Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,896,144	G→A	L138L (CTG→TTG)	ygcR ←	putative flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,896,144	0	G	A	97.9%	151.1 / NA	49	L138L (CTG→TTG)	ygcR	putative flavoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (17/30);  minor base C (1/0);  total (18/31)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

GCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCGCAGCCATTCGCTGGCGCATTCCCGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGCAATACAGCTTCCTCAAACCCAAGAGCCATGAGATAGCGCAGCCAGTGCAATGCCCGTTCATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCG  >  NC_000913/2895903‑2896385                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  gCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCGCAGCCATTCGCTGGCGCATTCCCGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATGTCCGCCAACAAAAATgg                                                                                                                                                                                                                                          <  1:343459/251‑1 (MQ=255)   tGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCGCAGCCATTCGCTGGCGCATTCCAGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCg                                                                                                                                                                                                                                        >  1:203255/1‑251 (MQ=255)      ccgttCGGCAGCAACAGTTTTACGGTTAATTTCTGCTTTCCCCGCAGCCATTCGCTGGCGCATTCCCGGGACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGGTCAGTGCGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGTAGCAGGGCCAGCCCAGCATTTCCGCCATCAAAAATGGCGttg                                                                                                                                                                                                                                     <  2:384367/248‑2 (MQ=255)      cATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTGCCCCGCAGCCATTCGCTGGCGCATTCCCGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGttt                                                                                                                                                                                                                                     <  2:343221/251‑1 (MQ=255)          tCGGCAGCCACAGTTTTACGGATAATTTCTGCCTTCCCCGCATCCATTCGCTTGCGCATTCCCGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGGTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGcc                                                                                                                                                                                                                                  <  2:253424/250‑1 (MQ=255)           cGGCAGCAACCGTTTTACGGATAATTTCTGCTTTCCCCGCAGCCATTCGCTGGCGCATTCCCGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAACCCAGCATTTCCGCCAACAAAAATGGCGTTTGccc                                                                                                                                                                                                                                 >  2:386038/1‑250 (MQ=255)            ggCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCTCAGCGATTCGCTGGCGCATTCCCGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCAt                                                                                                                                                                                                                               <  1:136055/251‑1 (MQ=255)             gcagcaACAGTTTTACGGATAATTTCTGCTTTCCCCGCAGCCATTCGCAGGCGCATTCCCGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCAtt                                                                                                                                                                                                                              >  2:86085/1‑251 (MQ=255)                gcaACAGTTTTTCTGATAATTTCTGCTTTCCCCGCAGCCATTCGCTGGCGCATTCCCGGTCCTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCTAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTg                                                                                                                                                                                                                           <  2:363485/251‑1 (MQ=255)                  aaCAGCTTTACGGATAATTTCTGCTTTCCCCGCAGCCATTCGCTGGCGCATTCCCGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTTGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGcc                                                                                                                                                                                                                         <  2:203255/251‑1 (MQ=255)                      gTTTTACGGATAATTTCTGCTTTCCCCGCAGCCATTCGCTGGCGCATTCCCGGCACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTc                                                                                                                                                                                                                     <  1:132359/251‑1 (MQ=255)                      gTTTTACGGATAATTTCAGCTTTCCCCGCAGCCATTCGCTGGCGCATGCCCGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGTGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTGGGCCAGTCTGCCCTTc                                                                                                                                                                                                                     <  2:180198/251‑1 (MQ=255)                            cGGATAATTTCTGCTTTCCCCGCAGCCATTCGCTGGCGCATTCCCGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCgctgct                                                                                                                                                                                                               >  1:105584/1‑251 (MQ=255)                              gATAATTTCTGCTTTCCCCTCAGCCGTTCGCTGGCGCATTCCCGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCCCCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGGTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCCCCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTTCCCTTCTCTGCttt                                                                                                                                                                                                             <  2:359348/251‑1 (MQ=255)                                        gCTTTCCCCGCAGCCATTCGCTGGCGCATTCCCGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCg                                                                                                                                                                                                     <  1:285958/249‑1 (MQ=255)                                              cccGCAGCCATTCGCTGGCGCATTCCCGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTAt                                                                                                                                                                                             >  2:334493/1‑251 (MQ=255)                                                  cAGCCATTCGCTGGCGCATTCACGCTACTGGCAACGCCCCTTAACCGCACTGCCGCACGGCACTCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGCCGCGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTTGCAGCCGGTAATTATc                                                                                                                                                                                            >  2:190237/1‑248 (MQ=255)                                                         tCGCTGGCGCATTCCCGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTGCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGc                                                                                                                                                                                   <  1:111027/250‑1 (MQ=255)                                                           gCTGGCGCATTCCAGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCgg                                                                                                                                                                                 >  2:188017/1‑250 (MQ=255)                                                            cgggCGCCTTCCCCGTTCGGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCTACAACGCAACCCATGTTCAGTTCGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAAGGGCGTTTGCCCATTCTGCCCTTCGCTGCGTTGGCAGCCGGTAATTATCAGATCCATCGGAt                                                                                                                                                                               <  2:275897/249‑1 (MQ=255)                                                               gcgcCTTCCCGGTCCTGGCAAGGCCACCTCACCGCACCGACGGACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGGCGAGAGTGAAACGCTCCACCTGGGTGAAGCCGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCTATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCt                                                                                                                                                                            <  2:324853/251‑1 (MQ=255)                                                                 gcATTCCCGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTg                                                                                                                                                                           >  2:386862/1‑250 (MQ=255)                                                                      cccGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGGAATTATCAGATCCAGCGGATTCTGAtgct                                                                                                                                                                      <  1:128669/250‑1 (MQ=255)                                                                            aCTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCATGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTc                                                                                                                                                               <  1:204207/251‑1 (MQ=255)                                                                                   aGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCGGATGCTGCCAGTCGGCAata                                                                                                                                                        <  1:131117/251‑1 (MQ=255)                                                                                            cACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCGGCCATTCGGCAATATGgcgagcg                                                                                                                                               <  1:113105/251‑1 (MQ=255)                                                                                                           cGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTGCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATGTCCGCCAACAAAAATGGCGTTGGCCCATTCTGCCCTGCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGAGTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCa                                                                                                                                <  1:278195/251‑1 (MQ=255)                                                                                                                         ggCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGAAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTgc                                                                                                                  >  1:240508/1‑251 (MQ=255)                                                                                                                                   cccGGCAAGAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTcc                                                                                                        >  2:287711/1‑251 (MQ=255)                                                                                                                                      ggCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGc                                                                                                     <  1:386862/251‑1 (MQ=255)                                                                                                                                                   cccATGTTCAGTACGCTGTTCGAGGGTGATAAACAGAGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGCTTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGCACTACAGCTTCCt                                                                                        >  2:113596/1‑251 (MQ=255)                                                                                                                                                     cATGTTCAGTACGCTGTTCGAGGGGGAGAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCAGTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGTTAATTATCAGATCCAGCGGATTCTGATGCTGCCATGCGGCAAGATGGCGAGCGACAAATTCCGGCGCAAAGCTCAGATCTGCCGCCGTCTCCGGCATTACAGCTTCCTCa                                                                                      <  2:379487/251‑1 (MQ=255)                                                                                                                                                        gTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGCAATACAGCTTCCTCAAAc                                                                                   >  1:235619/1‑251 (MQ=255)                                                                                                                                                            gttgcGCTGTTCGAGGGTGATAACCAGCGCGTCGAGTGGGAACCGCTCCACCGGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCGGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGAGTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAAGTCCGGCGCAAAGCGCATATCTGCCGTCGTCGCCAGCAATGCAGTTTCCTCaagcctaa                                                                               <  2:294714/247‑7 (MQ=255)                                                                                                                                                                 gCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGCAATACAGCTTCCTCAAACCCAAGAGcc                                                                          >  1:281334/1‑251 (MQ=255)                                                                                                                                                                    gTTCGAGGGTGATAAACCGCGCGTCGAGAGTGAAACGATCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCACCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACCAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGCAATACAGCTTCCTCAAACCCAAGAGCCATg                                                                       >  2:252919/1‑251 (MQ=255)                                                                                                                                                                        gAGGGGGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCTGATTCTGATGCTGCCAGTCGGCAATATGGCGAGCGACAAATGCCGGCGCAAATCGCAGATCGGCCGCCGTCTCCAGCAATACAGCTTCCTCAAACCCAAGAGCCTTGAGAt                                                                   <  2:177497/251‑1 (MQ=255)                                                                                                                                                                              gATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGATGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAAGGGCGTATGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCATATCTGCCGCCGTCTCCAGCAATACAGCTTCCTCAAACCCAAGAGCCATGAGATAgcgc                                                              <  2:339401/250‑1 (MQ=255)                                                                                                                                                                                 aaaCAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCCGCATTTCCGCCAACAAGACTGGAGTTTGCCCATTCTGCACTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGAGCACAGCGCAGATCTGCCGCCGTCTACAGCAATAAAGCTTCCTCCAACCCCAGAGCCATGAAATAGCGCAGcc                                                          >  1:207679/1‑251 (MQ=255)                                                                                                                                                                                      gcgcGGCGAGAGTGAAACGCTCCCCCTGGGTGAAGCAGGGCCAGCCCAGCATGTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGCAATACAGCTTCCTCAAACCCAAGAGCCATTAGATAGCGCAGCCAGTGc                                                     <  2:149750/251‑1 (MQ=255)                                                                                                                                                                                                   gAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCCACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGCAATACAGCTTCCTCAAACCCACGAGCCATGAGATAGCGCAGCCAGTGCAATGCCCGTTCAt                                        >  2:265355/1‑251 (MQ=255)                                                                                                                                                                                                           ccgccTGGGTGAAGCAGGGCCATCACAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGCAATACAGCTTCCTCAAGCCCAAGAGCCATGAGATATCGCAGCCAGTGCAATGCCCGTTCATCCCCCAt                                 <  1:90978/247‑1 (MQ=255)                                                                                                                                                                                                           ccACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGCAATACAGCTTCCTCAAACCCAAGAGCCATGAGATAGCGCAGCCAGTGCAATGCCCGTTCATCCCCCa                                  <  1:265355/249‑1 (MQ=255)                                                                                                                                                                                                             cccTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGCAATACAGCTTCCTCAAACCCAAGAGCCATGAGATAGCGCAGCCAGTGCAATGCCCGTTCATCCCCCATGCt                              <  2:371683/250‑1 (MQ=255)                                                                                                                                                                                                                tGGGTGAAGCAGAGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATACGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGCAATACAGCTTCCTCAAACCCAAGAGCCATGAGATAGCGCAGCCAGTGCAATGCCCGTTCATCCCCCATGCTCa                            >  1:417649/1‑250 (MQ=255)                                                                                                                                                                                                                          aGGGCCAGCCCAGCATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGCAATACAGCTTCCTCAAACCCAAGAGCCATGAGATAGCGCAGCCAGTGCAATGCCCGTTCATCCCCCATGCTCAACGCGGTTaa                  >  1:220218/1‑250 (MQ=255)                                                                                                                                                                                                                               cagcccagcATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGAGCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGCAATACAGCTTCCGCAAACCCAAGAGCCATGAGATAGCGCAGCCAGTGCAATGCCCGTTCATCCCCCATGCTCAACGCGGTTAAAGACAt            <  1:93909/251‑1 (MQ=255)                                                                                                                                                                                                                                gtcccagcATTTCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATGCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGCAATACAGCTTCCTCAAACCCAAGAGCCATGAGAGAGCGCAGCCAGTGCAATGCCCGTTCATCCCCCATGCTCAACGCGGTTAAAGACATc           <  2:104556/249‑1 (MQ=255)                                                                                                                                                                                                                                          ttCCGCCAACAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGCAATACAGCTTCCTCAAACCCAAGAGCCATGAGATAGCGCAGCCAGTGCAATGCCCGTTCATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCg  <  1:79295/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  GCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCGCAGCCATTCGCTGGCGCATTCCCGGTACTGGCAAGGCCACTTCACCGCACTGACGCACGGCAATCACCGCAGGCAAGCGAACCCGGCAACAACGCAACCCATGTTCAGTACGCTGTTCGAGGGTGATAAACAGCGCGTCGAGAGTGAAACGCTCCACCTGGGTGAAGCAGGGCCAGCCCAGCATTTCCGCCAGCAAAAATGGCGTTTGCCCATTCTGCCCTTCGCTGCTTTGGCAGCCGGTAATTATCAGATCCAGCGGATTCTGATGCTGCCATTCGGCAATATGGCGAGCGACAAATTCCGGCGCAAAGCGCAGATCTGCCGCCGTCTCCAGCAATACAGCTTCCTCAAACCCAAGAGCCATGAGATAGCGCAGCCAGTGCAATGCCCGTTCATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCG  >  NC_000913/2895903‑2896385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑