Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,591,960 C→T P122P (CCG→CCA)  ugpB ← sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,591,9600CT97.6% 128.4 / NA 42P122P (CCG→CCA) ugpBsn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (18/22);  minor base G (0/1);  total (18/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTCGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCGGCGCGTTGCCGGTACGAAATGCGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAG  >  NC_000913/3591715‑3592196
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            
cAGGCGCTCAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCCGTTTCGCGGCATAGTCCCCCAGATCCTGCCAGGTTTTAGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGCGACCGGGGGTCGCGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGATCCC‑GTTGGcac                                                                                                                                                                                                                                         >  2:143823/1‑251 (MQ=255)
              ttCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGTCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGTGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCa                                                                                                                                                                                                                            <  2:15512/250‑1 (MQ=255)
                     ttgATCCAGCCCTGCCAGCCGCTTGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGTGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACt                                                                                                                                                                                                                     <  1:354865/248‑1 (MQ=255)
                          ccAGCCCTGCCATGCGCTGGCGTACCCGCACTTCATGCCGGAGGCTTTCATTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTGTCGGCGGCTGTTCCGGGTCTAATCCTGCTGTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATcc                                                                                                                                                                                                               <  1:19046/251‑1 (MQ=255)
                                  gCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCATGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACCGCGACTCATCGAACTGAATCCCTGCctct                                                                                                                                                                                                       >  1:18901/1‑251 (MQ=255)
                                     aGCCGCTCGCGTAGCCGCACTTCAAGGCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGGTCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAAGAGAGACCGGGGGTCGAGCTGTTGAATGGCTGGGACAGTAAGTGGCCCGTTTCGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTTAATCCCTGCATCTTTa                                                                                                                                                                                                    <  2:325477/251‑1 (MQ=255)
                                            ggCGTAGCCGCCCTTCAGGCCGGAGGTTTTCAGTGTCGCCGCAGAGTCCGCCAGATCCTGCCAGGTTTTCTGCGGCTGTGCCTGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATATAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTTAAAGG‑TTTGGCACAAACTGCGACTCATCGAACTGCAGCCCTGCCTTTTTAAACACg                                                                                                                                                                                              <  2:51364/250‑1 (MQ=255)
                                                 aGCCGCACTTCATGCCGGCGGCTTTCAGTTTCGCGACATAGCCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATac                                                                                                                                                                                        >  1:121084/1‑251 (MQ=255)
                                                 aGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGACAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGCAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATac                                                                                                                                                                                        >  1:178564/1‑251 (MQ=255)
                                                     gCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTCAACACGTCATACACCg                                                                                                                                                                                    >  1:340341/1‑251 (MQ=255)
                                                          tCATGCCGGAGGCTTTCGGGTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCAt                                                                                                                                                                                          <  2:232890/240‑1 (MQ=255)
                                                          tCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCAt                                                                                                                                                                                          >  1:232890/1‑240 (MQ=255)
                                                              gCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATTCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGTGAGAGTAATTGGCCCGTTTTGCTGTCGTAGTAGTAACCTGAAACC‑GTTGTCACAAACTGCGACTCATCAAACTGAAGCCCTGCCTGTTGAAACACGTCATACACCGGTGTAATgg                                                                                                                                                                           <  2:276776/251‑1 (MQ=255)
                                                                        ttCAGTTTCGAGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTCGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGc                                                                                                                                                                 >  1:365317/1‑251 (MQ=255)
                                                                              ttCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCcatca                                                                                                                                                            <  2:151031/250‑1 (MQ=255)
                                                                               tCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTTAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCcatcat                                                                                                                                                           <  2:19671/250‑1 (MQ=255)
                                                                                    gCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGc                                                                                                                                                     <  1:335519/251‑1 (MQ=255)
                                                                                     cATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCg                                                                                                                                                    >  2:307439/1‑251 (MQ=255)
                                                                                      aTAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTACGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACCCCGGTTTAATGGATTTCGACGCCATAATGGTGGCgg                                                                                                                                                   >  1:253876/1‑251 (MQ=255)
                                                                                                aGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAGTGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTc                                                                                                                                         >  1:44766/1‑251 (MQ=255)
                                                                                                  aTCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGCGGCGGTGCCAACTTctt                                                                                                                                       >  1:100799/1‑249 (MQ=255)
                                                                                                    ccTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATaa                                                                                                                                     <  1:302804/251‑1 (MQ=255)
                                                                                                                            ccGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCGGCGCGTTg                                                                                                             >  1:195464/1‑251 (MQ=255)
                                                                                                                                    aaTCCTTCTGTCTTGAAGGCGTTTGTGTTGGAATAGAGAACGGGGGGCGATCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAACCTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCTGCGCGTTGCCGGt                                                                                                        <  2:148665/248‑1 (MQ=255)
                                                                                                                                    aaTCCTGTTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTTTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCGGCGCGTTGCCGGt                                                                                                        <  2:363810/248‑1 (MQ=255)
                                                                                                                                          gCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCGGCGCGTTGCCGGTACGAAAt                                                                                                 <  1:73632/249‑1 (MQ=255)
                                                                                                                                            tttCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTACCCTGAAACC‑GTTAGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTCATGGCTTTCGACGCCATCATGGTGGCGGTGCCCACTTCATAAACCTGCAAAATAGCCGGCGCGTTGCCGGTAAGAAATGCgg                                                                                             >  1:393363/1‑251 (MQ=255)
                                                                                                                                             ttCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCGGCGCGTTGCCGGGACGAAACGCGGc                                                                                            >  2:222400/1‑251 (MQ=255)
                                                                                                                                              tCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCGGCGCGTTGCCGGTACGAAATGCGGc                                                                                            <  1:99731/250‑1 (MQ=255)
                                                                                                                                                 tgacggCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAATCCTGCAAAATAGCCGGCGCGTTGCCGGTACGAAATGCGGCAAt                                                                                         <  2:141769/246‑1 (MQ=255)
                                                                                                                                                            ttgttgTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCGGCGCGTTGCCGGTACGAAATGCGGCAATCCCCGCGCtt                                                                               <  1:172667/249‑1 (MQ=255)
                                                                                                                                                            ttgttgTAATAGAGAACGGGGGGCGAGCTGTTGAATGGCTGGGGGAGTAAGTGGCCCGTTTGGCTGTCGGAGTAGTAACCTGAAACC‑GTGGGCACAAACGGCGACTCATCGAACTGAATCCCTGCCTCTGTAAACACGTCATACACCGGTTTAATGGCTTTCAACGCGATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAAGAGCCGGGGCGGTGCCGGTACGAAATGCGGCAATCCCCgcgcg                                                                                <  2:50243/248‑2 (MQ=255)
                                                                                                                                                                 gtAATAGAGTACGGGTGGCTAGCTGTTGAATGGCTGGTAGAGTAAGTGGCCCGGTTGTCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCGCAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCATTCGACGCCATCATGGTGGCGGTACCAACTTCATAAACCTGCAAAATAGCCGGTGCTTTTCCGGTACTAAATGCGGCAATCCCCGCGCGTAAATTCt                                                                        <  2:244380/251‑1 (MQ=255)
                                                                                                                                                                       gagaACGGGGGTCGAGCTGTTGCATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCACTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCGGCTCGTTGCCGGGACGAAATGCGGCAATCCCCGCGCTTAAATTCTGTTCGt                                                                  >  2:132783/1‑251 (MQ=255)
                                                                                                                                                                                                ggcggggAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCCGT‑GGCACAAACGGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCGGCGCGTTGCCGGTACGAAATGCGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTGGCCTTTATAGGTCGGTACAAt                                         <  2:8734/247‑1 (MQ=255)
                                                                                                                                                                                                      gagaGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCGGCGCGTTGCCGGTACGAAATGCGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTa                                   <  1:222400/251‑1 (MQ=255)
                                                                                                                                                                                                             aGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGGGCCAAATTCATAAACCTGCAAAATAGCCGGCGCGTTGCCGGTACGAAATGGGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCggg                            >  2:163494/1‑251 (MQ=255)
                                                                                                                                                                                                                ggCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCGGCGCGTTCCCGGTACGCAATGCGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCTGTACAATTTTGTAATCCGGGttt                         >  2:198290/1‑251 (MQ=255)
                                                                                                                                                                                                                ggCCCGTTTTGCTGACGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCGGCGCGTTGCCGGTACGAAATGCGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGttt                         >  1:75541/1‑251 (MQ=255)
                                                                                                                                                                                                                             gTCGGAGTAGTAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCGGCGCGTTGCCGGTACGAAATGCGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTaaa              <  1:163494/249‑1 (MQ=255)
                                                                                                                                                                                                                                  agtagtAACCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCGGCGCGTTGCCGGTACGAAATGCGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTgg       <  2:13784/251‑1 (MQ=255)
                                                                                                                                                                                                                                        aaCCTGAAACC‑GTTGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCGGCGCGTTGCCGGTACGAAATGCGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCag  <  2:195464/250‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            
CAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACC‑GTCGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAACCTGCAAAATAGCCGGCGCGTTGCCGGTACGAAATGCGGCAATCCCCGCGCTTAAATTCTGTTCGTAGTTGCCTTTATAGGTCGGTACAATTTTGTAATCCGGGTTTTCGGCGTTAAAACGTTGGGCCAG  >  NC_000913/3591715‑3592196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑