Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,967,217	C→T	Q406Q (CAG→CAA)	ptsP ←	phosphoenolpyruvate‑protein phosphotransferase PtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,967,217	0	C	T	100.0%	156.0 / NA	48	Q406Q (CAG→CAA)	ptsP	phosphoenolpyruvate‑protein phosphotransferase PtsP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/25);  total (23/25)

GCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACAC‑CGACTAAGCGATCCTGGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCA  >  NC_000913/2966976‑2967458                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   gcaTCCCCTGATACTGCGCCTCCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCt                                                                                                                                                                                                                                           <  1:338416/251‑1 (MQ=255)  caTCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAAGGAGCCGTTGATATTCtt                                                                                                                                                                                                                                          <  1:299261/251‑1 (MQ=255)     ccccTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTgc                                                                                                                                                                                                                                        >  1:322148/1‑250 (MQ=255)                  gcCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTc                                                                                                                                                                                                                          <  1:391421/251‑1 (MQ=255)                   ccACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTcc                                                                                                                                                                                                                         <  1:413646/251‑1 (MQ=255)                                  ccGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAAt                                                                                                                                                                                                          <  1:15032/251‑1 (MQ=255)                                   cGACGGGTAACCTCTTTTCGACATGCATGGGATTTCAGTGCGATAAAGACCGATGCCATCACTCAGGCTGCCCGGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGCGCATGCCTTTTATACGCTCACCACTTTTTAACTGCGCGGGTAACTTGACGTCCTCTTCCGCCCGACGGCTACGCTCAATCTCTTCACTACTTCGCCGTTGATATTCTTGCAGCCGTCCCGGCTCCGGCTCGACCCGCactt                                                                                                                                                                                                         >  2:415396/1‑248 (MQ=255)                                    gACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTc                                                                                                                                                                                                        <  2:86301/251‑1 (MQ=255)                                           aaCCACTTTGCAGCATGAATGGGCTTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGATTTTTAACTGCGCGGGAAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACcgcg                                                                                                                                                                                                  >  2:95351/1‑250 (MQ=255)                                            aCCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGAt                                                                                                                                                                                                >  1:184838/1‑251 (MQ=255)                                                    gcagcaTGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCCCCGCGATAGCCATCa                                                                                                                                                                                        <  2:327974/251‑1 (MQ=255)                                                     cagcaTGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGCCTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCg                                                                                                                                                                                                                              >  1:417377/1‑212 (MQ=255)                                                     cagcaTGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCGCCGCTTTGTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTTAGCTCAAGCTCTTCACTAATTAGCCGTTGATATTCGTGCAGCAGTACCg                                                                                                                                                                                                                              <  2:417377/212‑1 (MQ=255)                                                                 gATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTcc                                                                                                                                                                           <  2:322148/251‑1 (MQ=255)                                                                        gTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATg                                                                                                                                                                    <  1:138928/251‑1 (MQ=255)                                                                          gCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATgca                                                                                                                                                                  <  2:127114/251‑1 (MQ=255)                                                                          gCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCACCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATgca                                                                                                                                                                  <  1:305282/251‑1 (MQ=255)                                                                            gATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATgcagc                                                                                                                                                                >  1:180269/1‑251 (MQ=255)                                                                                                aTACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACAGAAGGCTGAATATCcgc                                                                                                                                            >  2:337402/1‑251 (MQ=255)                                                                                                   cGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACAAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCCGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGCCCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACAGCAGGCTGAATATCCGCGcc                                                                                                                                         >  2:238726/1‑251 (MQ=255)                                                                                                      cTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCCTGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCCGCACCGAAGGCTGAATATCCGCGCCCAt                                                                                                                                      >  2:135821/1‑251 (MQ=255)                                                                                                        gCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATAGTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAACTCACCGCGATAGCTATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATc                                                                                                                                     <  2:31450/250‑1 (MQ=255)                                                                                                        gCCCAGTTTTTCTTCAGGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACGGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCAt                                                                                                                                      <  2:350715/249‑1 (MQ=255)                                                                                                                       aTGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTg                                                                                                                                                     <  1:394406/219‑1 (MQ=255)                                                                                                                         gTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAgc                                                                                                                   >  1:350664/1‑251 (MQ=255)                                                                                                                           tCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTCGGAATCCCCAgcgc                                                                                                                 >  2:118236/1‑251 (MQ=255)                                                                                                                             cGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCGCACCGCTTTTTAACTGCGCGGGGAAATTGACTTCATCTTCCGCCAGGCGGCTAAGCTCAATCCCGTCACTAATTAGCCGTTGGTATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAGTTCACCGCGATAGCCATCAGCGATCGGCGGCCTACGATGCAGCACCGAAGGCTGAATGTCCGCGCCCATCACGGTAGGGAGCGCCAGCGCAc                                                                                                               <  2:189587/251‑1 (MQ=255)                                                                                                                                gCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGt                                                                                                             >  2:290643/1‑250 (MQ=255)                                                                                                                                   tAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCGTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTAc                                                                                                           <  1:346666/249‑1 (MQ=255)                                                                                                                                           cattgcgcataaccattATACGCTCACCGCTTTTTAACTGCGCGGGCAAATTGACGCCCTCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCACTTCACCGCGATAGCCATCACCGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCCCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCgc                                                                                                  >  2:200258/16‑250 (MQ=255)                                                                                                                                                 gCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATgg                                                                                           >  1:159373/1‑251 (MQ=255)                                                                                                                                                                gCTCACCGATTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCa                                                                            <  1:308142/251‑1 (MQ=255)                                                                                                                                                                     ccGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGAGCGCAGCATGGTAGTTGGCTGCGCCATCTCg                                                                       <  1:322696/251‑1 (MQ=255)                                                                                                                                                                           tttAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGac                                                                 >  1:66426/1‑251 (MQ=255)                                                                                                                                                                             tAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGaca                                                                >  1:228059/1‑250 (MQ=255)                                                                                                                                                                              aaCTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGacaac                                                              <  2:101405/251‑1 (MQ=255)                                                                                                                                                                                              ggACGTCATCTTCCGCCAGACGGTTAAGCTCAATCTCTTGACTAAGTAGCCGTTGATATGCGGGCAGCAGTGCCGGCTCCGGAGCGACCAGCAACTCACCGTGATAGTCATCAACGATCAGCGGCCAACGATGCAGCACAGAAGGCCGAATATCCGCGCCCATCACGGTAGGGATACCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACAC‑CGACTAAGCGATcc                                             <  1:415396/250‑1 (MQ=255)                                                                                                                                                                                                         tCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCAACGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCCTGGGAGTTGGCTGCGCCATCTCGCACGACACCAC‑CGACTAAGCGATCCTggggaaactc                                  >  1:272977/1‑245 (MQ=255)                                                                                                                                                                                                          ccGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGAGCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACAC‑CGACTAAGCGATCCTGGGGCAGCTCa                                 <  2:180269/251‑1 (MQ=255)                                                                                                                                                                                                                  cGGCTACGCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATATCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACA‑CACACGACTAAGCGATACTGGGGCAGCTCAGCAAGCGt                         >  2:123572/1‑251 (MQ=255)                                                                                                                                                                                                                         gCTCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACAC‑CGACTAAGCGATCCTGGGGCAGCTCAGCAAGCGTTGTCGCt                  <  1:171443/251‑1 (MQ=255)                                                                                                                                                                                                                           tCAATCTCTTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACAC‑CGACTAAGCGATCCTGGGGCAGCTCAGCAAGCGTTGTCGCTg                 >  1:172768/1‑250 (MQ=255)                                                                                                                                                                                                                                ctctTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACAC‑CGACTAAGCGATCCTGGGGCAGCTCAGCAAGCGTTGTCGCTGACAGtt           >  1:322883/1‑251 (MQ=255)                                                                                                                                                                                                                                 tctTCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACAC‑CGACTAAGCGATCCTGGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTc          >  1:149809/1‑251 (MQ=255)                                                                                                                                                                                                                                   ttCACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGCCAACAC‑CGACTAAGCGATCCTGGGGCAGCTCAGAACGCGTTGTCGCTGACAGTTCAt        >  2:18235/1‑251 (MQ=255)                                                                                                                                                                                                                                     cACTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACAC‑CGACTAAGCGATCCTGGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCt      >  2:15861/1‑251 (MQ=255)                                                                                                                                                                                                                                      aCTAATTAGCCGTTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGAGCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACAC‑CGACTAAGCGATCCTGGGGCAGCTCAGCAAGCGTTGTCGCGGACAGTTGATCTg     <  2:172768/251‑1 (MQ=255)                                                                                                                                                                                                                                         aaTTTGCCGTTGATATTCTTGCAGCAGTACCGGCCCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACAC‑CGACTAAGCGATCCTGGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGcca  <  2:351594/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   GCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAATACGGCTGCCCAGTTTTTCTTCATGTTCCGGGCTTAAACCAGCATTGAGCATGACTTTTATACGCTCACCGCTTTTTAACTGCGCGGGTAAATTGACGTCATCTTCCGCCAGACGGCTAAGCTCAATCTCTTCACTAATTAGCCGCTGATATTCTTGCAGCAGTACCGGCTCCGGATCGACCAGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACAC‑CGACTAAGCGATCCTGGGGCAGCTCAGCAAGCGTTGTCGCTGACAGTTCATCTGCCA  >  NC_000913/2966976‑2967458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑