Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,444,9250CG52.5% 3.5 / 65.7 41G102A (GGT→GCT) rpsE30S ribosomal subunit protein S5
Reads supporting (aligned to +/- strand):  ref base C (9/10);  new base G (16/5);  total (26/15)
Fisher's exact test for biased strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGCACTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGT  >  NC_000913/3444684‑3445174
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                         
tGCACTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACAACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCCTTGCACCACCGGCGATGATACCGGTAGCTTCGGa                                                                                                                                                                                                                                                    >  1:29563/1‑249 (MQ=255)
      gCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCAGATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACGTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCt                                                                                                                                                                                                                                            <  2:279368/251‑1 (MQ=255)
                 tAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCAAGCGACTTCCAGAACGGCGCGCATTGAACCACCGGCGATGATACAGGTCCCTTCGGACGCCGGCTGCATGAATAcg                                                                                                                                                                                                                                 >  1:152347/1‑251 (MQ=255)
                               tttGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCTACCATTTATGGAGAATTCATATTTTCCAGGCCCTCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGCTGATACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGtgtg                                                                                                                                                                                                                   >  2:83404/1‑251 (MQ=255)
                                      cacgaggctcgtccgatctttCTTCAACGGAGTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGCCTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACTGGTACCGTCGGAAGCCGGCTGCAGGAATACGCGAGAACCCGTGTGAACGCCt                                                                                                                                                                                                            <  2:197142/233‑1 (MQ=255)
                                                  ccAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGgtgt                                                                                                                                                                                                >  1:140439/1‑251 (MQ=255)
                                                     gAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGc                                                                                                                                                                                             >  2:76410/1‑251 (MQ=255)
                                                         ttcttcAACAGATTTACCACGCTTGGCAGCGACCATTTCTGGAGACTTAATCTTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGCACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCAAGAATACGCGAGAACCCGTGTGAACACCt                                                                                                                                                                                                            >  1:197142/1‑232 (MQ=255)
                                                               aaCGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATAGTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGt                                                                                                                                                                                   <  2:222097/251‑1 (MQ=255)
                                                                                tGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGGTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGt                                                                                                                                                                    <  1:205381/249‑1 (MQ=255)
                                                                                      cGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGCTCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACATTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGGGTTGCAGAGTGCCGTTATTCAgcg                                                                                                                                                                          >  2:202243/1‑237 (MQ=255)
                                                                                      cGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAgcg                                                                                                                                                                          <  1:202243/237‑1 (MQ=255)
                                                                                        aCCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATAtt                                                                                                                                                          <  1:309981/251‑1 (MQ=255)
                                                                                                    gaATTCATATTTTCCAGGCCAGCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCttt                                                                                                                                              <  2:76895/251‑1 (MQ=255)
                                                                                                         cATATTTTCCCGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGGGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCAAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTAGCTTCGGAAGCCGGCTGCATGACTACGCGAGAACCCGTGTGAACACCTTGAACAGGGTGTTGCAGAGTGCCGTGATTCAGCGCGACGTTACTCATATTGCGACGGGCTTTTTcc                                                                                                                                          >  2:137509/1‑250 (MQ=255)
                                                                                                                 ccAGGCCATCACTAGTTGCACGAATAACGTTGATCGGGTCGGTGGAACCATACGCTTTAGCCAGCACGCTATGCACCCCAGAGACTTCCAGAACGGCGCGCATTGAACCACCGGCGATGATACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACCGGGAGTTGCCGAGTGCCGTTATTCAGCGCGCCGTTAATCATATTGCGACGGGCTTTTTCCCTCGCTTTc                                                                                                                                 >  2:194732/1‑251 (MQ=255)
                                                                                                                                  gCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAAc                                                                                                                <  2:164124/251‑1 (MQ=255)
                                                                                                                                      gAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTc                                                                                                             >  1:264041/1‑250 (MQ=255)
                                                                                                                                                gATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGCCTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTAc                                                                                                  >  1:161443/1‑251 (MQ=255)
                                                                                                                                                     ggTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTa                                                                                              >  1:100299/1‑250 (MQ=255)
                                                                                                                                                         ggtggAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTaacca                                                                                          >  2:254450/1‑250 (MQ=255)
                                                                                                                                                                       cTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCg                                                                           >  2:61682/1‑251 (MQ=255)
                                                                                                                                                                        tttAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGt                                                                          >  1:246014/1‑251 (MQ=255)
                                                                                                                                                                            gCCAGCACGTTATCGACCCCAACGACCTCCCGAACGGCGCGCACTGCCCCAACGGCGATAACACCGGTAACTTCCGACGCCGGCTGCATGAATACACGAGAACCCGTGTGAACACCTTTAACCGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGCACTTCACGCGCTTTACCGTAACCAAAACCAACCCGACCGTTAcc                                                                      >  2:44233/1‑251 (MQ=255)
                                                                                                                                                                                         gAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACa                                                         <  1:283781/251‑1 (MQ=255)
                                                                                                                                                                                            ccccAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTc                                                      <  2:140439/251‑1 (MQ=255)
                                                                                                                                                                                               cAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCaga                                                   <  1:109923/251‑1 (MQ=255)
                                                                                                                                                                                                       tCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGa                                            >  1:140390/1‑250 (MQ=255)
                                                                                                                                                                                                           gAACGGCGCGCATTGCACCACCGGCGATGATACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAggcg                                       >  1:17407/1‑249 (MQ=255)
                                                                                                                                                                                                                gcgcgcCTTGCACCACCGGCGATGATACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGCGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACCATCAGAGCTGTTACGGAGaaaa                                   >  2:76166/1‑250 (MQ=255)
                                                                                                                                                                                                                      aTTGCACCACCGGCGATGATACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACgccc                            >  2:287655/1‑248 (MQ=255)
                                                                                                                                                                                                                        tGCACCACCGGCGATGATACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCAcgcg                                                                                                        <  1:258100/173‑1 (MQ=255)
                                                                                                                                                                                                                        tGCACCACCGGCGATGATACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCAcgcg                                                                                                        >  2:258100/1‑173 (MQ=255)
                                                                                                                                                                                                                        tGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGaccac                          >  1:10062/1‑251 (MQ=255)
                                                                                                                                                                                                                                 ggCGATGAGACCGGTACCGTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACATCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATGGCGACGGGCTTGTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGATTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGGCAGAGCTGTGAAGGAGAAAATACGACCTCCTTTAACgg                 <  2:222375/251‑1 (MQ=255)
                                                                                                                                                                                                                                    gatgatACCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGttt              >  1:242392/1‑251 (MQ=255)
                                                                                                                                                                                                                                      tgatACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGtttt             >  2:232933/1‑250 (MQ=255)
                                                                                                                                                                                                                                          aCCGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGAt         >  2:91111/1‑250 (MQ=255)
                                                                                                                                                                                                                                          aCCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGGTTTAGATa        <  1:76410/251‑1 (MQ=255)
                                                                                                                                                                                                                                           ccGGTAGCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATAc       >  1:206714/1‑251 (MQ=255)
                                                                                                                                                                                                                                                aCCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGt  <  1:254450/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                         
TGCACTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGT  >  NC_000913/3444684‑3445174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑