Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,349,949	C→T	V171M (GTG→ATG)	elbB ←	low activity glyoxalase ElbB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,349,949	0	C	T	100.0%	194.8 / NA	56	V171M (GTG→ATG)	elbB	low activity glyoxalase ElbB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/28);  total (28/28)

AAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCACATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACCCCCCCGGCACAATCA  >  NC_000913/3349708‑3350194                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      aaaCAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCcgc                                                                                                                                                                                                                                              >  1:345272/1‑251 (MQ=255)                  cGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCa                                                                                                                                                                                                                             <  1:284893/251‑1 (MQ=255)                   gCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATCTGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGACCATCTCTTCCAGCAc                                                                                                                                                                                                                            >  1:202744/1‑251 (MQ=255)                      ccAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTc                                                                                                                                                                                                                         <  1:187235/251‑1 (MQ=255)                             aCGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGt                                                                                                                                                                                                                   >  1:400534/1‑250 (MQ=255)                                   aGGACAACCATTAACCGCAAAAGCAAACGGCGAACGAAACTACACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGAGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTGCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTAGCGat                                                                                                                                                                                                             >  2:271480/1‑250 (MQ=255)                                            aTTAACAGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGtcccc                                                                                                                                                                                                   >  2:64533/1‑247 (MQ=255)                                                  cGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTc                                                                                                                                                                                             >  1:361198/1‑251 (MQ=255)                                                     aaaaGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCaa                                                                                                                                                                                           >  1:66053/1‑250 (MQ=255)                                                       aaGAAAACGGCGAACGAAACTAAACACTGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCTCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGATAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCAGCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGGCAAACg                                                                                                                                                                                        <  2:267694/251‑1 (MQ=255)                                                          aaaaCGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGCTATCATCGCCAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGc                                                                                                                                                                                       >  2:255819/1‑249 (MQ=255)                                                           aaaCGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACCCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGAGGGTCAAACGCAGc                                                                                                                                                                                    <  2:66053/251‑1 (MQ=255)                                                            aaCGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCg                                                                                                                                                                                   <  1:313765/251‑1 (MQ=255)                                                              cGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCggg                                                                                                                                                                                 >  2:265897/1‑251 (MQ=255)                                                                      gaaACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCCTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGaa                                                                                                                                                                         <  1:37431/251‑1 (MQ=255)                                                                         aCTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAAt                                                                                                                                                                      >  2:299525/1‑251 (MQ=255)                                                                             aaCACCGTTAAGCGGCGTTTACCCATTCAGCCAGAACCAGCACGCGGGAAACCAGCGTAGCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTc                                                                                                                                                                  <  2:367227/251‑1 (MQ=255)                                                                              acacCGTTAAGCGGCTTTTACCCATTCAGCCAGAACCAGCCCGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCg                                                                                                                                                                 <  2:94445/251‑1 (MQ=255)                                                                                 ccGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTcagc                                                                                                                                                               >  2:304747/1‑247 (MQ=255)                                                                                      tagctgCTTTTACTCATACAGCCCGAACCAGCCCGCGGGAAACCAGCTTATCAAAGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAACATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGAGATCATCGACCGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAACTTTCGgcagcct                                                                                                                                                          <  2:269136/245‑3 (MQ=255)                                                                                        gCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGc                                                                                                                                                       >  1:373170/1‑251 (MQ=255)                                                                                         cGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGAAATGCTCTGCGCCAGCATATATGCTGGGGTGGTGCCCATCTTATTGTCTTCATCAACCACGATATCATGGACAGGCCACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGcc                                                                                                                                                      >  1:396166/1‑251 (MQ=255)                                                                                                tACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCa                                                                                                                                                <  1:303218/250‑1 (MQ=255)                                                                                                       gcAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGAATCGCCGGGGCAATAcaca                                                                                                                                         <  2:9027/249‑1 (MQ=255)                                                                                                          gCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCTCTTCTGCTGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATaaa                                                                                                                                     <  1:266845/251‑1 (MQ=255)                                                                                                            cAGACCCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATAGCAGTCCCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAAc                                                                                                                                   <  2:164723/251‑1 (MQ=255)                                                                                                              gAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCa                                                                                                                                 <  2:361198/251‑1 (MQ=255)                                                                                                               aaCCAGCACGCGGGACACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCa                                                                                                                                 >  2:207315/1‑250 (MQ=255)                                                                                                               aaCCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCaa                                                                                                                                <  1:228051/251‑1 (MQ=255)                                                                                                                                               cGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGcc                                                                                                <  2:400534/251‑1 (MQ=255)                                                                                                                                                    gccgcTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATTGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAgcgc                                                                                           >  1:334708/1‑251 (MQ=255)                                                                                                                                                    gccgcTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAgcgc                                                                                           >  1:84222/1‑251 (MQ=255)                                                                                                                                                      cgcTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCt                                                                                         <  1:122971/251‑1 (MQ=255)                                                                                                                                                       gcTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCtt                                                                                        >  2:63802/1‑251 (MQ=255)                                                                                                                                                       gcTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCtt                                                                                        <  2:19930/251‑1 (MQ=255)                                                                                                                                                                   ttCTGCGCCAGCCTATCTGCTGGGGTGGTGCCAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCCTATGCTCAGCGCCCATCTCTTCAAGCACTTCTGCGGCATCGATATCAGTACCGATGATCAAACGCAGCGGGAAATCGAAAATTATCGGCAGCCTCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCATGATGCATCGCTTGTGCCAGCGCCTTTAAtttacagtc                                                                            >  2:201603/1‑244 (MQ=255)                                                                                                                                                                        cgcCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGGCAACgg                                                                       <  2:373170/251‑1 (MQ=255)                                                                                                                                                                        cgcCAGCATATATGCTGGGGCGGTGACAATCTTATTGTCTTCATCCACCACGCTATCATCGCCAGGACACGGCATATGCTCCGCGCCCCTCTCTTCCAGCACTTCTGCGGAATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTACAGCCTGCTGCATCGCTTGTGCAAGCGCCTTTAATTCACGGTCAACgg                                                                       >  2:393815/1‑251 (MQ=255)                                                                                                                                                                         gcCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGt                                                                      <  1:299525/251‑1 (MQ=255)                                                                                                                                                                          ccAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTg                                                                     >  1:327797/1‑251 (MQ=255)                                                                                                                                                                            aGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGCTATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGCAATCGAAAATTTTCGgcagc                                                                                                                                                            >  2:33868/1‑162 (MQ=255)                                                                                                                                                                            aGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGgcagc                                                                                                                                                            <  1:33868/162‑1 (MQ=255)                                                                                                                                                                              cATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCAtt                                                                 >  1:23714/1‑251 (MQ=255)                                                                                                                                                                               atatatGCTGGGGTGGGGACAATCTTATTGTCTTCATCAACCACGATATGATCGACAGGACACGGCATGTGAGCCGCGCACATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGAAGGTCAAACGCAGCGGGAAATCGAAAATTCTCGGCAGCATCGCCGTGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCTCTTCTGCCAGCCCCATCAATTCACGGTAAACCGTGCAtgg                                                                <  2:222198/251‑3 (MQ=255)                                                                                                                                                                                        ggggTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCAGCGCCCATCTCTTCCAGCCCTTCTGCGGTATCGATATCAGTACCGATGGTCAAAAGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCa                                                         >  1:189550/1‑249 (MQ=255)                                                                                                                                                                                         gggTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCa                                                         >  1:343286/1‑248 (MQ=255)                                                                                                                                                                                              gtgACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGc                                                 >  1:412051/1‑251 (MQ=255)                                                                                                                                                                                                       ttATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCt                                        >  1:344444/1‑251 (MQ=255)                                                                                                                                                                                                           tGTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTaa                                     >  1:279173/1‑250 (MQ=255)                                                                                                                                                                                                            ggcTTCATCAACCACGATATCAGCGACAGGACACGGCATATGCTCCGCGCCCATCGCTTCCAGCACGTCGGCGGTATCGATAGCAGTACCGATGGTCAAACGCAGCGTGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTGTTCCGGCCTGATGCAGCGCTTGTGCCAGCGCCTTGAATTCACGGTCAACGTTGCATTCGCTACCAAGACTGCCAAAATTGCTTAAAt                                   <  2:259009/249‑1 (MQ=255)                                                                                                                                                                                                            gTCTTCATCAACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTGCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTaaa                                    <  1:232218/250‑1 (MQ=255)                                                                                                                                                                                                                tcatcaACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTGCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATGCGCTACCAAGACTGGCAAAATTGCTTAAAttct                                <  2:82230/250‑1 (MQ=255)                                                                                                                                                                                                                 catcaACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAAttcttc                              <  1:272545/251‑1 (MQ=255)                                                                                                                                                                                                                  atcaACCACGATATCATCGACAGGACACGGCATATGCTCCGCGCCCATCTCTTCCAGCACGTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTcg                             <  2:189827/251‑1 (MQ=255)                                                                                                                                                                                                                                          acacGGCATATGCTCCGCGCCCATCTCTGCCAGCACTTCTGCGGTATCGATATCAGTACCGATTGACAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACCCCCCCGGCACaa     <  2:334708/251‑1 (MQ=255)                                                                                                                                                                                                                                              ggCATATGCTCCGCGCCCATCTCTTCCAGCACTACTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTACGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTAACAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACCCCCCCGGCACAATCa  >  1:350265/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      AAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCACATGCTCCGCGCCCATCTCTTCCAGCACTTCTGCGGTATCGATATCAGTACCGATGGTCAAACGCAGCGGGAAATCGAAAATTTTCGGCAGCATCGCCGGGGCAATACACATAAAACCAAGCGGTTTTCCGGCCTGATGCATCGCTTGTGCCAGCGCCTTTAATTCACGGTCAACGGTGCATTCGCTACCAAGACTGGCAAAATTGCTTAAATTCTTCGCCGCGCCAAACCCCCCCGGCACAATCA  >  NC_000913/3349708‑3350194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑