Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,796,208	IS3 (+) +3 bp	coding (535‑537/663 nt)	glaR →	DNA‑binding transcriptional repressor GlaR

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2170171 =	NA (NA)	53 (0.760)	48/490	0.4	100%	noncoding (1/1258 nt)	IS3	repeat region
	?	NC_000913	= 2796210	0 (0.000)					coding (537/663 nt)	glaR	DNA‑binding transcriptional repressor GlaR
*	?	NC_000913	= 2171428	NA (NA)	35 (0.500)	33/490	0.9	100%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	2796208 =	0 (0.000)					coding (535/663 nt)	glaR	DNA‑binding transcriptional repressor GlaR

ATCAGCACCGACAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTC  >  NC_000913/2169930‑2170414                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    aTCAGCACCGACAAACATTAAATCGGCATTAATATCCTGTACTGAACGCTCGGCAATGGAACCATGCATCGAGCGCGATTTACGGCGGACCGTGCCCCCACAGACGACCAGAGACATGTCTTTATTTCAGGAAAGGGCAAAAGCTGGCGGGAGCCTGTTGGTACTCCCCGTGATATTTTTGGCGGGCCTTAAATCTTCGGCGATAAGCAATGGC‑GTACTTCCGCTGTCGCTAATAACGGTCTGTGCTTAcc                                                                                                                                                                                                                                            >  1:307409/1‑251 (MQ=255)     gCACCGACAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGGCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGc                                                                                                                                                                                                                                        >  1:160380/1‑251 (MQ=255)         cGACAAACCTTAAATGCGCCTTACTATCCTGTCATGAACCCTCGCCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGACCCCACAGACGACCAGAGTAATGTCTTTCTTTTCGGAAAGGGCAAACGCTGCCGGGAAACTGTTGGTAATCACCGTGCTTTTTTTGGCGGTAATTAATCCTTCCGCGATAACAATTGTC‑GTACTTCCGCATTCGAGAATAACGGTCTGCTCTTAccacgcaat                                                                                                                                                                                                                                     >  2:72204/1‑243 (MQ=255)           aCAAACATTAAATCGTCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTGCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTGCTTCCGCTGTCTAGAATAACGGTCTGATCTTACCCAGCAATAGt                                                                                                                                                                                                                                  <  1:677459/251‑1 (MQ=255)           aCAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTAcc                                                                                                                                                                                                                                            >  1:275195/1‑241 (MQ=255)           aCAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCAGTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTAcc                                                                                                                                                                                                                                            <  2:275195/241‑1 (MQ=255)               aCATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGac                                                                                                                                                                                                                              >  1:152022/1‑251 (MQ=255)                cATTAAATCGGCATTAGTATCCTGTCATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCAGCCCGAGCCACAAAAGAAGAACAAAGAAAGGTATTTATTTGCGGAAAGGGCAAAAGCTGCCGGGAGAATGTTGGTAATCAAAGGGATATTTTTGGAGGGCATTAATACTTAGGAGATAAGCATTGTCCGCA‑TTCCGCTGGAGAGACTAACGGTCTGATCTTACCAAGCAATAGTGGac                                                                                                                                                                                                                              >  2:685457/1‑250 (MQ=255)                 aTTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGacac                                                                                                                                                                                                                            <  2:15488/251‑1 (MQ=255)                      aTCGGCATTAATATCCTGTAATGACCGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAAAGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTTCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATCGTGGCCACGCGGc                                                                                                                                                                                                                       >  2:248097/1‑251 (MQ=255)                        cGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCCCCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTa                                                                                                                                                                                                                     <  2:163363/251‑1 (MQ=255)                           cATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGt                                                                                                                                                                                                                  <  1:104426/251‑1 (MQ=255)                                  atCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTaa                                                                                                                                                                                                            >  1:189025/1‑250 (MQ=255)                                            gAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCtcagtca                                                                                                                                                                                                 <  2:215082/251‑1 (MQ=255)                                               cGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCagag                                                                                                                                                                                              <  1:513713/251‑1 (MQ=255)                                                   cGGCAATGGAACAAAGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGACAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTACCCTGTCGGAAATAAAGGGCTGATCTTACCCCGCAATAGTGGACACGCGGCTAAGTTAGTAAACTCTCAGTCAGAGGTg                                                                                                                                                                                           >  2:143509/1‑250 (MQ=255)                                                       aaTGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGGGACTca                                                                                                                                                                                      <  2:662499/251‑1 (MQ=255)                                                                      cGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGTGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTTCCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAg                                                                                                                                                                        <  1:283335/250‑1 (MQ=255)                                                                        aGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTCTTTTCGGAAAGGGCAACCGATGCCGGGAGACTGTTGGCAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGAAACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTAt                                                                                                                                                                     >  1:674291/1‑251 (MQ=255)                                                                          cgcgTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCACACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGTATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGtctc                                                                                                                                                                    >  1:449628/1‑247 (MQ=255)                                                                                                 accacAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACa                                                                                                                                            <  1:448002/251‑1 (MQ=255)                                                                                                 accacAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAAGCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAGCCAGTAAAAAACCCCGTAAACa                                                                                                                                            <  1:558472/251‑1 (MQ=255)                                                                                                      aGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCAtt                                                                                                                                       >  1:589296/1‑251 (MQ=255)                                                                                                         cgacCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGc                                                                                                                                    >  1:327648/1‑251 (MQ=255)                                                                                                          gacCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGcc                                                                                                                                   >  1:218768/1‑251 (MQ=255)                                                                                                           acCAGAGTAATGTCTTTATTTTCGGAAAGGGCAACCGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCt                                                                                                                                  >  2:76445/1‑251 (MQ=255)                                                                                                           acCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCt                                                                                                                                  <  1:465566/251‑1 (MQ=255)                                                                                                                  tAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTc                                                                                                                           >  2:98869/1‑251 (MQ=255)                                                                                                                     tGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGc                                                                                                                         >  1:130519/1‑250 (MQ=255)                                                                                                                           tATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTAGCAGCCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGaa                                                                                                                   <  1:272548/250‑1 (MQ=255)                                                                                                                              tttCGGAAAGGGCAAACGCTGCCGGGCGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGccc                                                                                                               >  2:275755/1‑251 (MQ=255)                                                                                                                              tttCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTGCGCAGTGAAGcc                                                                                                                <  1:524885/250‑1 (MQ=255)                                                                                                                                   gAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAg                                                                                                          <  1:239185/251‑1 (MQ=255)                                                                                                                                        ggCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCATTCCGCGATAAGCATTGTC‑GTACTTCCGCTGTCGCGAATAACGGTCTGATCTTACCCAGCAATCGTGGACACGCGGCTAAGTGAGAAAACTCTCAGTCCGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGCGAAGCCCTGACGCTTGc                                                                                                     >  2:645898/1‑251 (MQ=21)                                                                                                                                           aaaCGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGGCGAGAATACCGGTCTGATCTTACCCAGCACTAGTGGCCACGCGGCTAAGTGAGTAAACTCTCAGCCAGAGGTAACTCACATGACAAAAACAGTATCAACCAGTAAAAATCCCCGTAAACAGCATTCGCTTGAAATTCGCAGTGAAGCCCTACAGCTTGCTg                                                                                                   >  2:570182/1‑250 (MQ=21)                                                                                                                                              cGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACg                                                                                               <  2:44412/251‑1 (MQ=255)                                                                                                                                               gCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGATAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGc                                                                                              >  2:97171/1‑251 (MQ=255)                                                                                                                                                   ccGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCg                                                                                          <  2:189025/251‑1 (MQ=255)                                                                                                                                                     gggAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTAGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGCGTAAACTCTCAGTCAGAGGTGACTCACATGCCAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCgg                                                                                         >  1:111702/1‑250 (MQ=255)                                                                                                                                                        agaCTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGtt                                                                                     <  1:539641/251‑1 (MQ=255)                                                                                                                                                        agaCTGTTGGTAATCACCGTGATATTTTTGGCAGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGtt                                                                                     >  1:262603/1‑251 (MQ=255)                                                                                                                                                          aCTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTAc                                                                                   <  1:248097/251‑1 (MQ=255)                                                                                                                                                                            gATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCAGCTGTAGAGAATAACGGTCTGCTCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGAAAAAAACCCCGTAAACAGCATTCGACTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGACCGCATCGGTGTTACTGCCGCAGCCCGTGAACt                                                                 >  2:341752/1‑251 (MQ=255)                                                                                                                                                                                         gTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGa                                                     >  1:95857/1‑250 (MQ=255)                                                                                                                                                                                             ttAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATcac                                                >  1:339386/1‑251 (MQ=255)                                                                                                                                                                                              tAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATcac                                                >  1:287985/1‑250 (MQ=255)                                                                                                                                                                                                             aaGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGCACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGgcg                                <  1:121309/251‑1 (MQ=255)                                                                                                                                                                                                              aGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGgcgc                               >  1:590072/1‑251 (MQ=255)                                                                                                                                                                                                                   tGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCATAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGGAAACTGCATTCGCCTGAATTTCGCATTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTa                           <  1:336226/250‑1 (MQ=255)                                                                                                                                                                                                                          aCTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCa                    <  1:9944/251‑1 (MQ=40)                                                                                                                                                                                                                             tCCGCTGTCGAGAATAACGGTCTGAGCTTACCCATCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCGGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGGGTTACTGCCGCAGCCCGGGAACGCAGCCTGGATGAGTCACAACTCTACAACTGGCGCAGTaagcatcaa                   <  2:106104/249‑8 (MQ=14)                                                                                                                                                                                                                              ccGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGATAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCaaaa                 <  1:97171/250‑1 (MQ=38)                                                                                                                                                                                                                               cGCTGGCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGGCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTTAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGGATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATc               <  2:327648/251‑1 (MQ=255)                                                                                                                                                                                                                                   gTCGAGAATAACGGTCTGATCTTACCCAGCAACAGTGGTCACGCGGCTAAGTGAGTAAACGCTCAATCAGAGGAGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTTAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCCCAACTCTACAACTGGCGCAGTAAACAGCTAACTcagca           <  2:567553/251‑1 (MQ=11)                                                                                                                                                                                                                                      gagaATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAACCCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGAc        >  1:53871/1‑251 (MQ=21)                                                                                                                                                                                                                                      gagaATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCTGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGAc        <  2:330781/251‑1 (MQ=21)                                                                                                                                                                                                                                            aaCGGTCTGATCTTACCCAGCAATAGTGTACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGtcttc  <  2:128751/251‑1 (MQ=14)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    ATCAGCACCGACAAACATTAAATCGGCATTAATATCCTGTAATGAACGCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTC‑GTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGCTGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAACTCTACAACTGGCGCAGTAAACAGCAAAATCAGCAGACGTCTTC  >  NC_000913/2169930‑2170414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.