Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,377,150 C→A V91F (GTT→TTT)  sspA ← stringent starvation protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,377,1500CA98.5% 222.1 / NA 65V91F (GTT→TTT) sspAstringent starvation protein A
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (24/40);  total (25/40)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

ATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCGGAAAACA  >  NC_000913/3376903‑3377383
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         
aTGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAgg                                                                                                                                                                                                                                        <  1:11587/251‑1 (MQ=255)
          gctgCAGACGACACAGCAGCGTAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTgg                                                                                                                                                                                                                               <  2:267354/247‑1 (MQ=255)
                 aCGCCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGTTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCg                                                                                                                                                                                                                       <  1:418645/251‑1 (MQ=255)
                   gCCACAGCAGCGGAGCCAGATAGCAAGCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCAACAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCggg                                                                                                                                                                                                                     <  2:39746/251‑1 (MQ=255)
                    ccACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCgggg                                                                                                                                                                                                                    >  1:254394/1‑250 (MQ=255)
                           agcGGAGCAAGATAGCAATCGACCAGGCCGAACTCATCGCTCAGGAAGTACGGCTTCTGAACGAAGACCGGCGCAATCGCTAGCAGTTCTTCGCGCATTTGCTTACGTGCGGCATCTGATTCAGAAGCTGAATCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGTATGTACAGCCGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTc                                                                                                                                                                                                             <  1:236668/251‑1 (MQ=255)
                               gAGCAAGATAGCAAGCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCATCAGTTCTTCGCGCAGTTGCTTACTTGCGGCATCTGCTTCAGATGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGGACATATGGCTGTCTCCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGAAGCGGGAAACGCTCATcc                                                                                                                                                                                                         <  2:187915/251‑1 (MQ=255)
                                   aaGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGat                                                                                                                                                                                                     <  1:410888/251‑1 (MQ=255)
                                      aTAGCAATCGACCAGGCTGAACTCAGCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATAtt                                                                                                                                                                                                  <  2:78683/251‑1 (MQ=255)
                                           aaTCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGCAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGGAACCCTCCTCCAAATATTCCAt                                                                                                                                                                                              >  2:333425/1‑250 (MQ=255)
                                           aaTCGACCAGGCTGAACTCATAGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTccct                                                                                                                                                                                              >  1:302746/1‑248 (MQ=255)
                                                aCCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCGTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAatgat                                                                                                                                                                                        <  2:411633/251‑1 (MQ=255)
                                                 ccAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCAGCCAGATATTCCATAatgatg                                                                                                                                                                                       <  1:162565/251‑1 (MQ=255)
                                                        gAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCgaga                                                                                                                                                                                  <  1:278084/249‑1 (MQ=255)
                                                              aTCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCcaca                                                                                                                                                                          <  1:167009/251‑1 (MQ=255)
                                                                    cAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCa                                                                                                                                                                    <  1:293242/251‑1 (MQ=255)
                                                                     aGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGGAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTc                                                                                                                                                                     >  1:39592/1‑249 (MQ=255)
                                                                        aaGTACGGCTTCTGACCGAAGACCGGCGCAAGCGCCAGCAGGTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGcgc                                                                                                                                                                <  2:329604/251‑3 (MQ=255)
                                                                         aGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCa                                                                                                                                                               <  2:410967/251‑1 (MQ=255)
                                                                                 ttCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATccac                                                                                                                                                       >  1:106183/1‑251 (MQ=255)
                                                                                          aaGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCgg                                                                                                                                              <  1:67936/251‑1 (MQ=255)
                                                                                                    cAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACCGGGTCAGCTCACGATCCACCAAGGGCGGAACGctcttt                                                                                                                                    >  2:90831/1‑249 (MQ=255)
                                                                                                          ccAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTc                                                                                                                                 <  2:161881/248‑1 (MQ=255)
                                                                                                           cagcagTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGACCCACCAGGGTCGGGACGCTCTGGTTCgggg                                                                                                                             >  1:100548/1‑250 (MQ=255)
                                                                                                                gTTCTTCGCGCAGTTGCTTACGTGCGGCCTCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCCCATCCCGATATTTCATAATGATGGGAGAATCCCACCGGGGCAGACCCCGATCCCCCAGGGGCgggacactccgcttagggtttggg                                                                                                                        >  2:290362/1‑229 (MQ=255)
                                                                                                                          cAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTTCATAATGATGCGAGATTCCCAAAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGCCaaac                                                                                                                  >  1:166887/1‑245 (MQ=255)
                                                                                                                          cAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGGTTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATc                                                                                                                  >  1:229533/1‑247 (MQ=255)
                                                                                                                                     gTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCCACAGGGTCGGGACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCggt                                                                                                   >  1:83877/1‑250 (MQ=255)
                                                                                                                                       gCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAtt                                                                                                 <  1:297740/251‑1 (MQ=255)
                                                                                                                                       gCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCAGCAGCGTGTACCAGTCTTTTTCGATGCGAGGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGGCAATCAGAGCCTGAGGCGGAtt                                                                                                 <  2:166887/251‑1 (MQ=255)
                                                                                                                                         ggCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTCCAAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAACCGCTCATACAGATATTCCATAATGATGCGAGATTACCACAGGGGCAGCTCACGAACCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATACTGAGGCGGATTGt                                                                                               >  1:258673/1‑251 (MQ=255)
                                                                                                                                          gCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTc                                                                                              <  1:230463/251‑1 (MQ=255)
                                                                                                                                                gCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCtt                                                                                           <  1:306344/248‑1 (MQ=255)
                                                                                                                                                   tCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTcc                                                                                       <  1:197431/249‑1 (MQ=255)
                                                                                                                                                    cAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACg                                                                                    <  1:327256/251‑1 (MQ=255)
                                                                                                                                                     aGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGGACAGACGGCTTTCACCGCGAGCTACCGGGTACAAAGGCATCAGGGGCGGATGGGGGAAAAGCGCATCCAGATATTCCATAAATATGCGAGATTCCCACAGGGTCCGCTCACGATCCACCAGGGTCGGAAAGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGGGGATTGTCCTTTTCCACgg                                                                                   >  2:320944/1‑250 (MQ=255)
                                                                                                                                                       aaGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACgtgt                                                                                 >  1:346236/1‑251 (MQ=255)
                                                                                                                                                       aaGCTGAACCGTTGATGATGGTGTTCATCAGCGTGAACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGCGGCGGATTGTCCTTTTCCACgtgt                                                                                 >  1:116494/1‑251 (MQ=255)
                                                                                                                                                        aGCTGAACCGTTGATGATGGTGTTTATCAGCGTGTACCATTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGTTCAGCTCACGATCCACCAGTGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCGTTGCCACGTGtt                                                                                <  2:202730/251‑1 (MQ=255)
                                                                                                                                                         gCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTc                                                                               <  2:274874/251‑1 (MQ=255)
                                                                                                                                                         gCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGCATGTCCTTTTCCACGTGTTc                                                                               >  1:192930/1‑251 (MQ=255)
                                                                                                                                                            gAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATAGTCCATAATGATGCGAGAGTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTGTAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCg                                                                              <  1:174056/249‑1 (MQ=255)
                                                                                                                                                             aaCCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAtc                                                                           <  2:45817/251‑1 (MQ=255)
                                                                                                                                                                cGTTGATGATGGTGTTCATCAGCGGGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCg                                                                        <  2:149468/251‑1 (MQ=255)
                                                                                                                                                                     atgatgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACt                                                                   <  1:106463/251‑1 (MQ=255)
                                                                                                                                                                       gatgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTa                                                                 <  2:277802/251‑1 (MQ=255)
                                                                                                                                                                        atgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGCTTGTCCTTTTCCACGTGTTCGATCTCGAAACTTac                                                                >  1:406496/1‑251 (MQ=255)
                                                                                                                                                                         tgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGACACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGctc                                                                                                                                       >  1:70898/1‑179 (MQ=255)
                                                                                                                                                                         tgGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGAGGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGctc                                                                                                                                       <  2:70898/179‑1 (MQ=255)
                                                                                                                                                                          ggTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTaca                                                               <  2:39592/250‑1 (MQ=255)
                                                                                                                                                                                      cGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATAAGTGGCGGCTGCGGGAAACGCTCACCCAGATATTCCATAATGATGAGCGATTCCCCCCGGGTCAGCTCACGATCCAACAGGGTCGGAAAGCTCTGATTCGGGGTGAGGGCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTaagcc                                                   >  1:399726/1‑250 (MQ=255)
                                                                                                                                                                                         gtACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATAATCCATAATGATGCGAGATTCCCACCGGGTCAGCTCACGATCAACCAAGGGCGGGACGCTCTGATTCGGGTTGAGGACACTCATATCCTGAGGCGGGTTGTCCTTTTCCACGTGTTCGATCTCGAAAATTCAACCTTTCTcagccagc                                                >  2:330002/1‑250 (MQ=255)
                                                                                                                                                                                                tCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCg                                        >  1:144620/1‑251 (MQ=255)
                                                                                                                                                                                                       cgatgcgatgCCTGTACCGACGGCTTTCACCGCGCGCTCCCGGGTAACAAGGCATCAGAGGCGGATGCGGGCAACGCCCATCCCGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCCGGGGCGGaa                                                                                                                                            >  2:369161/1‑144 (MQ=255)
                                                                                                                                                                                                       cgatgcgatgCATGTACAGACGGCTTTCACCGCTAGCTACCGGGTAAAAAGGCATCAGTGTCGGATGCGTGAAACGCGCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGaa                                                                                                                                            <  1:369161/144‑1 (MQ=255)
                                                                                                                                                                                                              atgcATGTACAGACGGTTTTCACCGCGAGCCACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATAGTCCATAATGATGCTAGATTCCCACAGGGTCAGCTCACGAGCGACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGGCAATCAGATGCTGAGGCTGTTTGGCCTTTTCCATGTGTTCGATCTCGTAACTTTGGCCTTTCTcagctagctcattgctgccctgggtgctat                          <  2:374533/251‑23 (MQ=255)
                                                                                                                                                                                                                gcATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCGTTCTCAGCCAGCACAATGCGGACCTGATGGCtata                         <  2:23484/250‑1 (MQ=255)
                                                                                                                                                                                                                  aTGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCtat                          >  1:201507/1‑247 (MQ=255)
                                                                                                                                                                                                                            ggCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGGCCTGATGGCTATAGATGTCAGTAgg             >  1:408584/1‑250 (MQ=255)
                                                                                                                                                                                                                             gCTTTCACCGCGAGCTACCGGGTAAAAAGGCTTCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGGCAACTCACGATCCACCAGGGTCGGCACGCCCTGATTCGGGTTGAGGTCAATCCGATCCTGAGGCGGGTTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACCCCTTTCTCAGCCAGCACAAAGCGGACCTGATGGCTATAGTTGTCAGTAgggc           >  2:241943/1‑249 (MQ=255)
                                                                                                                                                                                                                             gCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGAc           <  2:39706/251‑1 (MQ=255)
                                                                                                                                                                                                                             gCTTTCACCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTGTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGAc           <  2:302746/251‑1 (MQ=255)
                                                                                                                                                                                                                                   aCCGCGAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCTGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCGGaaa     <  1:22154/251‑1 (MQ=255)
                                                                                                                                                                                                                                     cgcgAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCGGAAAAc   <  2:254394/251‑1 (MQ=255)
                                                                                                                                                                                                                                      gcgAGCTACCGGGTAAAAAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCGGAAAACa  <  2:103711/251‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         
ATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAGCCAGCACAATGCGGACCTGATGGCTATAGATGTCAGTAGGACCGGAAAACA  >  NC_000913/3376903‑3377383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑