Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,490,113 G→A P23L (CCG→CTG)  argD ← N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,490,1130GA97.7% 135.4 / NA 43P23L (CCG→CTG) argDN‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (14/28);  minor base C (1/0);  total (15/28)
Fisher's exact test for biased strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACTTTCTGGATGGAACTGCACACCTTCCAGATCCCACTG  >  NC_000913/3489868‑3490355
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  
aaaaCACCAAGCGTTCGGCAAACGTTGCCGCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTAGCGGAATAAACTCTGCCAGAGCa                                                                                                                                                                                                                                               <  2:281432/250‑1 (MQ=255)
          gcgTTCGGCAAACGTTGCCGAAATCAGTGTACGCCCAAGACGCATCGCCGGTTCATTGGTTACAACGTTACTGACATGCCACAGAGTTGCGCTCTGGGTTTGTAACGCTTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCTATGCCACTCGCGAAATCGCCATACCCCGTGCCTTGATGACCCCAGATTCGGCTGCCTTGACCTTTTACCGGAATAAACTCTGACAGAACATAAATCGGCa                                                                                                                                                                                                                                     <  2:95492/251‑1 (MQ=255)
                     aCGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTc                                                                                                                                                                                                                           >  1:319750/1‑250 (MQ=255)
                       gTTGCCTCAATCAGTGTACTCTCAAGATGCAGCGCCTGTTCAGTGGTGAAAACGTTACTTATATGCCACAGAGTTTCGCCCGGGGGTTTGTACGCGTGCTCCAGTGCATGATTGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGTCATCCTCCTTGCCTTGCTTAGCCCAAATCCTGCTGCTCTGACCTTTTACCGGGAGAAACTCTTCCAGAGCATAAGTCgttgggttaacatcatc                                                                                                                                                                                                                        <  2:273019/251‑17 (MQ=255)
                        ttGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATc                                                                                                                                                                                                                        <  2:125757/250‑1 (MQ=255)
                                ctCAGTTTAATCCGAAGACGCAGAGCCGGTTCATTGGAGAAAACGTTACTGAAATGCCACAGCGCGTCGCCCTGGGAGTTTAACGCGTTCACCAGCCCACGAGGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGCAATCGAAATACTCCTTGCATTGCTGATCCCAGATTCGGCTGACCAGACATTTTCCCGGAATAAAATCTGCCAGCGCATAAATCGGCAGGATCACTTACTCGAAAGTcac                                                                                                                                                                                                               >  2:266102/2‑249 (MQ=255)
                                   aGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTcgcgcg                                                                                                                                                                                                            >  2:100318/1‑251 (MQ=255)
                                    gTTTACGCCCAAGAACCACCGCCGGTTAATTGGTGAAAACGATACTGATCTGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCAACAGCGCAGGATGGCAATGGCCAAACGCCGTAACTGCAGTGCCACCCGCGAAATCGACATACTCATTGCCTTGCTGATCCCAGATTCGGCAGCCCTGACCTTTATCCGGAATAAACTCTGCCCGAGCATAAATCGGAAGGATCACTTCATCGACAGTcgcgcgg                                                                                                                                                                                                           >  2:272468/1‑250 (MQ=255)
                                                 aCGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCtgttt                                                                                                                                                                                              >  2:14343/1‑251 (MQ=255)
                                                      gcgcCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCa                                                                                                                                                                                         <  1:181279/251‑1 (MQ=255)
                                                       cgcCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGTGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCGTTTACCGGAATAAACGCTGCCAGAGCATAAATCTGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTTTTTATTCaa                                                                                                                                                                                        <  2:275784/251‑1 (MQ=255)
                                                                aTTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATACCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGAAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCAtct                                                                                                                                                                               >  1:280071/1‑251 (MQ=255)
                                                                   ggTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCa                                                                                                                                                                             <  2:103682/250‑1 (MQ=255)
                                                                   ggTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCCTGCTGATACCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATGGGCAGGATCACTTCATCGAAAGTCACGCGTGTAATTCCTGTTTGTTCAATTGCCATCTCAt                                                                                                                                                                            >  1:276117/1‑251 (MQ=255)
                                                                    gTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATg                                                                                                                                                                           <  2:113037/251‑1 (MQ=255)
                                                                            gTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCt                                                                                                                                                                   <  2:110599/251‑1 (MQ=255)
                                                                              tACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGt                                                                                                                                                                 >  1:297733/1‑251 (MQ=255)
                                                                               aCTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCGATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGt                                                                                                                                                                 <  1:274269/250‑1 (MQ=255)
                                                                                      tGCCCCAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCACTGCCACCAGCGAAATCGACATACTCCTTGCCTTGATGATCACAGATTCGGCTGCCCTGACCTTTTACCGAAATAAAATCTGCCAGAGCATAAATCGGAAGGATCACTTCATCGAAAGTCAAGCGTGTAATTGCTGTTTGATCAATTGCCATCTCAAGATACCCCTGTTACGCAt                                                                                                                                                          >  1:409058/1‑250 (MQ=255)
                                                                                       gCCACAGAGTTTCGCCCTGTGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATa                                                                                                                                                         <  1:191727/250‑1 (MQ=255)
                                                                                       gCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATa                                                                                                                                                         >  1:363682/1‑250 (MQ=255)
                                                                                       gCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCGTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTGCAATTGCCATCTCATGATCACCCGGTTACGCATa                                                                                                                                                         <  1:133310/250‑1 (MQ=255)
                                                                                                 ttCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCTCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAAtgtg                                                                                                                                               <  1:328743/250‑1 (MQ=255)
                                                                                                 ttCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAAtgtg                                                                                                                                               <  1:257125/250‑1 (MQ=255)
                                                                                                   cGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCGCCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGAGCACCCTGTTACGCATAAACAAATGTGaaa                                                                                                                                            <  1:126181/251‑1 (MQ=255)
                                                                                                         gggTTTTTAACGCGGTCACCAGCGCAGGATGGCAATTGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATtat                                                                                                                                        <  1:319821/249‑1 (MQ=255)
                                                                                                          ggTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACAGACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCAGAAATCGGCAGGATCACGTCATCGAAAGTCGCGCGTGTAATTGCTTTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAAc                                                                                                                                     <  1:192578/251‑1 (MQ=255)
                                                                                                             ttttAACGCGTTCACCAGCGCAGGATTGCAAGGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACGCCGTGCCGTGCTGACCCCAGATGCGTCTGCCCTTACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGTGTTTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACca                                                                                                                                   <  1:272468/250‑1 (MQ=255)
                                                                                                                tAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACaa                                                                                                                                <  1:114528/250‑1 (MQ=255)
                                                                                                                                               cccAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATc                                                                                                 >  2:309800/1‑250 (MQ=255)
                                                                                                                                                                       cccGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAg                                                                        <  2:171435/251‑1 (MQ=255)
                                                                                                                                                                          gcgAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCca                                                                     <  1:100318/251‑1 (MQ=255)
                                                                                                                                                                            gAAATCGACATACTCCTTGCCTTGCTGATCCCAGCTTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATc                                                                                                                                                                        >  1:150452/1‑150 (MQ=255)
                                                                                                                                                                            gAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATGGCTGTGTGTTCAATTGCCATCTCATGATc                                                                                                                                                                        <  2:150452/150‑1 (MQ=255)
                                                                                                                                                                                 cGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTg                                                              <  2:72770/251‑1 (MQ=255)
                                                                                                                                                                                   aCATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGAt                                                            <  2:297733/251‑1 (MQ=255)
                                                                                                                                                                                             tgccttgcTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTc                                                  >  1:170060/1‑251 (MQ=255)
                                                                                                                                                                                                             gATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAACGTCGAGCGTGTACTTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACTTTCt                                  >  1:388031/1‑251 (MQ=255)
                                                                                                                                                                                                             gATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACttgct                                  <  2:319750/251‑4 (MQ=255)
                                                                                                                                                                                                                       cccTGACCTTTTACCGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACTTTCTGGATGGAACt                        <  2:346165/251‑1 (MQ=255)
                                                                                                                                                                                                                                    ccGGAATAAACTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCGTGTTCGCTAAGAATACTTTCTGGATGGAACGGCACACCTTCCAg           <  2:255127/251‑1 (MQ=255)
                                                                                                                                                                                                                                          tAAACTCTGACAGAGCATAACTCGGCAGGCTCACTTCATCGAAAATCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCCCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCCCAAAATATGCATAAAAAATCAATAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACTTTCTGGATGGAACTGCACACCTTCCAGATccc      >  2:248252/1‑250 (MQ=255)
                                                                                                                                                                                                                                             aCTCTGCCAGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACTTTCTGGATGGAACTGCACACCTTCCAGATCCCACtg  <  2:42086/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  
TAAACACCACGCGTTCGGCAAACGTTGCCTCAATCAGTTTACGCCCAAGACGCAGCGCCGGTTCATTGGTGAAAACGTTACTGATATGCCACAGAGTTTCGCCCTGGGTTTTTAACGCGTTCACCAGCGCAGGATGGCAATGGCCCAACGCCGTAACTGCAATGCCACCCGCGAAATCGACATACTCCTTGCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATACTTTCTGGATGGAACTGCACACCTTCCAGATCCCACTG  >  NC_000913/3489868‑3490355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑