Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	848,229	A→T	intergenic (‑225/+179)	glnH ← / ← dps	L‑glutamine ABC transporter periplasmic binding protein/DNA protection during starvation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	848,229	0	A	T	100.0%	152.9 / NA	42	intergenic (‑225/+179)	glnH/dps	L‑glutamine ABC transporter periplasmic binding protein/DNA protection during starvation protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (25/17);  total (25/17)

CTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATAT  >  NC_000913/847986‑848475                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       cTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAACACTGATGAAATAACAATc                                                                                                                                                                                                                                                 >  2:517129/1‑251 (MQ=255)    ttAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCtt                                                                                                                                                                                                                                               <  1:583859/250‑1 (MQ=255)         aCAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTa                                                                                                                                                                                                                                         <  1:551472/251‑1 (MQ=255)                                      tCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGCGCCAGTTTTGCAACTCCTTGATTTTTCGAAATGAAGCAAAAAAGTGATGCACGCTTTCTAGGGCACTACACTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGAAAAACCTGATGAAATAACAATCTTTATGTAACGATTGAGTGATGATGTGGATACaaaa                                                                                                                                                                                                             >  1:649416/1‑250 (MQ=255)                                         aCGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAAtt                                                                                                                                                                                                         <  2:138889/251‑1 (MQ=255)                                                 aTCCCCTGCGTGACATTCACATATATGAAAAAATCGGGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAAt                                                                                                                                                                                                 <  2:623937/251‑1 (MQ=255)                                                  tCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAACTTTACTTCATc                                                                                                                                                                                                >  1:370000/1‑251 (MQ=255)                                                  tCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATc                                                                                                                                                                                                >  1:287044/1‑251 (MQ=255)                                                        gCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGGGTGATGATGTGGATACAAAAAATTTTCTTAAGCAGCt                                                                                                                                                                                            <  2:475305/249‑1 (MQ=255)                                                          gTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTATAGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAAACTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGgag                                                                                                                                                                                        <  2:21287/251‑1 (MQ=255)                                                           tGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTgg                                                                                                                                                                                          >  1:424974/1‑248 (MQ=255)                                                            gACATTCACATATATGAAAAAAGCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGAt                                                                                                                                                                                      <  2:592464/251‑1 (MQ=255)                                                                   acaTATATGAAAAAATCGTGCCAGGTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAgat                                                                                                                                                                               <  2:343658/251‑1 (MQ=255)                                                                       atatGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAgatggat                                                                                                                                                                           <  1:388143/251‑1 (MQ=255)                                                                          tGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGc                                                                                                                                                                         >  1:98630/1‑250 (MQ=255)                                                                                 aTCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATa                                                                                                                                                                 <  1:494142/251‑1 (MQ=255)                                                                                   cGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATa                                                                                                                                                                 >  1:306384/1‑249 (MQ=255)                                                                                                            ttttCAAGATGCGGCACAAACGTGATGCCCGATTTATAGGGCCATACCCTGAAGATACACCCTTCTGGGGCACCAATCTGGTGCGCTAACATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATCCCAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCCCTAAATAAGTGCGTTGAGGTGGGCTGcaaaaca                                                                                                                                       >  2:656417/1‑250 (MQ=255)                                                                                                             tttCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGcaaaacaa                                                                                                                                      >  1:22277/1‑250 (MQ=255)                                                                                                               tCGAGATGCGGCAAAAACGTGATGCTAGATTTATAGGGCAATCCCCTGAAGATGCACCATGCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTTATGAAATAACAATCTGTAGGTAACGATTGTGTGATGATGTGGATACAAAAATTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTTCGTGGAGGTGTGCTGcaactcaaaa                                                                                                                                    <  2:387126/250‑8 (MQ=255)                                                                                                               tCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCaaaacaaaac                                                                                                                                   >  1:247966/1‑251 (MQ=255)                                                                                                                           aaaaaCGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTc                                                                                                                        >  1:284488/1‑250 (MQ=255)                                                                                                                                cGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGa                                                                                                                    >  1:149263/1‑249 (MQ=255)                                                                                                                                   gATGCACGATTTATAGGGCAATACCCTGAAGATGCTCCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCg                                                                                                               <  1:523380/251‑1 (MQ=255)                                                                                                                                     tGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCt                                                                                                             >  1:166520/1‑251 (MQ=255)                                                                                                                                              tataGGGCAATACCCTGCAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCACCACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGCTTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCggg                                                                                                    >  2:362783/1‑251 (MQ=255)                                                                                                                                                  gggCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAACCAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACt                                                                                                >  1:650491/1‑251 (MQ=255)                                                                                                                                                   ggCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAACTAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACt                                                                                                >  2:538876/1‑250 (MQ=255)                                                                                                                                                      aaTACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGAGATTATTTAGCGTAAAACCTGATGAACTAACAATCTTTATGTAAAGATTGTGTGATGATGTGGATAAAAAAAATTTACTTAATCAGCTGGAGATAGAAGATGGATGCAATAAATAAGTGCGTTGAGATGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGACGATATTATCGGGTACTaa                                                                                              >  1:49219/1‑249 (MQ=255)                                                                                                                                                                  aTGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATc                                                                                >  2:217346/1‑251 (MQ=255)                                                                                                                                                                      aCCATTCTGAGGCACCAATCTGGTGCGCTAAAATCGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATAGGGTACTAAAGTTCTGCACCATCAGCg                                                                            >  2:119133/1‑251 (MQ=255)                                                                                                                                                                      aCCATACTGGGCCACCAATCTGGTGAGCTCAAATTGTACACTCACCACAGTGATTATTTACCGTAAAACCTGAAGACATAACAATCTCTATGTAACGTTTGTGTGCTGATGTGGATACAAAAACTATACTTAATCAGCTGAAGATAGAAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAATGTTATGCACCATCAGCg                                                                            >  2:373181/1‑251 (MQ=255)                                                                                                                                                                                           ggTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGAGTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGAGGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATGTATACGATGtt                                                          <  2:650491/248‑1 (MQ=255)                                                                                                                                                                                                                cAACACAGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAAttt                                  >  1:684314/1‑251 (MQ=255)                                                                                                                                                                                                                     caGTGATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATcc                              >  2:130878/1‑250 (MQ=255)                                                                                                                                                                                                                         gATTATTTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTc                         >  1:194848/1‑251 (MQ=255)                                                                                                                                                                                                                             attTAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCga                     <  1:480444/251‑1 (MQ=255)                                                                                                                                                                                                                              tttAGCGTAAAACCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTcgcg                      >  2:242352/1‑249 (MQ=255)                                                                                                                                                                                                                                      aaaaCCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTAGCCAGGTCGCGAGACgcggc             <  1:656417/250‑1 (MQ=255)                                                                                                                                                                                                                                       aaaCCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGTAATTTATCCAGGTCGCGAGACGcggcgg           <  2:258107/251‑1 (MQ=255)                                                                                                                                                                                                                                        aaCCTGATGAAATAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGt          >  1:624403/1‑251 (MQ=255)                                                                                                                                                                                                                                                gaAATAACAATCTTTATGTAACGATTGTGTGAGGATGTGGATACAAAAAATTTACTTAAGCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGatat  <  2:306384/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       CTTTTAATACAGACTTCATAGCATTTCCTTTTTTCTATCCACGGGACGATCCCCTGCGTGACATTCACATATATGAAAAAATCGTGCCAGTTTTGCAACTCCTTGATTTTTCGAGATGCGGCAAAAACGTGATGCACGATTTATAGGGCAATACCCTGAAGATGCACCATTCTGGGGCACCAATCTGGTGCGCTAAAATTGTGCACTCAACACAGTGATTATTTAGCGTAAAACCTGATGAAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGGAAGCCGCTTTTATCGGGTACTAAAGTTCTGCACCATCAGCGATGGATTTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGACGCGGCGGTCAGGATAT  >  NC_000913/847986‑848475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑