Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,737,137	IS2 (+) +5 bp	coding (37‑41/825 nt)	bax ←	putative glycoside hydrolase Bax

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3187426	NA (NA)	75 (1.080)	64/488	0.2	100%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	3737137 =	0 (0.000)					coding (41/825 nt)	bax	putative glycoside hydrolase Bax
*	?	NC_000913	= 3737141	0 (0.000)	62 (0.890)	55/490	0.3	100%	coding (37/825 nt)	bax	putative glycoside hydrolase Bax
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region

GGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAACA  >  NC_000913/3187178‑3187663                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              ggTGCGGAGTCCGGAGAGTAAAGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGt                                                                                                                                                                                                                                             >  1:493258/1‑251 (MQ=11) ggTGCGGAGGCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCAGGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGTAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTATGGAAATATAGGGGCAAATCCAGt                                                                                                                                                                                                                                             <  2:22459/251‑1 (MQ=2)      ggAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTatca                                                                                                                                                                                                                                        <  2:95700/251‑1 (MQ=32)            cGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTg                                                                                                                                                                                                                                  <  1:497014/251‑1 (MQ=34)              gagaGGAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGCCGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGt                                                                                                                                                                                                                                <  2:192040/251‑1 (MQ=25)               agagTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATAG‑ATTCGGAAAACACAGCTGGGATATCCAGCCCCACGTGCATATCTGCGGCAGCGGTCTTGTTA‑TGAGATAAGGGATAACAGATGTCTGGAAATATAGGGACAAATCCAGtgaaacagtacagg                                                                                                                                                                                                                                >  1:212408/1‑237 (MQ=2)                  gTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTtgtg                                                                                                                                                                                                                            >  1:74957/1‑251 (MQ=38)                    aaCGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCg                                                                                                                                                                                                                          <  2:495874/251‑1 (MQ=38)                       ggAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGGTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTGATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTGGTAA‑TGGGTTAAGTGATAACAGAGGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCa                                                                                                                                                                                                                       <  1:38625/251‑1 (MQ=255)                        gAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTc                                                                                                                                                                                                                        >  1:527701/1‑249 (MQ=40)                          aTAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCt                                                                                                                                                                                                                    >  1:663566/1‑251 (MQ=255)                            aGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCtt                                                                                                                                                                                                                   <  2:474187/250‑1 (MQ=255)                                 ggaggttCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACTTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGTCACGTGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCg                                                                                                                                                                                                             <  1:109199/246‑1 (MQ=255)                                 agagCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGGTATCGCTCGCCACGGGAATATCTGCGGCTGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCCAATCCCGTTATCATCATTGGTTGTGCGTCAGCTTATGCCg                                                                                                                                                                                                             >  2:180017/1‑251 (MQ=255)                                   agCTTCGGGAAAACGATAAAGCGTTACTACATCAGTATCATGCCCAAACCAGACGGTTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGTTTAAGTGATAACAGATGTCTGGAAATATAGTGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTAGGCCGtt                                                                                                                                                                                                           <  1:473262/251‑1 (MQ=255)                                       tCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGaa                                                                                                                                                                                                        <  1:398923/250‑1 (MQ=255)                                        cGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGGAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAAtt                                                                                                                                                                                                      <  1:614811/251‑1 (MQ=255)                                           gAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTcaa                                                                                                                                                                                                   <  1:83163/251‑1 (MQ=255)                                           gAAAACGATAAAGCGGGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCTAGTTATCAGCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTca                                                                                                                                                                                                    <  2:79490/250‑1 (MQ=255)                                                    aaaGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGAtt                                                                                                                                                                                           <  2:60423/250‑1 (MQ=255)                                                     aaGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTa                                                                                                                                                                                         <  2:270328/251‑1 (MQ=255)                                                      agagggACTACATCAGTATCAGGCCCAAACTAGGCGGGTTAACGGCAGCAAAGACCCTTGCAGAAGCGTTCGAGAATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTTTAGCCCGCCACGGGAATATCCGCGGCCGCGGGCTTGTAA‑TGGGTTAAGTGATAACAAGGGTCTGGAAATATAGGGGCAAATACAGGTATAATCAATGGTTGGTCGTCAGATTATACCGTTGAATTCAAAAAAGGTTTa                                                                                                                                                                                         >  2:607349/6‑250 (MQ=2)                                                               gcagcAGTATCATGCCCAAAGCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGGGC‑GCTGGGTTATCGCGCGTCACGGGAATATCTGCGGCAGGTGGCTTGTAA‑TGGGGTAAGTGATAACAGATGTCTGGAAATATAGGGTCAAAGCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGc                                                                                                                                                                                 <  1:344631/245‑1 (MQ=18)                                                               aCATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGTGTCAGCTTATGCCGTTGAATTCGACAAAGAGTTAATCGAAGcc                                                                                                                                                                                <  1:274318/250‑1 (MQ=255)                                                                         cATGCCCAAACCAGCCGGGTTAACGGCAGCAAAGACCCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTg                                                                                                                                                                      <  2:2392/250‑1 (MQ=255)                                                                          aagCCCAAAACAGACGGGTTAACGGCAGGAAAGAACCTGGCAGAGGCGTGCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCGTGTAAGTGGGT‑AAGTGATAACAGATGGCTGGAAAGATAGGGGCAAATCCAGTTATCATCATTGGTTGTGGGTCAGCTTATGCCGTTGAATTCAGCAAAGATTTAATCGAAGCCGAAGAGCGTGaa                                                                                                                                                                    <  2:278134/249‑1 (MQ=18)                                                                             cccAAACCAGACGGGTTAACGGCAGCACATAACCTTGCAGCGGCGTTCGCGAATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGGCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCTTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAAc                                                                                                                                                                 >  2:36008/1‑251 (MQ=18)                                                                                 aaCCAGACGGGTTAACGGCAGCAAAGAAGCTTGCAGAGGCGTTCGAGCATTATAACGAAGGGCATCCGCATATTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAAGAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTGGTGCGTCAGCTTAGGCCGTTGAATTCAACAAGGATTTAATCTAGGCGGAAGATCGTGAAAACGTTa                                                                                                                                                             <  2:74581/251‑1 (MQ=17)                                                                                    cAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGAGGGCTTGTAA‑TGGGTTAAGTGATAACCGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAAcc                                                                                                                                                          >  2:603522/1‑251 (MQ=255)                                                                                           gTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCTAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAATATCGTGAAAACGTTAACCTTTCCCa                                                                                                                                                   <  2:74957/251‑1 (MQ=255)                                                                                             tAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCaa                                                                                                                                                  <  1:260974/250‑1 (MQ=255)                                                                                              aaCGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTTGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATGTAATCGAATCCGAAGATCGTGAAAACGTTAACCTTTCCCAAtt                                                                                                                                                <  1:601882/251‑1 (MQ=255)                                                                                              aaCGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAAtt                                                                                                                                                <  1:679303/251‑1 (MQ=255)                                                                                                    agcaACGAACCTTGCGGAGTCGGTCGTGCAGTAGACCGAATGGCATCCGCAGAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGTGGCAGCGTGATTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGATATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCATCAAAGATTTAATCGAAGCCGAAGCTCGTGAAAACGTTAACCTTTCCCAATTTGacac                                                                                                                                          <  2:172362/251‑4 (MQ=21)                                                                                                     gcaAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGACATATAGGGGAAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACt                                                                                                                                         >  1:355083/1‑251 (MQ=255)                                                                                                           aaCCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGc                                                                                                                                   >  1:404216/1‑251 (MQ=255)                                                                                                           aaCCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGc                                                                                                                                   <  1:619966/251‑1 (MQ=255)                                                                                                             ccTTGCAGAGGCGGTCGAGCATTATCACGAATGGCATCCGCATAGTGA‑GCTGGGTTATCGCTCGCAACGGGAATATCTGCGGCAGCGGGCTCGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATCTAGGGGCAAATCCAGTTATCATCATTGGTTGCGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCACAATTTGAAACTGATGGcc                                                                                                                                  >  2:646429/1‑250 (MQ=25)                                                                                                                tGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATAGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAGCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCGGTTGAATTCAACAAAGATTTAATCGACGCCGAAGAGCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGctaa                                                                                                                                <  2:504857/249‑4 (MQ=255)                                                                                                                  cAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTAc                                                                                                                            <  1:209623/251‑1 (MQ=255)                                                                                                                       gCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTc                                                                                                                       <  1:250913/251‑1 (MQ=255)                                                                                                                        cGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCg                                                                                                                      <  1:603522/251‑1 (MQ=255)                                                                                                                                cattatcccgcaTGGCATCCGCATAGAGC‑GCTGGGATATCGCTCGCCACGGAAATATCTGCGGCAGCGGGATTGTAA‑TGGGTTCAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGACGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAACCGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAatat                                                                                                              >  2:480795/12‑251 (MQ=37)                                                                                                                                cATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAatat                                                                                                              <  2:527701/251‑1 (MQ=255)                                                                                                                                      aaCGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACt                                                                                                         <  2:663566/250‑1 (MQ=255)                                                                                                                                                      aTAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaat                                                                                         >  1:7277/1‑250 (MQ=255)                                                                                                                                                        aGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGAGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTAAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaataat                                                                                      >  1:622866/1‑251 (MQ=255)                                                                                                                                                              gcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTTATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAagag                                                                                 <  2:423648/251‑1 (MQ=255)                                                                                                                                                                 gggTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGa                                                                               <  1:523563/250‑1 (MQ=255)                                                                                                                                                                         gctcgcCACTGTAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTTATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAATAGGACGCCAATcc                                                                      <  1:627146/251‑1 (MQ=255)                                                                                                                                                                               cACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTg                                                                >  2:273297/1‑251 (MQ=255)                                                                                                                                                                                aCGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGa                                                               <  1:575728/251‑1 (MQ=255)                                                                                                                                                                                  gggAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGAcc                                                             <  1:271849/251‑1 (MQ=255)                                                                                                                                                                                  gggAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGAACGCCAATCCACCTTGAcc                                                             >  1:233461/1‑251 (MQ=255)                                                                                                                                                                                            gCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATg                                                      <  1:477802/248‑1 (MQ=255)                                                                                                                                                                                                   cGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAAGGGGTATTAAtt                                            <  1:590111/251‑1 (MQ=255)                                                                                                                                                                                                         tGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAAc                                      >  2:272446/1‑251 (MQ=255)                                                                                                                                                                                                          gTAA‑TGGTTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCATCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAACTTTAAACTGATTGCCAATTACCCGTCGGCAAATATTCACTATGCTCTCGGATTCATAATAAGATGACGCCAATCCACCGTGCCCTCCAATGGGTATTCATTGATAAc                                      <  2:438914/250‑1 (MQ=255)                                                                                                                                                                                                                  gTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGc                              <  2:79770/251‑1 (MQ=255)                                                                                                                                                                                                                    tAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCa                             <  1:332630/250‑1 (MQ=255)                                                                                                                                                                                                                      aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGtt                          <  1:210592/251‑1 (MQ=255)                                                                                                                                                                                                                        tgataaccggaatCTGGAACTATAGGGGCCTATCCAGCTATCTTCATTGGACGTGCGTCAGCTTATTCCGTTGCCTTCAACACAGATTTAATCGAAGCCGAACATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTCATGCCCAGTTACCCGTCGGCAACTATTAACTAAGAAATATGATTAATAATAAGAGGCCGCCAATCCACCTTGACCTCCAACGGGTATTAATTGATAACAAAACTGCAAtttc                        >  2:463988/13‑250 (MQ=255)                                                                                                                                                                                                                             aCAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCgg                                                                                                                     <  2:480946/153‑1 (MQ=255)                                                                                                                                                                                                                             aCAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCgg                                                                                                                     >  1:480946/1‑153 (MQ=255)                                                                                                                                                                                                                              cAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGac                  >  1:165736/1‑251 (MQ=255)                                                                                                                                                                                                                                    tCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACTAAGATTTAATCGAAGCCGACGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTACTTGCTAACCAAACTGCAGTTTGCGTGACAcc               >  1:324747/1‑248 (MQ=255)                                                                                                                                                                                                                                       ggAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCaga            <  1:155252/248‑1 (MQ=255)                                                                                                                                                                                                                                               aGGGGCAAATCCAGTTATCAGCAATGTTTGGGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACTTTAACCTTTCCCAATGTGAAACTGATGGCCAATTACCCGTCGGCAAATATGCACGAAGCACTCTGATTAATAATAAGAGGTCGCCAATCCACTTTGACCTCCAATGGTGATTAAGTGATAGCCAAACTGCAGTTTTCGTGACTGCAGTTCAATTAACa  <  2:464415/250‑1 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              GGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGC‑GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAA‑TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAACA  >  NC_000913/3187178‑3187663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.