| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,851,654 | 0 | T | G | 30.8% | 114.8 / 11.3 | 68 | G218G (GGT→GGG) | hypB | hydrogenase isoenzymes nickel incorporation protein HypB |
| Reads supporting (aligned to +/- strand): ref base T (11/34); new base G (19/1); total (33/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.85e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGTTATTGAAGGCGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACG > NC_000913/2851404‑2851903 | ccgttttttaaGGCGACCAGCAAACCGTGAACGATGCCGCCCGAATTCGCTCTACCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCGGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCGGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGt < 2:380676/242‑1 (MQ=255) aaGGCGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCAGTGAAAAACACAAAGAGGCGG‑GGCTTTTCG‑TTACCG‑CA‑GGAGAAGACaa > 1:598700/1‑250 (MQ=255) ggCGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑CA‑GGGGAAGACAAAcc > 1:74605/1‑251 (MQ=255) gACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑t < 1:501413/251‑1 (MQ=255) gACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAA‑CAACGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑CGCCTTCCG‑TTTCCG‑AA‑AGGGAAGaacaaccccc > 1:447659/1‑242 (MQ=255) ccAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑GGGTTTTCG‑TTTCCG‑CA‑GGGGGAGACAAACCAC‑Tg > 2:564897/1‑250 (MQ=255) aaaaCCGGGAACGAGGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAA‑CCCCGGTAAAGGCTGCCATCTTGACGCCCAGATGATTGCCGGCGCCGCCCCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAata < 2:549906/250‑1 (MQ=255) aCCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCCCCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCTTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCCC‑TGAAATATcc < 2:3388/251‑1 (MQ=255) ggaccGATGCCGCACGCATTCGCGCTCCCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGAGTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCat < 2:278221/247‑1 (MQ=255) aCGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATg < 1:25559/251‑1 (MQ=255) cGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑AGGGAAGACAAAACAC‑TGAAataaacccatatgt > 2:273258/1‑240 (MQ=255) gCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TTAAATAACCGCATATtttttc > 1:615119/1‑249 (MQ=255) ccGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTgcc < 2:599837/251‑1 (MQ=255) cgcgCTACCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTTCCC‑AA‑GGGGAAGACAAACCAC‑TGGAATATCCCCCTTAGTTTgccgcccccccccc > 1:690089/1‑243 (MQ=255) gcgcTACCGGCACACCAGCGATTCCGGCGAC‑CACCGGTAAAGGCAGCCATCTTGACGCACCGATCATTGCCAACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAACGTGGCGG‑CGCTTTCCG‑TTACAG‑AC‑GGTGAAGACAAAACAC‑TGGAAAAACCGCATATTTTTCTCGCCGCCACGCTg > 2:621730/1‑251 (MQ=255) gcgcTACCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTGTGCCGCCGCCTCGCTg < 1:574784/251‑1 (MQ=255) cgcTACCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGCCGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑a < 1:456482/251‑1 (MQ=255) ccccaCCGGCACCCCAGCGCTGC‑GGTGAAACACCGCTAAAGGCGACCATCTGGGCTCACAGATGATTGTCGATGCCGCACCGCGTCTGCCACTGGACGATAACGGTATGCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCTG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATCTGTTTgccgccgcccctctga < 2:213569/247‑8 (MQ=255) cTACCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTTCG‑TTTCCG‑AA‑GGGGGAGAAAAAACAC‑CGACAAAACCCCAAATGTTTTCCGCCGCCTCGccggtt > 2:667169/1‑246 (MQ=255) cacCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTGCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTcaac < 1:132332/251‑1 (MQ=255) gTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGtt < 1:477363/251‑1 (MQ=255) ccGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTAt < 1:656353/251‑1 (MQ=255) tAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTATCG‑AA‑GGGGGAAACAAACCCC‑TGAAAAATCCCCATATGTTTGCCGCCGCCTCGCTG‑GAGCTGCTCACCA‑AAGTTGA‑CCTGTTGCCGTAtctc > 1:268032/1‑250 (MQ=255) tAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑CA‑GGGGAAGACAAAACAC‑TGAAATATCCGCATATGTTTTCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTTCCGTAtatcc > 1:66738/1‑247 (MQ=255) aaaGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑aa < 1:564897/251‑1 (MQ=255) aaaGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑a < 2:615119/250‑1 (MQ=255) aaaGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAAAGGTAATCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGGTGCTTACC‑‑TTACCG‑AA‑GGTGAAAACAAAACACAGGAAAACCCCCCAT‑TGTTTTCCGCCGCCTCGGTG‑ATGACGCTCAACA‑AAAATGA‑CCttgtgccggaattcc > 1:483413/1‑236 (MQ=255) cGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGt < 2:74605/251‑1 (MQ=255) gatgatTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCg < 1:667169/251‑1 (MQ=255) cgccgcACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCCGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTTCCG‑AA‑AGTGAAGACAAACCCC‑TGAAATAAACGCCTATGTTTTCCCCCCCCTCGCTG‑ATGCTGATCCACA‑AAGTTGT‑CCTGTTGACGTCTCTC‑CAC‑TTTTACGGTGTAAAGTGCATAGCCTGCGCCcgcc > 1:595491/1‑250 (MQ=255) cgccgcACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTTCG‑TTACCG‑AA‑GGGGAAGACAAAACAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATTCTGCTCAACA‑AAGGTGA‑CCTGTTGCCGTATCTC‑AAA‑TTTGACGTTGAAAAGGGCATCGcccgcgcccgc‑g > 1:400540/1‑251 (MQ=255) cACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGACAAACACAAAGTGGCGG‑TGCTTTCCG‑CTTCCG‑AA‑GGGGAAGACAAACCAA‑TGAAAAATCCGCCTATGGTTTCCCCCGCCCCCCTG‑ATGCTGCTCAAAA‑AAAGTGA‑CCTGTTGCCGTATCTC‑CAC‑TTTGGCGTTGAAAAATGGATCGCCTCGGCCcgc‑gcagcc > 2:88250/1‑246 (MQ=255) cccGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAGGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCa < 1:635061/250‑1 (MQ=255) gTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTTCC‑TTTCCG‑AA‑GGGGAAGAAAAACCAC‑TGAAATATCCGCATATGTTTTCCGCCGCCCCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑ACTGTTGCCGGATCTC‑AAC‑CTTTACGTTGAAAAATGCCTCGCCCCCGCCCGC‑GC‑AGTAAAtacc > 2:556567/1‑248 (MQ=255) gCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGGTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑a < 2:196059/249‑1 (MQ=255) cGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAg < 1:570244/208‑1 (MQ=255) aTAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAatcatc < 2:22932/251‑1 (MQ=255) tAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGGGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCCCTG‑GTGCTGCTCAACA‑AAAGTGA‑CCTGTTGCCGTAACCC‑AAA‑TTTGACGTTGAAAAGGGCATCGCCTGCGCCCGC‑GA‑AGTCAAACCAG‑AAATTGAAATCATcc > 1:185219/1‑251 (MQ=255) cGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTTCG‑TTACCGGAA‑GG‑GAGGAAAAACAAC‑TGGAAAATCCGCATATGTTTGCCGCCGCCCCGCTG‑ATGTTGCTCAAAA‑AAATTGA‑CCTGTTGCCGCTTCTC‑AAA‑ATTGACGGTGTAAAGTGAATCTACTGGCCCCGC‑GA‑AGTCAAACCAG‑AAATTGAAATCATACtt > 2:223192/1‑250 (MQ=255) ggTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑GGCTTTCCG‑TTACCG‑AA‑GGGGGAGACAAACAAC‑TGAAATATCCGCATATTTTTGCCGCCCCCTCGCTG‑ATGCTTCTACACA‑AAGATGA‑CCTGTTGCCGGATCTC‑CAA‑TTTTACGTTGAAAAGGGCATCGCCTGCGCCCCCCGA‑GGT‑AATCCAG‑AAATTGAAAACATACTTAt > 1:422779/1‑251 (MQ=255) tCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTcc < 1:482459/250‑1 (MQ=255) tATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑CTTCCG‑AA‑GGGGAAGACAAACCAC‑TGCAATATCCGCATCTGTTTGCCGCCGCCCCGCTG‑ATGCTGCTCAACA‑AAAGTGA‑CCTGTTGCCGTATATC‑CAC‑TTTTACGTTTAAAAAAGAATCGCCCTCGCCCGC‑GC‑AGTCACACCAG‑GAATTTAAAACATCCTTATTTTCGcccccc > 2:103182/1‑247 (MQ=255) tATCGAAAACGTTGGCAACCTCGTATGCCCGCCCCGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑AACTTTCCGGTTACCG‑AA‑GG‑GACAACAAACCAC‑AGCAATATACCCATATGTTTGCCGCCGCCTCGCTGGAT‑CTGCTCAAAA‑AAAATGA‑CCCGTTTCCGTATCTC‑CAC‑TTTGACGTTGGAAAAAGCACCGCCCGCGCCCGC‑GA‑CGTCAAACCAGCAAATT‑AAAACTTCCTTCTTTCCGccacc > 1:134744/1‑250 (MQ=255) tCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGAGG‑AGCTTTTCG‑GTACCG‑AAAGGGAAAAACAAAC‑AC‑TGAAATATTCCCATATGTTTGCCCCCGCCTCGCCG‑ATGCTGCGCAACC‑AAGTTGA‑CCTGTTGCCGTATCTCCAAT‑TTTATGGTTGAAAA‑TGCATCGCTCGCGCCCGC‑GAGAG‑CAATCCCG‑AAATTTAAATCATCCTTATTTCTGccaccacc > 2:435068/1‑249 (MQ=255) cGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGGCAATCCAG‑AAATTGAAAGCATCCTTATTTCCGCCACCAgcg < 2:690089/251‑1 (MQ=255) aaCGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCC‑TTACCG‑AA‑GGGGAAGACAAACACC‑TGGAATATTCCCATATTTTTGCCGCCGGCTCGCTG‑ATGCTGCTCAACA‑AAGTTTG‑CCTGTTGCCGCATTTC‑CAC‑TTTTACGGTGAAAAGTGCATCGCCTGCCCCCCC‑CG‑AGTCAATACAG‑CAATTGAAATCATCCTTTTTTcctcccacaacggcg > 1:662061/1‑237 (MQ=255) cAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGAGGCGG‑TGCTTTCCG‑TTACCC‑AA‑GGGGAAAACAAACCAC‑AGAAAAATACGCATATGTGTGCCGACGCCTCGCTG‑CTGCTGCTCC‑CACAAGTTGAACCT‑TTTCCGGAACTC‑AAC‑CTTGACGTTGGAAAATGGATCGCCCGCGCCACC‑GA‑AGTCCATCCCG‑AAATTGACAACATCCATAttttcaccaaccgctgagcgaggatgg > 2:547768/1‑227 (MQ=255) cAACCTCGTATGCCCGGCCAGCTTCGAGCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATgg < 2:486582/251‑1 (MQ=255) aTGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTGCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTT‑ATGCTGCTCAACA‑AAGTGGA‑CCTGTTGCCGTATCTC‑AGC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAAGCATCCTTAGTTCCGCCTCCAGCGGCGAAGGGATGGACCAGTgg < 2:447659/250‑1 (MQ=255) aTGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑GGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTAGCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGAGGGACCAGTgg < 2:346845/250‑1 (MQ=255) gCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCt < 1:79400/250‑1 (MQ=255) ggCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTTA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAAct < 1:625985/251‑1 (MQ=255) gCCAGCTTCGATATCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTCCCC‑AA‑GGGGAAGACACACAAC‑CGAAATATCCGCCTATGTTTGCCGCCGCCTCGCTG‑AAGCTGCTCAACA‑AAATTGA‑CCTGTTGCCGTATCTC‑ACCATTTG‑CGTTGGAAAGGGCATCGCCCGCGCCCCC‑GA‑AGGAAATCCCA‑AAATTGAAATCATCCTTATTTCCGCCCCCACCGGGGAGGGGATGGGCCAGTggagggaccg > 2:272982/1‑243 (MQ=255) gATCTCGGTGAAAAACACAAAGTGGCGG‑TGGTTTTCG‑TTACCG‑AA‑AGGGGAGAAAAACCAC‑TGAAATATCCGCCTATGTTTGCCGCCCCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑CAC‑TTTTACGTTGTAAAGAGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCCTATTTTCGCCACCAGCGGCCGAGGGATGGACCAGTGGCTGTACTAGCTGgaga > 1:494851/1‑250 (MQ=255) cGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGc < 1:448932/251‑1 (MQ=255) cGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGc < 1:163214/251‑1 (MQ=255) aaaaaCACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGAtgt < 1:508702/251‑1 (MQ=255) aaaCACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCt < 1:33704/185‑1 (MQ=255) aaCACAAATTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGTAGACACAGCGATGTGCa < 2:406897/251‑1 (MQ=255) ggCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTcc < 1:451969/251‑1 (MQ=255) gg‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑ACC‑TTTGACGTTGAACAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCCGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGCTGTGCATAGGCGTTCCCgg > 1:687635/1‑251 (MQ=255) gg‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCACCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCgg > 1:134488/1‑251 (MQ=255) gg‑TGCTTTCAG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGGGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGAGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCCTAGGCGTTCCCgg > 2:485907/1‑251 (MQ=255) ttCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCAC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGcc > 1:581947/1‑247 (MQ=255) tCCG‑TTACCG‑AA‑GGGGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATGGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTACCCGGCCAGAt < 2:687635/250‑1 (MQ=255) cG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCg < 1:272982/251‑1 (MQ=255) g‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGGTCCCGGCCAGATCCGc > 1:249180/1‑251 (MQ=255) g‑AA‑GGTGAAGCCAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCAtt > 2:109105/1‑251 (MQ=255) gTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAACAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGCGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACg > 2:202335/1‑251 (MQ=255) | CAGTTATTGAAGGCGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAA‑CACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGG‑TGCTTTCCG‑TTACCG‑AA‑GGTGAAGACAAACCAC‑TGAAATATCCGCATATGTTTGCCGCCGCCTCGCTG‑ATGCTGCTCAACA‑AAGTTGA‑CCTGTTGCCGTATCTC‑AAC‑TTTGACGTTGAAAAGTGCATCGCCTGCGCCCGC‑GA‑AGTCAATCCAG‑AAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACG > NC_000913/2851404‑2851903 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |