Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,720,634	G→A	intergenic (‑34/‑46)	hokA ← / → insJ	small toxic polypeptide/insertion element IS150 protein InsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,720,634	0	G	A	100.0%	99.9 / NA	29	intergenic (‑34/‑46)	hokA/insJ	small toxic polypeptide/insertion element IS150 protein InsA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/16);  total (13/16)

ATGAGAATGCGCAACGCCGTAAGTAAGGCGGGAATAATTTCCCGCCGAAGACTCTTACTCTTTCAATTTGCAGGCTAAAAACGCCGCCAGCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTT  >  NC_000913/3720394‑3720862                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                     aTGAAAATGCGCAACGCCGTAAGTAAGGCGGGAATAATTTCCCGCCGAAGGCTCTTACTCTTTCAATTTGCAGGCTAAAAACGCCGCCAGCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCAccc                                                                                                                                                                                                                            <  2:142153/251‑1 (MQ=255)    ggaATGCCCACCGCCGTAAGTAAGGCGGGAATAATTTCCCGCCGAAGACTCTTACTCTTTCAATTTGCAGGCTAAAAACGCCGCCAGCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCAtt                                                                                                                                                                                                                         <  2:131115/250‑1 (MQ=255)                         aagGCGGGAATAATTTCCCGCCGAAGACTCTTACTCTTTCAATTTGCAGGCTAAAAACGCCGCCAGCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGa                                                                                                                                                                                                     >  1:45512/1‑250 (MQ=255)                          agGCGGGAATAATTTCCCGCCGAAGACTCTTACTCTTTCCATTTGCAGGCTAAAAACGCCGCCAGCTCCTAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACACATTTTGTTGGACGATGAAATGGAAt                                                                                                                                                                                                   >  2:145733/1‑251 (MQ=255)                           ggctgaaataatTTCCCTCCGAAGACTCTTACTCTTTCAATTTTCAGGCTAAAAACGCCGCCATCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCTGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATa                                                                                                                                                                                                  <  1:135644/245‑1 (MQ=255)                                                       ttactcttTCAATTTGCAGGCTAAAAACGCCGCCAGCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAcc                                                                                                                                                                      >  1:317782/1‑251 (MQ=255)                                                            cttTCAATTTGCAGGCTAAAAACGCCTCCAGCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAGGTCAAAGCCAAAATACCCtt                                                                                                                                                                   <  1:86857/249‑1 (MQ=255)                                                                             aaaaaCGCCGCCAGCTCATAACGCACCTGTTGAAGATGCAATTCACGCAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCTTGAAACAAATCACTATTAAAGAAAGTAAGCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGACTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTGTGAAAAGTGCCTTGAAGt                                                                                                                                                <  1:81863/251‑1 (MQ=255)                                                                                 aCGCCGCCAGCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTTAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAAGCACTATTAAAGAAAGTAATCTATATTTCTGCTGCATTCCCAGCCGCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTg                                                                                                                                            <  2:107212/251‑1 (MQ=255)                                                                                                            tAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCATCGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTCCACACCTGATGATgg                                                                                                                  <  2:349205/250‑1 (MQ=255)                                                                                                                  gCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATACACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTAAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAgg                                                                                                           <  1:237485/251‑1 (MQ=255)                                                                                                                          cacaGTGAGTCTATTCTCATACAGGTGAAAAATAAAATCGTGAACCAAATCACTATTAAAGCAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTAATTCACACCTGATGATGGTTACAGGATCTtctc                                                                                                   >  2:250635/1‑251 (MQ=255)                                                                                                                               tGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCAc                                                                                              >  1:227493/1‑251 (MQ=255)                                                                                                                                gAATCTCTTATTATCGCGGTGAAACATGCAAGCGAGACCCAACTAACTATTACAGAAAGTAATGTATCTTTCTGAGGCCTTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCATAATACCCTTTTGAAAAGCGTCTTGAAGTCGTGCATCACTACTTCACAACTGATGATGGTTACAGGATCATATCGGccct                                                                                             >  2:42406/1‑248 (MQ=255)                                                                                                                                 aaTCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGt                                                                                            >  1:58692/1‑251 (MQ=255)                                                                                                                                                    gAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAAcc                                                                          <  1:316562/250‑1 (MQ=255)                                                                                                                                                     aaaaaTAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCa                                                                        <  2:333526/251‑1 (MQ=255)                                                                                                                                                         aTAAAAGCGTGACACACATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAGTACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCAAGTTTTGGTGTCCCCCGACCCCAGGTc                                                                    >  2:95017/1‑251 (MQ=255)                                                                                                                                                                   gAAACAAATCACTATTAATGAAAGTAATCTAGATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTTCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAATTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTTGTTTCCCCCGAACCCAGGTCAGGACaggg                                                           <  2:384845/250‑4 (MQ=255)                                                                                                                                                                         aaTCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTcac                                                                                                                              <  2:202411/177‑1 (MQ=255)                                                                                                                                                                         aaTCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTcac                                                                                                                              >  1:202411/1‑177 (MQ=255)                                                                                                                                                                                aTTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTAt                                               <  1:212376/249‑1 (MQ=255)                                                                                                                                                                                     agaaagaaATATATATTTCTTCGGCATTCCCAGCCTCCTGTGTTGATTGCCAACGAGTATAGACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCTCCTTGAAGTCGTGAATCACTACGTCTCAACTGATGATGGTTACAGTATCATCTCGGCGCGTTTTGGGGTCCCCCGAACCAATGACAGGACATGGGTTGCCCTCTATGAAAAAc                                        <  1:300580/251‑1 (MQ=255)                                                                                                                                                                                        aaggaaTCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTTCTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCACCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACagg                                     <  2:2179/247‑3 (MQ=255)                                                                                                                                                                                           tAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGccc                                                    <  1:6356/233‑1 (MQ=255)                                                                                                                                                                                           tAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGccc                                                    >  2:6356/1‑233 (MQ=255)                                                                                                                                                                                                   atTTCTGCGGCATTCCCAGCCTCCTGTGCTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGATGCCCTATATGAAAAACATGGAGAAAAAgg                           >  2:160065/1‑250 (MQ=255)                                                                                                                                                                                                                     gCCTCCTGTGTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTaa         >  2:92943/1‑250 (MQ=255)                                                                                                                                                                                                                           tgtgTTGATTTCCAACGAGTATATACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGCAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGCGAAAAAGGTTTAATTCCCAAACCTAAAGGCGtt  >  2:321905/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                     ATGAGAATGCGCAACGCCGTAAGTAAGGCGGGAATAATTTCCCGCCGAAGACTCTTACTCTTTCAATTTGCAGGCTAAAAACGCCGCCAGCTCATAACTCTCCTGTTTAATATGCAATTCACACAGTGAATCTCTTATCATCCAGGTGAAAAATAAAAGCGTGAAACAAATCACTATTAAAGAAAGTAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGATCATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTTTAATTCCCAAACCTAAAGGCGTT  >  NC_000913/3720394‑3720862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑