Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,974,399	C→T	G539E (GGA→GAA)	aas ←	fused 2‑acylglycerophospho‑ethanolamine acyltransferase/acyl‑acyl carrier protein synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,974,399	0	C	T	96.4%	169.4 / NA	57	G539E (GGA→GAA)	aas	fused 2‑acylglycerophospho‑ethanolamine acyltransferase/acyl‑acyl carrier protein synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (24/30);  minor base G (0/2);  total (24/33)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCAT‑ACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACA  >  NC_000913/2974152‑2974639                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 gCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCCAAACGCACAATATCGCCAGTGTCAT‑ACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCCGAACACCTGGCTTCTCCACCCCCAGATAACCGTTCATTATGTTCGGCCCTTTAAGTTGCAGGCTTCCGCCCTCTTCGATACCAGGGACCCCCAACAGGCGCGCATCCATTTCTg                                                                                                                                                                                                                                               >  2:232457/1‑251 (MQ=255)     gCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCAT‑ACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCAGCCATTTCTGGt                                                                                                                                                                                                                                             <  1:58954/249‑1 (MQ=255)        aTTTTGGCAAAGCGTTTGGCGCGGCCCTGAATCTGCCCAAAGCCCTGCACGTCAAAACGCACAATAGCGCCAGTGTCAT‑ACCAGCCGCGCGCCAGTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCCCCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTGTCAGGCGTTCGCCCTCTTCGATACCAGGGACCGACATCAAGCGCGCATCCATTGCGGGTAGAAt                                                                                                                                                                                                                                        <  2:136712/251‑1 (MQ=255)                 aaGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCAT‑ACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGTTGGGCACTTCCAGTACACCTGGCTGCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCtac                                                                                                                                                                                                                               <  1:270171/251‑1 (MQ=255)                 aaGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCAT‑ACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACCCCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCCGGCGTCCGCCCTCTTCGATACCAGGGACCGACACCAGGCGCGCATCCCTTTCTGTCAGAAAACGCCCta                                                                                                                                                                                                                                >  2:13256/1‑250 (MQ=255)                                    gAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCAT‑ACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCgccgcc                                                                                                                                                                                                            <  1:296857/251‑1 (MQ=255)                                            cccatgCCCTGCCCGCCAAAACGCCAAATATCCCCAGCG‑CATTACCAGCCGCGCTCCATCGCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACCACTGGCTTCTCCACCCGCAGATAGCCGTACAATATGTTCGGCCCTTTCAGTCGCAGGCGTCCTCCCGCTTCGATACCAGGGACCGACAACAGGCGTGCATCCATTTCTGGCAGAACGCGCCCTACCGTACCGGTTTTCGCCGCTATCTGTAc                                                                                                                                                                                                    <  2:293895/246‑1 (MQ=255)                                                            aaaaaCGCACAATATCGCCAGTGTCAT‑CCCAGCCGCGCTCCATTCCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGAc                                                                                                                                                                                     <  2:340086/249‑1 (MQ=255)                                                                  gCACAATATCGCCAGTGTCAT‑ACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCCTTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGgcgcg                                                                                                                                                                              >  1:347750/1‑251 (MQ=255)                                                                  gCACAATATCGCCAGTGTCAT‑ACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGCAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGCAACGCCAGgcgcg                                                                                                                                                                              >  1:264079/1‑251 (MQ=255)                                                                       atatCGCCAGTGTCAT‑ACCAGCCGCGCTCCATTTCGCCGCGAACATTCGCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTc                                                                                                                                                                         <  2:123866/251‑1 (MQ=255)                                                                           cGCCAGTGTCAT‑ACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGATTCTCCCCCCGCAGATAGCCGTTCATTCTGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCCACAACAGGCGCGCATCCATTTCTGGTAGCATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTc                                                                                                                                                                     >  1:408375/1‑251 (MQ=255)                                                                            gCCAGTGTCAT‑GCCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGCGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGTCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGTCGCGCATTCGGTCa                                                                                                                                                                    <  1:237684/251‑1 (MQ=255)                                                                              cAGTGTCAT‑ACAAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATAAGCCCTACCGTACCGGGGTTCGCCGCCATCGGTCCGTTGATAGAAACGACAAGCGCGCCTTCGGTCAcc                                                                                                                                                                  >  2:370607/1‑250 (MQ=255)                                                                                        aCCAGCCGCGCTCTATTTCGCCGCGAACATTCGCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTGCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACTCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATGCGGTCACGCCGTAGcc                                                                                                                                                          <  1:318509/250‑1 (MQ=255)                                                                                         ccAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAACCGTTCATTATGATCGACCCTTTCAGTTGCAGGCGTCCGCCCTCCTCGATACCAGGGACCGACAAAAAGCGCGCATCCATTTCTGGTTGAATACGACCTACCGTACCGGGTTTCGACGCCATCGGGACGTTGGTAGAAACGACAGGCGCTCATTCGGTAACGACGttacctt                                                                                                                                                        >  1:56746/1‑245 (MQ=255)                                                                                          cAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTTTGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTCCCGGGTTTCGCCTCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTc                                                                                                                                                       >  2:348509/1‑251 (MQ=255)                                                                                           aGCCGCGCTACATTTCGCCGCGAAAATTCTCGGCGGAGGGAACTTCCCGCACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACATGCCAGCATACCTTTCTGGGAAAATACGACCTACCGGACCGGGTTTCGCCGCCATCGGTACGTTGATTGAAACAACCGGCGCGCATTctgggacgccggtgcctta                                                                                                                                                       >  2:291657/1‑232 (MQ=255)                                                                                            cccGCGCTCCAATTCGCCGCGAACATTCTCGGCTGTGGGCACTTCCAGTACTCCGGGCTTCTCCCCCCGCAGATAGCCGTTCATTATGTTCTGCCTTTTCAGTTGCTGGCGGCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCCTTCCTGGTAGAATACGCCCTACCGTACCTGGTTTCGCCGCCATCGGTACTTTTATAGAAACTACAGGCGCGCATTCGGTCACGCCGTAGCCTTCaa                                                                                                                                                     <  2:231077/250‑1 (MQ=255)                                                                                             ccGCGCTCCAGTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAg                                                                                                                                                    <  1:232457/251‑1 (MQ=255)                                                                                              cgcgCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCCGTAAACATGGCTTCTCCCCCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGCATACGCCCTACCGTACCGGGTTTCCCCGCAATCGGTACGTTGCTCGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAgg                                                                                                                                                   >  1:23214/1‑251 (MQ=255)                                                                                                          cgccgcGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCTCCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCGGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCa                                                                                                                                       <  1:38866/251‑1 (MQ=255)                                                                                                                gAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGCCCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCCTCGGTACGTTGATAGAAACGACCAGCGCGCATTCGGTCCCGCCGTAGCCTTCAAGGATGCGCCGGCCCAAttt                                                                                                                                  >  2:219500/1‑250 (MQ=255)                                                                                                                      tctcGGCGGTGGGCACTTCCAGTACCCCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATGCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTg                                                                                                                            <  1:123076/250‑1 (MQ=255)                                                                                                                      tctcGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTg                                                                                                                            <  1:415250/250‑1 (MQ=255)                                                                                                                           gcggTGGGCACTTCCAGTACACATGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATATAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAg                                                                                                                      <  2:27369/251‑1 (MQ=255)                                                                                                                             ggTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGACCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCt                                                                                                                    >  1:193222/1‑251 (MQ=255)                                                                                                                                  gCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTg                                                                                                               >  2:37282/1‑251 (MQ=255)                                                                                                                                  gCACTTCCAGCACTCCTGGCTCCTCCACCTGCAGATAGCCTTTCAATATGTTCGGCCCGTTCAGTTGCAGTCGTCAGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCTGACCGTGTTTCGCCGCCCTCGGTACTTTTACATAAACGACAGGCCCGCTTTCTGCCACTCCGTAGCCTTCAAGGATGCTCAGGCCAAATTTATCCTGCCAAATCTGTTTg                                                                                                               <  2:25774/251‑1 (MQ=255)                                                                                                                                      ctCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTACGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGACTACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGCGACGACAGGCGCGCCTTCGGTCACGCCGTCGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCAAACGCTGTTTGGTAc                                                                                                           >  2:119920/2‑251 (MQ=255)                                                                                                                                       tcaattACACCTGGTTTCTCCACCCGCACATAGCCTGTCAGTATGGTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATGCCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCGACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCTCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTCTCCTGCCAAAGCTGTTTGGt                                                                                                             <  2:112036/243‑1 (MQ=255)                                                                                                                                        ccAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCAAAAGCTGTTTGGTACtt                                                                                                         >  2:346983/1‑251 (MQ=255)                                                                                                                                         cAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCACCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAACTTTATCCTGCCAAAGCTGTTTGGTACttt                                                                                                        >  1:256305/1‑251 (MQ=255)                                                                                                                                                   ggCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCTTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGAATCCATTTCTGGTAGAATACGCCCTACCGTACCGGTTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTTGTACGTTCTTGTAAttt                                                                                              <  2:307734/251‑1 (MQ=255)                                                                                                                                                   ggCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCGCGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAAtt                                                                                               <  1:243621/250‑1 (MQ=255)                                                                                                                                                   ggCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAAtt                                                                                               <  1:104923/250‑1 (MQ=255)                                                                                                                                                       tctcCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCt                                                                                          >  2:256608/1‑251 (MQ=255)                                                                                                                                                        ctcCCCCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGt                                                                                                             <  2:260901/231‑1 (MQ=255)                                                                                                                                                        ctcCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGt                                                                                                             >  1:260901/1‑231 (MQ=255)                                                                                                                                                              ccGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTg                                                                                   <  1:324472/251‑1 (MQ=255)                                                                                                                                                                        ccGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCCCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATa                                                                         <  2:288751/251‑1 (MQ=255)                                                                                                                                                                          gTTCATTATGTTCGGCCCTTTCAGTTGCCGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCACAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACCTAGc                                                                       >  2:400652/1‑251 (MQ=255)                                                                                                                                                                                 atgatCGGCACTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACATGGGGCCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTAAGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCCTCAAGGATGAGCAGGCCAACTTTATCATGCCAAAGCTGTTTGGTACTTTGTTGTAATTTTTTCGCGCCTGccacgactctgcgtcgacg                                                                >  2:80293/5‑236 (MQ=255)                                                                                                                                                                                 aTGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATCCCAGGTACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTGCCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAGATTTATCCTGCCAAAGCTGTTTGGTAGTTTCTTGTAATTTTTCAGCGTCTGCCACCACATAGCGTAGAc                                                                 <  2:167871/250‑1 (MQ=255)                                                                                                                                                                                      cGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGa                                                           <  1:219500/251‑1 (MQ=255)                                                                                                                                                                                        gCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAg                                                         <  1:348509/251‑1 (MQ=255)                                                                                                                                                                                              tCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCTCAGTCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTGGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATa                                                    <  1:278064/250‑1 (MQ=255)                                                                                                                                                                                               cAGTTCCAGGCGTCCGCCCACTTCGCTACCAGGGACCACCAACAGGCGCGCCTCCATTTGCGGTCGAACACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTCAATAGAAACGACAGGCGAGCCTTCAGTCACGCCGTAGCCTACAAGGATGCGCAGGCACAATTTATCCTGCCGACGCTGTATGGTACTTTCTTGTATTTTTTATGCGCCTGCCACCACATAGAGTAGACGATAGAAGCCATACg                                                  >  2:216831/1‑251 (MQ=255)                                                                                                                                                                                                        ccGTCCGCCCTCTTCGTTACCAGGGACCGACAACAGGGGCGCATCCATTGCTGGTAAAATACACCCTACCTTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACATGCGCGCATTCGGGCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCTTGCCACCACATAGCGTATACGATAGAAGTCATACGAGTTGGCGa                                         <  2:399312/250‑1 (MQ=255)                                                                                                                                                                                                          gtccgccctctctgttaccatgtcccGACACCAGGCGCGCCTCCCCTTCTGGTATAATCCGCCCTACCGTCCCGTGTTTCGCCGCCCTCGTTACGTTGATAGAAACGACAGCCGCCCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAATTTTATCCTGCCAAAGCTGTTTGGTTCTTTCTTGTAATTTTTCTTCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATCCGGGTTGGCTaaa                                       <  2:307925/227‑1 (MQ=255)                                                                                                                                                                                                                                   cGACAACAGACGCGCATTCATTTCTGGTAGAATACGACCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTAGTTTACGCCGTAGCCTTCAAGGATTCGCAGTCCAATTTTATCATGTAAAAGCTGTTTGGTACTCTCTTGTATTTTTTCTGCGCCGTCAACCCCAGAGCGTAGAAGATAGAAGTCATAAGGATTGTAGAAACGCGCGTAGAGAGCGAGGCAAGta               >  2:228866/1‑249 (MQ=255)                                                                                                                                                                                                                                     acaacaGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCAGACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAg            <  2:347750/251‑1 (MQ=255)                                                                                                                                                                                                                                     acaacaGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCAGCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCCCGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTTCCCCCACATAGCGTAGACGATAGAAGTCATACGGGTTGTCGAAACGCGCGTAGTGACCGAGGAAAGTCGAg            <  1:165290/251‑1 (MQ=255)                                                                                                                                                                                                                                       aacaGGCGCGCTTCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCTCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGt          <  2:317828/251‑1 (MQ=255)                                                                                                                                                                                                                                       aacaGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGt          <  1:293305/251‑1 (MQ=255)                                                                                                                                                                                                                                        acaGGCGCGCATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTg         >  1:230852/1‑251 (MQ=255)                                                                                                                                                                                                                                                gcATCCATTTCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGaaca  >  1:147463/1‑250 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 GCCTGCAATTTTGGCAAAGCGTTTTGCGCGGCCCTGAATCTGCACAAAGCCCTGCTCGTCAAAACGCACAATATCGCCAGTGTCAT‑ACCAGCCGCGCTCCATTTCGCCGCGAACATTCTCGGCGGTGGGCACTTCCAGTACACCTGGCTTCTCCACCCGCAGATAGCCGTTCATTATGTTCGGCCCTTTCAGTTGCAGGCGTCCGCCCTCTTCGATACCAGGGACCGACAACAGGCGCGCATCCATTCCTGGTAGAATACGCCCTACCGTACCGGGTTTCGCCGCCATCGGTACGTTGATAGAAACGACAGGCGCGCATTCGGTCACGCCGTAGCCTTCAAGGATGCGCAGGCCAAATTTATCCTGCCAAAGCTGTTTGGTACTTTCTTGTAATTTTTCTGCGCCTGCCACCACATAGCGTAGACGATAGAAGTCATACGGGTTGGCGAAACGCGCGTAGTGACCGAGGAAAGTCGAGGTGCCGAACA  >  NC_000913/2974152‑2974639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑