Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,321,703	G→A	intergenic (‑90/+369)	yhbX ← / ← leuU	putative hydrolase YhbX/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,321,703	0	G	A	100.0%	131.0 / NA	38	intergenic (‑90/+369)	yhbX/leuU	putative hydrolase YhbX/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/15);  total (23/15)

AACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAGAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCG  >  NC_000913/3321464‑3321945                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   aaCCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATGTGTTGAATACTGTCAGCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACt                                                                                                                                                                                                                                         <  1:205409/251‑1 (MQ=255)           gTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGttttt                                                                                                                                                                                                                               >  1:10097/1‑251 (MQ=255)                aaCGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAAGTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCttt                                                                                                                                                                                                                          <  2:150542/251‑1 (MQ=255)                                         atatGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCTACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTtct                                                                                                                                                                                                 <  1:247861/251‑1 (MQ=255)                                                 tCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAAtt                                                                                                                                                                                         >  1:79546/1‑251 (MQ=255)                                                    ggAAGAAGCGACTAAGTGCGTAATACCAAAAACAATAACACAAATATACCACAACCAATGAGAGTTAAAAGTACGAGCAAAATTGTTGAATACTGTCATCCAGAAAGCCAAATTCACATCCAATATAAAAAATTATCAACAATTTTTAATTTTATATCATACATGTTTTTCAACTCTGATCCATATCAATACGAAACACTGTTAGTTTTTTCTTTTTAAATGTTGCGGCCTTTGTTCTCTCAGTAATTaaa                                                                                                                                                                                      <  2:75662/251‑1 (MQ=255)                                                      aagaagCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAAt                                                                                                                                                                                     >  1:149369/1‑250 (MQ=255)                                                       agaatCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCATAAATCTAAGGTCACAACCAATATAAACAATTATCAACAATGGTTAATGTTACAGCAGACATTTGTTTCAACGCGGATCCATAGCAAAGCGAAACGCTGGTAGTGTAATCGTTTTAAAGGATGCAGCCATTGCGTTCTCAGTAATTAAATaa                                                                                                                                                                                   <  2:405793/251‑1 (MQ=255)                                                        gaagCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAAc                                                                                                                                                                                  <  1:276931/251‑1 (MQ=255)                                                                          aTACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGATCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGACACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGAGTTCTCAGTAATTAAATAACATTATCAATGCGTctct                                                                                                                                                                 >  2:349183/1‑250 (MQ=255)                                                                               aaaaaCAATAACACAAAGATACAACAACCAATGAGGTTTAAAAGATCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTATCAGTAATTAAATAACATTATCAATGCGTCTCTAAACAt                                                                                                                                                           >  2:50404/1‑251 (MQ=255)                                                                                          cacaAAGAGACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTGTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTGTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACg                                                                                                                                                <  1:276414/251‑1 (MQ=255)                                                                                             aaaGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAAc                                                                                                                                                                                                                                                 <  1:167004/151‑1 (MQ=255)                                                                                             aaaGATACAAAAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAAc                                                                                                                                                                                                                                                 >  2:167004/1‑151 (MQ=255)                                                                                               aGATACAACCACCAATGAGACATACAAGTTCTAGCAAATTTGTTGAATCCTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTATTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGATCTCAGTAATTAAATAACATTATCAATGCGTCTCTCAACATTACGCCGAAAGTAGcc                                                                                                                                           >  2:18926/1‑251 (MQ=255)                                                                                                        aacCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGt                                                                                                                                  >  1:295072/1‑251 (MQ=255)                                                                                                               gagaTTTAAAAGTTCTAGCAAAATTGTTGAATACTGTCAACCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACt                                                                                                                            <  2:149369/250‑1 (MQ=255)                                                                                                                 catttcagaGTTATAGCCAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAATTGATGCGGCCATTGAGCTCTCAGTAATTAAATAACATTATCTATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTCTACTGAc                                                                                                                         >  2:19166/9‑251 (MQ=255)                                                                                                                            tCTAGAAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTACACATTACGCAGAACGTCGCAATCAGACGTTATACTGACAGGAGCAGGAt                                                                                                              >  1:53582/1‑251 (MQ=255)                                                                                                                                  aaaTTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGttt                                                                                                         >  1:101782/1‑250 (MQ=255)                                                                                                                                        gttgAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCgaga                                                                                                  <  1:202098/251‑1 (MQ=255)                                                                                                                                        gttgAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCg                                                                                                     >  1:122968/1‑248 (MQ=255)                                                                                                                                                        cAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCATGCTGGTTTATCGAGAAGTAAACATATCCGt                                                                                   >  2:284707/1‑250 (MQ=255)                                                                                                                                                                cAAGTTCACAACCAATATAAAACGTTATCCAATCTGCTTAATTTTATATCAGCCATGTTTATCAAATCGGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTATAAGTAATTAAATAACATTATCAATGAGACTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGAGGGTTTATCGAGAAGAAAAAATATCCGTATTCGTAcc                                                                          >  2:218103/1‑251 (MQ=255)                                                                                                                                                                    ttCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATACATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTCATTAAATAACATTATCAATGCGTCTATAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCgaa                                                                       >  1:322011/1‑248 (MQ=255)                                                                                                                                                                        caACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTgg                                                                  <  1:375266/251‑1 (MQ=255)                                                                                                                                                                                              acaaTGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCa                                             >  1:370135/1‑250 (MQ=255)                                                                                                                                                                                                 aTGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTa                                         <  2:79546/251‑1 (MQ=255)                                                                                                                                                                                                     ttAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTc                                     >  1:323437/1‑251 (MQ=255)                                                                                                                                                                                                        aTTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGt                                  <  1:143284/251‑1 (MQ=255)                                                                                                                                                                                                         ttttATATCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATACGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGt                                  >  1:337667/1‑250 (MQ=255)                                                                                                                                                                                                            tataTCAGACATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGa                               >  1:12048/1‑250 (MQ=255)                                                                                                                                                                                                                     cATGTTTTTCCACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATcc                                                                                     >  2:281884/1‑187 (MQ=255)                                                                                                                                                                                                                     cATGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATcc                                                                                     <  1:281884/187‑1 (MQ=255)                                                                                                                                                                                                                      aTGTTTTTCAACTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAAtt                    >  1:10552/1‑251 (MQ=255)                                                                                                                                                                                                                               aaCTCTGATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGCTGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGAGTCTCTAACCATTACGCAGAACGTCGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTa            >  1:370352/1‑250 (MQ=255)                                                                                                                                                                                                                                     gATCCATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCAGTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTa     <  2:89451/251‑1 (MQ=255)                                                                                                                                                                                                                                        ccATATCAAAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCg  >  1:314979/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   AACCAGGATGGTCAGAACGAAGAACAGCAAGCGCTGAACCATATGCGCTCCGGAAGAAGCGACTAAGTTCGTAATACCAAAAACAATAACACAAAGATACAACAACCAATGAGATTTAAAAGTTCTAGCAAATTTGTTGAATACTGTCATCCAGAAATCCAAGTTCACAACCAATATAAACAATTATCAACAATGTTTAATTTTATATCAGACATGTTTTTCAACTCTGATCCATATCAGAACGAAACACTGTTAGTTTTTTCTTTTTAAATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCG  >  NC_000913/3321464‑3321945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑