Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,349,512	C→T	E100K (GAG→AAG)	mtgA ←	peptidoglycan glycosyltransferase MtgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,349,512	0	C	T	97.8%	138.6 / NA	47	E100K (GAG→AAG)	mtgA	peptidoglycan glycosyltransferase MtgA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (27/17);  minor base G (0/1);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAACGTTGTGCCGCAGCTTCGACGCCAAACACACCGTCGCCAAATTCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTCAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCAGCCAGGCGCTGACCTGTCGCTCGACCATTACCGCTGAGAAGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCG  >  NC_000913/3349267‑3349741                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       aTAACGTTGTGCCGCAGCTTCGACGCCAAACACACCGTCGCCAAATTCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATgg                                                                                                                                                                                                                                   <  2:23450/251‑1 (MQ=255) aTAACGTTGTGCCGCAGCTTCGACGCCAAACACACCGTCGCCAAATTCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATgg                                                                                                                                                                                                                                   <  1:279150/251‑1 (MQ=255)    aCGTTGTGCCGCAGCTTCGACGCCAAACACACCGTCGCCAAATTCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAg                                                                                                                                                                                                                                <  1:270822/251‑1 (MQ=255)            ccGCAGCATCGACGCCAAACACACCGTCGCCAAATTCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGCCCGTTTCTATCCTCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAGCCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGATAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTGATTG‑CGCTCGTTGTTCGCCAGGGCTTTCTTAATGGAAT‑CGACATCa                                                                                                                                                                                                                       <  2:69355/251‑1 (MQ=255)             cGCAGCTTCGACGCCAAACACGCCGTCGCCAAATTCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCa                                                                                                                                                                                                                       >  2:187235/1‑250 (MQ=255)             cGCAGCTTCGACGCCAAACACACCGTCGCCAAATTCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCa                                                                                                                                                                                                                       >  1:198460/1‑250 (MQ=255)              gCAGCTTCGACGCCAAACCCACCGTCGCCAAATTCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCaaa                                                                                                                                                                                                                     <  1:230948/251‑1 (MQ=255)                         cccAAACACTCCCTCGCCCAATTCGGAGATATTCAAGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TGTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCt                                                                                                                                                                                                          <  2:227525/250‑1 (MQ=255)                            aaaCACACCGTCGCCAAATTCGGCGATATTCAGGTAAACAGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTc                                                                                                                                                                                                         >  1:36404/1‑249 (MQ=255)                                             aTTCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGAtcttct                                                                                                                                                                                      >  1:62487/1‑251 (MQ=255)                                                    gATATTCAGGAAAACCGTAAGGATACGCTTTTTGCTCCAGACCGTTTCTATACCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATACCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACAGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGCAG‑CGCCATCAAAGCCCCAGTGCTCAGGAACTTTCTGATCTTCTGTGGCa                                                                                                                                                                                >  2:272545/1‑250 (MQ=255)                                                     atatTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTCTCACCAGCGTTAATCCGAATTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGAATTTTGCC‑TGACTGTTCAGAACTCGTCGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCCACGCCCCAGTGACCAGGAAATTTATGATCTTCTCCGGAAAt                                                                                                                                                                              >  1:256364/1‑251 (MQ=255)                                                            ggTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGcc                                                                                                                                                                       <  2:235981/251‑1 (MQ=255)                                                                 aCCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑Cg                                                                                                                                                                                                                             <  1:118007/192‑1 (MQ=255)                                                                 aCCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑Cg                                                                                                                                                                                                                             >  2:118007/1‑192 (MQ=255)                                                                   cGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTccc                                                                                                                                                                 >  1:232011/1‑250 (MQ=255)                                                                    gTCAGGATCCGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCCGCCCTTTTCAACCCCAGCTACGCCCATCCCATAAAAAGATATTTTTGGC‑TTTCTGTTGAGAAACCTTTGAAGCACCGC‑GAATACGTT‑TTTTATTG‑CGCTCTTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAACCCCCAGTTCTCAGGAATTTTCTTATCTTCGGCGGCAACCACCGCCAGTCCCAt                                                                                                                                                               <  1:35991/251‑1 (MQ=255)                                                                                     gCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGAt                                                                                                                                              >  2:220519/1‑251 (MQ=255)                                                                                                     aTCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTc                                                                                                                              >  2:275848/1‑251 (MQ=255)                                                                                                       cccccgctgaaaTCCGGCTTCCAGCTCTTTTCGACCCCACCTACGCCCATCCAAAAAAAAGAGTTTTTGGGC‑TGTCTGTTGAGAAATCGTGGAAGCACCGC‑AAATAGGGT‑TTTCATGG‑CGCCCGTTGGGCCCCAGGGTTTTCTTAATGGAAG‑CGACACAAAAGCCCCAGTGCTCAGGAAATTTCTGATCTCCTGCGGCACTCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTc                                                                                                                              <  2:232011/239‑1 (MQ=255)                                                                                                        cccAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCGCCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTc                                                                                                                              >  1:267694/1‑248 (MQ=255)                                                                                                         ccAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCGTAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCACAGGAAATTTCTGCTCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGa                                                                                                                           >  1:286075/1‑250 (MQ=255)                                                                                                           ggctttAATCCGGCTTCCAGCCCTTTTCGACCCCAGCTACGCCCAGCCCATAAAAGGAAATTTTTGGC‑GTTTTGTGGAGAAACGGTTGAACCCCCGC‑AAATAGGTT‑TTTCATTG‑CGCTCGTTGTGCGCAAGGGCTTTCTTAATGGAAG‑CGCAACCAAACCCCCAGGGCACAGGAAATTTCTTAGCTTCTCCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAAtg                                                                                                                        <  1:174123/247‑1 (MQ=255)                                                                                                             cGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGCAATCGTTGAAGCAACGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAAtgtg                                                                                                                      >  1:269136/1‑251 (MQ=255)                                                                                                                tAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGcc                                                                                                                    >  1:330453/1‑250 (MQ=255)                                                                                                                      ggCTTCCAGCCCTTTTCGCACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTTGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTAAGGAAATTTCTGATCTTCTGCGGCAATAACCGCCAGTCCCATCCACGGCGAGATTTGATCCCTACTGACCCAGTCAGAATGTGCCACGTAAc                                                                                                             >  2:313765/1‑251 (MQ=255)                                                                                                                                   ttcgtaacccgtcTACGCCCATCCCATAAAAAGAGTTGTGTGTC‑TGTGTGTTGAGAAATCGTCGAAGCAC‑GCATAATAGGGT‑TTTAATGG‑CGTTCGTTGTTCGCAGGGGCTTTCTTAATGGACG‑ACACATAAAACCCCCATTGCCAAGGAAATTTCGGTTTTTCCCCGGCAAGCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCag                                                                                                 <  2:6769/238‑2 (MQ=255)                                                                                                                                    ttCGAACCCAGCTCCGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCCCCGC‑GAATACGGT‑TTTCTTTG‑CGCTCGTTGTGCGCCAGGGTTTTCTTAATGGAAG‑CGACACCAAACCCCCAGTGCTCAGGAAATTTCTGATCTTCGGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATg                                                                                                <  1:414956/250‑1 (MQ=255)                                                                                                                                          cccAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCAGcc                                                                                           >  1:164723/1‑249 (MQ=255)                                                                                                                                                   gCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCAGCCAGGCGCTGAcc                                                                                >  2:129311/1‑251 (MQ=255)                                                                                                                                                     ccatcccatAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCAGCCAGGCGCTGACCTg                                                                              >  1:9027/1‑251 (MQ=255)                                                                                                                                                             aaaaaaagtaTTTTTGTG‑TGTCTGTTGAGAAATCGTTGAACCTCCGC‑GAATAGGTT‑TTTCAGGC‑CGCTCTTGGTGCGCCATGTGGTTCTGTATTGAGG‑CCATACTATAGCCCCAGTCCTCATGAATTTTCTGATCTGCTGCGTCACGCACCGCCAGTCCCATCCACGGCGAGATTTGTTCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCATCCAGGCGCTGACCTGTCgttc                                                                        <  2:286075/240‑4 (MQ=255)                                                                                                                                                                   gagaTTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCAGCCAGGCGCTGACCTGTCGCTCGACCAtt                                                                 >  1:277973/1‑250 (MQ=255)                                                                                                                                                                      aTTTTTGGC‑TGTCTGTTGAGAACTCGTTGAAGCACCGC‑GCATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCAGCCAGGCGCTGACCTGTCGCTCGACCATTACCg                                                             >  2:37431/1‑251 (MQ=255)                                                                                                                                                                        ttttGGCGTGTTTGTTGAGAAATCGTTGAAGCCCCGC‑AAATACGTT‑TTTCATTG‑CGCTCTTTGGGCGCCAGGGCTTTCTTAAGGGAAG‑CGAAATAAAAGCCCCAGTGCGCAGGAATTTTCTGATCTTCTGCGCCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCAGCCAGGCGCTGACCTGTCGCTCGACCATTACCg                                                             <  1:107710/250‑1 (MQ=255)                                                                                                                                                                         tttGGC‑TGTCTGTTGAAAAATCGTTGAAGCACCGC‑AAATACGTT‑TTTCATTG‑CGCTCTTTGGGCGCTGGGGTTTTTTAAATGGAAG‑CGACATCAAAGCCCCAGTGCCCAGGAAATTTCTGATTTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCAGCCAGGCGCTGACCTGTCGCTCGACCATTACCGCt                                                           <  1:220519/250‑1 (MQ=255)                                                                                                                                                                          tttggtgtttgttgtagaAACCGTTGAAGCACCGC‑AAGTCCGGT‑TTCCATTG‑CGCTCTTTGTGCGCCAGGGCTTTCTTAATGGACG‑CGACATAAAAGCCCCGGGGCTCAGAAAATTTCTGAGTTTCTGGGCCAACCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCAGCCAGGCGCTGACCTGTCGCTCGACCATTACCGCt                                                           <  2:53160/235‑1 (MQ=255)                                                                                                                                                                                      ttGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAGTTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCAGCCAGGCGCTGACCTGTCGCTCGACCATTACCGCTGAGAAGGGAAc                                                >  1:344730/1‑249 (MQ=255)                                                                                                                                                                                      gtGAGAAATCGTTGAAGCCCCGC‑GAAT‑CGGTGTTACTTTG‑CGCGCGTTGTGCGCCGGGGCTTTCT‑TATGGAAGGCGACAACAACGCCCCAGTGCCCAGAAATTTTCTGATCTTGTGCGGCAATCTCCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCAGCCAGGCGCTGACCTGTCGCTCGACCATTACCGCTGAGAAGGGAACAg                                              <  2:136623/250‑1 (MQ=255)                                                                                                                                                                                           aaaTCGTTGAAGCCCCGC‑GAATACGTT‑TTTCATTG‑CGCCCGTTGGGCGCCGGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGAAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCa                                                                                              <  1:159899/198‑1 (MQ=255)                                                                                                                                                                                           aaaTCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCa                                                                                              >  2:159899/1‑198 (MQ=255)                                                                                                                                                                                                        aCCGC‑GAATACGGT‑TTTCATTG‑CGCTAGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGACTGTGCCACGTAACGAAAATTGCCATGCAGCCAGGCGCTGACCTGTCGCTCGACCATTACCGCTGAGAAGGGAAAAGGCGCAACGCTAACCAAcg                            >  2:284893/1‑251 (MQ=255)                                                                                                                                                                                                                 tACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCAGCCAGGCGCTGACCTGTCGCTCGCCCATTACCGCTGAGAAGGGAACAGGCGCAACGCTAAACAACGCGATAccc                    >  1:222198/1‑251 (MQ=255)                                                                                                                                                                                                                   ccgT‑TTTTATTGGCGCTCGTTGGGCGCGAGGGTTTTCTTAATGGAAG‑CGAAACAAAACCCCCAGTGCTCAGGAAATTTCTGATCTTCGGCGGCAACCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCAGCCAGGCGCTGACCTGTCGCTCGACCATTACCGCTGAGAAGGGAACAGGCGCAACGCTAAACAACGCGATGcccc                   <  1:129311/249‑1 (MQ=255)                                                                                                                                                                                                                         ttCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTTACTGGCAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTACCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCAGCCAGGCGCTGCACTGTCGCTCGACCATTACCGCTGAGAAGGGAACAGGCGCAACGCTACACAACGAGATGCCCCCGcccc             >  2:392932/1‑251 (MQ=255)                                                                                                                                                                                                                              tG‑CGCTCGTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCAGCCAGGCGCTGACCTGTCGCTCGACCATTACCGCTGTGAAGGGAACAGGCGCAACGCTCAACACCGCGATGCCCCCGCCCCAGaa         >  2:297102/1‑250 (MQ=255)                                                                                                                                                                                                                                      gTTGTGCGCCAGGGCTTTCTTAATGGAAG‑CGACATCAAAGCCACAGTACTCAGGAAATTTCTGATATTCTGCGGCAATCACAGCCGGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGCGCCACGTAACGAAAATTGCCATGCAGCCATGCACTGACCTGTCGCTCGACCATTCCCGCTGAGACGGGAACAGGCGCAAAGCTAAAAAACGCTATGCCCCCGCCCCAGAAAACgggg  >  2:228051/1‑248 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       ATAACGTTGTGCCGCAGCTTCGACGCCAAACACACCGTCGCCAAATTCGGCGATATTCAGGTAAACCGTCAGGATACGCTTTTTGCTCCAGACCGTTTCTATCCCCAGCGTTAATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGC‑TGTCTGTTGAGAAATCGTTGAAGCACCGC‑GAATACGGT‑TTTCATTG‑CGCTCGTTGTGCGCCAGGGCTTTCTCAATGGAAG‑CGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACCCAGTCAGAATGTGCCACGTAACGAAAATTGCCATGCAGCCAGGCGCTGACCTGTCGCTCGACCATTACCGCTGAGAAGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCG  >  NC_000913/3349267‑3349741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑