Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,178,904	G→A	D264N (GAT→AAT)	tolC →	outer membrane channel TolC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,178,904	0	G	A	100.0%	183.3 / NA	60	D264N (GAT→AAT)	tolC	outer membrane channel TolC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/32);  total (28/32)

ACCGTGCTGGCGAACGAAGTGACCGCACGTAATAACCTTGATAACGCGGTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGC  >  NC_000913/3178667‑3179146                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    aCCGTTCTGGCGAACGAAGTGACCGCACGTAATAACCTTGATAACGCGGTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGGCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTAcc                                                                                                                                                                                                                                        <  2:252244/251‑1 (MQ=255) aCCGTGCTGTAGAACGAAGTTACCGAACGTCATAACCTTGATAACGCGGTAGATAAGCTGTGCTAGATCACCGGCAACTACTATCCGGTACGGGCTGCGCTGAATGTCGAAAACGTTTAAACCGACAACCCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCCACCTGTCGCTGTTTCAGTCACGCTTGAGCCAGGACCTGGCGCGTGAGCAAATTCGCCAGTCGCAGAATGGGCACTTAc                                                                                                                                                                                                                                         <  2:385023/250‑1 (MQ=255) aCCGTGCTGGCGAACGAAGTGACCGCACGTAATAACCTTGATAACGCGGTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTAcc                                                                                                                                                                                                                                        <  1:124690/251‑1 (MQ=255) aCCGTGCTGGCGAAAGAAGGGACCGCACGTGATAACCGTGATAACGCGGGAGAGCAGCGGCGCCAGATCACCGGTAACTACGATCCGGAACTGGCTGCGCTGAATGTCGAAAGCTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTTGCTGTTACAGGCACGGTTGAGCCAGGCCCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGGCACTTAcc                                                                                                                                                                                                                                        <  2:38579/251‑1 (MQ=255)     ggCTGGCGAACGAAGTGACCGCACGTAATAGCCTTGATAACGCGGTAGAGCAGCTGCGCCAGATCACCGGTAACGACTATCCGGAACTGGCTGCGGTGAATGTCGAAAACTGTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGAct                                                                                                                                                                                                                                    <  1:254913/250‑1 (MQ=255)               cgaaGTGACCGCACGTAATAACCTTGATAACGCGGTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTaa                                                                                                                                                                                                                          <  1:391851/251‑1 (MQ=255)                        cGCACGTAATAACCTTGATAACGCGGTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCt                                                                                                                                                                                                                  >  2:404857/1‑250 (MQ=255)                         gCACGTAATAACCTTGATAACGCGGTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTAc                                                                                                                                                                                                                >  1:17422/1‑251 (MQ=255)                                       tGATAACACGGTAGAGCAGCTGCGCCAGATCACCGGTAAATACTATCCGTAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAACACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCgggcgttcttgc                                                                                                                                                                                                   >  2:144802/1‑241 (MQ=255)                                        gATAACGCGGTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGaca                                                                                                                                                                                                  <  1:169946/250‑1 (MQ=255)                                                ggTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTtat                                                                                                                                                                                          >  1:348200/1‑250 (MQ=255)                                                 gTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAg                                                                                                                                                                                        <  1:46369/251‑1 (MQ=255)                                                     agcagcTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGCTTTAACGGCTTCTACCGGGATTTCTTACACCCCTTATAGCGGt                                                                                                                                                                                    >  2:90627/1‑251 (MQ=255)                                                        agcTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTAAAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGACACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCg                                                                                                                                                                                 >  1:182490/1‑251 (MQ=255)                                                                    tCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCCCTTATAGCGGTTCGAAAACCCgtggg                                                                                                                                                                     >  2:153559/1‑250 (MQ=255)                                                                      aCCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGc                                                                                                                                                                   >  1:122085/1‑251 (MQ=255)                                                                             actattATCCGTAGCTGGCTGCGCTGAATTTCGAATACTTTAAAACCTACAAACCCGAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAAGGCAAGCGGTCGCTGTGACAGGCACTCGTGAGCCAGGACCGGGCGCGAGAGCAAAATCGCCAGTGGCATAATGGTCTCTTACCGTCTCTGGATTTAACGGTTTCTACCGGGATTTTTGATACCTCTTGTTGCGGTTGGAAATCCGGTGGTGCCGCttgt                                                                                                                                                            <  2:373307/251‑4 (MQ=255)                                                                                actaTCCGGAACTGGCTGCGCTGAAGGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCGGGAGTTAACGGCTTCTACCGGGATTTCTGACACCTCTGATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTa                                                                                                                                                           <  2:22868/249‑1 (MQ=255)                                                                                     ccGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCGGGCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGt                                                                                                                                                     <  2:199293/250‑1 (MQ=255)                                                                                      cGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTTCCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTAt                                                                                                                                                   <  1:242320/251‑1 (MQ=255)                                                                                        gAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGTACCTGGCGCGCGAGCAACTTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGa                                                                                                                                                                                                           >  2:12628/1‑193 (MQ=255)                                                                                        gAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGa                                                                                                                                                                                                           <  1:12628/193‑1 (MQ=255)                                                                                        gAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATg                                                                                                                                                  >  1:81497/1‑250 (MQ=255)                                                                                                 gcgcTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGc                                                                                                                                          <  1:122253/249‑1 (MQ=255)                                                                                                  cgcTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCGGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGAATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAa‑t                                                                                                                                      <  1:283976/251‑1 (MQ=255)                                                                                                          gtagAAAACCTTAAACCCGACAAACCCCAGCCGGTTAACGCGCTGATGAAAGAAGCTGAAAAACGCAACCGGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTTCCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCTGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGTTGATAGCAA‑TAGGGGcc                                                                                                                               <  2:7660/247‑1 (MQ=255)                                                                                                           tCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGcc                                                                                                                               >  1:276996/1‑249 (MQ=255)                                                                                                            cGAAAACTTTAAAGCCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGACGCTGTTACAGGCACGCTTGTGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAACTCTTATAGAGGTTCTAAAACCCGTGGTGCCGCTGGTACCCatgaagaacatagctatatggcac                                                                                                                                >  2:1120/1‑225 (MQ=255)                                                                                                                   tttAAAACCGCCAAACAACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACCCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTATGACACCTCTTATAGCAGTTCGAAACCCCGAGCTGCAGCTGGTACCCAGGATGACGATAGGAACTA‑GGGCCAGaaacacg                                                                                                                      >  2:366049/1‑245 (MQ=255)                                                                                                                       aaaCCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGc                                                                                                                 <  1:270410/251‑1 (MQ=255)                                                                                                                       aaaCCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGAAACCTGCCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGACGCTGGGACCCAGTATGACGATAGCAA‑TATTGGACAGAACAAAGTTGGc                                                                                                                 >  2:413594/1‑251 (MQ=255)                                                                                                                           cGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGGCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGa                                                                                                             <  1:412600/251‑1 (MQ=255)                                                                                                                              cAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCCGAGCt                                                                                                          >  1:138923/1‑251 (MQ=255)                                                                                                                               aaaCCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCtt                                                                                                         <  1:44192/251‑1 (MQ=255)                                                                                                                                  ccACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTtctc                                                                                                      <  1:9545/251‑1 (MQ=255)                                                                                                                                   cacaGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCg                                                                                                     <  1:318763/251‑1 (MQ=255)                                                                                                                                      aGCCGGTTAACGCGCTGCTGAAAGCAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTCTAGCGGTTCGAAAACCCGTGGTGCCCCTGGTACCCAGTATGACGATAGCAA‑TATGGGACAGAACAAAGTTGGCCTGCGCTTCTCACTg                                                                                                  >  1:153073/1‑251 (MQ=255)                                                                                                                                      aGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTg                                                                                                  >  2:361633/1‑251 (MQ=255)                                                                                                                                      aGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTg                                                                                                  <  1:333444/251‑1 (MQ=255)                                                                                                                                      aGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTg                                                                                                  <  1:412222/251‑1 (MQ=255)                                                                                                                                           gTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGAt                                                                                             <  1:228016/251‑1 (MQ=255)                                                                                                                                                  cgcTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATc                                                                                        >  1:9100/1‑249 (MQ=255)                                                                                                                                                  cgcTGCTGAAAGAAGCCGAAAAACGCAACCGGTCGCTGTTACAAGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCGA‑TATGGGCCCGAACAAAGTTGGCATGAGCTTCTCGCTGCCGATTTATc                                                                                        >  1:134016/1‑249 (MQ=255)                                                                                                                                                          caagaagCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAAt                                                                              >  2:12034/2‑251 (MQ=255)                                                                                                                                                            agaagCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATgg                                                                            <  2:68743/251‑1 (MQ=255)                                                                                                                                                                gCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGCACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATgggta                                                                         >  2:63513/1‑247 (MQ=255)                                                                                                                                                                  cGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACt                                                                      <  1:400063/251‑1 (MQ=255)                                                                                                                                                                                   cGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATCCGCCAGGCGCAGAATGGTCCCTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGca                                                     <  2:123801/251‑1 (MQ=255)                                                                                                                                                                                            aGGCACGCTTGAGCCAGGACCTGGCACGCGCGCAAATTCGCCAGGCGCAGACTGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTTACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGAAAGCAA‑TATGGGCCAGAAAAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGGGAAACAGGCACAGTACAAc                                            >  2:363495/1‑251 (MQ=255)                                                                                                                                                                                             ggCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCAACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACt                                           <  1:222359/251‑1 (MQ=255)                                                                                                                                                                                                             gACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGa                           <  2:314856/251‑1 (MQ=255)                                                                                                                                                                                                              aCCTGGCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCCCAGTAAAACTTTGTCGGTGCCAGCg                            >  1:226380/1‑249 (MQ=255)                                                                                                                                                                                                                  ggCGCGCGAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCGCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCCTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCa                        <  1:155362/249‑1 (MQ=255)                                                                                                                                                                                                                    cgcgcgAGCAAATTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGGGCCAGCGAGCAACTg                    >  2:202796/1‑251 (MQ=255)                                                                                                                                                                                                                             aaaTTCGCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGcc           <  1:101372/251‑1 (MQ=255)                                                                                                                                                                                                                               aTTCGCCAGGCGAAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGccc          >  1:60225/1‑250 (MQ=255)                                                                                                                                                                                                                                   gCCAGGCGCAGAATGGTCACTTACCGCCTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTACCGCTGGTACCCAGTATGACGATAGCAA‑TCTGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTTAAACAGGACCAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCAt        >  2:82656/1‑248 (MQ=255)                                                                                                                                                                                                                                   gCCAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGt     >  1:47142/1‑251 (MQ=255)                                                                                                                                                                                                                                    ccAGGCGCAGAATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTAGAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCGGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTa    <  1:251707/251‑1 (MQ=255)                                                                                                                                                                                                                                      aGGCGCAGAATGGTCACTTACCGACTCTGGATTTACCGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGATGGTACCCCGTATGACGATAGCAA‑TATAGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCCCAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGCAGc  >  2:64177/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    ACCGTGCTGGCGAACGAAGTGACCGCACGTAATAACCTTGATAACGCGGTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAA‑TATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGC  >  NC_000913/3178667‑3179146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑