Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,360,780	0	T	A	28.9%	78.1 / 10.6	45	L43F (TTA→TTT)	puuA	glutamate‑putrescine ligase
Reads supporting (aligned to +/- strand):  ref base T (18/14);  new base A (0/13);  total (18/27)
Fisher's exact test for biased strand distribution p-value = 4.35e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCTCCAGG‑TAG‑CGTTTCACT‑TCCTGC‑GTAAA‑GGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAAT  >  NC_000913/1360540‑1361011                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               ggTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACAACCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCCTTTAAATCGGTAAGTAAAA‑CATCa                                                                                                                                                                                                                                      >  2:534453/1‑250 (MQ=255)        aGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTg                                                                                                                                                                                                                             <  1:579597/251‑1 (MQ=255)         gCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGc                                                                                                                                                                                                                            <  1:588227/251‑1 (MQ=255)          cATCTGACCGATAAACGCTAGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGGTCCGGATCGCCCATTTCGTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATGCCGAGGCCGGGAAATAACGCCCTTTCT‑CGGGCTTCTTCAGGCTTGAAACA‑GGAATACGCATTCCACGG‑AA‑GCAACCATGTAAAGCGGTAAGTAAAA‑CATCAAC‑ATATTGCg                                                                                                                                                                                                                           <  1:37202/251‑1 (MQ=255)           aTCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGt                                                                                                                                                                                                                          >  1:74971/1‑251 (MQ=255)               gACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGt                                                                                                                                                                                                                          <  2:229293/247‑1 (MQ=255)                  cGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCggg                                                                                                                                                                                                                   >  2:235567/1‑251 (MQ=255)                   gATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGt                                                                                                                                                                                                                  <  1:311655/251‑1 (MQ=255)                              gATCAGCGGCAGAAAGAGTTAATGAACCGCGAACAGGAACTCAGTTACGATCCGTCTCGCCCAATTCCTGACCCAGACCTGCTTCTTCTACTACTTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTTAAACA‑GGAATACGCTTTCCACGG‑AA‑TCAACCAGTTAAAGCGGGAAGTAAAA‑CATCAGC‑ATATTTCGTATGCGGGTCGCGCTCCat                                                                                                                                                                                                        <  2:27615/250‑2 (MQ=255)                                      gcAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCTCCAGG‑TAG‑CGttt                                                                                                                                                                                             <  1:167761/251‑1 (MQ=255)                                                           agaACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCTCCAGG‑TAG‑CGTTTCACT‑TCCTGC‑GTAAA‑GGCGCt                                                                                                                                                                     >  2:343709/1‑251 (MQ=255)                                                              aCAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCGTCTCCTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCTCCAGG‑TAG‑CGTTTCACT‑TCCTGC‑GTAAA‑GGCGCTAcc                                                                                                                                                                  <  1:114025/251‑1 (MQ=255)                                                               cAGGAACACAGGTACGCTCCCGCTCGCAAATCTCCTGCCCCAGTCCTGACTCTTCCACTACCTTGCCCAGACTATCCATCACAAATACCGAGGACGGGAAATACCACCCTTTCT‑CGCCCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCCCGG‑AA‑GCAACCATTTAAATCGGTAAGTCAAA‑CATCAAC‑ATATTGCGTATTCCGGTAGCGCTCCAGC‑TAG‑CGTTTCAAT‑TACTGA‑GTAAA‑GGAGCTAccc                                                                                                                                                                 >  2:367696/1‑251 (MQ=255)                                                                       caGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCTCCAGG‑TAG‑CGTTTCACT‑TCCTGC‑GTAAA‑GGCGCTACCCCGCctctc                                                                                                                                                         <  1:83431/251‑1 (MQ=255)                                                                                                      ccAGACCTGCTTCTTCTTCTACGTTGCCAAAAATACCCATTGCAAATCCCGAGGCGGGAAAAAAACCCCTTTTCC‑CGAGCTTCTTAAGGCTTAAAACA‑GGAATACGCTTGCCAGGG‑AA‑GAAACAATTTAAATCGGTAAGTAAAA‑CACCAAA‑ATATTGCGTATTGGGGAAGCGCCCCGGG‑TGG‑CTTTTCCCT‑TCCTGC‑TAAAA‑GGCGCTACCCCGCCTCTCTGCTGACTGCTGAACAAAGTTCTCTACTTCa                                                                                                                          <  1:222650/251‑1 (MQ=255)                                                                                                           ccTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCTCCAGG‑CAG‑CGTTTCACT‑TCCTGC‑GTAAA‑GGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGat                                                                                                                     >  1:676121/1‑251 (MQ=255)                                                                                                                          acgttccacAGAATATCCATTGCAAATACCGAGGCCGGAAAATAACCCCCTTTCG‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTCCCACGG‑AA‑GCAACCATTTAAACCGGTAATAAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCCCCAGG‑TAG‑CTTTTCCCT‑TCCTGC‑GTAAA‑GGCGCTCCCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGAt                                                                                                      <  1:156295/243‑1 (MQ=255)                                                                                                                                cccAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCTCCAGG‑TAG‑CGTTTCACT‑TCCTGC‑GTAAA‑GGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCg                                                                                                >  2:45608/1‑251 (MQ=255)                                                                                                                                  caaaaTACCCATTGCAAAGCCCGGGGCGGGAAAATAACCCCCTTCCT‑CGAGCTTCTTCAGGCTTAAAACA‑GGAATTCGCTTCCCAGGG‑AA‑CCAACCATTTAAATCGGTAATAAAAA‑CATCAAA‑ATATTGCGTATGGGGTTAGCGCGCCGGT‑TAG‑CTTGTCACT‑‑CCTGCCGTAAA‑GGCTCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGcc                                                                                              <  1:426842/248‑1 (MQ=255)                                                                                                                                     aaTATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCTCCAGG‑TAG‑CGTTTCACT‑TCCTGC‑GTAAC‑GGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTTGTTTCCATGATTCTTCGCCttt                                                                                           >  1:76180/1‑251 (MQ=255)                                                                                                                                             gtGCAAATACCGCGGCCGGGAAATAACACACTTTCT‑AGAGTTTCATCAAGCTTGAAACA‑GGCATACGCTTTGGACGG‑AA‑GCAACCATTTAAATCGGTACATAAAA‑CAACAAC‑ATATTGCGTATTCGGGGGGGGCTACAGG‑TAG‑CGTTTCACT‑TCCTGC‑GTACA‑GGCGCTACCCCGCCTCTATTAGGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTAGCCTTTGgtttgtt                                                                                   >  2:690280/2‑251 (MQ=255)                                                                                                                                                      ccGAGGCGGGAAAAAAACCCCCTTCCT‑CGGGCTTTTCCAGGCT‑AAAACAAGAAATACGCTTCCCACGG‑AA‑GAAACCATTAAAACCGGAAAGTAAAC‑CTCCAAC‑AGATTGCGTATTCGGGTAGCGC‑CCAGGGTGG‑CGTTCCTCT‑TCCTGC‑GTAAA‑GGCTCTACCCCGCCGCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCg                                                                          <  1:233052/251‑1 (MQ=255)                                                                                                                                                        gAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCTCCAGG‑TAG‑CGTTTCACT‑TCCTGC‑GTAAA‑GGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGtt                                                                        >  1:60558/1‑251 (MQ=255)                                                                                                                                                         aGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCTCCAGG‑TAG‑CGTTTCACT‑TCCTGC‑GTAAA‑GGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGtt                                                                        >  2:110403/1‑250 (MQ=255)                                                                                                                                                            ccGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCTCCAGG‑TAG‑CGTTTCACT‑TCCTGC‑GTAAA‑GGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATc                                                                     >  2:306426/1‑250 (MQ=255)                                                                                                                                                               gaaaaTACCACCCTCTCC‑CGAGCGTCTTCAGGTTTAAAAAA‑GGAATACGTTTTCC‑CGGTAA‑GAAACAATTAAAATGGGTAAGTAAAA‑AATAAAA‑ATATTGGTTATGCGGGTAGCGCCCGAGG‑TAG‑CGTTTC‑CTCTCTTGT‑GAAAA‑GGCGATACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGTCTTTGGTTTGTTTGCCGCTCGTTATCAAAg                                                                 <  1:604716/249‑1 (MQ=255)                                                                                                                                                                  aaTAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCTCCAGG‑TAG‑CGTTTCACT‑TCCTGC‑GTAAA‑GGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGAttcttc                                                                                                 >  1:575595/1‑216 (MQ=255)                                                                                                                                                                         cccTTTCTTCGTGCTTCTTCAGGCTAAAAAAA‑GGAATGCTCTC‑CCCCGG‑AAAGAAACCATTAAAATGGGTAATAAAAA‑CAACAAC‑ATATTGCGTATGGGGGTACCGCCCCGGG‑TAG‑CGTTTCACT‑TCCTGC‑GTAAA‑GCCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAata                                                        <  1:237339/251‑1 (MQ=255)                                                                                                                                                                                 ccGAGCTTCTTAAGGCTTGAAACA‑GGAATCCCCTTTCCAGGA‑AA‑CCAACCATTTAAATCGGAAAAAAAAC‑CATCACC‑ACATTGCGTATTCGGGTACCCCCCCTGG‑TAG‑CGTTTCTCT‑CCCTGC‑TAAAA‑GGCGCTCCCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGacc                                                <  1:339222/250‑1 (MQ=255)                                                                                                                                                                                    aGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCTCCAGG‑TAG‑CGTTTCACT‑TCCTGC‑GTAAA‑GGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGaccacc                                             >  1:548189/1‑251 (MQ=255)                                                                                                                                                                                          ttcAGGCTTGCACCA‑GGACTACGCTTTCCACGG‑AC‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGATAGCGCTCCAGG‑TAG‑CGTTTCACT‑TCCTGC‑GTAAA‑GGCGCTACCACGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCg                                        >  2:611061/1‑250 (MQ=255)                                                                                                                                                                                            cAGGTTTGAAACA‑GAAATCCTCTTCCCACGG‑AA‑GCAACCATTTAAATCGGTAAGAAAAA‑CATCAAA‑ATATTGCGTATTCGGGTAGCCCTCCAGT‑TAC‑CGTTTCCCT‑TCCTGC‑GAAAA‑GGCCCTCCCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAAt                                     <  1:343709/251‑1 (MQ=255)                                                                                                                                                                                            cAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCTCCAGG‑TAG‑CGTTTCACT‑TCCTGC‑GTAAA‑GGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCttt                                                                                           >  1:474685/1‑197 (MQ=255)                                                                                                                                                                                             agtttgaaaaCA‑GAAATCCGCTTCCCACGG‑AA‑GCAACCATTAAAATCGGAAAGAAAAA‑CATCAAC‑ATATGGCTTTTTGGGGTGGCGCCCCGGT‑TAG‑CGTTTCACT‑TCCTGC‑GAAAG‑GGCCCTCCCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAAt                                     <  1:322184/243‑1 (MQ=255)                                                                                                                                                                                             aGGCATGAAACA‑GGAATTCGCTTACCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCCGGTATCGCTCCAGG‑CAG‑CGTTTAACT‑TCCTGC‑CTACA‑GGCGCTACCCCGCCTCTCTCCGGACTGTTGAACAACGGTCTATACTTCAACGCTCTTGTTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTAATCGAACCGTAAAATATATTGACCACCATTCGAATc                                    >  2:1330/1‑251 (MQ=255)                                                                                                                                                                                                   gAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCTCCAGG‑TAG‑CGTTTCACT‑TCCTGC‑GTAAA‑GGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGttt                                                                                                             >  2:281933/1‑172 (MQ=255)                                                                                                                                                                                                                cacgctcaccagagaagtaccaatgtAAAGCGGTAATAAAAA‑CATCAAC‑ATATGGTGTGTGCGGGTCCCGCCCCAGG‑TTG‑TTTTTCACT‑CCCTG‑‑GTAAAAGCCCCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCTATCTAAAt                    <  2:436090/226‑1 (MQ=255)                                                                                                                                                                                                                cGCTTCCCCCGG‑AA‑GCACCAATTTAAA‑CCGTAAATAAAAACAACAAC‑TTTTCGCGTTTTCGGGTAGCGC‑CCAGG‑TTGTCTTTTCCCT‑TCCTGC‑GTAAG‑GGCGCTTCCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGt                  <  1:221221/251‑1 (MQ=255)                                                                                                                                                                                                                   tttCCACGG‑AA‑GCAACCATTTAAATCGGAAATAAAAA‑CATCACCCAT‑TTGCTTATTCGGGTACCGCCCCAGG‑TAG‑CGTTCCTCT‑TCCTGC‑GAAAA‑GGCCCAACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGttt                <  1:554600/250‑1 (MQ=255)                                                                                                                                                                                                                    ttCCACGG‑AA‑GAAACCATTAAAATCGGAAATAAAAA‑AATAAAC‑AATTTGCGTTTTGGGGTAGCCCCCCAGG‑GAG‑CTTTTCACT‑CCCGGC‑TAAAA‑GGCGCTACACCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAAGGTTTg               <  2:661519/250‑1 (MQ=255)                                                                                                                                                                                                                    ttCCACGA‑AA‑GAAACCATTAAAATCGTAAAGAAAAC‑CATCAAC‑ATATGGCGTATTCGGTGAGCGCCCCAGG‑TAG‑CGTTTCACT‑TCCGGC‑TTAAA‑GGCGCTCCCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGtt                 <  2:157912/248‑1 (MQ=255)                                                                                                                                                                                                                           g‑AA‑GCAACCATTAAATTCGGTAAGAAAAA‑AAACAAA‑ATGTTGCGTATTGGGGTAGCCCACAAGG‑TGG‑CGTTTCACT‑CCCTAC‑GAAAA‑GGCGCCACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGt       <  1:534453/251‑1 (MQ=255)                                                                                                                                                                                                                            aaa‑GCAACCATTTAAATCGAAAAGAAAAA‑CAACAAC‑ATAGTGCGTATGCGGGTAGCGCTCCAGG‑TAG‑CGTTTCACT‑TCCGGC‑GAAAA‑GGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGt       <  1:688479/250‑1 (MQ=255)                                                                                                                                                                                                                              aatcacacttatAAATCGTAAAGAAAAA‑CACCAAC‑ATATGGCGTTTTCGGGTCGCCCCCCAGG‑TGG‑CTTTCCCCT‑CCCTGC‑GTAAA‑GGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGGTTGTCAAATGtt      <  2:328703/240‑1 (MQ=255)                                                                                                                                                                                                                                  aacatattAAAGCGGTAAGTAAAA‑CATCAAC‑ATATTGTGTATTCGGGTAGCGCTCCGGT‑TTG‑CGTTTCACT‑TCCGTC‑GTAAA‑GGCGCTCCCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAAt  <  1:644235/245‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               GGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCT‑CGAGCTTCTTCAGGCTTGAAACA‑GGAATACGCTTTCCACGG‑AA‑GCAACCATTTAAATCGGTAAGTAAAA‑CATCAAC‑ATATTGCGTATTCGGGTAGCGCTCCAGG‑TAG‑CGTTTCACT‑TCCTGC‑GTAAA‑GGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAAT  >  NC_000913/1360540‑1361011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑