Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,284,0440TA30.9% 67.1 / 10.3 42T287S (ACA→TCA) actPacetate/glycolate:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (21/8);  new base A (2/11);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 8.41e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATA‑TTCCGG‑ATTCGCACCAACCA‑GCATGA‑TCG‑CGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCC‑ATAAA‑CCCGGTGGCGTAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCC  >  NC_000913/4283795‑4284286
                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   
gggggATAGTGGCGAAAGCAACCGCGTAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATA‑TTCCGG‑ATTCGCACCAACCA‑GCATGA‑TCG‑CGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCC‑ATAAA‑CCCGGGGGCGTAGAACACGCTCTTA‑CGTGCTTCG‑CGTGCATCGCTGACTGTa                                                                                                                                                                                                                                                    <  2:300923/248‑1 (MQ=255)
 gAGGCTAGTGGCGAAAGCAAACGCTGAACTAAAACCGAGGAACAGGTTGCCGCCCACTGCATACGCCAGGTGTAAGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATA‑TTCCGG‑ATTCGCACCAACCA‑GCATGA‑TCG‑CGCCGCACCCGATAATAGAGGGCAGCCTATAGAAGTAGCCC‑ATAAC‑CCCCGTGTCGTACAACACGCTCTTA‑CGTGCTTCG‑CGGGCATAGCTGACTGTa                                                                                                                                                                                                                                                    >  2:101851/1‑250 (MQ=255)
                agaaaCCGCTGAAATAAAACCGAGGAACAGGTGCCCGCCCACGGCATTCGCCAGGTGAACGGCCGCCATGGTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATA‑TTCCGG‑ATTCGCACCAACCA‑GCATGA‑TCG‑CGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCC‑ATAAA‑CCCGGTGGCGGAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCTGCTCTGACTGTAAAGAGGCGCAGCAGaa                                                                                                                                                                                                                                    <  2:168526/248‑1 (MQ=255)
                         tGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATA‑TTCCGG‑ATTCGCACCAACCA‑GCATGA‑TCG‑CGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCC‑ATAAA‑CCCGGTGGCGTAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGAATCAGAATGTGCGGc                                                                                                                                                                                                                            >  1:220232/1‑250 (MQ=255)
                          gAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGGTACCACCAATCAGAGGGCCCGCCGCGTCTTTATA‑TTCCGG‑ATTCGCACCAACCA‑GCATGA‑TCG‑CGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCC‑ATAAA‑CCCGGTGGCGTAGAACACGCTCTGA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCTTCAGAATGTGCGGCaa                                                                                                                                                                                                                          <  2:124132/251‑1 (MQ=255)
                                     gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATA‑TTCCGG‑ATTCGCACCAACCA‑GCATGA‑TCG‑CGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCC‑ATAAA‑CCCGGTGGCGTAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTAc                                                                                                                                                                                                               >  2:87126/1‑251 (MQ=255)
                                     gAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATA‑TTCCGG‑ATTCGCACCAACCA‑GCATGA‑TCG‑CGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCC‑ATAAA‑CCCGGTGGCGTAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTAc                                                                                                                                                                                                               >  1:252366/1‑251 (MQ=255)
                                                    gccCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATA‑TTCCGG‑ATTCGCACCAACCA‑GCATGA‑TCG‑CGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCC‑ATAAA‑CCCGGTGGCGTAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTccc                                                                                                                                                                                                 >  1:118454/1‑250 (MQ=255)
                                                                           cGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATA‑TTACGG‑TTTCGCACCAACCA‑GCATGA‑TCG‑CGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCC‑ATAAA‑CCCGGTGGCGTAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTCCCAAACATCAGTCCCAGACCCAGAGAGAGCGCgg                                                                                                                                                                              >  2:198723/1‑246 (MQ=255)
                                                                                 ccgtgttgtTACCACCAACCAGATGGCCCCCCGCGTTTTTATA‑TTCCGG‑ATTCGCACAAACCA‑GCATGA‑GCG‑CGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCC‑ATAAA‑CCCGGTGGCTTAGAACACGCTCTTA‑CTTGCTTCG‑CGGGCATCGCTGACTGAAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCt                                                                                                                                                                   <  1:301475/248‑1 (MQ=255)
                                                                                  cATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATA‑TTCCGG‑ATTCGCACCAACCA‑GCATGA‑TCG‑CGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCC‑ATAAA‑CCCGGTGGCGTAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCtt                                                                                                                                                                  >  1:264948/1‑251 (MQ=255)
                                                                                  atggtgttcaccacaAACCAGAGGGCCCGCCGCGTCTTTATA‑TCCCGG‑ATCGGCCCCAACCA‑GCATGA‑TCG‑CGCCGAAGCCGAAAAAAAAGGTAAGAATATAGAAGTAGCCC‑AAAAA‑CCGGGGGGCGAAGAACCCGCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAGGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCtt                                                                                                                                                                  <  1:47523/242‑1 (MQ=255)
                                                                                   ggttggttaccccaaatcaatgggCCCGCCGCGT‑TGTATAATTCCGG‑ATTCGCCCCAACCA‑TCATGA‑TCG‑CGCGGAGGCGGATAAAAAAGGTAAGAAAACAGAAGGAGCCC‑ATAAT‑CCGGGGGGCGTAGAACTCGCTCTTT‑CGTTGTTCG‑CGGGCATCGCGGACTGTAAAGAAGCGACTCAGAATGTCCGGCAACCCCGCCTTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCttt                                                                                                                                                                 <  1:220185/229‑1 (MQ=255)
                                                                                   aTGTTGTTCCCACCAATCAGATGGCCCGCCGCGTCTTTATA‑TTCCGG‑ATTCGCACCAACCA‑GCATGA‑TCG‑CGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCC‑ATAAA‑CCCGGTGGCGTAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAACCATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCttt                                                                                                                                                                 >  1:200972/1‑251 (MQ=255)
                                                                                                    aaagtgcccggccgcGTTTTAATA‑TTCCGG‑ATTCGCCCCACCCA‑G‑ATGAACCG‑CGCCAAAGCGGATAATAAAGGGCAGAATAGAGAGGGAGCCC‑ATAAA‑CCGGGGGGGGTAGAACGCGCTCTTA‑CGTGCTCCG‑CGGGCATCCCGGATTGAAAAGAAGCGCACCAGAAGGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCTAGAGAGAGCGCGGAGATCGGATCTTGCTCCAGCCCGCCCGGCt                                                                                                                                                <  2:162433/241‑1 (MQ=255)
                                                                                                         ggCCCGCCGCGTCTTTATA‑TTCCGG‑ATTCGCACCAACCA‑GCATGA‑TCG‑AGCGGCCGCCGATAATACAGTGCAGAATCTCGAAGTAGCCA‑ATAGA‑CCCGGTGGCGTAGTACCCGCTCTTA‑CGTGTTTAG‑CGGGCATGGCTGACTGTAAAGAAGCGCATCAGCATGCGCGGCAAGCCCGCCGGACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGCAACTTTCACCAGCCCGCTCCGCTTCatc                                                                                                                                           >  1:201130/1‑250 (MQ=255)
                                                                                                          ccgcgccgctgttttATA‑TTCCGG‑AT‑CCCCCCAACCAAGAATGC‑ACG‑CGCCGAAGCGGAAAAAAAAGGACAAAATATAAAAGTACCCC‑AAAAC‑CCCGGTGGCGAAAACCACGCTCTTG‑CGTGCTTCG‑CGGCCATCGCTGACTGAAAAGAAGCCCATAAGATTTGGCGACAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCatg‑a                                                                                                                                         <  2:29829/239‑1 (MQ=255)
                                                                                                             cgccgcGTCTTTA‑A‑TTCCGGGATCCGCACCAACCA‑GAATGA‑TCG‑CGCGGAACCCGATAATAAAGGAAAGAAAATAGAAGTAGCCC‑ATAAA‑CCCGGGGGCGTAGAACACGCTCTTA‑GGTGCTTCG‑CGGGCATCTCTGACTGAAAAAAAGCGCATAAGAATGTGGGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGt                                                                                                                                      <  2:295618/251‑1 (MQ=255)
                                                                                                                       ttATA‑TTCCTG‑AGCCGCCCCAACCA‑GCATGA‑‑CGTCGCCGAAGCGTATAAAAAAGGCCAGAATATAAAAG‑AGCCCCATACA‑CCCGGTGGCGAAGAACCCCCTCGTC‑CGTG‑TTCGGCGGGCTTCGCGGACTAAAAAGAAGGGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGGCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACTCCtt                                                                                                                             <  2:276567/250‑1 (MQ=255)
                                                                                                                                              acca‑GCATGA‑TCG‑CGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCC‑ATAAA‑CCCGGTGGCGTAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTc                                                                                                       >  1:8993/1‑251 (MQ=255)
                                                                                                                                                  cgCATGA‑GCG‑CGCCGAAGCCGAAAATAAAGGAAAAAATAAAAAAGTAGCCA‑ATAAC‑CCCGGGGGCGAAGAACCCGCTCTTA‑CGGGCTTCG‑CGGGCATCGCTATCTGAAAAAAAGCGCATCAGAATGGGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCATCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACt                                                                                                    <  2:146774/250‑1 (MQ=255)
                                                                                                                                                        a‑TCG‑CGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCC‑CTAAA‑CCCGGTGGCGTAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAAc                                                                                                >  1:194990/1‑249 (MQ=255)
                                                                                                                                                         gaccccgcaaaaGCCGAAAATAAGGGAAAGAATATAGAAGTA‑CCC‑AAAAAGCCCGGTGGGGTAGAACCCGTTTTTC‑CGTGCTGCG‑CGGGCATCGCGGACTGAAAAGAAGCGCATCAGATTGGGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACa                                                                                               <  1:11281/241‑1 (MQ=255)
                                                                                                                                                          tag‑cgcCTAAGCCGATAATAAAGGACAGAATATAGAAGTAGCCC‑ATAAA‑CCCTGTGGCGTAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGCGCGCGGTGATCGCATCTTTCACCAGCCCGCCCGGCCT‑ACGTATGTCGACGCCTTCCGGGTGTACAGTCATCGTTTCACTGAAc                                                                                                >  2:80973/3‑248 (MQ=255)
                                                                                                                                                           cg‑cgCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCC‑ATAAA‑CCCGGTGGCGTAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAAc                                                                                                >  2:56396/1‑247 (MQ=255)
                                                                                                                                                                ccaagcccaaaaaaaaagacaaaaaTAAAGAAGTACCCC‑AAAAC‑CCCGGTGCCGTAAAACCCGCT‑TTATCTTGCCACG‑CGGGCACCGCTGACTGAAAAGAAGCGAATCAAAAGGTGCGGAAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGAttgtt                                                                                        <  2:125528/228‑1 (MQ=255)
                                                                                                                                                                                  gTCAGAATATAGAAGTAGCCC‑AAAAC‑CCCGGTGGCGCAGAACCCGCTCTTA‑CGTGCTCCG‑CGGGCATCGTGGACTGTAAAGAATCGCATCAGAATGTGCGGCGAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACgtg                                                                      <  1:62838/251‑1 (MQ=255)
                                                                                                                                                                                    aaGAATATAGAAGTAGCCC‑ATAAC‑CCCGGGGGCGAAAAACCCGCTCTTC‑CGTGCTCCG‑CGGGCATCGCGGACTGTAAAGAAGCGCATCAGAAGGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGtt                                                                    <  1:234786/250‑1 (MQ=255)
                                                                                                                                                                                     aGAATATAGAAGTAGCCC‑ATAAA‑CCCGGTGGCGTAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCATCGGTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGAGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGttt                                                                   >  1:38499/1‑251 (MQ=255)
                                                                                                                                                                                       aaaaatatagatgcccaaTAAC‑CCCGGTGGCGTAGAACCCTCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCAACAGAAGGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTGCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTtc                                                                  <  1:48668/234‑1 (MQ=255)
                                                                                                                                                                                        atatAGAAGTAGCCC‑ATAAA‑CCCGGTGGCGTAGAACACGCTCTTA‑CGAGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGCCGCGTTtcat                                                                >  2:248232/1‑251 (MQ=255)
                                                                                                                                                                                             gAAGTAGCCC‑ATAAA‑CCCGGTGGCGTAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGCCCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAACGCCGACGTGTTTCATCAcc                                                            >  2:33305/1‑250 (MQ=255)
                                                                                                                                                                                                         aaaa‑CCCGGGTGCGTAAAACACGCTCTTT‑CGTGCTCCG‑CGGGCATCTCTGACTGAAAAAAATCGCATAAGAAGGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTGCATCACCATAAAGGCCATa                                                <  1:68178/250‑1 (MQ=255)
                                                                                                                                                                                                              cccGGTGGCGAAGAACCCCCTCTTA‑CGTGCTTCG‑CGGGCACCGCTGACTGAAAAGAAGCGCATCAGATTGGGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGc                                            <  1:104244/251‑1 (MQ=255)
                                                                                                                                                                                                                     gcgaaGAACACGTTCTTA‑CGTGCTCGG‑CGGGCACCGCTAACTGAAAAGAAGCGCATCAGAATGTGCGGAAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCAcc                                     <  2:264948/247‑1 (MQ=255)
                                                                                                                                                                                                                       gTAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGAGTTTCATCACCATAAAGGCAATAAAGCTGGCACCg                                    >  2:153946/1‑250 (MQ=255)
                                                                                                                                                                                                                                 ctctTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGCCGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGaacagcaac                           >  2:85229/1‑249 (MQ=255)
                                                                                                                                                                                                                                                       aacGCTGACTGAAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGGGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGcc     >  2:208840/3‑250 (MQ=255)
                                                                                                                                                                                                                                                        tCGCTGACTGAAAGGAAGCGCATCAGAATGTACGGCAAGCCCCCCGTACCAAAAATCAGTCCCAGCCCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACCGATTGTTGAAGCTAAAGCCGACGTGTTTCTTCCCCATCAAGGCCATAAAGCTGGCACCGCACAGCCACAGCACGGCTTTGATAATTTGCAc    >  2:301371/1‑251 (MQ=255)
                                                                                                                                                                                                                                                          gCTGACTGTCAAGAAGCGCATAAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGCCCCACAGAGAGCGCGGCGATCCGATCTTCCACAACCCCACCCGGCTTCATG‑GTGTCGGCCCCTTTCGGGCGTACCGCCATCTCTTCACTGAACAGATTGTCGAAGCTAAAGCCGACGTGTTTCCTACCCATAAAGCCCCTAAAGCTCGCACCGAACAGCAACAGCCCGTCtttcgtcatttgccccc  >  2:103011/1‑237 (MQ=255)
                                                                                                                                                                                                                                                          gCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCAccc  >  1:42719/1‑251 (MQ=255)
                                                                                                                                                                                                                                                            tGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCCTAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCAccc  >  1:276731/1‑249 (MQ=255)
                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   
CGAGGATAGTGGCGAAAGCAACCGCTGAAATAAAACCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATA‑TTCCGG‑ATTCGCACCAACCA‑GCATGA‑TCG‑CGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCC‑ATAAA‑CCCGGTGGCGTAGAACACGCTCTTA‑CGTGCTTCG‑CGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATG‑ATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACAGCAACAGCACGGCTTTGATAATTTGCACCC  >  NC_000913/4283795‑4284286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑