Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	144,160	0	C	T	30.6%	76.0 / 18.0	49	F153F (TTC→TTT)	yadH	putative ABC transporter membrane subunit YadH
Reads supporting (aligned to +/- strand):  ref base C (3/31);  new base T (15/0);  total (18/31)
Fisher's exact test for biased strand distribution p-value = 5.18e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCATTATTGCCGGATATGTCGGCGGTGGCGTGGCGCGTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCGGTATCAATGATGTTCCGCTGGTCACTACCTTTGGCGTACTGG  >  NC_000913/143921‑144401                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          aaTGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCATTATTGCCGGATATGTCGGCGGTGGCGTGGCGCGTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCggg                                                                                                                                                                                                                                              <  1:280006/251‑1 (MQ=255)     ccTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCATTATTGCCGGATATGTCGGCGGTGGCGTGGCGCGTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCCTTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGGGTT‑GTTTT‑ccccttcggggttt                                                                                                                                                                                                                                           >  1:139926/1‑240 (MQ=255)          gCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCATTATTGCCGGATATGTCGGCGGTGGCGTGGCGCGTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGGGGT‑GTTTT‑CCCttatggggttgcttgg                                                                                                                                                                                                                                      >  2:164844/1‑237 (MQ=255)            cAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCATTATTGCCGGATATGTCGGCGGTGGCGTGGCGCGTGGTCTGTTTGTTGGCATTCTGGTGACGGCCATTTCACTGTTTTTTTTGCCCTTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑TTTTT‑TCCTT‑TCGGGttttcttgacg                                                                                                                                                                                                                                   >  1:568745/1‑243 (MQ=255)             aaCGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGCAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCATTATTGCCGGATATGTCGGCGGTGGCGTGGCGCGTGGTCTGTTTGTTGGCCTTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCTGGTGCATTCGTGGGTATTCGTT‑GCCTTTACGCTGGGGCTCACGGCGGTGGT‑GTTCc‑ccccttccggtttggtgtgagg                                                                                                                                                                                                                                   >  1:604314/1‑232 (MQ=255)                gTTGCGTCATCATTTGTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCTCCGGTTCCGACTCACGGTATTATTGCCGGATATGTCGGCGGGGGCGTTGCGCGTGGTTTTTTTGTTGTCATTCTGTTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGTTTCATTCTTGTTTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGgtgt                                                                                                                                                                                                                               <  1:703164/251‑1 (MQ=255)                  ggCGTCATCATTGTTTGGTGCCAAGTGCCAGCTGAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCGCGGTATTATTGCCGGATAAGTCGGCGGTGGCGTGGCGCTTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTGGTGCCAGTTCAGGTGCATTCGTGCGTATTCGTT‑GCCTTAACGTTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGgtgtg                                                                                                                                                                                                                              <  1:313672/249‑1 (MQ=255)                           atTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCATTAGTGCCGGATATGTCGGCGGTGGCGTGGCGCGTGGTCTGTGTGTTGGCATTCGGGTGACGGCAATTTCACTGTTTGTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCTTTTACGCTGGTGCGCACGGCGGTTTT‑GTTCG‑CCCGT‑GTGGGGTTGCGGAGTGGTGTGTCGGCCaaaa                                                                                                                                                                                                                    <  2:416013/251‑1 (MQ=255)                             tttttGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTATCGCCGGTTCCGACTCACGTCATTATGGCCGGTTATGTCGGCGGTGGGGTGGGGCGTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCCCTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTTACGCCGGTGTTCTCGGCGGTGTTTGTTTT‑CCCTT‑CTGGGTTTgtgtagcgtggtgtttttcgaa                                                                                                                                                                                                                     >  2:532767/1‑228 (MQ=255)                              ttttGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCATTATTGCCGGATATGTCGGCGGTGGCGTGGCGCGTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACg                                                                                                                                                                                                                  <  1:415177/250‑1 (MQ=255)                                                    aaTATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCATTATTGCCGGATATGTCGGCGGTGGCGTGGCGCGTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑cc                                                                                                                                                                                          <  1:383608/251‑1 (MQ=255)                                                         tGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCATTATTGCCGGATATGTCGGCGGTGGCGTGGCGCGTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AAcc                                                                                                                                                                                     <  1:501254/250‑1 (MQ=255)                                                          taaGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCATTATTGCCGGATATGTCGGCGGTGGCGTGGCTCGTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACAGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACTGTGTTTTTGCCAAAACGTTCGATGGCAGCAGCCTGGTG‑CC‑AACCt                                                                                                                                                                                    <  1:227691/249‑1 (MQ=255)                                                                               gTTCCGACTCACGTCATTATTGCCGGATATGTCGGCGGTTGCGTGTCGCGTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCTGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTT‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTa                                                                                                                                                             <  1:599942/251‑1 (MQ=255)                                                                                  ccGACTCACGTCATTATTGCCGGATATGTCGGCGGTGGCGTGGCGCGTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTAtt                                                                                                                                                           <  2:198849/250‑1 (MQ=255)                                                                                        cACGTCATTATTGCCGGATATGTCGGCGGTGGCGTGGCGCGTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGc                                                                                                                                                     <  1:456167/249‑1 (MQ=255)                                                                                                             gTCGGCGGTGGCGTGGCGCGTGGTCTGTTTGTTGGCATTCGGGTGACGGCAATTGCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGTGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑ATCCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑Cttt                                                                                                                            <  2:159923/251‑1 (MQ=255)                                                                                                                   ggTGGCGTTGCTCGTGGTCTGTTTGTTGGCATTCTGGTGCCGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTgcc                                                                                                                      <  1:299723/251‑1 (MQ=255)                                                                                                                   ggTGGCGTGGCGCGTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTACCGCTGGTGCTCACGGCGGTGTT‑GTTTT‑CCCTTTCCGGGTTT‑CTGAACGGGGTGTTTGCCCACACGCTCGCTGGCATTCTCCTCGGG‑CC‑CACCTTTTTGTT‑TACGCCCCTCCCCTATTT‑TGGCGGGGTCTTTTtctcccttaccttttttcc                                                                                                                        >  2:106868/1‑236 (MQ=255)                                                                                                                             cgcgTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTgg                                                                                                            <  1:49681/251‑1 (MQ=255)                                                                                                                             cgcgTGGTCTGTTTGTTGGCAGTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGCGTG‑GTTTT‑TCCCT‑GCGGGGTTGATGCACGGGGGGGTTGTCAAAAAGGTAGATGACATCAGCCCGGTG‑GC‑AACCTTTTTGGT‑AAAGCCAACCACGGATTT‑GGGCGGGGGGTTTTTCTCACT‑GC‑CTttggtggcccccttcttg                                                                                                            >  2:305333/1‑236 (MQ=255)                                                                                                                                gTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑TTTTT‑TCCTT‑TCGGGGTTTCTGGACGGGGTGTTTTTCAAAAAGTTCGATGACCTCCGCCTGGTG‑GC‑AACCTTTTTTTT‑TACGTCCCTCCCGGTTTT‑TGGCGGGGGGTTTTTCTCACT‑GT‑CTTTGTTTCCGCCGTTCttggc                                                                                                          >  2:470669/1‑246 (MQ=255)                                                                                                                                   gTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTTT‑TCCTT‑TCGGGGTTGCTTAACGGTGTGGTTGGCAAAACGTTCCGTGACATCAGCCTGGGG‑CCCAACCTTT‑TGTT‑AACGCCCCTCACGTATTT‑GGGGGGGGGTTTTTTCTTACT‑GA‑CTTTTTTGTCGCCGtttttgcagggg                                                                                                      >  2:608459/1‑241 (MQ=255)                                                                                                                                      tgtttgttGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGAGTT‑GTTCT‑CCCTT‑GCGGGGTTGGTGTACGGGGTGTTTGCCCAAACGTTCGATGGCATCAGCCTGGTG‑GC‑AACCTTTTTGTT‑AACGCCACTCCCGGATTT‑GGGCGGGGGCTTTTACTTACT‑GG‑CTTTGTTGCCGCCGGTCTGGCCAggggtg                                                                                                   >  1:511553/1‑248 (MQ=255)                                                                                                                                      tgtttgttGGCATTCTGGTGACGGCAATTTCACTGGTTTTTGTGCCATGTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTGCTGGCAAGGGCt                                                                                                    <  2:568745/250‑1 (MQ=255)                                                                                                                                      tgtttgttGGAATTCTGGCGACGCCAATTTCAATGTTTTTTGTGTCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGCGGT‑TTTTT‑TTCTT‑GCGGGGTTGCGTCACGGGGTGGTTGCCAAAACCGTCTCTGAAATCACCCTGGGG‑GG‑AAACATTTTGTT‑AACGCCACTCCCCTCTTT‑GGGAGGGGTCTTTTTATCACT‑CA‑CTTTTTTGGCGCCGTTCttgaagaggctg                                                                                                   >  2:267060/1‑240 (MQ=255)                                                                                                                                       gtttgttGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑TCCTT‑TCGGGGTTGCTGAACGGGGTGTTTGCCAAAAAGGTTGATGACATCAACCTGGGG‑GC‑AACCTTTGTGGT‑TACGCCCCTCACGAATTT‑GGGCGGGGTCTTTTTCTTACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGgtgt                                                                                                  >  1:188091/1‑251 (MQ=255)                                                                                                                                         ttgttgGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGCTGTc                                                                                                 <  1:71159/250‑1 (MQ=255)                                                                                                                                                      ggTGACGGGTATTTCACTGTTTTTTGGGCCATTTCGGGTGCATTCGTGGTGATTCGTT‑GCCTTAACGTGGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTG‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCGGGTG‑CC‑AACCGTTGTGTG‑AACGCCACTCACGTATTG‑GGGCGGGGTCTTTTACGCACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCa                                                                                    <  2:63062/250‑1 (MQ=255)                                                                                                                                                       gTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTTT‑CCCCT‑TCGGGGTTGTTGAAAGGGGGGTTTTCCCAAACGTTCGATGACCACAGACCGGTG‑CC‑AACCTTTGTGTT‑TACCCCCCTCCAGTATTT‑GGGGGGGGGCTTTTTTTCACT‑GTTCTTT‑TTTCCACCCTTCTGGCAACGGCTGTAGCAcccgtaccccct                                                                                  >  2:455054/1‑241 (MQ=255)                                                                                                                                                            ggCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGttt                                                                             <  1:608459/251‑1 (MQ=255)                                                                                                                                                                    cACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTTT‑CCCCT‑GCGGGGTTGCTTTACGGTGGGTTTGCCCAAACGTTCGATGACAACCGCCTGGTGGCC‑ACCCTTT‑TGTT‑AACACCACTCAAGTATTT‑TGGGGGGGTATTTTATTTACTTGA‑‑TTTGTTTCCGCCGTTTTGGGCAGGGCTGTCCCACCTGAAccccctcggttatgttatc                                                                     >  2:135666/1‑235 (MQ=255)                                                                                                                                                                    cACTGTTTTTTGTGCCATTTCAGGGGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGTTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGTTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAGCCCAATCGTTTATATGATc                                                                     <  1:613070/251‑1 (MQ=255)                                                                                                                                                                          tttttGTGACATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTTTT‑TTTTT‑TCCTT‑TCTGGTTTTCTTATCGGTGTTTTTGCCCAAAAATTCTCTTACATTAGCCTGGTG‑CC‑AACCTTTTTTTT‑AAACCCACTCCAGTATTTTGGGGGGGGTTTTTTA‑TCTCT‑TT‑CTTTTTTTCCGCCGTTCTGGGAAAGGCTGTTGCCCCTGGACCCCAACGTTtattttgtaagtggt                                                               >  1:653619/1‑239 (MQ=255)                                                                                                                                                                               gtgCCATTTCAGGTGCAGTCGTGGGTAT‑CGTTTGCCTTACGGGTGGTGCTCACGAGGGTGTT‑GTTCT‑CCCTT‑GGGGGTTTGGTGACCGGTGTGTTTGCCAGAACGTTCGATGTCATCAGCCTGGAG‑CC‑AACCTTTGTGTT‑AACGCCGCTCACGTATTT‑GGGCGGGGACTTTAACCCACT‑GA‑CTTTGTTGCCGGCGTTCTGGGAAGTGCTGTCGCTCCTGAACCCAATCGTTTATATGATCAGTGGTTTcc                                                           <  2:511553/250‑1 (MQ=255)                                                                                                                                                                                    aTTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGGT‑GTTTTTCCCTT‑‑CGGGTTTGCTGAACCGTGGGTTTGCCAAAACGTTTTGTTACATTAGCCCCGGG‑CC‑CACCTTTGTGTTTACCGCCAC‑CCCCTATTT‑TGGCGGGGGCGTTTTCTCACT‑GA‑CTTTTTTGCCCCCGTTCTGTCAAGGGCTGTCGTCCCTCAACCCCATCGttttattaatacgtgggtttcgctact                                                     >  2:557567/1‑227 (MQ=255)                                                                                                                                                                                        cAGGTGGATTCGTTGGTATTCGTT‑GCCTTATCGCTGGGTCTCCCGGCGGGGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGTACGTTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTTTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCt                                                  <  2:647899/250‑1 (MQ=255)                                                                                                                                                                                         aGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTc                                                <  1:573030/251‑1 (MQ=255)                                                                                                                                                                                          ggTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTcc                                               <  1:194764/251‑1 (MQ=255)                                                                                                                                                                                             gCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCg                                            <  1:106868/251‑1 (MQ=255)                                                                                                                                                                                                        tATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GGTTT‑TCCTT‑TCGGGTTTGGTGAAAGGGGTGTTTGCCAAAACGTTCGATGAAATCAGCCTGGTG‑CC‑AAACTTTGTGTT‑TAAACCCCTCACGATTTT‑GGGCGGGGGCTTTTACTCAAT‑GA‑CTTTGTTGGCGCCGTTATGGGAAAGGCTGTTGCCCCTGAACCCAATCGTTTATATGATAAGGGGTTTCCGCGTCGGGTTCCTCgggattaataat                                 >  1:142657/1‑241 (MQ=255)                                                                                                                                                                                                                 gCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTTCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCGGTATCAATGATGTTCCGc                          <  1:228506/250‑1 (MQ=255)                                                                                                                                                                                                                  ccTTAACGCTGGTGCTCACGGCGGTGTT‑TTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCGGTATCAATGATGTTCCGCTg                        <  2:411354/251‑1 (MQ=255)                                                                                                                                                                                                                   cgtatcGCTGGTGCTCACGGCGGTGTT‑TTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACTGTCGATGTCATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGCTTTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCGGTATCAATGATGTTCCGCTgg                       <  2:677180/246‑1 (MQ=255)                                                                                                                                                                                                                        cGCTGGTGCTCACGGCGGTGTT‑GGTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGTCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTTGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCGGTATCAATGATGTTCCGCTGGTCACt                  <  1:456575/251‑1 (MQ=255)                                                                                                                                                                                                                         gCTGGTTCTCACGGCGGTGTT‑GGTTT‑CCCTT‑GCGGGGTTGCTGACCGGGGGGGTTGCCCCAACGTTCCATGCCATCAGCCTGGGG‑CC‑CACCTTTGTGTT‑AACGCCCCTCACGAATTT‑GGGCGGGGTCTTTTCCTCCCT‑GA‑CTTTTTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTCTACCCAATTGTTTATATGATCAGTGGGTTCCGCGACGGCTTCCTCGGTAACAATGATGTTTCGCTGGGCACtc                 >  2:442042/1‑250 (MQ=255)                                                                                                                                                                                                                         gCTGGTGCTCACGGCGGGGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCGGTATCAATGATGTTCCGCTGGTCACTa                 <  2:541600/251‑1 (MQ=255)                                                                                                                                                                                                                                  cACGGCGGTTTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCTCTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCGGTATCAATGATGTTCCGCTGGTCACTACCTTTGGc         <  1:420391/250‑1 (MQ=255)                                                                                                                                                                                                                                        ggTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCGGTATCAATGATGTTCCGCTGGTCACTACCTTTGGCGTACtgg  <  2:553543/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          AATGCCTACGCCAACGTTGCGTCATCATTTTTTGGTGCCAAGTTCCAGCGTAATATTGAAGAGCTGCTGGTAGCGCCGGTTCCGACTCACGTCATTATTGCCGGATATGTCGGCGGTGGCGTGGCGCGTGGTCTGTTTGTTGGCATTCTGGTGACGGCAATTTCACTGTTTTTTGTGCCATTTCAGGTGCATTCGTGGGTATTCGTT‑GCCTTAACGCTGGTGCTCACGGCGGTGTT‑GTTCT‑CCCTT‑GCGGGTTTGCTGAACGGTGTGTTTGCCAAAACGTTCGATGACATCAGCCTGGTG‑CC‑AACCTTTGTGTT‑AACGCCACTCACGTATTT‑GGGCGGGGTCTTTTACTCACT‑GA‑CTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCGGTATCAATGATGTTCCGCTGGTCACTACCTTTGGCGTACTGG  >  NC_000913/143921‑144401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑