| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 164,396 | C→T | I764I (ATC→ATT) | hrpB → | RNA‑dependent NTPase HrpB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 164,396 | 0 | C | T | 96.9% | 208.2 / ‑0.9 | 65 | I764I (ATC→ATT) | hrpB | RNA‑dependent NTPase HrpB |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (26/37); total (27/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.51e-01 | |||||||||||
CATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG > NC_000913/164150‑164642 | catgtATCAGGCACTACGCGGATTACTTGCTTGGGGAATGCAGCAATGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAAGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGGGCTGGAGGTG‑CTTTCACCTTCCCAAAGGCCCTTACAAATTacac > 2:574011/5‑251 (MQ=255)cATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTacac < 2:292126/251‑1 (MQ=255) aTCAGGCACAACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCTCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGGTTGGCGAGGCCACCAAGCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCGCCTGCCCAAATGCCATTACAAATTACACgaga < 2:515809/250‑1 (MQ=255) gCACTACGCGGATTACTTGATTGGGGAATGCAGAAACGTCTGGCTAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACATGCCCAAAGGCCATTAAAAATTACACGTGATTTg > 2:645837/1‑249 (MQ=255) aCGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCTCCCAGGGGCGCTTGCCGCTGGTGCTTGAGTTT‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGAc < 2:447163/250‑1 (MQ=255) cgcgGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAGTTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGCCGATCGCCCAGGGGCGCGGGCCGCTGGTGCTGGCGTTG‑CTTTCACCTGCCCACAGGCCACTACCAATTACACGAGGTTTGAGCGACtt > 1:277318/1‑251 (MQ=255) ggATTACTTGATTGGGGAATGCCGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCTGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTg > 1:137998/1‑251 (MQ=255) ggATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTg < 1:13400/251‑1 (MQ=255) aTTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCTCATTACACTGGGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGCTAACCCGACCGCGCTGGCGGTGAGCATGCAAGAGAAGGTTGACGAGGCCACCAATCCGACGATCACCCAGGGGCGCGTGCCGCTGGGGCTGGAGTTG‑CTTTCACCTGCCCCAAGGCCATTACACATTACCCCAGATTTGAGCGACGTCTgg > 2:547239/1‑250 (MQ=255) aCTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGaaa < 1:183235/250‑1 (MQ=255) aCTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGaa > 1:253945/1‑249 (MQ=255) tGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGt > 1:29106/1‑250 (MQ=255) gggAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTAcc < 1:63051/251‑1 (MQ=255) gTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGGGCaaaa > 1:401658/1‑250 (MQ=255) aGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGa < 2:600976/250‑1 (MQ=255) aTTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATATCCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGc < 2:185629/251‑1 (MQ=255) gcATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATccc > 1:219728/1‑250 (MQ=255) gACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGCCTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACCTGTCTGGCCgg > 2:126031/1‑251 (MQ=255) cGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGCCGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGgac > 1:589282/1‑251 (MQ=255) cGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGgac > 1:72242/1‑251 (MQ=255) aGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCACGGGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTGCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTACCCGAAACATGTCTGGGCGGACGAccc < 2:277318/251‑1 (MQ=255) gCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCg < 2:29106/251‑1 (MQ=255) ccATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGAGTTCTGGAAAGGAGCGTACCGGGAGGTGTAAAAAGAGATGAAAGGGCGTTAGCCCAAACATGTCTGGCCGGACGACCCTGCAAAttct < 2:61284/251‑4 (MQ=255) ccacgacgctcttccgagcccgcccgCGCTGGCGGTGAGAAGGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGTCAAATACTGCAc < 2:489471/233‑1 (MQ=255) ttATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGAGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGCGATTTGAGCGACTTCTGGCAAGGAGCGTACCGAGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACcg > 2:274769/1‑250 (MQ=255) agcATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACcgacg < 1:694652/249‑1 (MQ=255) aTCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACcgac < 1:126031/250‑1 (MQ=255) cATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATTCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGAGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGGGATTTGAGCGACTTCTGGAAAGGAGCGCACCGTGAGGTGAAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGACGGACGACCCGGCAAATACTGCACcgacgcg > 1:679645/1‑251 (MQ=255) gagaaCCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGAGTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTGATCCCAAACAt < 2:120525/204‑1 (MQ=255) gATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCAACTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACAt > 1:120525/1‑207 (MQ=255) ccgcccgcGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGaaaaa < 1:645837/251‑1 (MQ=255) cgcccgcGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCAc > 1:489471/1‑231 (MQ=255) cGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAggt < 1:526460/251‑1 (MQ=255) gagaATGCAAGAGATGTTTGGCGAGGCCCCCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATGCGTAAggttgg < 2:471878/250‑1 (MQ=255) agaATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAggttggtt < 2:210804/251‑1 (MQ=255) aTGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAGACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtc < 1:687420/251‑1 (MQ=255) aagtgaggtgtGGCGAGGCCGCGCATTCGACGGTCGCCCAGGTGCGCGTGCGGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCGAGTATAAATTACACGAGAGTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGATATGAAAGGGCGGGATCCCAAACATGTCTGGCCGGACGACCCGGGATATGCTGTAGGGTCTCGACGGACGAAGAAGTATGCTTAAGGTTGGTTTtctccc < 2:622961/241‑1 (MQ=255) aaGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTTGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctccc < 1:675064/251‑1 (MQ=255) aaGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctccc < 1:536167/251‑1 (MQ=255) ttGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctccctctccatg < 1:574011/250‑4 (MQ=255) ggCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTgg > 2:345326/1‑251 (MQ=255) gAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGTACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGgag < 2:104253/251‑1 (MQ=255) gAGGCCACCAAGCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgag < 1:23787/251‑1 (MQ=255) aGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTCTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTggg > 2:227365/1‑248 (MQ=255) aGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgaga < 2:253945/251‑1 (MQ=255) caATCCGCCGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCgg < 2:343562/251‑1 (MQ=255) caATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCgg > 1:155808/1‑251 (MQ=255) ccGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAACGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTg > 2:139219/1‑251 (MQ=255) ccGCCCAGGGGCGTGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCAAAAGGCCATTAAAAATTACACGAGATTTGAGCGACTTCGGGAAAGGAGCGTACCGTGAGGTCCAAAAAGAGATAAAAGGGCGTTATCCCAAACATGTCTGGCTGGTCGACCCGGAAAATCCTGCACCGACGCGACGGAGGAAAAAGTATGCGTATGGTGGGTTTTCCCCCGCTCCCTGGGGGAGGGGGCCGGGGTGAGGGCAt < 2:60005/250‑1 (MQ=255) ggCGCGTTCCCCGGGTGCTGG‑GTTGGCTTTCACCTGCCCAAATGCCATTAAAAATTACACGAGATTTGGGCGATTTCGGGAAAGGAGCGTACCGTGGGGTGAAAAAAGGGAGGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCCCCGCCGCGGCGGACGAAAAAGTATGCGTAGGGTTGGTTTGCTCCCTCTCCCTGTGGGAGAGGGCCGGGTTGAGGGCATCAGCGCGCa < 2:476936/251‑1 (MQ=255) ggcgTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCAATTACAAATTCCACGAGATTTGAGCGCCTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACGGCACCGACGCGACGGACGAAAAATTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACg < 1:500473/250‑1 (MQ=255) ggAGTTC‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAc < 1:26237/251‑1 (MQ=255) gAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc > 1:669700/1‑251 (MQ=255) tttCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGCCTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGCGAGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCCCCCTCACCCTa > 2:404026/1‑241 (MQ=255) tttCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGGGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACAGGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCCCCCGGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTa < 1:404026/241‑1 (MQ=255) tCACCTGCCCAAAGTCCATAACAAATTAAACGAGATTTGAGCGACTTCTGGACAGGAGCGTACCGAGAGGTGCAAAAAGAGATGAAAGGGCGTTATCGCAAACATGTCTGGCCGGACGAACCGGCAAATACTGCACCGACGCGACGGACGAAAAAGAATTCGTAAGGTTGGTTTTCTACCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGAACGTTTACCCTCACCCTAACCCTCTCCCTc > 2:704794/1‑251 (MQ=255) tCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctccctctcc > 1:849/1‑185 (MQ=255) tCACCTGCCCAAAGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctccctctcc < 2:849/185‑1 (MQ=255) aaGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCaa < 2:517557/116‑1 (MQ=255) aaGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCaa > 1:517557/1‑116 (MQ=255) aGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa > 2:539155/1‑241 (MQ=255) aGGCCATTACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 1:539155/241‑1 (MQ=255) ttACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCACTGTGGGAGAGGGCCGGGGGGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGGGAGGGGACCGAt > 2:88801/1‑251 (MQ=255) tACAAATTACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGCCGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAt < 1:462020/250‑1 (MQ=255) aaaTTACAGGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATAAAAGGTCGTGATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCCCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 2:589282/251‑1 (MQ=255) | CATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTG‑CTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG > NC_000913/164150‑164642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |