Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,096	C→T	D204N (GAC→AAC)	dppA ←	dipeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,096	0	C	T	100.0%	186.1 / NA	54	D204N (GAC→AAC)	dppA	dipeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/20);  total (34/20)

AGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCATTCTTCTGCAATTTCGCGTAACGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGTTTTTCTGA  >  NC_000913/3706855‑3707334                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               aGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGAGTTCGGGTACGGCATCACCTGGCATTCATTCTTCTGCAATTTCGCGTAACGCACGGAAGCGTCAAGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTc                                                                                                                                                                                                                                       >  1:361811/1‑251 (MQ=255)    ttGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCATTCTTCTGCAATTTCGCGTAACGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCgg                                                                                                                                                                                                                                    >  2:114907/1‑251 (MQ=255)         ggATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCATTCTTCTGCAATTTCGCGTAACGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTAc                                                                                                                                                                                                                               <  1:98937/251‑1 (MQ=255)                cccTGCTTCATGCGATCGAGATCTGCCGGGTTCGGGTACGGCATCACCGGGCATTCATTCGTCTGCAATGTCGCGTAACTCACGGAAGCGTCAGGGGTAATAGTGAAATCCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGAGACGGGAATCTTTTTGATTCTGCTGTAACTGGAACGGACTGGTTCCGATGGGGTTGAGGTTCAGTTTGTCCGGTGTACCGGCttg                                                                                                                                                                                                                        <  2:166302/250‑2 (MQ=255)                 ccTGCTTCATGCGCGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCATTCTTCTGCAATTTCGCGTAACGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGGTTGGGTTGAGGTTCAGTTTTTCCGGGGTAACGGCTTtc                                                                                                                                                                                                                       >  2:271443/1‑251 (MQ=255)                              agcgaTATCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCATTCTTCTGCAATTTCGCGTAACGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGCTACGGGAATCTTTTTGATACTGCTGTCACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTCCCCGCTTTCATCATCGCATc                                                                                                                                                                                                            >  2:156132/1‑249 (MQ=255)                                    atCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCATTCTTCTGCAATTTCGCGTAACGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATAtt                                                                                                                                                                                                    >  1:124102/1‑251 (MQ=255)                                        gCCGGGTTCGGGTACGGCATCACCTGGCATTCATTCTTCTGCAATTTCGCGTAACGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATAtt                                                                                                                                                                                                    <  1:152742/247‑1 (MQ=255)                                        gCCGGGTTCAGATACGGCATCACCTCGCATTCATTCTTCTGCAATTTCGCGCAACGCACGGAAGCGTCCGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGATTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATCCGGGCATCTTTTTGATCCTGCTGTAACTGGACCGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCCGCATATTCttt                                                                                                                                                                                                >  2:33926/1‑251 (MQ=255)                                             gTTCGGGTACGGCATCACCTGGCATTCATTCTTCTGCAATTTCGCGTAACGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACa                                                                                                                                                                                           <  1:11146/251‑1 (MQ=255)                                                  ggTACGGCATCAACTGGCCTTCATTGTTCTGCAATTTCGCGTAACGACCGGACGCGTCCGGGGTAATAGAGAACACCTGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGCATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGCCAGAAt                                                                                                                                                                                       >  2:65337/1‑250 (MQ=255)                                                    tACGGCATCACCTGGCATTCATTCTTCTGCAATTTCGCGTAACGCACGGACGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATagg                                                                                                                                                                                    >  1:43876/1‑250 (MQ=255)                                                              ccTGGCATTCATTCTTCTGCAATTTCGCGTAACGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTcc                                                                                                                                                                          >  1:385905/1‑251 (MQ=255)                                                                     ttcattcTTCTGCAATTTCGCGTAACGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGcc                                                                                                                                                                    >  1:140341/1‑250 (MQ=255)                                                                          tcttctGCAATTTCGCGTAACGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTc                                                                                                                                                               <  1:95104/250‑1 (MQ=255)                                                                           cttctGCAATTTCGCGTAACGCACGGAAGCGTCAGGGGTAATAGAGTAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTc                                                                                                                                                               >  2:198200/1‑249 (MQ=255)                                                                             tctGCAATTTCGCGTAACGCACGGAAGCTGCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATTCGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGGTGAGGTTCAGTTTTTCCAGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATGGCCAGGTCa                                                                                                                                                              <  2:180253/248‑1 (MQ=255)                                                                                gCAATTTCGCGTAACGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGCGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGCCAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAgg                                                                                                                                                        >  1:19483/1‑251 (MQ=255)                                                                                  aaTTTCGCGTAACGCACGGAAGCGTCAGGGGGAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGa                                                                                                                                                       >  2:16126/1‑250 (MQ=255)                                                                                   aTTTCGCGTAACGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACAGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGCCAGCGAGGAAc                                                                                                                                                     >  1:356765/1‑251 (MQ=255)                                                                                     ttCGCGGAACGCACGTAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCAGCATCGCATCAGCATATTCTGTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACg                                                                                                                                                    <  2:397504/250‑1 (MQ=255)                                                                                              cGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCgg                                                                                                                                          >  2:147593/1‑251 (MQ=255)                                                                                                  cGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGa                                                                                                                                      <  2:45763/251‑1 (MQ=255)                                                                                                            aGGGGCAATCGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGAATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGcaca                                                                                                                             >  2:346022/1‑250 (MQ=255)                                                                                                                  aaTAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGa                                                                                                                       <  1:413952/250‑1 (MQ=255)                                                                                                                    tAGAGAAAACCAGCGAATCGATATGCGGTTTGGTGCCCCAGTAGCCATCAAACGATTTCAAGCGGCTACGGGACTCTTTTTGATACTGCTGTAACTGGCACGGACCGGTTCCGATTGGGTTGAGGGTCAGTTTTTCCGGTGTACCGGCTTTCATCCTCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCCCACACTGAACg                                                                                                                    >  2:273665/1‑251 (MQ=255)                                                                                                                      gagaAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCGTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGgt                                                                                                                  <  1:291161/251‑1 (MQ=255)                                                                                                                         aaaaCCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGtgtt                                                                                                               >  1:91048/1‑251 (MQ=255)                                                                                                                          aaaCCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGtgttg                                                                                                              >  1:255827/1‑251 (MQ=255)                                                                                                                               aGCGTATCGATCTGCGGTTTGGTTCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCGTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAg                                                                                                                                                         <  1:67475/203‑1 (MQ=255)                                                                                                                               aGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACGGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTgtcgtc                                                                                                         >  2:197499/1‑251 (MQ=255)                                                                                                                               aGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCCGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAg                                                                                                                                                         >  2:67475/1‑203 (MQ=255)                                                                                                                                gCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTTTAACTGGAACGGACTGGTTCCGATTGGGTTGAGGTTCTGTTGTGCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCGTTTGACAGAATAGAGGTGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTcc                                                                                                        <  1:187168/251‑1 (MQ=255)                                                                                                                                       gATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATTCGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCtttt                                                                                                 >  1:177984/1‑251 (MQ=255)                                                                                                                                                tttGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCAATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTttcacttcac                                                                                        >  2:23576/1‑251 (MQ=255)                                                                                                                                                  tGGTGCCCCAGTAGCCATCAATCGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCAGCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTtcacttcact                                                                                       <  2:184800/250‑1 (MQ=255)                                                                                                                                                   ggTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTg                                                                                      >  2:323922/1‑250 (MQ=255)                                                                                                                                                          cAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGctc                                                                              <  2:353160/251‑1 (MQ=255)                                                                                                                                                               gccctCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGGCCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCa                                                                         <  2:373337/247‑1 (MQ=255)                                                                                                                                                                        cGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGc                                                                >  1:226947/1‑251 (MQ=255)                                                                                                                                                                             tGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTc                                                            <  1:322868/250‑1 (MQ=255)                                                                                                                                                                               tAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGATCCGATTGGGTTGAGTTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTACCAGGTCAGCGCGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGa                                                          >  2:339512/1‑250 (MQ=255)                                                                                                                                                                                aGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGCCAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGa                                                          >  1:331092/1‑249 (MQ=255)                                                                                                                                                                                  cGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGt                                                       >  1:202479/1‑250 (MQ=255)                                                                                                                                                                                      tACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGATCCGGTTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGCGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTct                                                  >  1:9251/1‑250 (MQ=255)                                                                                                                                                                                           gAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCt                                             >  1:9794/1‑251 (MQ=255)                                                                                                                                                                                                   ttGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTgccgcc                                       >  1:144039/1‑249 (MQ=255)                                                                                                                                                                                                      aTACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGaaa                                  <  1:114907/251‑1 (MQ=255)                                                                                                                                                                                                          tgctgTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCCGAACCttt                              >  2:135737/1‑251 (MQ=255)                                                                                                                                                                                                            ctgTAACAGGACAGGACCGGTGGCGTTTGGGTTGCGGTTCAGTTTTTCAGGTGTACCGGCGTTCATCATCGCATCAGCATATTCTTTTGACAGACTAGAGGTGAAGTCCATTCCCAGGTCAGTGAGGACCGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGGGGTGTCGTCCACCTTTTTCTCTTCACTGATCAGCTCTGGCAAGCCAATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTAt                            >  2:365257/1‑251 (MQ=255)                                                                                                                                                                                                                     gAACGGACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGt                   <  1:16020/251‑1 (MQ=255)                                                                                                                                                                                                                          gACCGGTTCCGATTGGGTTGAGGTTCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTgc              <  1:147593/251‑1 (MQ=255)                                                                                                                                                                                                                                 tCCGATTGGGTTGCGGTTCAGTTTTTCCGATGTACCGGCTTTCATCATCGCATCCGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCACGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGtttt       >  2:394984/1‑251 (MQ=255)                                                                                                                                                                                                                                       ggggTTGATGTTCAGTTTTTCCGGTGTAACGGCTTTCATCATCGCATCAGCATATTCTATGGACAGAATAGATGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGGCAGCACAAACTGAACGGTGTTGTCGGCCCCCTTTTTCTCTTCACTGATCAGCTCTGGAAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGATTTTCTGa  <  2:323367/249‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               AGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCATTCTTCTGCAATTTCGCGTAACGCACGGAAGCGTCAGGGGTAATAGAGAAAACCAGCGTATCGATCTGCGGTTTGGTGCCCCAGTAGCCATCAAACGCTTTGTAGCGGATACGGGAATCTTTTTGATACTGCTGTAACTGGAACGGACCGGTTCCGATTGGGTTGAGGTCCAGTTTTTCCGGTGTACCGGCTTTCATCATCGCATCAGCATATTCTTTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGTTTTTCTGA  >  NC_000913/3706855‑3707334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑