                                                        <  1:584103/250‑4 (MQ=255)  gtcggTGTCCCTTGAAGAACAAGCGGCACGATTAACTGAGGCGTTGTATGGAGTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTTGCGGAGCGGGTGAACCAGGTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCAGCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAAt                                                                                                                                                                                                                                       <  2:453416/249‑1 (MQ=255)   gcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATc                                                                                                                                                                                                                                      >  1:73977/1‑251 (MQ=255)    cggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGAGGATGGATTACGTCTGCACAAACATTCTGGGTCGGCAGAAACTCGCCGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGTAAATGTTCAAGGAGGGATCGAAAGATCCCTTCACCTTTCAGAACGGCGTTGATTTTCGAATAGCGTTAATCATCCACTGGCCACCAGAAGAGAACGTCGCATCTACGCGGGGCAGTTTTCCAAt                                                                                                                                                                                                                                       >  1:214030/1‑249 (MQ=255)             ttGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGGTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGGTTTTCGAATAGCGTTAATCATCAACTGGGAACCCGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCCATCGGTTCGCCTg                                                                                                                                                                                                                            >  2:257637/1‑251 (MQ=255)                aagaaCAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGACCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCAc                                                                                                                                                                                                                         <  1:548952/251‑1 (MQ=255)                  gaaCAGGCGGCACGCTTAACTGAGGCGGTGGCTGTATTCCGTCTGCACAAACATTCTGTGTAGGCAGAACCGCGGGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGGTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAAAGGAATTGATTTTCGAATAGCGTTAATCATCCACTGGCAAACAGAAGAGCACGTCGCATCTACGCGGGTCAGTTTTCCCATCGGGTCGTCTGccccc                                                                                                                                                                                                                       >  2:463787/1‑247 (MQ=255)                     gaGGCGGAACGATTAACTGAGGCGGGGGATGGATTCCGTTTGCACAACCATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAAAGTTCAAGGAGGGATCGACAGATCCCTTCACCCTTCAGAACGGCCTTGATTTTCGAATAGCGTTAATCATCAACTGGCCACCAGAAGAGAAAGTTGCATCTACGCGGGACCGCTTTCCAATCGGTTCGCCCACACCATGt                                                                                                                                                                                                                    >  2:254750/2‑251 (MQ=255)                          ggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAAAATTCTGTGTCGGCCGAACCTCGCGGCGCGGGTGAACCAGTTAGTTTCGCTACGGGGTGACAATGTTCAAGGAGGGATCGACAGATACCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGCAGAGAACGTCGCCTCTACGCGGGTCAGTTTTCCCATCGGTTCGCCTGCCCCATGCCCCgg                                                                                                                                                                                                               >  2:290903/1‑251 (MQ=255)                               gATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAAcag                                                                                                                                                                                                          <  2:193453/251‑1 (MQ=255)                                       gAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGcc                                                                                                                                                                                                  >  1:227530/1‑251 (MQ=255)                                            ggTGGATGTATTCCGTCGGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCAGCAACTGGCAACCAGAAGAGAACGTCGCATCGACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAg                                                                                                                                                                                             <  2:567804/251‑1 (MQ=255)                                             gTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGc                                                                                                                                                                                            >  1:168404/1‑251 (MQ=255)                                                   gTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCTGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCa                                                                                                                                                                                      <  1:267504/251‑1 (MQ=255)                                                        ccGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGTGGAGCGGGGGAACCAGGTAGGTTCGCTACGGTGGGAAAATGTTCAAGGAGGGAGCGACAGATCCCTTCACCTTTCAGAACGGCAATGAGTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATTTACGCGGGTCAGTTTTCCAATCGGTCCGACTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGAc                                                                                                                                                                                  <  1:583201/250‑1 (MQ=255)                                                         cGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGc                                                                                                                                                                                >  2:448112/1‑251 (MQ=255)                                                              gCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgtc                                                                                                                                                                           >  1:521331/1‑251 (MQ=255)                                                              gCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgt                                                                                                                                                                            <  1:261239/250‑1 (MQ=255)                                                              gCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgt                                                                                                                                                                            <  1:463787/250‑1 (MQ=255)                                                              gCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTGCTAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCAGCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTTCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgt                                                                                                                                                                            <  1:40436/250‑1 (MQ=255)                                                                 caAACAGTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgtcgtc                                                                                                                                                                        <  1:602545/251‑1 (MQ=255)                                                                    aCATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCACTCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGGCGTCttt                                                                                                                                                                     >  1:237325/1‑251 (MQ=255)                                                                    aCATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCttt                                                                                                                                                                     <  1:465424/251‑1 (MQ=255)                                                                    aCATTCTGTGTCGGCAGAACCTCGCGGAACGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCAGCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCttt                                                                                                                                                                     <  2:261981/251‑1 (MQ=255)                                                                     cATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCtttt                                                                                                                                                                    <  2:356034/251‑1 (MQ=255)                                                                      aTTCTGTGGCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGACGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTa                                                                                                                                                                   <  2:273590/251‑1 (MQ=255)                                                                           gtgtCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAgc                                                                                                                                                               <  1:255705/250‑1 (MQ=255)                                                                                  cAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAAGCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAg                                                                                                                                                       <  1:434990/251‑1 (MQ=255)                                                                                          cgcgGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAAc                                                                                                                                               <  1:20567/251‑1 (MQ=255)                                                                                          cgcgGAGCGGGTGAACCAGTTAGTTTCGCTACGGGGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACaa                                                                                                                                                 <  2:168404/249‑1 (MQ=255)                                                                                                      gAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATc                                                                                                                                    <  2:14729/250‑1 (MQ=255)                                                                                                                gttTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCg                                                                                                                         <  1:476513/251‑1 (MQ=255)                                                                                                                 tttCGCTACGGTGTGAAAATGTTCTAGGCGCGATCGACAGATACCTTCACCTTTAAGAACGGCATTGATTTTCGAATAGCGTTACTCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACAAGCGTGGCATGCCGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGt                                                                                                                        >  2:86302/1‑251 (MQ=255)                                                                                                                    cGCTCCGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTCCAGCGCCCGAAGGGACAAACCACACGTAATCGTATTCAACCGTaa                                                                                                                      >  2:505888/1‑250 (MQ=255)                                                                                                                    cGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAg                                                                                                                     <  1:286274/251‑1 (MQ=255)                                                                                                                    cGCTACGGTGTGAAAATGGTCAAGGAGGGATCGACAGATCCATTCACCGTTCAGAACGGCATTGATTTGCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGGTTTCCAATCGGTTCGCCTGCACCATGTGCAGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCGTTTACAGCGCCAGAAGGGACAAACCACACGGAATCGTATTCAACCGTaa                                                                                                                      <  1:290737/250‑1 (MQ=255)                                                                                                                        aCGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGGCGCATCTACGCGGGTCAGTTTGCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGTCATGACGCAGGTCGTCTTTTACAGCGTCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGtt                                                                                                                   <  2:629734/249‑1 (MQ=255)                                                                                                                                     gTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGaa                                                                                                     <  1:257637/250‑1 (MQ=255)                                                                                                                                      ttCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCCTTGATTTTAGAATAGCGTTAATCCTCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCCTGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGc                                                                                                   >  2:470152/1‑251 (MQ=255)                                                                                                                                            gaggGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGAc                                                                                               >  1:381163/1‑249 (MQ=255)                                                                                                                                                     ccAGAACCCTACACTTTCCAGACCGGCATGGATTTCCGAATACGGTAAATCACAAACGGGAAACCAAAATAAAACGCCGCTTCTACGCGGGTAATTTTTCCAACCGGTCCGC‑TGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATa                                                                                   <  2:545903/250‑1 (MQ=255)                                                                                                                                                     aCAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTGCGCGGGTCAGGTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATATCGAAGCAGACAGCGTTTCGAt                                                                                    <  1:254750/251‑1 (MQ=255)                                                                                                                                                     aCAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGAt                                                                                    <  1:148718/251‑1 (MQ=255)                                                                                                                                                      cAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAATTTGACGAGATAGCGAAGCAGACAGCGTTTCGATa                                                                                   <  1:231610/251‑1 (MQ=255)                                                                                                                                                       aGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGTGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACTCGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATAc                                                                                  <  2:703236/251‑1 (MQ=255)                                                                                                                                                           cccTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAAc                                                                               <  1:136190/250‑1 (MQ=255)                                                                                                                                                               tctccTTACAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGa                                                                           <  2:201682/247‑1 (MQ=255)                                                                                                                                                                cACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCACCTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGa                                                                           >  1:56486/1‑249 (MQ=255)                                                                                                                                                                cACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAg                                                                         <  1:290903/251‑1 (MQ=255)                                                                                                                                                                 aCCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAgg                                                                        >  1:44380/1‑251 (MQ=255)                                                                                                                                                                   atttCAGAACGGCATTGCTTTTCGGATAGCGTCCATCATCAACTGGAAACCAGCAGCGAACGTCGAATATACGCGGATCAGATTTCCAATCGATTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGAATGACGCAGGGCGTCTTTTAAAGCGCCAGAAGGGACAAACCACACGTAACCGTATTCAAACGCAAGTTGACGAGATCGCGAAGCAGACAGCGGTTCGATACACCCCGACGGac                                                                      >  2:682389/2‑250 (MQ=255)                                                                                                                                                                      tCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGGCGCATCTACGCGGGTCAGGTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTGTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAAtt                                                                    <  2:294304/250‑1 (MQ=255)                                                                                                                                                                                          gAATAGCGTTAATCATCAACTGGCAACCCGAAGAGCACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGAACAAACCACACGTAATCGTATTCAACCGAAAGTTGACGAGATAGAGCAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGACCTGGCTCTgaacc                                               >  2:423917/1‑247 (MQ=255)                                                                                                                                                                                           aaTAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTa                                              <  2:44380/251‑1 (MQ=255)                                                                                                                                                                                             tAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGCCGCAGGTCGTCTTTTACAGCGCCAGACGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAAt                                            >  1:363444/1‑251 (MQ=255)                                                                                                                                                                                               gCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGCCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTAAAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGc                                          >  1:650428/1‑251 (MQ=255)                                                                                                                                                                                                   tAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATc                                       <  2:181768/250‑1 (MQ=255)                                                                                                                                                                                                     atcatcAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTATGCACTAATGCATCTg                                     >  1:536653/1‑250 (MQ=255)                                                                                                                                                                                                      tcatcaACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGa                                    >  2:535855/1‑250 (MQ=255)                                                                                                                                                                                                       catcaACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGaa                                   <  1:470152/250‑1 (MQ=255)                                                                                                                                                                                                           aaCTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCGGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCtg                              <  2:213135/251‑1 (MQ=255)                                                                                                                                                                                                           aaCTGGAAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTAAAGCCCGGGCTCAGCACTAATGCATCTGAATGCtg                              >  2:75163/1‑251 (MQ=255)                                                                                                                                                                                                               ggCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGGTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACGCGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCtggc                            <  1:646890/249‑1 (MQ=255)                                                                                                                                                                                                                gCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCtggcgtg                         <  1:326213/251‑1 (MQ=255)                                                                                                                                                                                                                          gtgaACGTCGCGTCTACGCGGGTAAGTTTTCCAATCGGTTCGCATGCACCCTGTCCCGGAACAGGCAGGGCAACCAGCGTGGCATGACGCAGGTAGTCTTTTACAGCGCCAGAAGGGCCAAACCACACGTCATCGTATTCACACGGACGTTGACGCGATAGCGAAGAAGACAGCGTTTCGTTACAACCAGAAGGAATTTTACACCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGcc               >  2:482333/3‑251 (MQ=255)                                                                                                                                                                                                                             aaCGTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCGCTGCCCTAATGCCTATGAATGCTGGCGTGGCGCAGTGCCTTc            >  2:555044/1‑251 (MQ=255)                                                                                                                                                                                                                                gTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACa                                                                                              <  2:553052/166‑1 (MQ=255)                                                                                                                                                                                                                                gTCGCATCTACGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACa                                                                                              >  1:553052/1‑166 (MQ=255)                                                                                                                                                                                                                                         aCGCGGGTCAGTTTTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACa  <  2:73977/249‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             CGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACA  >  NC_000913/1493983‑1494463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑